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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV10940
         (635 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |...   126   3e-30
SPAP8A3.13c |||Vid 24 family protein|Schizosaccharomyces pombe|c...    33   0.035
SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|...    27   3.0  
SPCC1620.09c |tfg1||transcription factor TFIIF complex alpha sub...    27   3.0  
SPBC530.05 |||transcription factor |Schizosaccharomyces pombe|ch...    26   5.2  
SPAC3F10.17 |||ribosome biogenesis protein Ltv1|Schizosaccharomy...    26   5.2  
SPCC1450.12 |||conserved fungal protein|Schizosaccharomyces pomb...    25   6.9  
SPCC736.03c |||phenylalanyl-tRNA synthetase|Schizosaccharomyces ...    25   6.9  
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    25   9.1  
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ...    25   9.1  

>SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 499

 Score =  126 bits (304), Expect = 3e-30
 Identities = 55/85 (64%), Positives = 66/85 (77%)
 Frame = +2

Query: 2   KLETDLTSDMLLSGSWKTLEFKPYNFDALGQPPECGHLHPLLKVRSEFREIFLEMGFTEM 181
           KL TDLT++M+ S SW++ +FK YNF A G PP  G LHPL+KVR EFR+ F E+GF EM
Sbjct: 186 KLNTDLTAEMITSRSWESAKFKSYNFAAEGIPPAGGCLHPLMKVREEFRKFFFELGFEEM 245

Query: 182 PTNKYIESSFWNFDALFQPQQHPAR 256
           PTN ++ES FWNFDALF PQQH AR
Sbjct: 246 PTNNFVESGFWNFDALFVPQQHSAR 270



 Score = 79.0 bits (186), Expect = 5e-16
 Identities = 40/75 (53%), Positives = 51/75 (68%), Gaps = 2/75 (2%)
 Frame = +1

Query: 250 SQDAHDTFFMSSPAATTEFP-MEYLERVKKVHSKGGYGSQ-GYRYDWKIEEAQKNLLRTH 423
           ++DA DTFF+  PA+T + P  EY+ RVK  H  GG     GYR  + +EE +K +LRTH
Sbjct: 269 ARDAQDTFFLKVPASTDKLPDPEYVARVKATHENGGETKGIGYRAPFSLEETRKLVLRTH 328

Query: 424 TTAVSARMLYRLAHN 468
           TTAVSA MLY+LA N
Sbjct: 329 TTAVSANMLYKLAQN 343



 Score = 64.9 bits (151), Expect = 9e-12
 Identities = 28/42 (66%), Positives = 34/42 (80%)
 Frame = +2

Query: 509 FRNETLDATHLAEFHQVEGVVADRGLGLADLITVLDAFFKRL 634
           FRNET+DATHLAEFHQVEGV+ DR + L DLI  L+ FF ++
Sbjct: 357 FRNETVDATHLAEFHQVEGVICDRNITLGDLIGFLEVFFGKM 398



 Score = 25.4 bits (53), Expect = 6.9
 Identities = 11/16 (68%), Positives = 12/16 (75%)
 Frame = +3

Query: 462 AQPEFTPQKYFSIDKV 509
           AQ  F P KYFSID+V
Sbjct: 341 AQNGFHPAKYFSIDRV 356


>SPAP8A3.13c |||Vid 24 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 547

 Score = 33.1 bits (72), Expect = 0.035
 Identities = 16/46 (34%), Positives = 24/46 (52%)
 Frame = +2

Query: 110 HLHPLLKVRSEFREIFLEMGFTEMPTNKYIESSFWNFDALFQPQQH 247
           H+ PL ++RSE  E    + FT  P +  + +   N D +F PQ H
Sbjct: 47  HMFPLARIRSELSEQDSSISFTHDPLHIPLPNPSNNNDNIFHPQVH 92


>SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 531

 Score = 26.6 bits (56), Expect = 3.0
 Identities = 12/22 (54%), Positives = 13/22 (59%)
 Frame = +2

Query: 509 FRNETLDATHLAEFHQVEGVVA 574
           FRNE +DATH  EF   E   A
Sbjct: 281 FRNEGIDATHNPEFTSCEAYCA 302


>SPCC1620.09c |tfg1||transcription factor TFIIF complex alpha
           subunit Tfg1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 490

 Score = 26.6 bits (56), Expect = 3.0
 Identities = 15/63 (23%), Positives = 29/63 (46%)
 Frame = +3

Query: 345 QRWIWLPGLQIRLEDRGGAEEPAADAHDGGQRQDAVQTRAQPEFTPQKYFSIDKVSATRP 524
           QR++ L    +  E     E+P A +HD    ++ +Q   + +   +K  S    +A++ 
Sbjct: 329 QRYLKLLEKNLAYESDEEDEDPYASSHDASSEEEVLQEEEELQKREEKLKSRFSANASKT 388

Query: 525 WTP 533
            TP
Sbjct: 389 NTP 391


>SPBC530.05 |||transcription factor |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 743

 Score = 25.8 bits (54), Expect = 5.2
 Identities = 21/85 (24%), Positives = 32/85 (37%)
 Frame = +3

Query: 249 QPGCSRHILYVLACCHHGVPHGIPGEGEEGAQQRWIWLPGLQIRLEDRGGAEEPAADAHD 428
           QP CS        C  HG+P       +    QR +++  L  RLE            +D
Sbjct: 25  QPACSN-------CVSHGIPCVFTARPKRRTGQRQMYIKSLVSRLEQMESTLRSVIPNYD 77

Query: 429 GGQRQDAVQTRAQPEFTPQKYFSID 503
             Q+ D +     P  TP+ Y  ++
Sbjct: 78  --QQPDII----HPSSTPKNYHDLN 96


>SPAC3F10.17 |||ribosome biogenesis protein Ltv1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 386

 Score = 25.8 bits (54), Expect = 5.2
 Identities = 12/35 (34%), Positives = 16/35 (45%)
 Frame = +3

Query: 384 EDRGGAEEPAADAHDGGQRQDAVQTRAQPEFTPQK 488
           ED    E PA       Q++  +Q R QP   PQ+
Sbjct: 94  EDATWVEAPATRKTQDKQKKQQIQLRDQPSILPQE 128


>SPCC1450.12 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 821

 Score = 25.4 bits (53), Expect = 6.9
 Identities = 21/90 (23%), Positives = 35/90 (38%)
 Frame = +1

Query: 247 PSQDAHDTFFMSSPAATTEFPMEYLERVKKVHSKGGYGSQGYRYDWKIEEAQKNLLRTHT 426
           P +D HDT+  SS  +T               S   +  +  R   ++ +  KN+L    
Sbjct: 233 PPKDDHDTYLNSSEDSTLSPLPSRSSDTNDPQSDSQHVLRQERMRIELRQYIKNILNDDE 292

Query: 427 TAVSARMLYRLAHNQSSLRRSTLASIRFPQ 516
             ++  +L  L  +  +L  S L  I F Q
Sbjct: 293 LHLTEEVLSFLTDDPVTLTASELTDINFRQ 322


>SPCC736.03c |||phenylalanyl-tRNA synthetase|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 429

 Score = 25.4 bits (53), Expect = 6.9
 Identities = 8/21 (38%), Positives = 15/21 (71%)
 Frame = +2

Query: 509 FRNETLDATHLAEFHQVEGVV 571
           +R + +D++H   FHQ+EG +
Sbjct: 142 YRRDEVDSSHYPIFHQMEGAL 162



 Score = 25.0 bits (52), Expect = 9.1
 Identities = 13/47 (27%), Positives = 21/47 (44%)
 Frame = +2

Query: 116 HPLLKVRSEFREIFLEMGFTEMPTNKYIESSFWNFDALFQPQQHPAR 256
           HP+  +R    + F +     +     I S   NFD+L  P+ H +R
Sbjct: 50  HPICSLRQLLEQQFQKFEMNNVQKESPIVSVETNFDSLGFPKTHVSR 96


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with
           EF hand and WH2 motif |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1794

 Score = 25.0 bits (52), Expect = 9.1
 Identities = 19/78 (24%), Positives = 32/78 (41%)
 Frame = +3

Query: 300 GVPHGIPGEGEEGAQQRWIWLPGLQIRLEDRGGAEEPAADAHDGGQRQDAVQTRAQPEFT 479
           G+   +PG  +  A QR     G+Q  +  R G ++P A    G Q     +T  QP+  
Sbjct: 574 GMQPQMPGMQQPMAPQR----TGMQPMMPQRTGMQQPMAPQRTGMQPMMPQRTGMQPQMP 629

Query: 480 PQKYFSIDKVSATRPWTP 533
             +     + +  +P  P
Sbjct: 630 GMQQPMAPQRTGMQPMMP 647


>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1044

 Score = 25.0 bits (52), Expect = 9.1
 Identities = 8/20 (40%), Positives = 15/20 (75%)
 Frame = -2

Query: 529 VQGLVAETLSMLKYFCGVNS 470
           +QG++A  LS++ Y+C + S
Sbjct: 543 IQGMIAVFLSLVYYYCPIES 562


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,539,058
Number of Sequences: 5004
Number of extensions: 52333
Number of successful extensions: 151
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 143
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 149
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 283719918
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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