Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30117
(751 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 144 2e-35
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 144 2e-35
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 144 2e-35
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 54 3e-08
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 35 0.011
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 32 0.10
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 3.8
SPAC25H1.07 |||DUF1620 family protein|Schizosaccharomyces pombe|... 26 5.0
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 6.6
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 8.7
SPAC227.12 |||U4/U6 x U5 tri-snRNP complex subunit Prp4 family|S... 25 8.7
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 144 bits (348), Expect = 2e-35
Identities = 65/84 (77%), Positives = 72/84 (85%)
Frame = +2
Query: 2 KPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNN 181
KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+
Sbjct: 271 KPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKND 330
Query: 182 PPKGAADFTAQVIVLNHPGQISNG 253
PP G A FTAQVI+LNHPGQIS G
Sbjct: 331 PPMGCASFTAQVIILNHPGQISAG 354
Score = 143 bits (346), Expect = 3e-35
Identities = 63/85 (74%), Positives = 75/85 (88%)
Frame = +1
Query: 253 FTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQ 432
++PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F
Sbjct: 355 YSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFT 414
Query: 433 EFPPLGRFAVRDMRQTVAVGVIKAV 507
++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 415 DYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 144 bits (348), Expect = 2e-35
Identities = 65/84 (77%), Positives = 72/84 (85%)
Frame = +2
Query: 2 KPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNN 181
KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+
Sbjct: 271 KPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKND 330
Query: 182 PPKGAADFTAQVIVLNHPGQISNG 253
PP G A FTAQVI+LNHPGQIS G
Sbjct: 331 PPMGCASFTAQVIILNHPGQISAG 354
Score = 143 bits (346), Expect = 3e-35
Identities = 63/85 (74%), Positives = 75/85 (88%)
Frame = +1
Query: 253 FTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQ 432
++PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F
Sbjct: 355 YSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFT 414
Query: 433 EFPPLGRFAVRDMRQTVAVGVIKAV 507
++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 415 DYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 144 bits (348), Expect = 2e-35
Identities = 65/84 (77%), Positives = 72/84 (85%)
Frame = +2
Query: 2 KPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNN 181
KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+
Sbjct: 271 KPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKND 330
Query: 182 PPKGAADFTAQVIVLNHPGQISNG 253
PP G A FTAQVI+LNHPGQIS G
Sbjct: 331 PPMGCASFTAQVIILNHPGQISAG 354
Score = 143 bits (346), Expect = 3e-35
Identities = 63/85 (74%), Positives = 75/85 (88%)
Frame = +1
Query: 253 FTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQ 432
++PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F
Sbjct: 355 YSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFT 414
Query: 433 EFPPLGRFAVRDMRQTVAVGVIKAV 507
++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 415 DYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 53.6 bits (123), Expect = 3e-08
Identities = 27/82 (32%), Positives = 45/82 (54%)
Frame = +1
Query: 253 FTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQ 432
++ V+ HTA FA++ K+D+ T + ++ P G I L P+C+E F+
Sbjct: 577 YSCVMHIHTAVEEVSFAKLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFE 635
Query: 433 EFPPLGRFAVRDMRQTVAVGVI 498
++ +GRF +RD TVAVG +
Sbjct: 636 DYQYMGRFTLRDQGTTVAVGKV 657
Score = 37.1 bits (82), Expect = 0.003
Identities = 24/85 (28%), Positives = 41/85 (48%), Gaps = 1/85 (1%)
Frame = +2
Query: 2 KPGTIVVFAPANITTEVKSV-EMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKN 178
K + V+ P N T EV ++ + E + ++ GD V V+ +++ GYV +KN
Sbjct: 494 KKNSNVLVMPINQTLEVTAIYDEADEEISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN 552
Query: 179 NPPKGAADFTAQVIVLNHPGQISNG 253
P F AQ+ +L P ++ G
Sbjct: 553 -PVHATTRFIAQIAILELPSILTTG 576
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 35.1 bits (77), Expect = 0.011
Identities = 14/43 (32%), Positives = 27/43 (62%)
Frame = +2
Query: 32 ANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYV 160
+++ T V +EM + L AV GDN G ++++ ++L+RG +
Sbjct: 292 SHLKTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMI 334
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 31.9 bits (69), Expect = 0.10
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = +1
Query: 409 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 501
PLC+ +E P LGRF +R TVA G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 3.8
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -1
Query: 145 QFLDGHVLYVETYIVSRYSFLESFV 71
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC25H1.07 |||DUF1620 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 885
Score = 26.2 bits (55), Expect = 5.0
Identities = 12/35 (34%), Positives = 21/35 (60%)
Frame = +2
Query: 566 QEVARAVNSTIFHTTAILHSPKSVSKEKRATNSFL 670
+ +A A+N +I TT + KS+ E+ ++ SFL
Sbjct: 369 EALAYAINPSILPTTLLTSYQKSIQDEENSSVSFL 403
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 25.8 bits (54), Expect = 6.6
Identities = 10/39 (25%), Positives = 22/39 (56%)
Frame = -1
Query: 169 VTSNITTTQFLDGHVLYVETYIVSRYSFLESFVVHLHRF 53
+T ++ + L H+ +++ SR +FL ++H+H F
Sbjct: 1012 LTQSLQSFSHLSNHIEVLDSTRQSRLTFLCHLILHMHGF 1050
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 8.7
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = -3
Query: 386 MAASPDLMDFGLTSVDLPVRRSTFS 312
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
>SPAC227.12 |||U4/U6 x U5 tri-snRNP complex subunit Prp4
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 462
Score = 25.4 bits (53), Expect = 8.7
Identities = 9/22 (40%), Positives = 13/22 (59%)
Frame = -3
Query: 311 LISANLQAMWAVWQSNTGVNRL 246
L+SA+ W +W +TGV L
Sbjct: 280 LVSASFDTTWRLWDVHTGVELL 301
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,131,377
Number of Sequences: 5004
Number of extensions: 64861
Number of successful extensions: 192
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 191
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -