BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV30049X (535 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 147 5e-36 At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 147 5e-36 At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 147 5e-36 At1g29760.1 68414.m03639 expressed protein 31 0.37 At4g10710.1 68417.m01751 transcriptional regulator-related simil... 31 0.48 At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ... 29 1.9 At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 27 5.9 At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 27 5.9 At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 27 5.9 At4g38440.1 68417.m05432 expressed protein 27 7.9 >At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal protein S4, Arabidopsis thaliana, PIR:T48480 Length = 262 Score = 147 bits (356), Expect = 5e-36 Identities = 66/84 (78%), Positives = 74/84 (88%) Frame = +3 Query: 3 KKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIV 182 KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ Sbjct: 6 KKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISIL 65 Query: 183 KQRLIKVDGKVRTDTTYPAGFMDV 254 QR I+VDGKVRTD TYPAGFMDV Sbjct: 66 MQRHIQVDGKVRTDKTYPAGFMDV 89 Score = 127 bits (307), Expect = 4e-30 Identities = 57/91 (62%), Positives = 70/91 (76%) Frame = +2 Query: 257 SIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYP 436 SI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRTIRYP Sbjct: 91 SIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTIRYP 150 Query: 437 DPLIKVNDSIQLDIATTKIMDFIKLSLGTCV 529 DPLIK ND+I+LD+ KI++FIK +G V Sbjct: 151 DPLIKPNDTIKLDLEANKIVEFIKFDVGNVV 181 >At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) Length = 262 Score = 147 bits (356), Expect = 5e-36 Identities = 66/84 (78%), Positives = 74/84 (88%) Frame = +3 Query: 3 KKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIV 182 KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ Sbjct: 6 KKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISIL 65 Query: 183 KQRLIKVDGKVRTDTTYPAGFMDV 254 QR I+VDGKVRTD TYPAGFMDV Sbjct: 66 MQRHIQVDGKVRTDKTYPAGFMDV 89 Score = 127 bits (306), Expect = 5e-30 Identities = 57/91 (62%), Positives = 70/91 (76%) Frame = +2 Query: 257 SIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYP 436 SI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRTIRYP Sbjct: 91 SIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTIRYP 150 Query: 437 DPLIKVNDSIQLDIATTKIMDFIKLSLGTCV 529 DPLIK ND+I+LD+ KI++FIK +G V Sbjct: 151 DPLIKPNDTIKLDLEENKIVEFIKFDVGNVV 181 >At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains ribosomal protein S4 signature from residues 8 to 22 Length = 261 Score = 147 bits (356), Expect = 5e-36 Identities = 66/84 (78%), Positives = 74/84 (88%) Frame = +3 Query: 3 KKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIV 182 KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ Sbjct: 6 KKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISIL 65 Query: 183 KQRLIKVDGKVRTDTTYPAGFMDV 254 QR I+VDGKVRTD TYPAGFMDV Sbjct: 66 MQRHIQVDGKVRTDKTYPAGFMDV 89 Score = 127 bits (306), Expect = 5e-30 Identities = 57/91 (62%), Positives = 70/91 (76%) Frame = +2 Query: 257 SIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYP 436 SI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRTIRYP Sbjct: 91 SIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTIRYP 150 Query: 437 DPLIKVNDSIQLDIATTKIMDFIKLSLGTCV 529 DPLIK ND+I+LD+ KI++FIK +G V Sbjct: 151 DPLIKPNDTIKLDLEENKIVEFIKFDVGNVV 181 >At1g29760.1 68414.m03639 expressed protein Length = 526 Score = 31.5 bits (68), Expect = 0.37 Identities = 14/33 (42%), Positives = 18/33 (54%) Frame = -1 Query: 229 VVSVLTFPSTFMRRCFTIFRTSFPVKAYFRRFL 131 + S+LTFP +R CF F F + RRFL Sbjct: 193 MTSLLTFPPWLLRNCFLFFFDPFSTIRFGRRFL 225 >At4g10710.1 68417.m01751 transcriptional regulator-related similar to chromatin-specific transcription elongation factor FACT 140 kDa subunit (GI:5499741) [Homo sapiens] Length = 1074 Score = 31.1 bits (67), Expect = 0.48 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = -1 Query: 112 KHSRNLWGPVDGLGAYTPPSLSNIHAL 32 KHS +LWG D L TPP+ ++ L Sbjct: 44 KHSADLWGSADALAIATPPASDDLRYL 70 >At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, putative / steroid 5-alpha-reductase, putative identical to gi:1280611; contains a steroid 5-alpha reductase, C-terminal domain Length = 262 Score = 29.1 bits (62), Expect = 1.9 Identities = 15/39 (38%), Positives = 19/39 (48%) Frame = -1 Query: 202 TFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGP 86 TF R C + P A +FL+ GKH+R WGP Sbjct: 8 TFFRYCLLTLIFAGPPTAVLLKFLQA-PYGKHNRTGWGP 45 >At4g08180.3 68417.m01353 oxysterol-binding family protein similar to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens}; contains Pfam profiles PF00169: PH domain, PF01237: Oxysterol-binding protein Length = 813 Score = 27.5 bits (58), Expect = 5.9 Identities = 12/33 (36%), Positives = 20/33 (60%) Frame = +3 Query: 135 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDTTY 233 N ++A+T NE+ +K+RL D ++R D Y Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 735 >At4g08180.2 68417.m01352 oxysterol-binding family protein similar to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens}; contains Pfam profiles PF00169: PH domain, PF01237: Oxysterol-binding protein Length = 813 Score = 27.5 bits (58), Expect = 5.9 Identities = 12/33 (36%), Positives = 20/33 (60%) Frame = +3 Query: 135 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDTTY 233 N ++A+T NE+ +K+RL D ++R D Y Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 735 >At4g08180.1 68417.m01351 oxysterol-binding family protein similar to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens}; contains Pfam profiles PF00169: PH domain, PF01237: Oxysterol-binding protein Length = 814 Score = 27.5 bits (58), Expect = 5.9 Identities = 12/33 (36%), Positives = 20/33 (60%) Frame = +3 Query: 135 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDTTY 233 N ++A+T NE+ +K+RL D ++R D Y Sbjct: 704 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 736 >At4g38440.1 68417.m05432 expressed protein Length = 1465 Score = 27.1 bits (57), Expect = 7.9 Identities = 11/33 (33%), Positives = 18/33 (54%) Frame = +3 Query: 15 KRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECL 113 +R+ P W L + V++ + STGP + E L Sbjct: 1140 QRMPLPPHWFLSAISAVHSGKTSTGPPESTELL 1172 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,630,430 Number of Sequences: 28952 Number of extensions: 267960 Number of successful extensions: 788 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 755 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 788 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 984125600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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