Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30103
(702 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g18170.1 68418.m02133 glutamate dehydrogenase 1 (GDH1) identi... 78 7e-15
At5g07440.1 68418.m00851 glutamate dehydrogenase 2 (GDH2) identi... 77 9e-15
At3g03910.1 68416.m00405 glutamate dehydrogenase, putative simil... 77 2e-14
At1g51720.1 68414.m05828 glutamate dehydrogenase, putative simil... 34 0.10
At5g58670.1 68418.m07351 phosphoinositide-specific phospholipase... 31 0.56
At3g11430.1 68416.m01394 phospholipid/glycerol acyltransferase f... 30 1.7
At1g03990.1 68414.m00385 alcohol oxidase-related low similarity ... 29 2.3
At1g02120.1 68414.m00138 GRAM domain-containing protein-related ... 29 3.0
At5g03860.1 68418.m00358 malate synthase, putative strong simila... 27 9.1
At4g09820.1 68417.m01611 basic helix-loop-helix (bHLH) family pr... 27 9.1
At3g10650.1 68416.m01281 expressed protein 27 9.1
At2g32800.1 68415.m04015 protein kinase family protein contains ... 27 9.1
At1g09020.1 68414.m01006 protein kinase, putative similar to pro... 27 9.1
>At5g18170.1 68418.m02133 glutamate dehydrogenase 1 (GDH1) identical
to glutamate dehydrogenase 1 (GDH 1) [Arabidopsis
thaliana] SWISS-PROT:Q43314
Length = 411
Score = 77.8 bits (183), Expect = 7e-15
Identities = 31/57 (54%), Positives = 47/57 (82%)
Frame = +2
Query: 254 QDNAHRIQAKIIAEAANGPTTPAADKILIDRNILVIPDLYINAGGVTVSFFEWLKNL 424
++NA+ I+AK I EAAN PT P AD+IL + ++++PD+Y N+GGVTVS+FEW++N+
Sbjct: 296 RENANEIKAKFIIEAANHPTDPDADEILSKKGVVILPDIYANSGGVTVSYFEWVQNI 352
Score = 40.3 bits (90), Expect = 0.001
Identities = 20/36 (55%), Positives = 24/36 (66%)
Frame = +3
Query: 150 RYVVGFPGAKAYEGENMLYEKCDILVPAAIEQVINR 257
R V GF GA + ++L E CDILVPAA+ VINR
Sbjct: 261 RGVKGFDGADPIDPNSILVEDCDILVPAALGGVINR 296
Score = 37.1 bits (82), Expect = 0.011
Identities = 18/50 (36%), Positives = 28/50 (56%)
Frame = +1
Query: 1 VQGFGNVGLHTCRYLVRAGATCIGVIEHDGSIYNPDGINPKALEEYRIEN 150
+QGFGNVG + + G + V + G+I N DGI+ AL ++ E+
Sbjct: 211 IQGFGNVGSWAAKLISEKGGKIVAVSDITGAIKNKDGIDIPALLKHTKEH 260
>At5g07440.1 68418.m00851 glutamate dehydrogenase 2 (GDH2) identical
to glutamate dehydrogenase 2 (GDH 2) [Arabidopsis
thaliana] SWISS-PROT:Q38946
Length = 411
Score = 77.4 bits (182), Expect = 9e-15
Identities = 31/57 (54%), Positives = 46/57 (80%)
Frame = +2
Query: 254 QDNAHRIQAKIIAEAANGPTTPAADKILIDRNILVIPDLYINAGGVTVSFFEWLKNL 424
++NA ++AK I EAAN PT P AD+IL + ++++PD+Y NAGGVTVS+FEW++N+
Sbjct: 296 KENAGDVKAKFIVEAANHPTDPDADEILSKKGVIILPDIYANAGGVTVSYFEWVQNI 352
Score = 40.3 bits (90), Expect = 0.001
Identities = 18/52 (34%), Positives = 30/52 (57%)
Frame = +1
Query: 1 VQGFGNVGLHTCRYLVRAGATCIGVIEHDGSIYNPDGINPKALEEYRIENGT 156
+QGFGNVG + + G + V + G+I NP+GI+ AL +++ G+
Sbjct: 211 IQGFGNVGTWAAKLIHEKGGKVVAVSDITGAIRNPEGIDINALIKHKDATGS 262
Score = 31.5 bits (68), Expect = 0.56
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = +3
Query: 165 FPGAKAYEGENMLYEKCDILVPAAIEQVINR 257
F G A + +L +CD+L+P A+ V+N+
Sbjct: 266 FNGGDAMNSDELLIHECDVLIPCALGGVLNK 296
>At3g03910.1 68416.m00405 glutamate dehydrogenase, putative similar
to glutamate dehydrogenase 1 (GDH 1) [Arabidopsis
thaliana] SWISS-PROT:Q43314
Length = 411
Score = 76.6 bits (180), Expect = 2e-14
Identities = 30/57 (52%), Positives = 46/57 (80%)
Frame = +2
Query: 254 QDNAHRIQAKIIAEAANGPTTPAADKILIDRNILVIPDLYINAGGVTVSFFEWLKNL 424
++NA+ I+AK I E AN PT P AD+IL + ++++PD+Y N+GGVTVS+FEW++N+
Sbjct: 296 RENANEIKAKFIIEGANHPTDPEADEILKKKGVMILPDIYANSGGVTVSYFEWVQNI 352
Score = 41.5 bits (93), Expect = 5e-04
Identities = 19/36 (52%), Positives = 26/36 (72%)
Frame = +3
Query: 150 RYVVGFPGAKAYEGENMLYEKCDILVPAAIEQVINR 257
R + GF GA + + +++L E CDILVPAA+ VINR
Sbjct: 261 RGIKGFDGADSIDPDSILVEDCDILVPAALGGVINR 296
Score = 35.9 bits (79), Expect = 0.026
Identities = 18/50 (36%), Positives = 28/50 (56%)
Frame = +1
Query: 1 VQGFGNVGLHTCRYLVRAGATCIGVIEHDGSIYNPDGINPKALEEYRIEN 150
+QGFGNVG + + G + V + G+I N +GI+ +L E+ EN
Sbjct: 211 IQGFGNVGSWAAKLISDKGGKIVAVSDVTGAIKNNNGIDILSLLEHAEEN 260
>At1g51720.1 68414.m05828 glutamate dehydrogenase, putative similar
to NADP-specific glutatamate dehydrogenase (NADP-GDH)
SP:P28724 [Giardia lamblia (Giardia intestinalis)]
Length = 637
Score = 33.9 bits (74), Expect = 0.10
Identities = 16/59 (27%), Positives = 28/59 (47%)
Frame = +2
Query: 281 KIIAEAANGPTTPAADKILIDRNILVIPDLYINAGGVTVSFFEWLKNLNHVSYGRLTFK 457
+++ E +N P T A + N+L+ P + AGGV E L+ N + + F+
Sbjct: 526 RLLVEGSNMPCTAEAVDVFRKANVLIAPAIAAGAGGVAAGEIEVLRESNSMQWSAEDFE 584
>At5g58670.1 68418.m07351 phosphoinositide-specific phospholipase C
(PLC1) identical to phosphoinositide specific
phospholipase C [Arabidopsis thaliana] GI:902923
Length = 561
Score = 31.5 bits (68), Expect = 0.56
Identities = 17/40 (42%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = -3
Query: 373 VEVGDHENVAID-EDLVCSRCGGTVGGLRNDLSLDPVSVV 257
V+ D E+VAI+ DL+ G GGL+N L+ DP V+
Sbjct: 281 VQEEDEESVAIEYRDLISIHAGNRKGGLKNCLNGDPNRVI 320
>At3g11430.1 68416.m01394 phospholipid/glycerol acyltransferase
family protein contains Pfam profile: PF01553
acyltransferase
Length = 502
Score = 29.9 bits (64), Expect = 1.7
Identities = 17/40 (42%), Positives = 22/40 (55%)
Frame = -2
Query: 257 PVYDLFDGGGYKNVALLVKHVLAFVGLSAGETDYVPFSIL 138
PV L D YKN AL +K +A VGL E + V ++L
Sbjct: 52 PVITLLDVFSYKNAALKLKIFVATVGLREPEIESVARAVL 91
>At1g03990.1 68414.m00385 alcohol oxidase-related low similarity to
long chain fatty alcohol oxidase from Candida cloacae
[GI:6983581], Candida tropicalis [GI:6983594]; Location
of EST 248L9T7, gb|AA713296
Length = 758
Score = 29.5 bits (63), Expect = 2.3
Identities = 17/41 (41%), Positives = 20/41 (48%), Gaps = 1/41 (2%)
Frame = +1
Query: 19 VGL-HTCRYLVRAGATCIGVIEHDGSIYNPDGINPKALEEY 138
+GL R LV AGA +G DG DGI K LE +
Sbjct: 625 IGLKQALRILVAAGAAEVGTYRSDGQRMKCDGIKQKDLEAF 665
>At1g02120.1 68414.m00138 GRAM domain-containing protein-related
contains low similarity to PF02893: GRAM domain
Length = 556
Score = 29.1 bits (62), Expect = 3.0
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +3
Query: 531 RPHPRHSLRVLPEENLRRLREGHRALRTRLHHGE 632
R H ++ E+ L+R+R+ H AL+ + HH E
Sbjct: 515 RMHFLREEMMMVEDRLQRMRQDHAALKAQFHHLE 548
>At5g03860.1 68418.m00358 malate synthase, putative strong
similarity to glyoxysomal malate synthase from Brassica
napus [SP|P13244]
Length = 562
Score = 27.5 bits (58), Expect = 9.1
Identities = 13/24 (54%), Positives = 16/24 (66%)
Frame = +1
Query: 457 IREGI*LPSAGIGPRVSQRRFGRV 528
IR G+ L G+G RVS+ FGRV
Sbjct: 478 IRYGVELDGDGLGVRVSKELFGRV 501
>At4g09820.1 68417.m01611 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain
Length = 379
Score = 27.5 bits (58), Expect = 9.1
Identities = 14/60 (23%), Positives = 30/60 (50%), Gaps = 2/60 (3%)
Frame = -3
Query: 526 RDRTAAERLLDRFQQMVVRFPLVFECQTSVRH--VIEVLEPLEE*DGDTTSVDVEVGDHE 353
R RT + + + ++ ++ E + R ++++L+ L E +TT+V V DH+
Sbjct: 287 RTRTCKRKTSEEVEVSIIENDVLLEMRCEYRDGLLLDILQVLHELGIETTAVHTSVNDHD 346
>At3g10650.1 68416.m01281 expressed protein
Length = 1309
Score = 27.5 bits (58), Expect = 9.1
Identities = 15/38 (39%), Positives = 19/38 (50%)
Frame = +1
Query: 166 SPALRPTKARTCFTRSATFLYPPPSNRS*TGQRSQDPS 279
S + PT + F+ A + PPPSN S T S PS
Sbjct: 737 STSAAPTLNGSIFSAGANAVTPPPSNGSLTSSPSFPPS 774
>At2g32800.1 68415.m04015 protein kinase family protein contains
dual protein kinase domains, Pfam:PF00069
Length = 851
Score = 27.5 bits (58), Expect = 9.1
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = +1
Query: 523 RVGGRIPVTPSESFQKRISGASEKDIVHSGL 615
R+GG I P ESF+K+ ++ D+ G+
Sbjct: 304 RIGGTIGYLPPESFRKKTVATAKTDVFSFGV 334
>At1g09020.1 68414.m01006 protein kinase, putative similar to
protein kinase AKINbetagamma-2 [Zea mays] GI:11139548,
AKINbetagamma-1 [Zea mays] GI:11139546; contains Pfam
profile PF00571: CBS domain
Length = 487
Score = 27.5 bits (58), Expect = 9.1
Identities = 13/37 (35%), Positives = 20/37 (54%), Gaps = 1/37 (2%)
Frame = +3
Query: 474 TTICWNRSKS-LSAAVRSRGRPHPRHSLRVLPEENLR 581
T W K+ +S GRP+PR ++V P +NL+
Sbjct: 238 TIAAWKEGKAHISRQYDGSGRPYPRPLVQVGPYDNLK 274
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,877,640
Number of Sequences: 28952
Number of extensions: 310495
Number of successful extensions: 914
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 890
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 914
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1506636208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -