Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30125
(712 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 135 7e-33
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 123 2e-29
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 74 2e-14
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 59 7e-10
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 33 0.053
SPBC1289.01c |chr4|cfh3, SPBC1539.11c|chitin synthase regulatory... 27 3.5
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.1
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 135 bits (326), Expect = 7e-33
Identities = 58/85 (68%), Positives = 69/85 (81%)
Frame = +2
Query: 2 GIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFXDLEPTVVDEVRTGTYRQLFH 181
GIQP+G M + D F+TFFSETG GK+VPR+++ DLEP V+D+VRTG YR LFH
Sbjct: 29 GIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFH 88
Query: 182 PEQLITGKEDAANNYARGHYTIGKE 256
PEQLITGKEDA+NNYARGHYT+GKE
Sbjct: 89 PEQLITGKEDASNNYARGHYTVGKE 113
Score = 120 bits (290), Expect = 2e-28
Identities = 55/67 (82%), Positives = 60/67 (89%)
Frame = +3
Query: 510 DCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQNN 689
DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSITASLRF+G+LNVDL EFQ N
Sbjct: 199 DCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTN 258
Query: 690 LVP*PRI 710
LVP PRI
Sbjct: 259 LVPYPRI 265
Score = 102 bits (244), Expect = 6e-23
Identities = 45/77 (58%), Positives = 60/77 (77%)
Frame = +1
Query: 250 KGIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAI 429
K +VD V D+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++
Sbjct: 112 KELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSV 171
Query: 430 YPAPQVSTAVVEPYNSI 480
YPAPQVST+VVEPYNS+
Sbjct: 172 YPAPQVSTSVVEPYNSV 188
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 123 bits (297), Expect = 2e-29
Identities = 57/90 (63%), Positives = 69/90 (76%), Gaps = 5/90 (5%)
Frame = +2
Query: 2 GIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKHVPRAVFXDLEPTVVDEVRTGTY 166
GI PDG PT+ K +D F TFFSETG GK VPR+++ DLEP V+D+VRTG Y
Sbjct: 29 GIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPY 87
Query: 167 RQLFHPEQLITGKEDAANNYARGHYTIGKE 256
+ LFHPEQ++TGKEDA+NNYARGHYT+GKE
Sbjct: 88 KDLFHPEQMVTGKEDASNNYARGHYTVGKE 117
Score = 122 bits (293), Expect = 7e-29
Identities = 57/67 (85%), Positives = 59/67 (88%)
Frame = +3
Query: 510 DCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQNN 689
DC FMVDNEA YDICRRNLDIERPTY NLNRLI Q+VSSITASLRF G+LNVDL EFQ N
Sbjct: 203 DCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTN 262
Query: 690 LVP*PRI 710
LVP PRI
Sbjct: 263 LVPYPRI 269
Score = 104 bits (250), Expect = 1e-23
Identities = 45/77 (58%), Positives = 61/77 (79%)
Frame = +1
Query: 250 KGIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAI 429
K ++D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++
Sbjct: 116 KEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSV 175
Query: 430 YPAPQVSTAVVEPYNSI 480
YPAPQVST+VVEPYNS+
Sbjct: 176 YPAPQVSTSVVEPYNSV 192
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 74.1 bits (174), Expect = 2e-14
Identities = 31/67 (46%), Positives = 44/67 (65%)
Frame = +2
Query: 56 DSFNTFFSETGAGKHVPRAVFXDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARG 235
+ N +F+E GK+VPRAV DLEP +D V++G + LF P+ +I G+ A N +A+G
Sbjct: 45 ERLNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKG 104
Query: 236 HYTIGKE 256
HYT G E
Sbjct: 105 HYTEGAE 111
Score = 62.5 bits (145), Expect = 6e-11
Identities = 28/67 (41%), Positives = 41/67 (61%)
Frame = +3
Query: 510 DCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQNN 689
D F +DNEA+ I L I+ P+Y +LN L+ +++ +T S RF G LN DL + N
Sbjct: 197 DETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVN 256
Query: 690 LVP*PRI 710
+VP PR+
Sbjct: 257 MVPFPRL 263
Score = 57.2 bits (132), Expect = 2e-09
Identities = 28/81 (34%), Positives = 44/81 (54%), Gaps = 2/81 (2%)
Frame = +1
Query: 241 HHWKG--IVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSK 414
H+ +G + D VLD +R+ A+ C LQGF + H LL+ ++ +Y +
Sbjct: 105 HYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMM 164
Query: 415 LEFAIYPAPQVSTAVVEPYNS 477
F++ PAP+ S VVEPYN+
Sbjct: 165 ATFSVAPAPKSSDTVVEPYNA 185
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 58.8 bits (136), Expect = 7e-10
Identities = 30/81 (37%), Positives = 48/81 (59%), Gaps = 2/81 (2%)
Frame = +2
Query: 2 GIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFXDLEPTVVDEVRTGTYRQLFH 181
GI PDG + + T G D + FF ++ +++PRA+ DLEP VV+ + + TY L++
Sbjct: 30 GIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYGSLYN 87
Query: 182 PEQLITGKE--DAANNYARGH 238
PE ++ K A NN+A G+
Sbjct: 88 PENILITKNGGGAGNNWANGY 108
Score = 48.4 bits (110), Expect = 1e-06
Identities = 27/80 (33%), Positives = 42/80 (52%), Gaps = 1/80 (1%)
Frame = +1
Query: 244 HWKGIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEF 423
H + I + ++D I + AD L+GF + H L+ERL+ Y KK +
Sbjct: 110 HAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTY 169
Query: 424 AIYPAPQ-VSTAVVEPYNSI 480
+++P Q VS VV+PYNS+
Sbjct: 170 SVFPNSQSVSDVVVQPYNSL 189
Score = 48.4 bits (110), Expect = 1e-06
Identities = 22/66 (33%), Positives = 37/66 (56%)
Frame = +3
Query: 510 DCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQNN 689
D ++DN A+ I L + PT+ N+L+ ++S+ T +LR+ G +N DL +
Sbjct: 200 DSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIAS 259
Query: 690 LVP*PR 707
L+P PR
Sbjct: 260 LIPSPR 265
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 32.7 bits (71), Expect = 0.053
Identities = 17/48 (35%), Positives = 28/48 (58%)
Frame = -3
Query: 680 ELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDG 537
E + H ++ + Q+SSD ++ +D T VG+G AL I + D +DG
Sbjct: 171 ERAKEHRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
>SPBC1289.01c |chr4|cfh3, SPBC1539.11c|chitin synthase regulatory
factor |Schizosaccharomyces pombe|chr 2|||Manual
Length = 633
Score = 26.6 bits (56), Expect = 3.5
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +1
Query: 235 SLHHWKGIVDLVLDRIRKLADQCTGLQGF 321
+LH G+VDL + IR D+ T L+ F
Sbjct: 401 NLHEHPGLVDLGSENIRVPIDEATALKSF 429
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 6.1
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 16 WPDAHRQDHRGWRRFFQHFLQR 81
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,880,308
Number of Sequences: 5004
Number of extensions: 55764
Number of successful extensions: 173
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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