SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV10997
         (788 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC22E12.02 |||RNA-binding protein|Schizosaccharomyces pombe|ch...    34   0.027
SPBC16E9.12c |pab2||poly|Schizosaccharomyces pombe|chr 2|||Manual      33   0.035
SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr ...    33   0.062
SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schi...    32   0.11 
SPAC13D6.02c |byr3||zinc finger protein Byr3|Schizosaccharomyces...    31   0.19 
SPAC16.02c |srp2||mRNA export factor Srp2|Schizosaccharomyces po...    29   0.57 
SPBP22H7.02c |||RNA-binding protein Mrd1 |Schizosaccharomyces po...    29   1.0  
SPCC74.09 |mug24||RNA-binding protein, rrm type|Schizosaccharomy...    29   1.0  
SPBC887.05c |cwf29||RNA-binding protein Cwf29|Schizosaccharomyce...    28   1.3  
SPBC56F2.08c |||RNA-binding protein|Schizosaccharomyces pombe|ch...    28   1.3  
SPAC16E8.06c |nop12||RNA-binding protein Nop12|Schizosaccharomyc...    27   2.3  
SPCC1827.05c |||nucleolar RNA-binding protein NIFK |Schizosaccha...    27   2.3  
SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase Wis4|Schizo...    27   2.3  
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M...    27   3.1  
SPBC3B9.11c |ctf1||mRNA cleavage and polyadenylation specificity...    27   3.1  
SPBC11C11.08 |srp1||SR family protein Srp1|Schizosaccharomyces p...    27   3.1  
SPAC57A7.04c |pabp||mRNA export shuttling protein |Schizosacchar...    27   4.0  
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc...    26   5.4  
SPBP8B7.15c |||ubiquitin-protein ligase E3 RBBP6 family |Schizos...    26   7.1  
SPAC328.05 |||RNA-binding protein involved in export of mRNAs|Sc...    26   7.1  
SPAC13G6.05c |||TRAPP complex subunit Bet3 |Schizosaccharomyces ...    26   7.1  
SPBC4F6.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ...    25   9.4  
SPBC2F12.05c |||sterol binding ankyrin repeat protein|Schizosacc...    25   9.4  
SPCC320.07c |mde7||RNA-binding protein Mde7|Schizosaccharomyces ...    25   9.4  

>SPAC22E12.02 |||RNA-binding protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 219

 Score = 33.9 bits (74), Expect = 0.027
 Identities = 19/49 (38%), Positives = 27/49 (55%), Gaps = 4/49 (8%)
 Frame = +1

Query: 115 KVYVGDLGNNASKPELEDAFSYYGPLRNVWVARN----PPGFAFVEFED 249
           +++VG+LGN+ +   L  AFS Y  L    V R+      GF FV F+D
Sbjct: 31  RLFVGNLGNDVNDESLYQAFSEYPSLVKTKVVRDREGKTRGFGFVSFKD 79


>SPBC16E9.12c |pab2||poly|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 166

 Score = 33.5 bits (73), Expect = 0.035
 Identities = 16/53 (30%), Positives = 30/53 (56%), Gaps = 5/53 (9%)
 Frame = +1

Query: 118 VYVGDLGNNASKPELEDAFSYYGPLRNVWV-----ARNPPGFAFVEFEDPRML 261
           VYVG++  + +  EL+  F+  G +  V +       +P GFA++EF +P ++
Sbjct: 57  VYVGNVDYSVTPEELQSHFASCGSVNRVTILCDKFTGHPKGFAYIEFSEPSLV 109


>SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 422

 Score = 32.7 bits (71), Expect = 0.062
 Identities = 15/52 (28%), Positives = 28/52 (53%), Gaps = 5/52 (9%)
 Frame = +1

Query: 112 CKVYVGDLGNNASKPELEDAFSYYGPLRNVWV-----ARNPPGFAFVEFEDP 252
           C VYVG++    ++ ++ D F   GP+++  +     +  P G+ F E+ DP
Sbjct: 5   CVVYVGNIPYEMAEEQVIDIFKQSGPVKSFQLVIDPESGQPKGYGFCEYHDP 56


>SPBC646.12c |gap1|src1, sar1|GTPase activating protein
           Gap1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 766

 Score = 31.9 bits (69), Expect = 0.11
 Identities = 16/52 (30%), Positives = 28/52 (53%), Gaps = 1/52 (1%)
 Frame = -2

Query: 253 VDLRIQQMRSLEDFLQPTHSSV-DHNMKRHLQVQVY*HYSPNLQHKLCSHHS 101
           +D   +++R+  D LQ  H ++ DHN     Q+Q+Y  Y  N + ++   HS
Sbjct: 595 LDALYERIRAERDALQDVHRAICDHNEYLQTQLQIYGSYLNNARSQIKPSHS 646


>SPAC13D6.02c |byr3||zinc finger protein Byr3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 179

 Score = 31.1 bits (67), Expect = 0.19
 Identities = 9/14 (64%), Positives = 12/14 (85%)
 Frame = +3

Query: 402 CYDCGDRGHYARDC 443
           CY+CG+ GH AR+C
Sbjct: 19  CYNCGENGHQAREC 32



 Score = 29.9 bits (64), Expect = 0.43
 Identities = 10/14 (71%), Positives = 10/14 (71%)
 Frame = +3

Query: 402 CYDCGDRGHYARDC 443
           CY CG  GH ARDC
Sbjct: 118 CYACGSYGHQARDC 131



 Score = 29.1 bits (62), Expect = 0.76
 Identities = 10/14 (71%), Positives = 10/14 (71%)
 Frame = +3

Query: 402 CYDCGDRGHYARDC 443
           CY CG  GH ARDC
Sbjct: 85  CYKCGRVGHIARDC 98



 Score = 28.3 bits (60), Expect = 1.3
 Identities = 9/14 (64%), Positives = 9/14 (64%)
 Frame = +3

Query: 402 CYDCGDRGHYARDC 443
           CY CG  GH  RDC
Sbjct: 60  CYACGTAGHLVRDC 73


>SPAC16.02c |srp2||mRNA export factor Srp2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 365

 Score = 29.5 bits (63), Expect = 0.57
 Identities = 15/49 (30%), Positives = 27/49 (55%)
 Frame = +1

Query: 109 DCKVYVGDLGNNASKPELEDAFSYYGPLRNVWVARNPPGFAFVEFEDPR 255
           + +++VG +   A++ ++ D F  YG + +  +     GF FVE ED R
Sbjct: 3   ETRLFVGRIPPQATREDMMDFFKGYGQILDCKLMN---GFGFVEVEDAR 48


>SPBP22H7.02c |||RNA-binding protein Mrd1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 833

 Score = 28.7 bits (61), Expect = 1.0
 Identities = 13/50 (26%), Positives = 28/50 (56%), Gaps = 5/50 (10%)
 Frame = +1

Query: 115 KVYVGDLGNNASKPELEDAFSYYGPLRNVWV-----ARNPPGFAFVEFED 249
           ++++ +L  + ++ +L+  F  +G L  V +       NP GFA+++F D
Sbjct: 324 RLFLRNLTYSCAEDDLKSLFGPFGQLEQVHMPIDKKTNNPKGFAYIDFHD 373


>SPCC74.09 |mug24||RNA-binding protein, rrm type|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 654

 Score = 28.7 bits (61), Expect = 1.0
 Identities = 12/42 (28%), Positives = 26/42 (61%)
 Frame = +1

Query: 118 VYVGDLGNNASKPELEDAFSYYGPLRNVWVARNPPGFAFVEF 243
           V++G+L ++  + E+E+AF  +G + ++ +  +    AFV F
Sbjct: 301 VFIGNLPSSYHEKEIEEAFGKFGKIEHIKIL-SKKNIAFVHF 341


>SPBC887.05c |cwf29||RNA-binding protein Cwf29|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 217

 Score = 28.3 bits (60), Expect = 1.3
 Identities = 18/64 (28%), Positives = 35/64 (54%), Gaps = 6/64 (9%)
 Frame = +1

Query: 82  ASSTMSRYGDCK-VYVGDLGNNASKPELEDAFSYYGPLRNVWVARNPP-----GFAFVEF 243
           +SS    Y D   +Y+G+L  + ++ ++   FS +G   ++ + R+       GFAF+++
Sbjct: 20  SSSWHQDYSDSAYIYIGNLDFDLNEDDILCVFSEFGEPVDINLVRDKETGKSKGFAFLKY 79

Query: 244 EDPR 255
           ED R
Sbjct: 80  EDQR 83


>SPBC56F2.08c |||RNA-binding protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 661

 Score = 28.3 bits (60), Expect = 1.3
 Identities = 13/43 (30%), Positives = 23/43 (53%)
 Frame = +1

Query: 118 VYVGDLGNNASKPELEDAFSYYGPLRNVWVARNPPGFAFVEFE 246
           +YV +L    S   +   FS YG ++++W+  +P   A V +E
Sbjct: 2   LYVSNLPVGTSSSAIHALFSAYGNVKDIWML-SPDNSAIVSYE 43


>SPAC16E8.06c |nop12||RNA-binding protein Nop12|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 438

 Score = 27.5 bits (58), Expect = 2.3
 Identities = 19/66 (28%), Positives = 31/66 (46%), Gaps = 5/66 (7%)
 Frame = +1

Query: 67  LTIQKASSTMSRYGDCKVYVGDLGNNASKPELEDAFSYYGPLRNVWVARNP-----PGFA 231
           L +   S  M +     V+VG+L   A +  L   F   G +  V + R+P      GFA
Sbjct: 255 LRVDSVSHPMPQDTKRCVFVGNLAFEAEEEPLWRYFGDCGSIDYVRIVRDPKTNLGKGFA 314

Query: 232 FVEFED 249
           +++F+D
Sbjct: 315 YIQFKD 320


>SPCC1827.05c |||nucleolar RNA-binding protein NIFK
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 276

 Score = 27.5 bits (58), Expect = 2.3
 Identities = 15/48 (31%), Positives = 27/48 (56%), Gaps = 5/48 (10%)
 Frame = +1

Query: 118 VYVGDLGNNASKPELEDAFSYYGPLRNVWVARN-----PPGFAFVEFE 246
           +YVG L +   + ++   FS +GP+  + ++RN        +AF+EFE
Sbjct: 107 LYVGRLPHGFYEKQMRMYFSQFGPVLRLRMSRNRKTGSSKHYAFIEFE 154


>SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase
            Wis4|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1401

 Score = 27.5 bits (58), Expect = 2.3
 Identities = 11/30 (36%), Positives = 15/30 (50%)
 Frame = -3

Query: 321  FLHGLYDRKSSVHPNHVLRLQHPWIFEFNK 232
            F+   ++R     P  V  L HPWI +F K
Sbjct: 1282 FIEQCFERDPEQRPRAVDLLTHPWITDFRK 1311


>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 500

 Score = 27.1 bits (57), Expect = 3.1
 Identities = 17/56 (30%), Positives = 23/56 (41%), Gaps = 5/56 (8%)
 Frame = +1

Query: 112 CKVYVGDLGNNASKPELEDAFSYYGPLRNVWV-----ARNPPGFAFVEFEDPRMLK 264
           C V+VG L  N     L   F  YG +    V     +    G+ +V+FE P   K
Sbjct: 263 CTVFVGRLSWNVDDQWLGQEFEEYGTIVGARVIMDGQSGRSKGYGYVDFETPEAAK 318


>SPBC3B9.11c |ctf1||mRNA cleavage and polyadenylation specificity
           factor complex subunit Ctf1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 363

 Score = 27.1 bits (57), Expect = 3.1
 Identities = 13/49 (26%), Positives = 27/49 (55%), Gaps = 5/49 (10%)
 Frame = +1

Query: 118 VYVGDLGNNASKPELEDAFSYYGPLRNVWVARNP-----PGFAFVEFED 249
           V+VG++  + S+ ++ + F+  GP++   +  +P      G+ F EF D
Sbjct: 9   VFVGNIPYDVSEQQMTEIFNQVGPVKTFKLVLDPETGSGKGYGFCEFFD 57


>SPBC11C11.08 |srp1||SR family protein Srp1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 275

 Score = 27.1 bits (57), Expect = 3.1
 Identities = 21/64 (32%), Positives = 27/64 (42%), Gaps = 4/64 (6%)
 Frame = +1

Query: 94  MSRYGDCKVYVGDLGNNASKPELEDAFSYYGPLRNVWV----ARNPPGFAFVEFEDPRML 261
           MSR     +YV    +     EL   F  +GPL    +     R    FAFVE+ED R  
Sbjct: 1   MSRRSLRTLYVTGFRDGMRARELAYEFEPFGPLIRCDIPIPRTRTSRPFAFVEYEDSRDA 60

Query: 262 KTQY 273
           +  Y
Sbjct: 61  EDAY 64


>SPAC57A7.04c |pabp||mRNA export shuttling protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 653

 Score = 26.6 bits (56), Expect = 4.0
 Identities = 14/49 (28%), Positives = 28/49 (57%), Gaps = 5/49 (10%)
 Frame = +1

Query: 118 VYVGDLGNNASKPELEDAFSYYGPLRNVWVARNPP-----GFAFVEFED 249
           +YVG+L  + ++  L + F+  GP+ ++ V R+       G+A+V F +
Sbjct: 82  LYVGELDPSVTEAMLFELFNSIGPVASIRVCRDAVTRRSLGYAYVNFHN 130


>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 587

 Score = 26.2 bits (55), Expect = 5.4
 Identities = 9/14 (64%), Positives = 9/14 (64%)
 Frame = +3

Query: 402 CYDCGDRGHYARDC 443
           C  CG  GH ARDC
Sbjct: 336 CRHCGSIGHIARDC 349


>SPBP8B7.15c |||ubiquitin-protein ligase E3 RBBP6 family
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 482

 Score = 25.8 bits (54), Expect = 7.1
 Identities = 7/14 (50%), Positives = 10/14 (71%)
 Frame = +3

Query: 402 CYDCGDRGHYARDC 443
           CY CG +GH+ + C
Sbjct: 185 CYRCGQKGHWIQAC 198


>SPAC328.05 |||RNA-binding protein involved in export of
           mRNAs|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 434

 Score = 25.8 bits (54), Expect = 7.1
 Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 4/51 (7%)
 Frame = +1

Query: 109 DCKVYVGDLGNNASKPELEDAFSYYGPLRNVWVARNP----PGFAFVEFED 249
           DC +YVG+L    S   L D F+  G +    +A  P     GF  V+FE+
Sbjct: 280 DC-IYVGNLPWATSDRNLLDLFTDIGSVIRARIAYEPTGRSKGFGVVQFEN 329


>SPAC13G6.05c |||TRAPP complex subunit Bet3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 245

 Score = 25.8 bits (54), Expect = 7.1
 Identities = 11/33 (33%), Positives = 17/33 (51%)
 Frame = -1

Query: 317 YTGSTTANRPSIQTTYCVFSIRGSSNSTNAKPG 219
           Y+    A  P++ T  C+F ++ S N T   PG
Sbjct: 144 YSAQVIAQCPNLPT--CIFHVKFSPNPTQTSPG 174


>SPBC4F6.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 674

 Score = 25.4 bits (53), Expect = 9.4
 Identities = 14/47 (29%), Positives = 24/47 (51%), Gaps = 5/47 (10%)
 Frame = +1

Query: 118 VYVGDLGNNASKPELEDAFSYYGPLRNVWVARNPP-----GFAFVEF 243
           ++V +L     + +L + FS  GP+++  V  NP      G+ FV F
Sbjct: 7   LFVRNLAFQTKQDDLTNFFSDVGPIKHAVVVTNPETGENRGYGFVTF 53


>SPBC2F12.05c |||sterol binding ankyrin repeat
            protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1310

 Score = 25.4 bits (53), Expect = 9.4
 Identities = 9/25 (36%), Positives = 16/25 (64%)
 Frame = -3

Query: 387  AEEADANEVVDPGCHNRDPH*TFLH 313
            AE+ +  E++D   HN+DP+   L+
Sbjct: 970  AEDMEYTELLDQASHNKDPYQRILY 994


>SPCC320.07c |mde7||RNA-binding protein Mde7|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 761

 Score = 25.4 bits (53), Expect = 9.4
 Identities = 17/46 (36%), Positives = 24/46 (52%), Gaps = 2/46 (4%)
 Frame = +1

Query: 118 VYVGDLGNNASKPELEDAFSYYGPLRNVW--VARNPPGFAFVEFED 249
           +YVG+L N   + +L  AFS     R +   +  N P   FVEFE+
Sbjct: 604 IYVGNLSNPDQEKKLRLAFSKEKGYRRLCFKIKGNSP-MCFVEFEE 648


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,778,938
Number of Sequences: 5004
Number of extensions: 53289
Number of successful extensions: 157
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 383374054
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -