Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30003
(692 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 129 4e-31
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 129 4e-31
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 94 2e-20
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 81 1e-16
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 34 0.017
SPAC1952.03 |||cysteine protease, OTU family|Schizosaccharomyces... 27 1.9
SPAPB1A10.09 |ase1||microtubule-associated protein Ase1 |Schizos... 27 2.6
SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+, L-... 26 4.5
SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit ... 26 4.5
SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein |Schizo... 26 4.5
SPAC5D6.09c |mug86||acetate transporter |Schizosaccharomyces pom... 25 7.9
SPBC839.08c |its8||pig-N |Schizosaccharomyces pombe|chr 2|||Manual 25 7.9
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 129 bits (311), Expect = 4e-31
Identities = 56/85 (65%), Positives = 66/85 (77%)
Frame = -2
Query: 511 LEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGG 332
+EIQ E A+GGIY VLN++RGHVF E Q GTP++ +KAYLPVNESFGFT +LR T G
Sbjct: 731 VEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNIKAYLPVNESFGFTGELRQATAG 790
Query: 331 QAFPQCVFDHWQVLPGDPCEPQSKP 257
QAFPQ VFDHW + GDP +P SKP
Sbjct: 791 QAFPQLVFDHWSPMSGDPLDPTSKP 815
Score = 89.0 bits (211), Expect = 6e-19
Identities = 42/65 (64%), Positives = 47/65 (72%)
Frame = -3
Query: 690 AKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYL 511
+KEG M EENLR RFNI DV LH DAIHRGGGQIIPT RR +YA L A P + EPV+L
Sbjct: 671 SKEGPMFEENLRSCRFNILDVVLHADAIHRGGGQIIPTARRVVYASTLLASPIIQEPVFL 730
Query: 510 WKFSV 496
+ V
Sbjct: 731 VEIQV 735
Score = 31.1 bits (67), Expect = 0.16
Identities = 16/34 (47%), Positives = 20/34 (58%)
Frame = -1
Query: 305 PLAGPPWRPVRTSEQALNVVQETRKRKGLKEGLP 204
P++G P P Q +V E RKRKGLKE +P
Sbjct: 803 PMSGDPLDPTSKPGQ---IVCEARKRKGLKENVP 833
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 129 bits (311), Expect = 4e-31
Identities = 56/85 (65%), Positives = 66/85 (77%)
Frame = -2
Query: 511 LEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGG 332
+EIQ E A+GGIY VLN++RGHVF E Q GTP++ +KAYLPVNESFGFT +LR T G
Sbjct: 731 VEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNIKAYLPVNESFGFTGELRQATAG 790
Query: 331 QAFPQCVFDHWQVLPGDPCEPQSKP 257
QAFPQ VFDHW + GDP +P SKP
Sbjct: 791 QAFPQLVFDHWSPMSGDPLDPTSKP 815
Score = 89.0 bits (211), Expect = 6e-19
Identities = 42/65 (64%), Positives = 47/65 (72%)
Frame = -3
Query: 690 AKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYL 511
+KEG M EENLR RFNI DV LH DAIHRGGGQIIPT RR +YA L A P + EPV+L
Sbjct: 671 SKEGPMFEENLRSCRFNILDVVLHADAIHRGGGQIIPTARRVVYASTLLASPIIQEPVFL 730
Query: 510 WKFSV 496
+ V
Sbjct: 731 VEIQV 735
Score = 31.1 bits (67), Expect = 0.16
Identities = 16/34 (47%), Positives = 20/34 (58%)
Frame = -1
Query: 305 PLAGPPWRPVRTSEQALNVVQETRKRKGLKEGLP 204
P++G P P Q +V E RKRKGLKE +P
Sbjct: 803 PMSGDPLDPTSKPGQ---IVCEARKRKGLKENVP 833
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 93.9 bits (223), Expect = 2e-20
Identities = 45/97 (46%), Positives = 63/97 (64%)
Frame = -2
Query: 511 LEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGG 332
+E+ P ++ IY +L RRRGHV ++ G+P+++V+A +PV +S GF DLR +T G
Sbjct: 847 VEVHAPADSLPIIYDLLTRRRGHVLQDIPRPGSPLYLVRALIPVIDSCGFETDLRVHTQG 906
Query: 331 QAFPQCVFDHWQVLPGDPCEPQSKP*TLYRKRERGKD 221
QA Q VFDHWQV+PGDP + KP L + RG D
Sbjct: 907 QAMCQMVFDHWQVVPGDPLDKSIKPKPL--EPARGSD 941
Score = 75.8 bits (178), Expect = 6e-15
Identities = 33/59 (55%), Positives = 42/59 (71%)
Frame = -3
Query: 687 KEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYL 511
+EG + +E +R V F + DV L + I+RGGGQIIPT RR Y+ LTA PRLMEPVY+
Sbjct: 788 REGPLCDETIRNVNFRLMDVVLAPEQIYRGGGQIIPTARRVCYSSFLTASPRLMEPVYM 846
Score = 27.9 bits (59), Expect = 1.5
Identities = 15/49 (30%), Positives = 24/49 (48%)
Frame = -1
Query: 359 CRFAFQHRRTGLPAVRIRPLAGPPWRPVRTSEQALNVVQETRKRKGLKE 213
C+ F H + + + P P R S+ A + + +TR+RKGL E
Sbjct: 910 CQMVFDHWQVVPGDPLDKSIKPKPLEPARGSDLARDFLIKTRRRKGLVE 958
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 1000
Score = 81.0 bits (191), Expect = 1e-16
Identities = 35/82 (42%), Positives = 52/82 (63%)
Frame = -2
Query: 523 ACISLEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRS 344
A S ++Q +G +YGV+++RRG V +E GTP FIVKA +PV ESFGF ++
Sbjct: 865 AMYSCDVQATSEVLGRVYGVVSKRRGRVIDEEMKEGTPFFIVKALIPVVESFGFAVEILK 924
Query: 343 NTGGQAFPQCVFDHWQVLPGDP 278
T G A+PQ +F +++L +P
Sbjct: 925 RTSGAAYPQLIFHGFEMLDENP 946
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 34.3 bits (75), Expect = 0.017
Identities = 16/63 (25%), Positives = 35/63 (55%)
Frame = -2
Query: 517 ISLEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNT 338
+++ I P GG+ G L++R+ + + F ++A +P+N F +++D+R+ T
Sbjct: 679 MNVSITAPVEHQGGVIGNLDKRKATIVDSD--TDEDEFTLQAEVPLNSMFSYSSDIRALT 736
Query: 337 GGQ 329
G+
Sbjct: 737 KGK 739
>SPAC1952.03 |||cysteine protease, OTU family|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 324
Score = 27.5 bits (58), Expect = 1.9
Identities = 28/95 (29%), Positives = 41/95 (43%), Gaps = 6/95 (6%)
Frame = -3
Query: 297 RSSLETRANLRASPKRCTGNEKEERIEGRSPRLNSIFGQIVNYIVK-AN*KNVSIRKALH 121
+SSL T+ N PK+ N ++ER+E R + + Q K A+ KN +K
Sbjct: 120 QSSLNTKENTPQQPKKSR-NRQKERLERRKAEMKKMSEQAELESEKMADLKNEE-KKKFS 177
Query: 120 ACLFSTTHVPHHVKAD-----AQVRQQLNMSHSTK 31
L V + AD A + QLN H+ K
Sbjct: 178 KILEEAGLVAVDIPADGNCLFASISHQLNYHHNVK 212
>SPAPB1A10.09 |ase1||microtubule-associated protein Ase1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 731
Score = 27.1 bits (57), Expect = 2.6
Identities = 15/34 (44%), Positives = 20/34 (58%)
Frame = -1
Query: 305 PLAGPPWRPVRTSEQALNVVQETRKRKGLKEGLP 204
PL+ P R V +E ALN++ E +R LKE P
Sbjct: 638 PLSPPKQRVV--NEHALNIMSEKLQRTNLKEQTP 669
>SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+,
L-lysine forming] |Schizosaccharomyces pombe|chr
1|||Manual
Length = 368
Score = 26.2 bits (55), Expect = 4.5
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = +2
Query: 575 VVGMIWPPPLWMASVWSVTS*MLNRTPRKFSSAITPSL 688
V G+ PPPL + S+ + + + + FS A+ PSL
Sbjct: 305 VKGVTTPPPLEVISIDHLPTLLPRESSEAFSEALIPSL 342
>SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit
Sec63 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 611
Score = 26.2 bits (55), Expect = 4.5
Identities = 17/79 (21%), Positives = 34/79 (43%)
Frame = -2
Query: 598 WWPNHSNN*KMLVCMSANCSAPSHGACISLEIQCPEVAVGGIYGVLNRRRGHVFEESQVA 419
W+P + + +S S+ + + E E + ++ LNR++ + F Q+
Sbjct: 234 WFPKMETSLTLDELLSLFASSKELTSLVPNEKNPKEYILKLLFDHLNRKKTNNFNTHQIL 293
Query: 418 GTPMFIVKAYLPVNESFGF 362
++ A L V +FGF
Sbjct: 294 SQSDVVLNALLSVATAFGF 312
>SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 391
Score = 26.2 bits (55), Expect = 4.5
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 1/49 (2%)
Frame = +1
Query: 520 RLHETGLSS*QTCIQASSS-CWNDLATTSMDGISMECYIIDVESNTTQI 663
++ ET L + ++S CW++L+TTS + + II + TT I
Sbjct: 191 QIQETSLGATTQEDESSDGICWDELSTTSPESSKVSEPIIQDNTQTTHI 239
>SPAC5D6.09c |mug86||acetate transporter |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 304
Score = 25.4 bits (53), Expect = 7.9
Identities = 18/60 (30%), Positives = 28/60 (46%)
Frame = +1
Query: 145 VFLICFNYVVYNLSKY*VKSGRPSFNPFLFLVSCTTFRACSEVRTGLQGGPANGRIRTAG 324
++LIC+ + + V+S F+ F+ L C AC+ +RT G P IR G
Sbjct: 191 LYLICWFIFTFLVLLCTVRSTLAFFSLFMSLDVCFLLLACAFLRTS-DGSPNVVLIRVGG 249
>SPBC839.08c |its8||pig-N |Schizosaccharomyces pombe|chr 2|||Manual
Length = 935
Score = 25.4 bits (53), Expect = 7.9
Identities = 9/31 (29%), Positives = 18/31 (58%)
Frame = -2
Query: 532 SHGACISLEIQCPEVAVGGIYGVLNRRRGHV 440
+ GA + I P +A+ +G++N+R G +
Sbjct: 833 TQGALLMYTILVPFIALSAAFGIMNKRLGGI 863
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,860,411
Number of Sequences: 5004
Number of extensions: 62053
Number of successful extensions: 139
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 321951680
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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