BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10968X (566 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 42 2e-04 At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 41 5e-04 At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 38 0.006 At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 37 0.011 At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 35 0.033 At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 34 0.058 At2g27980.1 68415.m03391 expressed protein 30 1.2 At1g78910.1 68414.m09199 pseudouridine synthase family protein l... 29 2.2 At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 29 2.9 At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containi... 27 6.6 At1g34180.1 68414.m04239 no apical meristem (NAM) family protein... 27 6.6 At4g02400.1 68417.m00324 U3 ribonucleoprotein (Utp) family prote... 27 8.7 At2g01960.1 68415.m00131 expressed protein 27 8.7 >At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein (HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853) (GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res. 17 (19), 7995 (1989)) Length = 157 Score = 42.3 bits (95), Expect = 2e-04 Identities = 27/78 (34%), Positives = 44/78 (56%), Gaps = 8/78 (10%) Frame = +1 Query: 295 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 450 D+ EE+ V+ DG I+ + G+ +EEK D+ + R +FTRR+ LPE E Sbjct: 66 DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125 Query: 451 SVESRLSSDGVLSVIAPR 504 +++ + +GVLSV P+ Sbjct: 126 EIKASM-ENGVLSVTVPK 142 >At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HSP17.4-CI) identical to 17.4 kDa class I heat shock protein SP:P19036 from [Arabidopsis thaliana] Length = 156 Score = 41.1 bits (92), Expect = 5e-04 Identities = 28/78 (35%), Positives = 42/78 (53%), Gaps = 8/78 (10%) Frame = +1 Query: 295 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 450 DV EE+ V+ DG I+ + G+ +EEK D + R +F RR+ LPE E Sbjct: 65 DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVE 124 Query: 451 SVESRLSSDGVLSVIAPR 504 V++ + +GVLSV P+ Sbjct: 125 EVKASM-ENGVLSVTVPK 141 >At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HSP18.1-CI) identical to 18.2 kDa class I heat shock protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana]; contains Pfam profile: PF00011 Hsp20/alpha crystallin family Length = 161 Score = 37.5 bits (83), Expect = 0.006 Identities = 25/88 (28%), Positives = 48/88 (54%), Gaps = 8/88 (9%) Frame = +1 Query: 295 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 450 D+ EE+ V+ D ++ + G+ +EEK D+ + R +F RR+ LPE E Sbjct: 68 DLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKME 127 Query: 451 SVESRLSSDGVLSVIAPRKGRQQWRVNA 534 V++ + +GVL+V+ P+ ++ +V + Sbjct: 128 EVKATM-ENGVLTVVVPKAPEKKPQVKS 154 >At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein (HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha crystallin family; identified in Scharf, K-D., et al, Cell Stress & Chaperones (2001) 6: 225-237. Length = 153 Score = 36.7 bits (81), Expect = 0.011 Identities = 25/78 (32%), Positives = 42/78 (53%), Gaps = 8/78 (10%) Frame = +1 Query: 295 DVQHFAPEEISVKTA-DGYIVVEG-KHEEKKDQHGYISR------QFTRRYALPEGCTAE 450 D+ EE+ V+ D + + G +H EK+D++ R QFTRR+ LPE + Sbjct: 62 DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121 Query: 451 SVESRLSSDGVLSVIAPR 504 V++ + +GVL+V P+ Sbjct: 122 QVKAAM-ENGVLTVTVPK 138 >At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (HSP22.0-ER) identical to endomembrane-localized small heat shock protein GI:511795 from [Arabidopsis thaliana] Length = 195 Score = 35.1 bits (77), Expect = 0.033 Identities = 25/85 (29%), Positives = 47/85 (55%), Gaps = 8/85 (9%) Frame = +1 Query: 262 KSDKDKFQVNLDVQHFAPEEISVKTAD-GYIVVEG---KHEEKK-DQHGYISR---QFTR 417 K + ++ LD+ +E+ ++ + G + V G + EEKK DQ + R +F R Sbjct: 76 KETAEGHEIMLDIPGLKKDEVKIEVEENGVLRVSGERKREEEKKGDQWHRVERSYGKFWR 135 Query: 418 RYALPEGCTAESVESRLSSDGVLSV 492 ++ LP+ ESV+++L +GVL++ Sbjct: 136 QFKLPDNVDMESVKAKL-ENGVLTI 159 >At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HSP17.8-CI) similar to 17.5 kDa class I heat shock protein SP:P04793 from [Glycine max]; contains Pfam PF00011: Hsp20/alpha crystallin family Length = 157 Score = 34.3 bits (75), Expect = 0.058 Identities = 23/78 (29%), Positives = 42/78 (53%), Gaps = 8/78 (10%) Frame = +1 Query: 295 DVQHFAPEEISVKTADGYIV-VEGKH----EEKKDQHGYISR---QFTRRYALPEGCTAE 450 D+ EE+ V+ D ++ + G+ EEK+D + R QF+R++ LPE + Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123 Query: 451 SVESRLSSDGVLSVIAPR 504 V++ + +GVL+V P+ Sbjct: 124 QVKASM-ENGVLTVTVPK 140 >At2g27980.1 68415.m03391 expressed protein Length = 1037 Score = 29.9 bits (64), Expect = 1.2 Identities = 16/48 (33%), Positives = 25/48 (52%) Frame = +1 Query: 220 ASPCCRGSRPWLSIKSDKDKFQVNLDVQHFAPEEISVKTADGYIVVEG 363 A PC R +R L ++ K +VN D H PE+ ++ + D + V G Sbjct: 203 AKPCKRLTRSMLKVEGIKS--EVNADDDHVNPEKDAIGSEDNCVDVSG 248 >At1g78910.1 68414.m09199 pseudouridine synthase family protein low similarity to SP|P23851 Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate synthase) (Uracil hydrolyase) {Escherichia coli}; contains Pfam profile PF00849: RNA pseudouridylate synthase Length = 478 Score = 29.1 bits (62), Expect = 2.2 Identities = 23/59 (38%), Positives = 26/59 (44%), Gaps = 2/59 (3%) Frame = -2 Query: 460 TPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPR--CSRPPF*QRFLPGRNAGRPG 290 T I+ YS A I N A L P+ PR S PPF LPG+NAG G Sbjct: 14 TVLIRRYSRVAPPPSSVIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKNAGADG 72 >At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein contains Pfam profile: PF04615 Utp14 protein Length = 822 Score = 28.7 bits (61), Expect = 2.9 Identities = 17/54 (31%), Positives = 23/54 (42%) Frame = +1 Query: 316 EEISVKTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSD 477 +EI D Y EG EE+ ++ R + L E C E+VES D Sbjct: 48 DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVESEDDDD 101 >At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containing protein low similarity to fertility restorer [Petunia x hybrida] GI:22128587, post-transcriptional control of chloroplast gene expression CRP1 [Zea mays] GI:3289002; contains Pfam profile PF01535: PPR repeat Length = 991 Score = 27.5 bits (58), Expect = 6.6 Identities = 15/48 (31%), Positives = 20/48 (41%) Frame = -2 Query: 481 HHLKTAGTPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPRCSRP 338 HHL + ++ P S P S + H SS PRCS+P Sbjct: 26 HHLALSSKARVFPLSLPCNFSSRVSFKLQLHCAASSSSSVSPPRCSKP 73 >At1g34180.1 68414.m04239 no apical meristem (NAM) family protein contains Pfam PF02365: No apical meristem (NAM) domain; similar to NAM-like protein GI:8809651 from (Arabidopsis thaliana) Length = 564 Score = 27.5 bits (58), Expect = 6.6 Identities = 16/50 (32%), Positives = 24/50 (48%) Frame = +1 Query: 355 VEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSDGVLSVIAPR 504 +E E D G RQF +P+G +AE ++S L ++ I PR Sbjct: 229 IEKLLNEIPDAPGVNQRQFDEFVGVPQGNSAEVIQSTLLNNSSGEYIDPR 278 >At4g02400.1 68417.m00324 U3 ribonucleoprotein (Utp) family protein contains Pfam profile: PF04615 Utp14 protein Length = 858 Score = 27.1 bits (57), Expect = 8.7 Identities = 17/50 (34%), Positives = 22/50 (44%) Frame = +1 Query: 331 KTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSDG 480 + D Y EG EE+ ++ R Y LPE E+VES DG Sbjct: 52 ENVDLYEYEEGVPEEESKKNNRYDRVDNYDYELPEDFEDENVESDDDEDG 101 >At2g01960.1 68415.m00131 expressed protein Length = 260 Score = 27.1 bits (57), Expect = 8.7 Identities = 10/24 (41%), Positives = 15/24 (62%) Frame = +1 Query: 163 RFSQCCSRPPCEQRILPPVASPCC 234 RF + PP ++R+LP V + CC Sbjct: 147 RFVNSYALPPYDRRLLPSVKTGCC 170 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,928,107 Number of Sequences: 28952 Number of extensions: 283562 Number of successful extensions: 772 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 757 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 772 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1092379416 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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