Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV10952
(652 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 114 4e-26
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 114 4e-26
At2g33590.1 68415.m04117 cinnamoyl-CoA reductase family similar ... 32 0.38
At3g18640.1 68416.m02368 zinc finger protein-related contains si... 29 2.0
At2g33600.1 68415.m04118 cinnamoyl-CoA reductase family similar ... 29 3.5
At4g39480.1 68417.m05585 cytochrome P450 family protein contains... 28 4.7
At3g10790.1 68416.m01299 F-box family protein contains F-box dom... 28 4.7
At1g57750.1 68414.m06552 cytochrome P450, putative similar to cy... 28 4.7
At3g60300.1 68416.m06740 RWD domain-containing protein contains ... 28 6.2
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 114 bits (275), Expect = 4e-26
Identities = 52/74 (70%), Positives = 59/74 (79%)
Frame = +2
Query: 35 TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAGHQTSAESWGTGRAVAR 214
T Q LP V AP+RPD+VN VH +S NSRQPY VSK+AGHQTSAESWGTGRAV+R
Sbjct: 22 TDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKAGHQTSAESWGTGRAVSR 81
Query: 215 IPRVRGGGTHRSGR 256
IPRV GGGTHR+G+
Sbjct: 82 IPRVPGGGTHRAGQ 95
Score = 59.3 bits (137), Expect = 2e-09
Identities = 25/48 (52%), Positives = 36/48 (75%)
Frame = +3
Query: 366 SVAATGVPALVQARGHIIEKIPELPLVVADKVQEINKTKQAVIFLRRL 509
++AAT VPALV ARGH IE +PE+PLVV+D + + KT A+ L+++
Sbjct: 133 AIAATAVPALVMARGHKIENVPEMPLVVSDSAEAVEKTSAAIKVLKQI 180
Score = 55.2 bits (127), Expect = 4e-08
Identities = 21/22 (95%), Positives = 21/22 (95%)
Frame = +1
Query: 259 AFGNMCRGGRMFAPTKPWRRWH 324
AFGNMCRGGRMFAPTK WRRWH
Sbjct: 97 AFGNMCRGGRMFAPTKIWRRWH 118
Score = 46.4 bits (105), Expect = 2e-05
Identities = 21/36 (58%), Positives = 25/36 (69%)
Frame = +2
Query: 512 AWSDILKVYKSQRLRAGKGKMRNRRRIQRKGPLIIF 619
A+ D K S +R GKGKMRNRR I RKGPL++F
Sbjct: 182 AYDDAEKAKNSIGIRPGKGKMRNRRYISRKGPLVVF 217
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 114 bits (275), Expect = 4e-26
Identities = 52/74 (70%), Positives = 60/74 (81%)
Frame = +2
Query: 35 TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAGHQTSAESWGTGRAVAR 214
T Q + LP V AP+RPD+VN VH +S NSRQPY VSK+AGHQTSAESWGTGRAV+R
Sbjct: 21 TDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKAGHQTSAESWGTGRAVSR 80
Query: 215 IPRVRGGGTHRSGR 256
IPRV GGGTHR+G+
Sbjct: 81 IPRVPGGGTHRAGQ 94
Score = 59.3 bits (137), Expect = 2e-09
Identities = 25/48 (52%), Positives = 36/48 (75%)
Frame = +3
Query: 366 SVAATGVPALVQARGHIIEKIPELPLVVADKVQEINKTKQAVIFLRRL 509
++AAT VPALV ARGH IE +PE+PLVV+D + + KT A+ L+++
Sbjct: 132 AIAATAVPALVMARGHKIENVPEMPLVVSDSAEAVEKTSAAIKVLKQI 179
Score = 55.2 bits (127), Expect = 4e-08
Identities = 21/22 (95%), Positives = 21/22 (95%)
Frame = +1
Query: 259 AFGNMCRGGRMFAPTKPWRRWH 324
AFGNMCRGGRMFAPTK WRRWH
Sbjct: 96 AFGNMCRGGRMFAPTKIWRRWH 117
Score = 45.2 bits (102), Expect = 4e-05
Identities = 20/36 (55%), Positives = 25/36 (69%)
Frame = +2
Query: 512 AWSDILKVYKSQRLRAGKGKMRNRRRIQRKGPLIIF 619
A+ D K S +R GKGKMRNRR I RKGPL+++
Sbjct: 181 AYDDAEKAKNSIGIRPGKGKMRNRRYISRKGPLVVY 216
>At2g33590.1 68415.m04117 cinnamoyl-CoA reductase family similar to
cinnamoyl-CoA reductase from Pinus taeda [GI:17978649],
Eucalyptus gunnii [GI:2058311]
Length = 321
Score = 31.9 bits (69), Expect = 0.38
Identities = 22/89 (24%), Positives = 35/89 (39%)
Frame = -1
Query: 625 FVEDYEGSLTLDTTTVAHFTLTSTKTLRLVHLKDIRPCLRRLRKMTACLVLLISWTLSAT 446
F+ + L L H T+ + HLK + +L+ A L L +L +
Sbjct: 17 FLGSWVVDLLLSKDYFVHGTVRDPDNEKYAHLKKLEKAGDKLKLFKADL--LDYGSLQSA 74
Query: 445 TRGSSGIFSIMCPLA*TSAGTPVAATLPP 359
G SG+F + CP+ S P + P
Sbjct: 75 IAGCSGVFHVACPVPPASVPNPEVELIAP 103
>At3g18640.1 68416.m02368 zinc finger protein-related contains
similarity to zinc finger proteins (CCCH type)
Length = 676
Score = 29.5 bits (63), Expect = 2.0
Identities = 17/51 (33%), Positives = 26/51 (50%)
Frame = +3
Query: 291 VRPHEALAALAPSRQPPTAESGLGGSVAATGVPALVQARGHIIEKIPELPL 443
V+P++A P GLG S A GVPA+ ++ +E+I E+ L
Sbjct: 488 VQPNQATTQSNVVSSNPNQLWGLGMSTGAEGVPAVTASKISNVEEIQEVSL 538
>At2g33600.1 68415.m04118 cinnamoyl-CoA reductase family similar to
cinnamoyl-CoA reductase from Pinus taeda [GI:17978649],
Eucalyptus gunnii [GI:2058311]
Length = 321
Score = 28.7 bits (61), Expect = 3.5
Identities = 19/72 (26%), Positives = 30/72 (41%)
Frame = -1
Query: 574 HFTLTSTKTLRLVHLKDIRPCLRRLRKMTACLVLLISWTLSATTRGSSGIFSIMCPLA*T 395
H T+ + HLK + +L+ A L L +L + G SG+F + CP+
Sbjct: 34 HGTVRDPGNEKYAHLKKLDKAGDKLKLFKADL--LNYGSLQSAIAGCSGVFHVACPVPSA 91
Query: 394 SAGTPVAATLPP 359
S P + P
Sbjct: 92 SVPNPEVDLIAP 103
>At4g39480.1 68417.m05585 cytochrome P450 family protein contains
Pfam profile: PF00067 cytochrome P450
Length = 989
Score = 28.3 bits (60), Expect = 4.7
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +2
Query: 56 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 151
P+PF K+P +PD++ H + NSR +C+
Sbjct: 384 PVPFNHKSPAKPDVLPSGH-KVKANSRILFCL 414
Score = 27.5 bits (58), Expect = 8.2
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +2
Query: 56 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 151
P+PF K+P +PD++ H + NS+ +C+
Sbjct: 857 PVPFQHKSPTKPDVLPSGH-KVDANSKILFCL 887
>At3g10790.1 68416.m01299 F-box family protein contains F-box domain
Pfam:PF00646
Length = 319
Score = 28.3 bits (60), Expect = 4.7
Identities = 9/26 (34%), Positives = 12/26 (46%)
Frame = +2
Query: 446 CSRQSPGDQQDQTGCHLPEAPKAWSD 523
CSR+ P GC+ P+ W D
Sbjct: 99 CSREDPSSDHQTVGCYPDSYPRIWYD 124
>At1g57750.1 68414.m06552 cytochrome P450, putative similar to
cytochrome P450 GI:4688670 from [Catharanthus roseus]
Length = 497
Score = 28.3 bits (60), Expect = 4.7
Identities = 15/45 (33%), Positives = 25/45 (55%)
Frame = +2
Query: 17 HQEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 151
H SE+++ PLPF K+P +PD++ H + NS+ C+
Sbjct: 355 HAALSESMR-LYPPLPFNHKSPAKPDVLPSGH-KVDANSKIVICI 397
>At3g60300.1 68416.m06740 RWD domain-containing protein contains
weak similarity to RING finger protein 25 (RING finger
protein AO7) (Swiss-Prot:Q9QZR0) [Mus musculus]
Length = 366
Score = 27.9 bits (59), Expect = 6.2
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = +2
Query: 554 RAGKGKMRNRRRIQRKGPLIIFNKGSGSDSRLP 652
R G+G+ R R R + +GP + K + D R P
Sbjct: 321 RRGRGRWRGRGRSRGRGPTVNERKPNSQDPRKP 353
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,385,039
Number of Sequences: 28952
Number of extensions: 303587
Number of successful extensions: 918
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 862
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 917
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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