BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30089
(785 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g13520.1 68418.m01561 peptidase M1 family protein similar to ... 78 8e-15
At4g33090.1 68417.m04715 aminopeptidase M similar to SP|Q11011 P... 35 0.053
At4g10710.1 68417.m01751 transcriptional regulator-related simil... 31 0.87
At3g17630.1 68416.m02252 cation/hydrogen exchanger, putative (CH... 31 0.87
At1g68280.1 68414.m07799 thioesterase-related contains domain si... 30 2.0
At3g10740.1 68416.m01293 glycosyl hydrolase family protein 51 si... 28 6.1
At3g44670.1 68416.m04804 disease resistance protein RPP1-Ws[A,C]... 28 8.1
At1g73960.1 68414.m08565 expressed protein similar to TATA bindi... 28 8.1
>At5g13520.1 68418.m01561 peptidase M1 family protein similar to
SP|P09960 Leukotriene A-4 hydrolase (EC 3.3.2.6) (LTA-4
hydrolase) {Homo sapiens}; contains Pfam profile
PF01433: Peptidase family M1
Length = 616
Score = 77.8 bits (183), Expect = 8e-15
Identities = 31/58 (53%), Positives = 39/58 (67%)
Frame = +1
Query: 508 VQWLQPAQTSGKKHPYLFSQCQPIHARSILPCQDTPFVKFTYDAEVTAPEEFTVLMSA 681
+QWL P QT K HPY+++QCQ IHARSI PCQDTP + YD + P + +MSA
Sbjct: 109 LQWLSPLQTFSKLHPYVYTQCQAIHARSIFPCQDTPAARIRYDVVMNIPNSLSAVMSA 166
Score = 29.5 bits (63), Expect = 2.6
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +3
Query: 177 SPLDPSSFSRPEQAVIKHVTLSLNVDLKTKS*TDPPLSTLT 299
+P+DP SF+ + HV LSL +D T L TL+
Sbjct: 2 APIDPHSFTDSSHPLTTHVALSLYLDFNTSIIHGSALLTLS 42
>At4g33090.1 68417.m04715 aminopeptidase M similar to SP|Q11011
Puromycin-sensitive aminopeptidase (EC 3.4.11.-) (PSA)
{Mus musculus}; contains Pfam profile PF01433: Peptidase
family M1
Length = 879
Score = 35.1 bits (77), Expect = 0.053
Identities = 24/80 (30%), Positives = 36/80 (45%), Gaps = 4/80 (5%)
Frame = +1
Query: 535 SGKKHPYLFSQCQPIHARSILPCQDTPFVKFTYDAEVTAPEEFTVLMSALRGESR---ST 705
+G+K +Q +P AR PC D P K T+ + P + L + E + +
Sbjct: 126 NGEKKNMAVTQFEPADARRCFPCWDEPACKATFKITLEVPTDLVALSNMPIMEEKVNGNL 185
Query: 706 KTTFNQPMPLPS-YLLAIAV 762
K Q P+ S YL+AI V
Sbjct: 186 KIVSYQESPIMSTYLVAIVV 205
>At4g10710.1 68417.m01751 transcriptional regulator-related similar
to chromatin-specific transcription elongation factor
FACT 140 kDa subunit (GI:5499741) [Homo sapiens]
Length = 1074
Score = 31.1 bits (67), Expect = 0.87
Identities = 19/36 (52%), Positives = 21/36 (58%)
Frame = +1
Query: 112 TLVYTQNKQGHASVKFQSWVLSVLWIRLPFRGLNKL 219
TLV TQ K A KF+ LS LWIR PF G K+
Sbjct: 661 TLV-TQEKLQLAGNKFKPLRLSELWIRPPFSGRKKI 695
>At3g17630.1 68416.m02252 cation/hydrogen exchanger, putative
(CHX19) similar to putative Na+/H+-exchanging protein
GB:CAA23036 from [Arabidopsis thaliana]; monovalent
cation:proton antiporter family 2 (CPA2) member,
PMID:11500563
Length = 857
Score = 31.1 bits (67), Expect = 0.87
Identities = 17/45 (37%), Positives = 24/45 (53%)
Frame = -1
Query: 461 YHHLRLVSAIV*SIWSHSSALGHLICTSIEHRLVLLILLSVHSNQ 327
Y HLR V+ + S S++ ICTS + V +ILL H +Q
Sbjct: 588 YQHLRAVAVRPMTAISGLSSIHEDICTSAHQKRVAMILLPFHKHQ 632
>At1g68280.1 68414.m07799 thioesterase-related contains domain
similarity with PF03061: thioesterase family protein
Length = 188
Score = 29.9 bits (64), Expect = 2.0
Identities = 14/47 (29%), Positives = 26/47 (55%)
Frame = +2
Query: 416 SKLTIQLPKRASSGDKLKIKIKYTTSPSATAYNGYSQLKLRGRNILI 556
S+LT+ SGDK +K+ + + +A Y +S LKL + +++
Sbjct: 106 SELTMNFLAPLRSGDKFVVKVNISRTSAARIYFDHSILKLPNQEVIL 152
>At3g10740.1 68416.m01293 glycosyl hydrolase family protein 51
similar to arabinoxylan arabinofuranohydrolase isoenzyme
AXAH-II from GI:13398414 [Hordeum vulgare]
Length = 678
Score = 28.3 bits (60), Expect = 6.1
Identities = 16/69 (23%), Positives = 37/69 (53%)
Frame = +3
Query: 33 FRNKIAFFVPVRLINWKHSKVRHSLINFGLHTKQTRSRFSQVPVMGAFSPLDPSSFSRPE 212
++N ++ ++ +N+ + ++ GL R S+ V+ + + +D +SFS+PE
Sbjct: 566 WKNNGKDYIRIKAVNFGANSENMQVLVTGLDPNVMRVSGSKKTVLTSTNVMDENSFSQPE 625
Query: 213 QAVIKHVTL 239
+ V+ H +L
Sbjct: 626 K-VVPHESL 633
>At3g44670.1 68416.m04804 disease resistance protein
RPP1-Ws[A,C]-like (TIR-NBS-LRR class), putative domain
signature TIR-NBS-LRR exists, suggestive of a disease
resistance protein. Closest Col-0 homolog to both RPP1
Ws-A and RPP1 Ws-C
Length = 872
Score = 27.9 bits (59), Expect = 8.1
Identities = 12/33 (36%), Positives = 21/33 (63%)
Frame = +2
Query: 413 GSKLTIQLPKRASSGDKLKIKIKYTTSPSATAY 511
G+++ RA+SGD LKIK+K ++ P+ +
Sbjct: 664 GTQVPACFNHRATSGDSLKIKLKESSLPTTLRF 696
>At1g73960.1 68414.m08565 expressed protein similar to TATA binding
protein associated factor (GI:2827282) [Homo sapiens];
similar to Transcription initiation factor TFIID 150 kDa
subunit (TAFII-150) (TAFII150) (Swiss-Prot:Q24325)
[Drosophila melanogaster]
Length = 1390
Score = 27.9 bits (59), Expect = 8.1
Identities = 16/45 (35%), Positives = 25/45 (55%)
Frame = +2
Query: 254 FENKVLNGSATLDVDVLQDIGDVVLDSSELTIESIELDGAQLTYK 388
F+ + + G L+V V DIG V L + L IES+ +DG ++
Sbjct: 36 FKKRQIYGYTELEVSV-PDIGIVGLHAENLGIESVLVDGEPTVFE 79
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,597,253
Number of Sequences: 28952
Number of extensions: 301345
Number of successful extensions: 864
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 847
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 864
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1765546400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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