Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30046
(762 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 149 3e-37
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 149 3e-37
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 149 3e-37
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 82 1e-16
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 80 3e-16
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 51 2e-07
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 31 0.24
SPBC18H10.20c |||conserved fungal protein|Schizosaccharomyces po... 27 2.9
SPAC23D3.08 |usp108||U1 snRNP-associated protein Usp108|Schizosa... 27 3.9
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 3.9
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 6.7
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 26 6.7
SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|c... 25 8.9
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 8.9
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 25 8.9
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 149 bits (362), Expect = 3e-37
Identities = 67/98 (68%), Positives = 77/98 (78%)
Frame = +3
Query: 228 PSCCRFRAHSGWHGDNMLEPSTKMPWFKAWQVERKEGKADGKCLIEALDAILPPARPTDK 407
P F SG+ GDNM+EP+T MPW++ WQ E K G GK L+EA+D+I PPARPTDK
Sbjct: 183 PKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDK 242
Query: 408 PLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVSLPP 521
PLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV+ P
Sbjct: 243 PLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAP 280
Score = 145 bits (352), Expect = 5e-36
Identities = 65/83 (78%), Positives = 73/83 (87%)
Frame = +2
Query: 512 FAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAAD 691
FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A
Sbjct: 278 FAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCAS 337
Query: 692 FTAQVIVLNHPGQISNGYTPVLD 760
FTAQVI+LNHPGQIS GY+PVLD
Sbjct: 338 FTAQVIILNHPGQISAGYSPVLD 360
Score = 130 bits (314), Expect = 2e-31
Identities = 61/85 (71%), Positives = 73/85 (85%)
Frame = +1
Query: 1 ADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFE 180
ADCA+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFE
Sbjct: 109 ADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFE 166
Query: 181 EIKKEVSSYIKKIGYNPAAVAFVPI 255
EI KE S++IKK+G+NP V FVP+
Sbjct: 167 EIVKETSNFIKKVGFNPKTVPFVPV 191
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 149 bits (362), Expect = 3e-37
Identities = 67/98 (68%), Positives = 77/98 (78%)
Frame = +3
Query: 228 PSCCRFRAHSGWHGDNMLEPSTKMPWFKAWQVERKEGKADGKCLIEALDAILPPARPTDK 407
P F SG+ GDNM+EP+T MPW++ WQ E K G GK L+EA+D+I PPARPTDK
Sbjct: 183 PKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDK 242
Query: 408 PLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVSLPP 521
PLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV+ P
Sbjct: 243 PLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAP 280
Score = 145 bits (352), Expect = 5e-36
Identities = 65/83 (78%), Positives = 73/83 (87%)
Frame = +2
Query: 512 FAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAAD 691
FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A
Sbjct: 278 FAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCAS 337
Query: 692 FTAQVIVLNHPGQISNGYTPVLD 760
FTAQVI+LNHPGQIS GY+PVLD
Sbjct: 338 FTAQVIILNHPGQISAGYSPVLD 360
Score = 130 bits (315), Expect = 2e-31
Identities = 62/85 (72%), Positives = 73/85 (85%)
Frame = +1
Query: 1 ADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFE 180
ADCAVLI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFE
Sbjct: 109 ADCAVLIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFE 166
Query: 181 EIKKEVSSYIKKIGYNPAAVAFVPI 255
EI KE S++IKK+G+NP V FVP+
Sbjct: 167 EIVKETSNFIKKVGFNPKTVPFVPV 191
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 149 bits (362), Expect = 3e-37
Identities = 67/98 (68%), Positives = 77/98 (78%)
Frame = +3
Query: 228 PSCCRFRAHSGWHGDNMLEPSTKMPWFKAWQVERKEGKADGKCLIEALDAILPPARPTDK 407
P F SG+ GDNM+EP+T MPW++ WQ E K G GK L+EA+D+I PPARPTDK
Sbjct: 183 PKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDK 242
Query: 408 PLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVSLPP 521
PLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV+ P
Sbjct: 243 PLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAP 280
Score = 145 bits (352), Expect = 5e-36
Identities = 65/83 (78%), Positives = 73/83 (87%)
Frame = +2
Query: 512 FAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAAD 691
FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A
Sbjct: 278 FAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCAS 337
Query: 692 FTAQVIVLNHPGQISNGYTPVLD 760
FTAQVI+LNHPGQIS GY+PVLD
Sbjct: 338 FTAQVIILNHPGQISAGYSPVLD 360
Score = 130 bits (314), Expect = 2e-31
Identities = 61/85 (71%), Positives = 73/85 (85%)
Frame = +1
Query: 1 ADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFE 180
ADCA+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFE
Sbjct: 109 ADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFE 166
Query: 181 EIKKEVSSYIKKIGYNPAAVAFVPI 255
EI KE S++IKK+G+NP V FVP+
Sbjct: 167 EIVKETSNFIKKVGFNPKTVPFVPV 191
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 81.8 bits (193), Expect = 1e-16
Identities = 38/87 (43%), Positives = 59/87 (67%), Gaps = 2/87 (2%)
Frame = +1
Query: 1 ADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFE 180
AD VL+++A GEFEAG + GQTREHA+LA T G+ L+V +NKMD +SE R++
Sbjct: 340 ADIGVLVISARRGEFEAGFERGGQTREHAVLARTQGINHLVVVINKMDEPSVQWSEERYK 399
Query: 181 EIKKEVSSYIKKI-GYNPAA-VAFVPI 255
E ++S +++++ GYN V ++P+
Sbjct: 400 ECVDKLSMFLRRVAGYNSKTDVKYMPV 426
Score = 39.5 bits (88), Expect = 5e-04
Identities = 24/81 (29%), Positives = 41/81 (50%), Gaps = 1/81 (1%)
Frame = +2
Query: 518 PANITTEVKSV-EMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADF 694
P N T EV ++ + E + ++ GD V V+ +++ GYV +KN P F
Sbjct: 503 PINQTLEVTAIYDEADEEISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRF 560
Query: 695 TAQVIVLNHPGQISNGYTPVL 757
AQ+ +L P ++ GY+ V+
Sbjct: 561 IAQIAILELPSILTTGYSCVM 581
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 80.2 bits (189), Expect = 3e-16
Identities = 42/86 (48%), Positives = 56/86 (65%), Gaps = 1/86 (1%)
Frame = +1
Query: 1 ADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFE 180
AD AVL+V + FE G +NGQTREHA L LG+ +++V VNK+D +SE RF+
Sbjct: 279 ADFAVLVVDSSQNNFERGFLENGQTREHAYLLRALGISEIVVSVNKLDLMS--WSEDRFQ 336
Query: 181 EIKKEVSSY-IKKIGYNPAAVAFVPI 255
EIK VS + IK +G+ + V FVPI
Sbjct: 337 EIKNIVSDFLIKMVGFKTSNVHFVPI 362
Score = 45.2 bits (102), Expect = 1e-05
Identities = 23/46 (50%), Positives = 29/46 (63%)
Frame = +3
Query: 348 GKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETG 485
G L+ ALD ++PP +P KPLRL + DVY+ TV GRVE G
Sbjct: 384 GPTLLSALDQLVPPEKPYRKPLRLSIDDVYRSPRSVTV-TGRVEAG 428
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 51.2 bits (117), Expect = 2e-07
Identities = 27/65 (41%), Positives = 39/65 (60%), Gaps = 1/65 (1%)
Frame = +3
Query: 357 LIEALDAILP-PARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVSLPPPTSL 533
L+EA+D+ + P R TD P + ++DV+ I G GTV GRVE G LK G + + S
Sbjct: 234 LMEAVDSYITLPERKTDVPFLMAIEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSH 293
Query: 534 LKSNL 548
LK+ +
Sbjct: 294 LKTTV 298
Score = 47.2 bits (107), Expect = 3e-06
Identities = 27/68 (39%), Positives = 41/68 (60%)
Frame = +1
Query: 4 DCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEE 183
D A+++V+A G+ QTREH LLA +GVKQ++V +NK+D EP E E
Sbjct: 141 DGAIIVVSATDGQMP-------QTREHLLLARQVGVKQIVVYINKVDMVEPDMIELVEME 193
Query: 184 IKKEVSSY 207
+++ +S Y
Sbjct: 194 MRELLSEY 201
Score = 35.1 bits (77), Expect = 0.011
Identities = 14/43 (32%), Positives = 27/43 (62%)
Frame = +2
Query: 521 ANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYV 649
+++ T V +EM + L AV GDN G ++++ ++L+RG +
Sbjct: 292 SHLKTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMI 334
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 30.7 bits (66), Expect = 0.24
Identities = 21/68 (30%), Positives = 30/68 (44%)
Frame = +3
Query: 297 MPWFKAWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRV 476
+P K V K GK + L E + I PP + PLR L D + G + + R+
Sbjct: 206 IPMSKPILVSSKTGKNVEQILPEIIQKIPPPKGSENAPLRCLLIDSWYNSYQGVIGLVRI 265
Query: 477 ETGVLKPG 500
G +K G
Sbjct: 266 MEGFIKKG 273
>SPBC18H10.20c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 361
Score = 27.1 bits (57), Expect = 2.9
Identities = 13/37 (35%), Positives = 17/37 (45%)
Frame = -1
Query: 597 TLSPGTASWRASWCISTDLTSVVMLAGAKTQWYLVST 487
T PGT W SW L + V K ++YL +T
Sbjct: 101 TYRPGTQHWPFSWLFPGSLPASVSNRYIKLEYYLEAT 137
>SPAC23D3.08 |usp108||U1 snRNP-associated protein
Usp108|Schizosaccharomyces pombe|chr 1|||Manual
Length = 382
Score = 26.6 bits (56), Expect = 3.9
Identities = 23/76 (30%), Positives = 32/76 (42%), Gaps = 3/76 (3%)
Frame = -3
Query: 637 TQFLDGHVLYVET---YIVSRYSFLESFVVHLHRFDFSSDVGGGKDTMVPGFNTPVSTLP 467
T +D V+ +ET YI+SR SF+ V + D + + G T + P
Sbjct: 162 TNEIDASVV-IETLLKYILSRISFIPRVSVVPYIPDAAYGIYGSPSTFTQFNHMSSMVTP 220
Query: 466 TGTVPIPPILYTSCRG 419
IPPI TS G
Sbjct: 221 ENPYGIPPIALTSAGG 236
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 3.9
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -3
Query: 634 QFLDGHVLYVETYIVSRYSFLESFV 560
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 25.8 bits (54), Expect = 6.7
Identities = 10/39 (25%), Positives = 22/39 (56%)
Frame = -3
Query: 658 VTSNITTTQFLDGHVLYVETYIVSRYSFLESFVVHLHRF 542
+T ++ + L H+ +++ SR +FL ++H+H F
Sbjct: 1012 LTQSLQSFSHLSNHIEVLDSTRQSRLTFLCHLILHMHGF 1050
>SPBC1539.08 |||ADP-ribosylation factor, Arf
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 25.8 bits (54), Expect = 6.7
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = +1
Query: 226 NPAAVAFVPILDGTETTCWSLQPKCLGSRHGRWSVRKAKLTENASLK 366
+PA + V LD + W++QP C + G A L++NA LK
Sbjct: 139 SPAQITDVLQLDKLKDRLWNVQPTCALTGDGLLE-GLAWLSQNAKLK 184
>SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 367
Score = 25.4 bits (53), Expect = 8.9
Identities = 9/30 (30%), Positives = 18/30 (60%)
Frame = +2
Query: 161 TVSPDLRKSRRKYPHTSRRLATTQLLSLSC 250
+ +PD+ ++PHT +R +T + L + C
Sbjct: 145 STNPDVLYMSSQHPHTQQRYSTLKRLMVRC 174
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 8.9
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -3
Query: 490 NTPVSTLPTGTVPIPPIL 437
N P S LPT VP+P I+
Sbjct: 666 NPPTSALPTVPVPLPSII 683
>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 25.4 bits (53), Expect = 8.9
Identities = 20/60 (33%), Positives = 23/60 (38%), Gaps = 5/60 (8%)
Frame = -3
Query: 499 PGFNTPVSTLPTGTVPIP-PILYTSCR--GRRRGLSVGRAGGRMASRASMRH--FPSALP 335
P +N P G+VP+P P S R R S GR S H PS LP
Sbjct: 314 PNYNAPFQPAGVGSVPLPAPTSSQSLRLGSLHRSRSPSPRSGRPRRSPSPSHLSIPSTLP 373
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,551,978
Number of Sequences: 5004
Number of extensions: 81409
Number of successful extensions: 275
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 249
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 270
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 365309308
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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