BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV30035X (555 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces pomb... 31 0.086 SPCC895.07 |alp14|mtc1|Mad2-dependent spindle checkpoint compone... 31 0.15 SPAC23C11.03 |||U3 snoRNP-associated protein Mpp1 |Schizosacchar... 27 2.5 SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit ... 27 2.5 SPAC27F1.02c |cdc8|fus4|tropomyosin|Schizosaccharomyces pombe|ch... 26 3.2 SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces... 25 5.7 SPBC83.16c |||conserved fungal protein|Schizosaccharomyces pombe... 25 5.7 SPAC144.14 |klp8||kinesin-like protein Klp8|Schizosaccharomyces ... 25 5.7 SPAC458.03 |||nuclear telomere cap complex subunit |Schizosaccha... 25 7.5 SPAC1A6.09c |lag1||sphingosine N-acyltransferase Lag1|Schizosacc... 25 9.9 >SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 952 Score = 31.5 bits (68), Expect = 0.086 Identities = 14/63 (22%), Positives = 34/63 (53%) Frame = -3 Query: 532 YNRRV*IIESTLDELNSALDFLERKNDDIHQQLKELLQSNIAIRQEMREENKDVSNSN*R 353 Y ++ I +++++E+ + L+ + + + Q+K LL+ N ++++ + D N Sbjct: 306 YEEKLSIAQNSINEIQTQNRDLKLETEKLQDQIKALLERNQSLQEALETVKNDEKNLREM 365 Query: 352 NAN 344 NAN Sbjct: 366 NAN 368 Score = 25.4 bits (53), Expect = 5.7 Identities = 12/44 (27%), Positives = 26/44 (59%) Frame = -3 Query: 490 LNSALDFLERKNDDIHQQLKELLQSNIAIRQEMREENKDVSNSN 359 L S + E + +H L+ L Q+N +++ E+ E+N ++++ N Sbjct: 520 LKSQIRDQELTIEKLHDSLETLSQTNNSLQCEISEKNAELNSVN 563 >SPCC895.07 |alp14|mtc1|Mad2-dependent spindle checkpoint component |Schizosaccharomyces pombe|chr 3|||Manual Length = 809 Score = 30.7 bits (66), Expect = 0.15 Identities = 12/37 (32%), Positives = 27/37 (72%) Frame = -3 Query: 496 DELNSALDFLERKNDDIHQQLKELLQSNIAIRQEMRE 386 D S+L+ L+R+N+++ +QLK + NI++++++ E Sbjct: 646 DSTISSLESLKRENEELREQLKVEHEENISMQKQLSE 682 >SPAC23C11.03 |||U3 snoRNP-associated protein Mpp1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 598 Score = 26.6 bits (56), Expect = 2.5 Identities = 12/36 (33%), Positives = 21/36 (58%) Frame = -3 Query: 466 ERKNDDIHQQLKELLQSNIAIRQEMREENKDVSNSN 359 +R+N Q +++L +SN+ + + E K SNSN Sbjct: 550 KRRNSGTEQVVRQLSKSNVEVIGKGGERKKVASNSN 585 >SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit Rpt5|Schizosaccharomyces pombe|chr 1|||Manual Length = 438 Score = 26.6 bits (56), Expect = 2.5 Identities = 12/51 (23%), Positives = 26/51 (50%) Frame = -3 Query: 514 IIESTLDELNSALDFLERKNDDIHQQLKELLQSNIAIRQEMREENKDVSNS 362 I+ + +DELNS LE + + + L + ++++E + +SN+ Sbjct: 31 ILAAGIDELNSRTRLLENDIKVMKSEFQRLTHEKSTMLEKIKENQEKISNN 81 >SPAC27F1.02c |cdc8|fus4|tropomyosin|Schizosaccharomyces pombe|chr 1|||Manual Length = 161 Score = 26.2 bits (55), Expect = 3.2 Identities = 10/28 (35%), Positives = 18/28 (64%) Frame = -3 Query: 469 LERKNDDIHQQLKELLQSNIAIRQEMRE 386 LER+ DD+ Q+L+E+ ++ E+ E Sbjct: 126 LERERDDMEQKLEEMTDKYTKVKAELDE 153 >SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces pombe|chr 3|||Manual Length = 1526 Score = 25.4 bits (53), Expect = 5.7 Identities = 13/25 (52%), Positives = 18/25 (72%) Frame = -3 Query: 502 TLDELNSALDFLERKNDDIHQQLKE 428 T ELNSALD +++ +IH +LKE Sbjct: 1349 TRKELNSALDSCKKREAEIH-RLKE 1372 >SPBC83.16c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 563 Score = 25.4 bits (53), Expect = 5.7 Identities = 11/50 (22%), Positives = 26/50 (52%) Frame = -1 Query: 465 SVKMMIFTNN*KSFCNPILQLDKKCVKKIRTSATVIEEMQIQHYFKFNCA 316 S+ +M+ + FC+ + K V K+R + ++ ++ +H+ F+ A Sbjct: 103 SIGLMLLLSALIGFCSESIVTSVKSVYKLRKAHSIFSKINKRHFDHFSAA 152 >SPAC144.14 |klp8||kinesin-like protein Klp8|Schizosaccharomyces pombe|chr 1|||Manual Length = 511 Score = 25.4 bits (53), Expect = 5.7 Identities = 14/42 (33%), Positives = 25/42 (59%) Frame = -3 Query: 490 LNSALDFLERKNDDIHQQLKELLQSNIAIRQEMREENKDVSN 365 +++ L+ LE DD + +LKE NIA +QE+ ++S+ Sbjct: 433 VDAYLNPLEFNFDDKNVKLKEFGSPNIAYKQELNSFQGELSS 474 >SPAC458.03 |||nuclear telomere cap complex subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 868 Score = 25.0 bits (52), Expect = 7.5 Identities = 13/38 (34%), Positives = 25/38 (65%), Gaps = 3/38 (7%) Frame = -1 Query: 453 MIFTNN*KSFCNPILQLDKK-CV--KKIRTSATVIEEM 349 ++ ++N K+F NP++ L +K CV +K+ TV ++M Sbjct: 202 LLHSSNLKAFINPLIPLTQKFCVQLQKLFADLTVSDQM 239 >SPAC1A6.09c |lag1||sphingosine N-acyltransferase Lag1|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 24.6 bits (51), Expect = 9.9 Identities = 11/53 (20%), Positives = 27/53 (50%), Gaps = 2/53 (3%) Frame = -3 Query: 247 YHLSIFSDQYHKVSVSF--K*CIIIHLQYLSMNNYLACLHHLNVISCIFMSVG 95 Y++S Y+ + + F + +++HL+ +++ HH+ + I +S G Sbjct: 195 YYMSPLFKAYYLIQLGFWLQQILVLHLEQRRADHWQMFAHHIVTCALIILSYG 247 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,981,705 Number of Sequences: 5004 Number of extensions: 37801 Number of successful extensions: 102 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 97 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 102 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 231978230 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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