Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30002
(630 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 175 3e-45
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 100 3e-22
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 95 7e-21
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 91 1e-19
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 63 3e-11
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 59 6e-10
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 52 9e-08
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 35 0.011
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.079
SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces p... 29 0.42
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 2.2
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 3.9
SPCC757.09c |rnc1||RNA-binding protein that suppresses calcineur... 26 3.9
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 3.9
SPBC36.11 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||M... 26 5.2
SPAC664.03 |||RNA polymerase II associated Paf1 complex |Schizos... 25 6.8
SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc... 25 6.8
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 25 9.0
SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 25 9.0
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 175 bits (427), Expect = 3e-45
Identities = 89/125 (71%), Positives = 95/125 (76%), Gaps = 1/125 (0%)
Frame = +2
Query: 257 DEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKAN 436
DEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+N
Sbjct: 56 DEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSN 115
Query: 437 REKMTQIMFETFNTPAMYVAIQAV-SRCTRPVVPPVSCWTPATVSPTPCPSTRDTHCPHA 613
REKMTQI+FETFN PA YVAIQAV S + + V+ T P PHA
Sbjct: 116 REKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHT-VPIYEGYALPHA 174
Query: 614 ILRLD 628
I+RLD
Sbjct: 175 IMRLD 179
Score = 110 bits (265), Expect = 1e-25
Identities = 48/53 (90%), Positives = 52/53 (98%)
Frame = +3
Query: 96 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYV 254
+EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYV
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYV 54
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 99.5 bits (237), Expect = 3e-22
Identities = 41/97 (42%), Positives = 68/97 (70%)
Frame = +2
Query: 218 RDGRYGTEGLLCRDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHP 397
+ G + ++ DEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P
Sbjct: 43 KTGNVQIKDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRK 102
Query: 398 VLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAV 508
+LLTE P+NP ANREKM + MFE + +YVAIQAV
Sbjct: 103 ILLTEPPMNPVANREKMCETMFERYGFGGVYVAIQAV 139
Score = 48.0 bits (109), Expect = 1e-06
Identities = 21/35 (60%), Positives = 26/35 (74%)
Frame = +3
Query: 102 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 206
E A +V+DNG+G K G+A D+ PR FPSIVGRP
Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36
Score = 48.0 bits (109), Expect = 1e-06
Identities = 19/32 (59%), Positives = 26/32 (81%)
Frame = +1
Query: 508 VSLYASGRTTGIVLDSGDGVSHTVPIYEGYAL 603
+SLYA G ++G+V+DSGDGV+H VP+YE L
Sbjct: 140 LSLYAQGLSSGVVVDSGDGVTHIVPVYESVVL 171
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 95.1 bits (226), Expect = 7e-21
Identities = 39/74 (52%), Positives = 50/74 (67%)
Frame = +2
Query: 287 TLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFE 466
+L YPI HG + NWD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE
Sbjct: 89 SLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFE 148
Query: 467 TFNTPAMYVAIQAV 508
+FN +Y+A+QAV
Sbjct: 149 SFNCAGLYIAVQAV 162
Score = 42.3 bits (95), Expect = 6e-05
Identities = 20/49 (40%), Positives = 31/49 (63%), Gaps = 2/49 (4%)
Frame = +3
Query: 114 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYV 254
+++DNG+G K G+AG+DAP VFP+++ R G G + K SY+
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYM 55
Score = 38.7 bits (86), Expect = 7e-04
Identities = 14/23 (60%), Positives = 19/23 (82%)
Frame = +1
Query: 535 TGIVLDSGDGVSHTVPIYEGYAL 603
TG V+DSGDGV+H +P+ EGY +
Sbjct: 180 TGTVVDSGDGVTHIIPVAEGYVI 202
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 91.1 bits (216), Expect = 1e-19
Identities = 40/84 (47%), Positives = 58/84 (69%), Gaps = 1/84 (1%)
Frame = +2
Query: 260 EAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKAN 436
EAQ+ RG+L ++ PIE GI+ NW DME+IW + + ++ L PEEHP+LLTE PL N
Sbjct: 61 EAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRN 120
Query: 437 REKMTQIMFETFNTPAMYVAIQAV 508
+EK+ + +ET N PA+ ++Q V
Sbjct: 121 KEKIAEYFYETLNVPALSFSLQPV 144
Score = 61.3 bits (142), Expect = 1e-10
Identities = 26/47 (55%), Positives = 34/47 (72%)
Frame = +3
Query: 114 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYV 254
+ +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +V
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFV 58
Score = 55.2 bits (127), Expect = 7e-09
Identities = 21/33 (63%), Positives = 31/33 (93%)
Frame = +1
Query: 508 VSLYASGRTTGIVLDSGDGVSHTVPIYEGYALP 606
++LYAS RTTGIVL+ GDG++H+VPIY+G+++P
Sbjct: 145 LALYASARTTGIVLECGDGLTHSVPIYDGFSIP 177
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 63.3 bits (147), Expect = 3e-11
Identities = 29/73 (39%), Positives = 41/73 (56%)
Frame = +2
Query: 284 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 463
+ +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MF
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122
Query: 464 ETFNTPAMYVAIQ 502
E+ PA Y+A Q
Sbjct: 123 ESLRCPATYLAKQ 135
Score = 40.3 bits (90), Expect = 2e-04
Identities = 16/34 (47%), Positives = 24/34 (70%)
Frame = +3
Query: 99 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 200
+EV+A+V+D GS + GF+G+D P+ V PS G
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42
Score = 25.0 bits (52), Expect = 9.0
Identities = 11/29 (37%), Positives = 17/29 (58%)
Frame = +1
Query: 517 YASGRTTGIVLDSGDGVSHTVPIYEGYAL 603
+ASG+ T ++D G S IY+G+ L
Sbjct: 141 FASGKGTACLVDIGAERSSVSAIYDGFVL 169
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 58.8 bits (136), Expect = 6e-10
Identities = 25/70 (35%), Positives = 42/70 (60%)
Frame = +2
Query: 299 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNT 478
P E +++NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE +N
Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144
Query: 479 PAMYVAIQAV 508
P++ I +
Sbjct: 145 PSVAYGIDGL 154
Score = 35.9 bits (79), Expect = 0.005
Identities = 15/32 (46%), Positives = 22/32 (68%)
Frame = +3
Query: 114 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 209
LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
Score = 26.6 bits (56), Expect = 3.0
Identities = 9/21 (42%), Positives = 15/21 (71%)
Frame = +1
Query: 532 TTGIVLDSGDGVSHTVPIYEG 594
++GIVL+ G+ SH +P+ G
Sbjct: 165 SSGIVLNLGNAASHVIPVLNG 185
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 51.6 bits (118), Expect = 9e-08
Identities = 21/67 (31%), Positives = 39/67 (58%)
Frame = +2
Query: 308 HGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAM 487
+GI+ +W+ W +L+V P E+ +++TE NP++ R+++ + FE + PA
Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133
Query: 488 YVAIQAV 508
Y+ QAV
Sbjct: 134 YLTKQAV 140
Score = 36.7 bits (81), Expect = 0.003
Identities = 14/34 (41%), Positives = 23/34 (67%)
Frame = +3
Query: 99 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 200
EE+ +LV+D GS + G+AG+++P + PS G
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 34.7 bits (76), Expect = 0.011
Identities = 21/72 (29%), Positives = 32/72 (44%), Gaps = 4/72 (5%)
Frame = +2
Query: 299 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 466
PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 467 TFNTPAMYVAIQ 502
P +A +
Sbjct: 172 ECQVPGFTIAYE 183
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 31.9 bits (69), Expect = 0.079
Identities = 19/53 (35%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
Frame = +2
Query: 359 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTPAM---YVAIQ 502
+Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 277
Score = 29.5 bits (63), Expect = 0.42
Identities = 19/66 (28%), Positives = 29/66 (43%)
Frame = -3
Query: 331 IPVSDDSVFDGVFEGQDTSFALCLIST*ESFCPIPTITP*WRGLPTIEGNTARGASSPAK 152
+P + +V +TSFAL S S PT + P +E + +S+PA
Sbjct: 63 LPTQEAAVETNASASHETSFALPTTSPAASLSISPTKSAAVSSEPNVEADVKSLSSTPAA 122
Query: 151 PALHIP 134
P L+ P
Sbjct: 123 PQLNSP 128
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 27.1 bits (57), Expect = 2.2
Identities = 18/54 (33%), Positives = 22/54 (40%), Gaps = 1/54 (1%)
Frame = -3
Query: 253 T*ESFCPI-PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 95
T S P+ PT+ P P N S P PL+TTN TS+S
Sbjct: 402 TGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 3.9
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +2
Query: 401 LLTEAPLNPKANREKMTQIMFETFN 475
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
>SPCC757.09c |rnc1||RNA-binding protein that suppresses calcineurin
deletion Rnc1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 398
Score = 26.2 bits (55), Expect = 3.9
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = +2
Query: 506 VSRCTRPVVPPVSCWTPATVSPTPCPST 589
VSR T+P+ S +P VSP PST
Sbjct: 263 VSRLTQPLPSLASTASPQQVSPPAAPST 290
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.2 bits (55), Expect = 3.9
Identities = 13/31 (41%), Positives = 16/31 (51%)
Frame = -3
Query: 235 PIPTITP*WRGLPTIEGNTARGASSPAKPAL 143
P+PT P LPT NT + P+ PAL
Sbjct: 74 PVPTGAP---SLPTSSNNTQQAEERPSMPAL 101
>SPBC36.11 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 343
Score = 25.8 bits (54), Expect = 5.2
Identities = 16/38 (42%), Positives = 21/38 (55%)
Frame = -2
Query: 617 GWRGGSAYPS*MGTVWETPSPESSTIPVVRPDAYSETR 504
G+RGG S T W + SP +STIP Y++TR
Sbjct: 158 GYRGGYQMHS---TPWAS-SPRNSTIPQRSQSFYNDTR 191
>SPAC664.03 |||RNA polymerase II associated Paf1 complex
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 457
Score = 25.4 bits (53), Expect = 6.8
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = -1
Query: 567 DTVAGVQHDTGGTTGRVQRDTAWMATYMAGV 475
D G+ D G TG + DT+WM + ++ V
Sbjct: 58 DIELGMPLDLAGITGFFEGDTSWMHSDLSSV 88
>SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 721
Score = 25.4 bits (53), Expect = 6.8
Identities = 15/52 (28%), Positives = 30/52 (57%), Gaps = 1/52 (1%)
Frame = +1
Query: 454 DH-VRNIQHARHVRRHPSRVSLYASGRTTGIVLDSGDGVSHTVPIYEGYALP 606
DH V+N A ++R++ + + +A G+ +G G+ H + + +GYA+P
Sbjct: 126 DHDVQNKSEA-NLRKYKN-IESFAKGQGIDFY-PAGRGIGHQIMVEQGYAMP 174
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 25.0 bits (52), Expect = 9.0
Identities = 14/45 (31%), Positives = 19/45 (42%)
Frame = +2
Query: 464 ETFNTPAMYVAIQAVSRCTRPVVPPVSCWTPATVSPTPCPSTRDT 598
+T ++ + + T P S TPA V P P PST T
Sbjct: 815 KTSQASSILPTVPRTTSYTSPYATTSSHITPADVHPLPPPSTSTT 859
>SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 422
Score = 25.0 bits (52), Expect = 9.0
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = -2
Query: 626 PDAGWRGGSAYPS 588
P AG+RGGSA PS
Sbjct: 139 PPAGYRGGSARPS 151
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,831,343
Number of Sequences: 5004
Number of extensions: 62380
Number of successful extensions: 213
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 209
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 279695522
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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