BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV30063 (477 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC74.05 |rpl2702|rpl27-2|60S ribosomal protein L27|Schizosacch... 116 1e-27 SPBC685.07c |rpl2701|rpl27-1|60S ribosomal protein L27|Schizosac... 112 3e-26 SPCC622.18 |rpl6||60S ribosomal protein L6|Schizosaccharomyces p... 31 0.069 SPAC1805.13 |rpl14||60S ribosomal protein L14|Schizosaccharomyce... 27 1.1 SPAC144.08 |||DNAJ domain protein Jac1 |Schizosaccharomyces pomb... 27 1.5 SPAC6F6.04c |||membrane transporter |Schizosaccharomyces pombe|c... 27 1.5 SPAC25G10.04c |rec10|rec20|meiotic recombination protein Rec10 |... 26 3.4 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 26 3.4 SPBC2F12.08c |ceg1|pce1|mRNA guanylyltransferase Ceg1|Schizosacc... 25 4.5 SPAC11D3.14c |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||... 25 5.9 SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr ... 25 5.9 SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom... 25 5.9 >SPCC74.05 |rpl2702|rpl27-2|60S ribosomal protein L27|Schizosaccharomyces pombe|chr 3|||Manual Length = 136 Score = 116 bits (280), Expect = 1e-27 Identities = 48/81 (59%), Positives = 65/81 (80%) Frame = +3 Query: 12 MGKIMKPGKVVLVLSGRYAGRKAIVVKNYDEGTSDKPYGHAFVAGIDRYPRKVHKRMGKN 191 M KI+KPGKV LV GR+AG+K ++++N D+G+ P+GHA VAG++RYP KV K MG Sbjct: 1 MVKILKPGKVALVTRGRFAGKKVVILQNVDQGSKSHPFGHAVVAGVERYPLKVTKSMGAK 60 Query: 192 KIHKRSKIKPFVKVVNYNHLM 254 +I KRS++KPF+KV+NYNHLM Sbjct: 61 RIAKRSRVKPFIKVINYNHLM 81 Score = 39.5 bits (88), Expect = 3e-04 Identities = 18/54 (33%), Positives = 30/54 (55%), Gaps = 2/54 (3%) Frame = +2 Query: 257 TRYTVDFSFEK--FSAKDLKDPAKRKKLRFNTRVRFEERYKSGKNKWFFQKLRF 412 TRY ++ K + +P++R + + FEE+Y++GK+ WFF LRF Sbjct: 83 TRYALELDNLKGLVTPTTFSEPSQRSAAKKTVKNTFEEKYQTGKSAWFFTPLRF 136 >SPBC685.07c |rpl2701|rpl27-1|60S ribosomal protein L27|Schizosaccharomyces pombe|chr 2|||Manual Length = 136 Score = 112 bits (269), Expect = 3e-26 Identities = 46/81 (56%), Positives = 64/81 (79%) Frame = +3 Query: 12 MGKIMKPGKVVLVLSGRYAGRKAIVVKNYDEGTSDKPYGHAFVAGIDRYPRKVHKRMGKN 191 M KI+KPGKV L+ GR+AG+K ++++ D+G+ P+GHA VAG++RYP KV K MG Sbjct: 1 MVKILKPGKVALITRGRFAGKKVVILQAIDQGSKSHPFGHAVVAGVERYPLKVTKSMGAK 60 Query: 192 KIHKRSKIKPFVKVVNYNHLM 254 +I +RS++KPF+KVVNYNHLM Sbjct: 61 RIARRSRVKPFIKVVNYNHLM 81 Score = 46.4 bits (105), Expect = 2e-06 Identities = 22/54 (40%), Positives = 31/54 (57%), Gaps = 2/54 (3%) Frame = +2 Query: 257 TRYTVDFSFEK--FSAKDLKDPAKRKKLRFNTRVRFEERYKSGKNKWFFQKLRF 412 TRY ++ K +A K+P +R R + FEE+Y+SGK+ WFF LRF Sbjct: 83 TRYALELDNLKGLITADTFKEPTQRSAARKTVKKTFEEKYQSGKSAWFFTPLRF 136 >SPCC622.18 |rpl6||60S ribosomal protein L6|Schizosaccharomyces pombe|chr 3|||Manual Length = 195 Score = 31.5 bits (68), Expect = 0.069 Identities = 10/34 (29%), Positives = 23/34 (67%) Frame = +3 Query: 3 PSKMGKIMKPGKVVLVLSGRYAGRKAIVVKNYDE 104 P+K+ + PG V ++L+GR+ G++ +V+ ++ Sbjct: 45 PTKLRASLAPGTVCILLAGRFRGKRVVVLSQLED 78 >SPAC1805.13 |rpl14||60S ribosomal protein L14|Schizosaccharomyces pombe|chr 1|||Manual Length = 134 Score = 27.5 bits (58), Expect = 1.1 Identities = 11/28 (39%), Positives = 18/28 (64%) Frame = +3 Query: 18 KIMKPGKVVLVLSGRYAGRKAIVVKNYD 101 + ++ G+VVLV G Y G+ A++V D Sbjct: 6 RYVEVGRVVLVTKGEYTGKLAVIVDIVD 33 >SPAC144.08 |||DNAJ domain protein Jac1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 225 Score = 27.1 bits (57), Expect = 1.5 Identities = 15/36 (41%), Positives = 17/36 (47%) Frame = +3 Query: 183 GKNKIHKRSKIKPFVKVVNYNHLMQHVIQLTSALKN 290 G + I R K P K+VNYNH H S KN Sbjct: 28 GNHWIFARFKFYPLNKIVNYNHF--HSSSCQSEAKN 61 >SPAC6F6.04c |||membrane transporter |Schizosaccharomyces pombe|chr 1|||Manual Length = 489 Score = 27.1 bits (57), Expect = 1.5 Identities = 17/73 (23%), Positives = 26/73 (35%) Frame = -1 Query: 411 NLSFWKNHLFFPLLYLSSKRTRVLKRSFLRFAGSFRSFALNFSKLKSTV*RVASSDYSLQ 232 N+ FW F P L+ + LKR G + + L + + S L Sbjct: 278 NVLFWVIQFFVPYLFTLILDAKALKRRTRGIIGLTIQAVVIMATLSGELGWIVSKHIDLH 337 Query: 231 PSRKALSWTSCGF 193 + L WT G+ Sbjct: 338 DTSPDLDWTQRGY 350 >SPAC25G10.04c |rec10|rec20|meiotic recombination protein Rec10 |Schizosaccharomyces pombe|chr 1|||Manual Length = 791 Score = 25.8 bits (54), Expect = 3.4 Identities = 11/30 (36%), Positives = 15/30 (50%) Frame = -3 Query: 118 LSEVPSS*FLTTIALRPAYRPLKTSTTLPG 29 L E+P + F T+ AY P S T+ G Sbjct: 689 LEEIPYNKFFKTVEKNEAYNPSSKSATIDG 718 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 25.8 bits (54), Expect = 3.4 Identities = 15/38 (39%), Positives = 19/38 (50%) Frame = -2 Query: 212 LGPLVDFILSHPLVHFPGVPVDTSDESMPVRLVGGTFV 99 LGP+ + + L HFP DT + S P GGT V Sbjct: 1364 LGPIGETFEVNGLSHFPSDIEDTIERSHPRIARGGTAV 1401 >SPBC2F12.08c |ceg1|pce1|mRNA guanylyltransferase Ceg1|Schizosaccharomyces pombe|chr 2|||Manual Length = 402 Score = 25.4 bits (53), Expect = 4.5 Identities = 14/45 (31%), Positives = 19/45 (42%) Frame = +2 Query: 275 FSFEKFSAKDLKDPAKRKKLRFNTRVRFEERYKSGKNKWFFQKLR 409 +SF F D K+ K K R E Y +N+W F + R Sbjct: 284 YSFFAFMYVDEKEWEKLKSFNVPLSERIVECYLDDENRWRFLRFR 328 >SPAC11D3.14c |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1260 Score = 25.0 bits (52), Expect = 5.9 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = +3 Query: 165 KVHKRMGKNKIHKRSKIKPFVKVV 236 K+ KR+G I SK+ P VK+V Sbjct: 205 KIAKRIGFTDISLSSKLTPMVKIV 228 >SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1337 Score = 25.0 bits (52), Expect = 5.9 Identities = 11/39 (28%), Positives = 19/39 (48%) Frame = +2 Query: 269 VDFSFEKFSAKDLKDPAKRKKLRFNTRVRFEERYKSGKN 385 + F K L DP+ R+K F ++ + +Y+ KN Sbjct: 150 LQFQIPSNEKKSLDDPSPRRKPSFFSKSNLKRKYRYLKN 188 >SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharomyces pombe|chr 2|||Manual Length = 1462 Score = 25.0 bits (52), Expect = 5.9 Identities = 10/33 (30%), Positives = 18/33 (54%) Frame = +2 Query: 269 VDFSFEKFSAKDLKDPAKRKKLRFNTRVRFEER 367 VD++ E+F DP+ + NT ++ E+R Sbjct: 821 VDYAIERFCQFTALDPSSVPGTQLNTAIKVEDR 853 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,837,674 Number of Sequences: 5004 Number of extensions: 35760 Number of successful extensions: 118 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 113 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 118 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 184476110 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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