Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30021
(374 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g34555.1 68417.m04910 40S ribosomal protein S25, putative 70 4e-13
At4g39200.1 68417.m05550 40S ribosomal protein S25 (RPS25E) ribo... 69 1e-12
At2g21580.1 68415.m02567 40S ribosomal protein S25 (RPS25B) 69 1e-12
At2g16360.1 68415.m01872 40S ribosomal protein S25 (RPS25A) 67 3e-12
At5g64950.1 68418.m08170 mitochondrial transcription termination... 29 0.76
At1g14500.1 68414.m01719 ankyrin repeat family protein contains ... 29 1.3
At2g17250.1 68415.m01992 expressed protein weak similarity to Ri... 28 2.3
At3g60980.1 68416.m06824 pentatricopeptide (PPR) repeat-containi... 27 4.1
At2g23170.1 68415.m02768 auxin-responsive GH3 family protein sim... 27 5.4
At1g24706.1 68414.m03104 expressed protein 27 5.4
At5g54770.1 68418.m06822 thiazole biosynthetic enzyme, chloropla... 26 7.1
At1g48180.1 68414.m05378 expressed protein ; expression supporte... 26 7.1
At5g44000.1 68418.m05384 glutathione S-transferase C-terminal do... 26 9.4
At5g38830.1 68418.m04697 tRNA synthetase class I (C) family prot... 26 9.4
At5g27640.1 68418.m03311 eukaryotic translation initiation facto... 26 9.4
At4g36670.1 68417.m05203 mannitol transporter, putative similar ... 26 9.4
At2g02810.1 68415.m00226 UDP-galactose/UDP-glucose transporter c... 26 9.4
>At4g34555.1 68417.m04910 40S ribosomal protein S25, putative
Length = 108
Score = 70.1 bits (164), Expect = 4e-13
Identities = 30/51 (58%), Positives = 42/51 (82%)
Frame = +1
Query: 109 LNNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKVRGSLARRALIELRKK 261
+NN VLFD+ TY+KL E P++KLITP+++S+RL++ GSLARRA+ EL K
Sbjct: 37 VNNMVLFDQATYDKLLSEAPKFKLITPSILSDRLRINGSLARRAIRELMAK 87
Score = 29.1 bits (62), Expect = 1.0
Identities = 13/22 (59%), Positives = 14/22 (63%)
Frame = +3
Query: 258 KGLIKQVVQHHGQVIYTRATKG 323
KG I+ V H Q IYTRAT G
Sbjct: 87 KGTIRMVSAHSSQQIYTRATHG 108
>At4g39200.1 68417.m05550 40S ribosomal protein S25 (RPS25E)
ribosomal protein S25, Lycopersicon esculentum,
PIR2:S40089
Length = 108
Score = 68.9 bits (161), Expect = 1e-12
Identities = 29/51 (56%), Positives = 42/51 (82%)
Frame = +1
Query: 109 LNNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKVRGSLARRALIELRKK 261
+NN VLFD+ TY+KL E P++KLITP+++S+R+++ GSLARRA+ EL K
Sbjct: 37 VNNMVLFDQATYDKLLTEAPKFKLITPSILSDRMRINGSLARRAIRELMAK 87
Score = 28.7 bits (61), Expect = 1.3
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = +3
Query: 258 KGLIKQVVQHHGQVIYTRAT 317
KG+I+ V H Q IYTRAT
Sbjct: 87 KGVIRMVAAHSSQQIYTRAT 106
>At2g21580.1 68415.m02567 40S ribosomal protein S25 (RPS25B)
Length = 108
Score = 68.5 bits (160), Expect = 1e-12
Identities = 29/51 (56%), Positives = 42/51 (82%)
Frame = +1
Query: 109 LNNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKVRGSLARRALIELRKK 261
+NN VLFD+ TY+KL E P++KLITP+++S+R+++ GSLARRA+ EL K
Sbjct: 37 VNNMVLFDQGTYDKLLTEAPKFKLITPSILSDRMRINGSLARRAIRELMAK 87
Score = 29.1 bits (62), Expect = 1.0
Identities = 13/20 (65%), Positives = 14/20 (70%)
Frame = +3
Query: 258 KGLIKQVVQHHGQVIYTRAT 317
KGLI+ V H Q IYTRAT
Sbjct: 87 KGLIRMVSAHSSQQIYTRAT 106
>At2g16360.1 68415.m01872 40S ribosomal protein S25 (RPS25A)
Length = 125
Score = 67.3 bits (157), Expect = 3e-12
Identities = 28/51 (54%), Positives = 42/51 (82%)
Frame = +1
Query: 109 LNNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKVRGSLARRALIELRKK 261
+NN VLFD+ TY+KL E P++KLITP+++S+RL++ GSLAR+A+ +L K
Sbjct: 53 VNNMVLFDQATYDKLMSEAPKFKLITPSILSDRLRINGSLARKAIRDLMVK 103
>At5g64950.1 68418.m08170 mitochondrial transcription termination
factor-related / mTERF-related contains Pfam profile
PF02536: mTERF
Length = 391
Score = 29.5 bits (63), Expect = 0.76
Identities = 17/49 (34%), Positives = 26/49 (53%)
Frame = -1
Query: 344 SLCDWIIALGRTRVDHLPMMLDYLFDETFFLSSMSALLAREPRTFNLSD 198
S C W++ + LP + YL S +++LL R+PR FNLS+
Sbjct: 166 SRCGWLLLSRDPNLFLLPN-ISYLETCGIVGSQLASLLRRQPRIFNLSE 213
>At1g14500.1 68414.m01719 ankyrin repeat family protein contains
Pfam domain, PF00023: Ankyrin repeat
Length = 436
Score = 28.7 bits (61), Expect = 1.3
Identities = 12/49 (24%), Positives = 24/49 (48%), Gaps = 2/49 (4%)
Frame = -1
Query: 173 YCGTSLYSFSYVGLSN--NTWLFNLSRTFPLDHFFFLALPPPDPSFFFC 33
+C LY+F + + + TW F + + + + +A+ P+P F C
Sbjct: 342 FCCALLYTFCLLPIGSLFTTWFFWIGASLGVSYALAMAIISPNPLLFLC 390
>At2g17250.1 68415.m01992 expressed protein weak similarity to
Ribosome biogenesis protein MAK21 (Swiss-Prot:Q12176)
[Saccharomyces cerevisiae]
Length = 577
Score = 27.9 bits (59), Expect = 2.3
Identities = 13/47 (27%), Positives = 29/47 (61%)
Frame = +1
Query: 112 NNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKVRGSLARRALIEL 252
+++ + +KPT +K E L++PA +S+R+K++ + A + + L
Sbjct: 245 SDESISEKPTDKKKKTEKGDSTLLSPATISKRMKLKFTKAWISFLRL 291
>At3g60980.1 68416.m06824 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 412
Score = 27.1 bits (57), Expect = 4.1
Identities = 20/69 (28%), Positives = 30/69 (43%), Gaps = 1/69 (1%)
Frame = -1
Query: 353 LYHSLCDWI-IALGRTRVDHLPMMLDYLFDETFFLSSMSALLAREPRTFNLSDTTAGVIS 177
LYH L D G D + +M+D FD +F +M P+ LSD +I+
Sbjct: 289 LYHELLDKNGTGKGCLDSDTIKIMVDECFDMGWFSEAMETYKKARPKNDYLSDKY--IIT 346
Query: 176 LYCGTSLYS 150
+C + S
Sbjct: 347 RFCENRMLS 355
>At2g23170.1 68415.m02768 auxin-responsive GH3 family protein
similar to auxin-responsive GH3 product [Glycine max]
GI:18591; contains Pfam profile PF03321: GH3
auxin-responsive promoter
Length = 595
Score = 26.6 bits (56), Expect = 5.4
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = -3
Query: 147 LICGFIKQHLVVQLVTNFSFGPLLLLGFAAT 55
++CG + +H V++L F+ G L +GF T
Sbjct: 213 MLCGLLMRHEVLRLGAVFASGLLRAIGFLQT 243
>At1g24706.1 68414.m03104 expressed protein
Length = 1781
Score = 26.6 bits (56), Expect = 5.4
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = -1
Query: 110 NLSRTFPLDHFFFLALPPPDP 48
+L ++ P DHF LPPP P
Sbjct: 1562 SLEKSHPDDHFHSQGLPPPPP 1582
>At5g54770.1 68418.m06822 thiazole biosynthetic enzyme, chloroplast
(ARA6) (THI1) (THI4) identical to SP|Q38814 Thiazole
biosynthetic enzyme, chloroplast precursor (ARA6)
{Arabidopsis thaliana}
Length = 349
Score = 26.2 bits (55), Expect = 7.1
Identities = 14/33 (42%), Positives = 22/33 (66%), Gaps = 3/33 (9%)
Frame = -1
Query: 275 LFDETFFLSSMSAL---LAREPRTFNLSDTTAG 186
LFD +F S++SA + +PR+F++ TTAG
Sbjct: 18 LFDSSFHGSAISAAPISIGLKPRSFSVRATTAG 50
>At1g48180.1 68414.m05378 expressed protein ; expression supported
by MPSS
Length = 239
Score = 26.2 bits (55), Expect = 7.1
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = -1
Query: 206 LSDTTAGVISLYCGTSLYSFSYVGL 132
L + + +YCGTS SYVGL
Sbjct: 149 LQQDASAITGIYCGTSGEPASYVGL 173
>At5g44000.1 68418.m05384 glutathione S-transferase C-terminal
domain-containing protein contains Pfam domain PF00043:
Glutathione S-transferase, C-terminal domain
Length = 399
Score = 25.8 bits (54), Expect = 9.4
Identities = 13/44 (29%), Positives = 22/44 (50%), Gaps = 3/44 (6%)
Frame = -3
Query: 261 LFPKLDECSSRQGTSDL*SFR---YDSRCD*LVLWDFLVQFLIC 139
L P LD+ + + ++ R YD RC +LWD + ++C
Sbjct: 161 LIPSLDKANRCRNLKEVYKSRSGGYDGRCTVPMLWDLRKKDVVC 204
>At5g38830.1 68418.m04697 tRNA synthetase class I (C) family protein
similar to SP|Q06752 Cysteinyl-tRNA synthetase (EC
6.1.1.16) (Cysteine--tRNA ligase) (CysRS) {Bacillus
subtilis}; contains Pfam profile PF01406: tRNA
synthetases class I (C)
Length = 511
Score = 25.8 bits (54), Expect = 9.4
Identities = 11/21 (52%), Positives = 14/21 (66%), Gaps = 1/21 (4%)
Frame = -1
Query: 194 TAGVISLY-CGTSLYSFSYVG 135
T G I LY CG + Y FS++G
Sbjct: 25 TPGKIGLYVCGITAYDFSHIG 45
>At5g27640.1 68418.m03311 eukaryotic translation initiation factor 3
subunit 9 / eIF-3 eta / eIF3b (TIF3B1) nearly identical
to SP|Q9C5Z1 Eukaryotic translation initiation factor 3
subunit 9 (eIF-3 eta) (eIF3 p110) (eIF3b) {Arabidopsis
thaliana}
Length = 712
Score = 25.8 bits (54), Expect = 9.4
Identities = 16/46 (34%), Positives = 21/46 (45%), Gaps = 2/46 (4%)
Frame = -1
Query: 173 YCGTSLYSFSYVGLSNNTWLFNLSRTFPL--DHFFFLALPPPDPSF 42
Y T++ S + W FN + + DHFF LA P PSF
Sbjct: 566 YVATAVTSVHEMENGFTIWSFNGIMLYRILKDHFFQLAWRPRPPSF 611
>At4g36670.1 68417.m05203 mannitol transporter, putative similar to
mannitol transporter [Apium graveolens var. dulce]
GI:12004316; contains Pfam profile PF00083: major
facilitator superfamily protein
Length = 493
Score = 25.8 bits (54), Expect = 9.4
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = -1
Query: 185 VISLYCGTSLYSFSYVGLSNNTWLFNLSRTFPL 87
V+S+ S +F +GL TW+++ S FPL
Sbjct: 377 VLSIVAAYSFVAFFSIGLGPITWVYS-SEVFPL 408
>At2g02810.1 68415.m00226 UDP-galactose/UDP-glucose transporter
contains transmembrane domains; identical to
UDP-galactose/UDP-glucose transporter (GI:22651763)
[Arabidopsis thaliana] similar to UGTrel1 (GI:1669564)
[Rattus rattus]; identical to cDNA
UDP-galactose/UDP-glucose transporter GI:22651762
Length = 332
Score = 25.8 bits (54), Expect = 9.4
Identities = 9/25 (36%), Positives = 16/25 (64%)
Frame = -3
Query: 168 WDFLVQFLICGFIKQHLVVQLVTNF 94
WD L ++ ICG + Q+ + ++NF
Sbjct: 245 WDIL-KYCICGAVGQNFIFMTISNF 268
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,379,125
Number of Sequences: 28952
Number of extensions: 171885
Number of successful extensions: 545
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 533
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 545
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 507810264
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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