Bombyx BLAST Result against nematostella

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV30020
         (588 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_43840| Best HMM Match : NDK (HMM E-Value=0)                        123   9e-29
SB_45281| Best HMM Match : No HMM Matches (HMM E-Value=.)              46   3e-05
SB_13421| Best HMM Match : Harpin (HMM E-Value=0.62)                   28   4.9  
SB_56833| Best HMM Match : No HMM Matches (HMM E-Value=.)              27   8.6  

>SB_43840| Best HMM Match : NDK (HMM E-Value=0)
          Length = 786

 Score =  123 bits (297), Expect = 9e-29
 Identities = 54/77 (70%), Positives = 61/77 (79%)
 Frame = +3

Query: 258 MVWEGLNVVKTGRQMLGATNPADSQPGTIRGDLCIQVGRNIIHGSDSVESAKKEIGLWFT 437
           MVWEGL VVKT R MLG T+PA S PGTIRGD  I +GRNIIHGSD+VE+AK+EI LWF 
Sbjct: 710 MVWEGLGVVKTARVMLGETDPAKSLPGTIRGDFSIHIGRNIIHGSDAVETAKEEIALWFK 769

Query: 438 DKEVVGWTPANENWVYE 488
           D E+V WTP N  W+YE
Sbjct: 770 DDELVDWTPCNNPWMYE 786



 Score =  116 bits (280), Expect = 1e-26
 Identities = 55/70 (78%), Positives = 62/70 (88%)
 Frame = +1

Query: 46  ERTFIMVKPDGVQRGLVGTIIERFEKKGFKLVGLKFVWPSEELLQQHYSDLASRPFFPGL 225
           ERTFIMVKPDGVQRGLVG II+RFE+KGFKLV LK V  SEE L++HY+DLA  PF+PGL
Sbjct: 127 ERTFIMVKPDGVQRGLVGEIIKRFEQKGFKLVALKMVQESEEHLKKHYADLAHLPFYPGL 186

Query: 226 VKYMSSGPVV 255
           VK+MSSGPVV
Sbjct: 187 VKFMSSGPVV 196



 Score = 96.3 bits (229), Expect = 2e-20
 Identities = 45/76 (59%), Positives = 55/76 (72%), Gaps = 2/76 (2%)
 Frame = +1

Query: 46  ERTFIMVKPDGVQRGLVGTIIERFEKKGFKLVGLKFVWPSEELLQQHYSDLASRPFFPGL 225
           ERTF+M+KPD V RGL+G II RFEKKGFKLV +KFV  SE+  ++HY  LA   F+ GL
Sbjct: 639 ERTFLMIKPDAVSRGLIGEIISRFEKKGFKLVAMKFVKKSEDHFRKHYESLAKLKFYDGL 698

Query: 226 VKYMSSGPV--VLWYG 267
            KYMS  PV  ++W G
Sbjct: 699 CKYMSQTPVCAMVWEG 714



 Score = 34.3 bits (75), Expect = 0.075
 Identities = 12/17 (70%), Positives = 15/17 (88%)
 Frame = +3

Query: 321 ADSQPGTIRGDLCIQVG 371
           ADS+PGTIRGD C+ +G
Sbjct: 199 ADSKPGTIRGDFCVHIG 215


>SB_45281| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1256

 Score = 45.6 bits (103), Expect = 3e-05
 Identities = 26/70 (37%), Positives = 39/70 (55%)
 Frame = +1

Query: 46  ERTFIMVKPDGVQRGLVGTIIERFEKKGFKLVGLKFVWPSEELLQQHYSDLASRPFFPGL 225
           + T  ++KP+ V+      I +R ++ GFK+   K V  S+EL  Q Y +   + FF GL
Sbjct: 673 QSTVAVIKPE-VEPDQRELIKQRIKEAGFKIQLQKEVTLSKELASQFYHEHEGKDFFEGL 731

Query: 226 VKYMSSGPVV 255
             YMSSGP +
Sbjct: 732 TDYMSSGPTM 741



 Score = 43.2 bits (97), Expect = 2e-04
 Identities = 22/72 (30%), Positives = 42/72 (58%)
 Frame = +1

Query: 40  QRERTFIMVKPDGVQRGLVGTIIERFEKKGFKLVGLKFVWPSEELLQQHYSDLASRPFFP 219
           Q +RT  +++PD + R    +I+ + ++ GF++   K +  + E  ++ YS+   + FF 
Sbjct: 536 QLQRTLALIRPDAL-RSRRESIMSKIQEAGFEIAMSKEMHLTREQAEEFYSEHKDQEFFD 594

Query: 220 GLVKYMSSGPVV 255
            LV  MSSGP++
Sbjct: 595 TLVTNMSSGPMM 606



 Score = 32.7 bits (71), Expect = 0.23
 Identities = 21/78 (26%), Positives = 39/78 (50%), Gaps = 6/78 (7%)
 Frame = +3

Query: 237 EFRTCGP---MVWEGLNVVKTGRQMLGATNPADSQ---PGTIRGDLCIQVGRNIIHGSDS 398
           ++ + GP   MV    + V   R ++G  +P  ++   P +IR  L   V +N++HG   
Sbjct: 733 DYMSSGPTMFMVLSKEDAVSGWRSLMGPVDPEQAKEMAPESIRAALGKDVMKNVVHGPSD 792

Query: 399 VESAKKEIGLWFTDKEVV 452
            E A K I  +F + +++
Sbjct: 793 PEKAGKVIKEFFPEAKIL 810



 Score = 29.9 bits (64), Expect = 1.6
 Identities = 19/63 (30%), Positives = 32/63 (50%), Gaps = 4/63 (6%)
 Frame = +3

Query: 276 NVVKTGRQMLG---ATNPADSQPGTIRGDLCIQVGR-NIIHGSDSVESAKKEIGLWFTDK 443
           + ++  R MLG        D  P ++R    ++    N +HGSD+ E+A+KEI  +F  +
Sbjct: 614 DAIEGWRGMLGPKEVEKAKDEAPESLRAQFQVEDSPINPLHGSDTAENAEKEIQKFFPMQ 673

Query: 444 EVV 452
             V
Sbjct: 674 STV 676



 Score = 28.7 bits (61), Expect = 3.7
 Identities = 11/22 (50%), Positives = 15/22 (68%)
 Frame = +3

Query: 375 NIIHGSDSVESAKKEIGLWFTD 440
           N +HG DS ESA +E+  +F D
Sbjct: 501 NAVHGCDSNESAARELAFFFPD 522


>SB_13421| Best HMM Match : Harpin (HMM E-Value=0.62)
          Length = 375

 Score = 28.3 bits (60), Expect = 4.9
 Identities = 18/50 (36%), Positives = 21/50 (42%), Gaps = 2/50 (4%)
 Frame = -2

Query: 332 LRVSWVSCAKHLTASL--HNIKALPYHRTTGPELMYFTRPGKKGRDAKSL 189
           LRVSW S   H  ASL  H   +L  H    P+      P   G+   SL
Sbjct: 226 LRVSWASLKSHGAASLKSHEAASLKSHVAASPKSHGAASPSLMGQHFSSL 275


>SB_56833| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 428

 Score = 27.5 bits (58), Expect = 8.6
 Identities = 17/61 (27%), Positives = 25/61 (40%), Gaps = 4/61 (6%)
 Frame = -2

Query: 263 YHRTTGPELMYFTRPGKKGRDAKSL*CCWRSSSDGHTNF----KPTSLKPFFSKRSIMVP 96
           YH T    L+Y  R        +SL  CW+   D    F    +   LK F   +++ +P
Sbjct: 294 YHHTAPINLLYTLRESLAQLAEESLESCWKRHHDNAELFWKGIEDLGLKMFVKDKNLRLP 353

Query: 95  T 93
           T
Sbjct: 354 T 354


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,495,378
Number of Sequences: 59808
Number of extensions: 391503
Number of successful extensions: 1157
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 1092
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1157
length of database: 16,821,457
effective HSP length: 78
effective length of database: 12,156,433
effective search space used: 1422302661
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)