BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV11002 (707 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 101 1e-22 SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 101 1e-22 SPCC777.13 |vps35||retromer complex subunit Vps35|Schizosaccharo... 29 0.65 SPAC9E9.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 28 1.1 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 27 2.6 SPAC22G7.08 |ppk8||serine/threonine protein kinase Ppk8 |Schizos... 27 3.5 SPAC8F11.06 |||nuclear envelope protein |Schizosaccharomyces pom... 26 4.6 SPAC8E11.01c ||SPAC959.01|beta-fructofuranosidase|Schizosaccharo... 25 8.0 >SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 101 bits (242), Expect = 1e-22 Identities = 45/84 (53%), Positives = 59/84 (70%) Frame = +3 Query: 3 KGPLIIFNKDQGLTRAFRNIPGVEXXXXXXXXXXXXAPGGHLGRFVIWTQSAFGRLDPLF 182 +GPL++FN+D G+ +AFRNIPGVE APGGHLGRFVIWT+SAFG LD +F Sbjct: 205 RGPLVVFNEDAGIVKAFRNIPGVEIVNVRRLNLLQLAPGGHLGRFVIWTKSAFGLLDSVF 264 Query: 183 GSWKTPSKQKKNFNLPQPKMANTD 254 GS ++ KKN+ LP+ ++N D Sbjct: 265 GSTTEAAQLKKNYFLPENIISNAD 288 Score = 44.8 bits (101), Expect = 1e-05 Identities = 28/68 (41%), Positives = 40/68 (58%), Gaps = 2/68 (2%) Frame = +2 Query: 254 LTRLLKSDEIRKVLRAPN-KRVIRA-TRKLNPLTNNKAMLKLNPYAAVLKRKAILELRRR 427 +TRL+ SDEI+ +++A RV RA +K NPL N + +LNPYA KA ++L Sbjct: 289 VTRLINSDEIQSIVKAAGPSRVKRAHVQKKNPLKNKAVLARLNPYAKAY--KANVKLNTG 346 Query: 428 KNLKALAD 451 K KA + Sbjct: 347 KTPKAAGE 354 >SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 101 bits (241), Expect = 1e-22 Identities = 45/84 (53%), Positives = 59/84 (70%) Frame = +3 Query: 3 KGPLIIFNKDQGLTRAFRNIPGVEXXXXXXXXXXXXAPGGHLGRFVIWTQSAFGRLDPLF 182 +GPL++FN+D G+ +AFRNIPGVE APGGHLGRFVIWT+SAFG LD +F Sbjct: 205 RGPLVVFNEDTGIVKAFRNIPGVEIVNVRRLNLLQLAPGGHLGRFVIWTKSAFGLLDSVF 264 Query: 183 GSWKTPSKQKKNFNLPQPKMANTD 254 GS ++ KKN+ LP+ ++N D Sbjct: 265 GSTTEVAQLKKNYFLPENIISNAD 288 Score = 44.4 bits (100), Expect = 2e-05 Identities = 27/68 (39%), Positives = 40/68 (58%), Gaps = 2/68 (2%) Frame = +2 Query: 254 LTRLLKSDEIRKVLRAPN-KRVIRA-TRKLNPLTNNKAMLKLNPYAAVLKRKAILELRRR 427 +TRL+ SDEI+ +++A RV RA +K NPL N + +LNPYA KA +++ Sbjct: 289 VTRLINSDEIQSIVKAAGPSRVKRAHVQKKNPLKNKAVLSRLNPYAKAY--KANVKINSE 346 Query: 428 KNLKALAD 451 K KA + Sbjct: 347 KTPKAAGE 354 >SPCC777.13 |vps35||retromer complex subunit Vps35|Schizosaccharomyces pombe|chr 3|||Manual Length = 785 Score = 29.1 bits (62), Expect = 0.65 Identities = 14/43 (32%), Positives = 23/43 (53%) Frame = -3 Query: 546 ESLLDVLASPLAYFSAFIAGFRLDSFNPLFSASARAFKFFLLR 418 +SL +L PL YF +F L +F P+F+A ++ + R Sbjct: 382 KSLCAILLLPLTYFPSFSYCLELQNFLPVFNAQDPNLRYDIAR 424 >SPAC9E9.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 313 Score = 28.3 bits (60), Expect = 1.1 Identities = 20/52 (38%), Positives = 28/52 (53%) Frame = -2 Query: 541 PP*CSCVSSRVFLSLHSGVSLRQLQSTLLGISKSLQVLPSAQL*DSFPLQHG 386 PP S SS + LSL S SL+Q + T +S + + + S QL P+ HG Sbjct: 134 PPNGSESSSPIPLSLLSTSSLQQRKITPSNLSNTSKPMDSKQLERLIPVPHG 185 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 27.1 bits (57), Expect = 2.6 Identities = 27/94 (28%), Positives = 45/94 (47%) Frame = -2 Query: 520 SSRVFLSLHSGVSLRQLQSTLLGISKSLQVLPSAQL*DSFPLQHGRVRIEFQHRLVISEW 341 S+ V L HSG + S + I+ S S++ P +V +EF+ RL I + Sbjct: 165 STAVLLKHHSG---SKSASAISNIAPSHSNSTSSRRPYIHPAFLSQVAVEFRKRLNIGDR 221 Query: 340 VQFACSTDHAFVGSTEDLPDLIRLEKTCESVLAI 239 V+ AF+GS E + L+ + +T + LA+ Sbjct: 222 VKDGLLYKDAFLGS-EAVDVLMHIVRTTDRNLAL 254 >SPAC22G7.08 |ppk8||serine/threonine protein kinase Ppk8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 26.6 bits (56), Expect = 3.5 Identities = 14/39 (35%), Positives = 18/39 (46%), Gaps = 2/39 (5%) Frame = +3 Query: 189 WKTPSKQKKNFNL--PQPKMANTDSHVFSSLMRSGRSSV 299 WK P K FNL Q NT S + SL + R ++ Sbjct: 444 WKVPHTSDKRFNLYVTQRNDPNTKSQLIESLPMNSRDTI 482 >SPAC8F11.06 |||nuclear envelope protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 297 Score = 26.2 bits (55), Expect = 4.6 Identities = 24/83 (28%), Positives = 37/83 (44%), Gaps = 6/83 (7%) Frame = +2 Query: 146 DSVRIRQA*PLIRVMEDTIKTKEELQPAPTEDGQH*LTRLLKSDEIRKVLR--APN---K 310 DSV P +++ KT+ E P P + + LK +R+ LR PN Sbjct: 16 DSVLNTPIRPKYEILKSKKKTQNENDPEPMDISMSPDEKNLKKSTVRRKLRKSKPNSSSN 75 Query: 311 RVIRATRKLNPLTN-NKAMLKLN 376 +V TR L +N + A++K N Sbjct: 76 QVSSRTRALTKRSNSSNAIIKAN 98 >SPAC8E11.01c ||SPAC959.01|beta-fructofuranosidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 508 Score = 25.4 bits (53), Expect = 8.0 Identities = 10/39 (25%), Positives = 20/39 (51%) Frame = -2 Query: 385 RVRIEFQHRLVISEWVQFACSTDHAFVGSTEDLPDLIRL 269 + ++ F H L + +WV+ + +G + PD +RL Sbjct: 171 KYKVLFYHSLNLRDWVKLSEFGSAGVLGYQYECPDFVRL 209 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,464,034 Number of Sequences: 5004 Number of extensions: 46910 Number of successful extensions: 138 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 131 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 136 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 329179816 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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