BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10940 (635 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |... 126 3e-30 SPAP8A3.13c |||Vid 24 family protein|Schizosaccharomyces pombe|c... 33 0.035 SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|... 27 3.0 SPCC1620.09c |tfg1||transcription factor TFIIF complex alpha sub... 27 3.0 SPBC530.05 |||transcription factor |Schizosaccharomyces pombe|ch... 26 5.2 SPAC3F10.17 |||ribosome biogenesis protein Ltv1|Schizosaccharomy... 26 5.2 SPCC1450.12 |||conserved fungal protein|Schizosaccharomyces pomb... 25 6.9 SPCC736.03c |||phenylalanyl-tRNA synthetase|Schizosaccharomyces ... 25 6.9 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 25 9.1 SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 25 9.1 >SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 499 Score = 126 bits (304), Expect = 3e-30 Identities = 55/85 (64%), Positives = 66/85 (77%) Frame = +2 Query: 2 KLETDLTSDMLLSGSWKTLEFKPYNFDALGQPPECGHLHPLLKVRSEFREIFLEMGFTEM 181 KL TDLT++M+ S SW++ +FK YNF A G PP G LHPL+KVR EFR+ F E+GF EM Sbjct: 186 KLNTDLTAEMITSRSWESAKFKSYNFAAEGIPPAGGCLHPLMKVREEFRKFFFELGFEEM 245 Query: 182 PTNKYIESSFWNFDALFQPQQHPAR 256 PTN ++ES FWNFDALF PQQH AR Sbjct: 246 PTNNFVESGFWNFDALFVPQQHSAR 270 Score = 79.0 bits (186), Expect = 5e-16 Identities = 40/75 (53%), Positives = 51/75 (68%), Gaps = 2/75 (2%) Frame = +1 Query: 250 SQDAHDTFFMSSPAATTEFP-MEYLERVKKVHSKGGYGSQ-GYRYDWKIEEAQKNLLRTH 423 ++DA DTFF+ PA+T + P EY+ RVK H GG GYR + +EE +K +LRTH Sbjct: 269 ARDAQDTFFLKVPASTDKLPDPEYVARVKATHENGGETKGIGYRAPFSLEETRKLVLRTH 328 Query: 424 TTAVSARMLYRLAHN 468 TTAVSA MLY+LA N Sbjct: 329 TTAVSANMLYKLAQN 343 Score = 64.9 bits (151), Expect = 9e-12 Identities = 28/42 (66%), Positives = 34/42 (80%) Frame = +2 Query: 509 FRNETLDATHLAEFHQVEGVVADRGLGLADLITVLDAFFKRL 634 FRNET+DATHLAEFHQVEGV+ DR + L DLI L+ FF ++ Sbjct: 357 FRNETVDATHLAEFHQVEGVICDRNITLGDLIGFLEVFFGKM 398 Score = 25.4 bits (53), Expect = 6.9 Identities = 11/16 (68%), Positives = 12/16 (75%) Frame = +3 Query: 462 AQPEFTPQKYFSIDKV 509 AQ F P KYFSID+V Sbjct: 341 AQNGFHPAKYFSIDRV 356 >SPAP8A3.13c |||Vid 24 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 547 Score = 33.1 bits (72), Expect = 0.035 Identities = 16/46 (34%), Positives = 24/46 (52%) Frame = +2 Query: 110 HLHPLLKVRSEFREIFLEMGFTEMPTNKYIESSFWNFDALFQPQQH 247 H+ PL ++RSE E + FT P + + + N D +F PQ H Sbjct: 47 HMFPLARIRSELSEQDSSISFTHDPLHIPLPNPSNNNDNIFHPQVH 92 >SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|||Manual Length = 531 Score = 26.6 bits (56), Expect = 3.0 Identities = 12/22 (54%), Positives = 13/22 (59%) Frame = +2 Query: 509 FRNETLDATHLAEFHQVEGVVA 574 FRNE +DATH EF E A Sbjct: 281 FRNEGIDATHNPEFTSCEAYCA 302 >SPCC1620.09c |tfg1||transcription factor TFIIF complex alpha subunit Tfg1|Schizosaccharomyces pombe|chr 3|||Manual Length = 490 Score = 26.6 bits (56), Expect = 3.0 Identities = 15/63 (23%), Positives = 29/63 (46%) Frame = +3 Query: 345 QRWIWLPGLQIRLEDRGGAEEPAADAHDGGQRQDAVQTRAQPEFTPQKYFSIDKVSATRP 524 QR++ L + E E+P A +HD ++ +Q + + +K S +A++ Sbjct: 329 QRYLKLLEKNLAYESDEEDEDPYASSHDASSEEEVLQEEEELQKREEKLKSRFSANASKT 388 Query: 525 WTP 533 TP Sbjct: 389 NTP 391 >SPBC530.05 |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 743 Score = 25.8 bits (54), Expect = 5.2 Identities = 21/85 (24%), Positives = 32/85 (37%) Frame = +3 Query: 249 QPGCSRHILYVLACCHHGVPHGIPGEGEEGAQQRWIWLPGLQIRLEDRGGAEEPAADAHD 428 QP CS C HG+P + QR +++ L RLE +D Sbjct: 25 QPACSN-------CVSHGIPCVFTARPKRRTGQRQMYIKSLVSRLEQMESTLRSVIPNYD 77 Query: 429 GGQRQDAVQTRAQPEFTPQKYFSID 503 Q+ D + P TP+ Y ++ Sbjct: 78 --QQPDII----HPSSTPKNYHDLN 96 >SPAC3F10.17 |||ribosome biogenesis protein Ltv1|Schizosaccharomyces pombe|chr 1|||Manual Length = 386 Score = 25.8 bits (54), Expect = 5.2 Identities = 12/35 (34%), Positives = 16/35 (45%) Frame = +3 Query: 384 EDRGGAEEPAADAHDGGQRQDAVQTRAQPEFTPQK 488 ED E PA Q++ +Q R QP PQ+ Sbjct: 94 EDATWVEAPATRKTQDKQKKQQIQLRDQPSILPQE 128 >SPCC1450.12 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 821 Score = 25.4 bits (53), Expect = 6.9 Identities = 21/90 (23%), Positives = 35/90 (38%) Frame = +1 Query: 247 PSQDAHDTFFMSSPAATTEFPMEYLERVKKVHSKGGYGSQGYRYDWKIEEAQKNLLRTHT 426 P +D HDT+ SS +T S + + R ++ + KN+L Sbjct: 233 PPKDDHDTYLNSSEDSTLSPLPSRSSDTNDPQSDSQHVLRQERMRIELRQYIKNILNDDE 292 Query: 427 TAVSARMLYRLAHNQSSLRRSTLASIRFPQ 516 ++ +L L + +L S L I F Q Sbjct: 293 LHLTEEVLSFLTDDPVTLTASELTDINFRQ 322 >SPCC736.03c |||phenylalanyl-tRNA synthetase|Schizosaccharomyces pombe|chr 3|||Manual Length = 429 Score = 25.4 bits (53), Expect = 6.9 Identities = 8/21 (38%), Positives = 15/21 (71%) Frame = +2 Query: 509 FRNETLDATHLAEFHQVEGVV 571 +R + +D++H FHQ+EG + Sbjct: 142 YRRDEVDSSHYPIFHQMEGAL 162 Score = 25.0 bits (52), Expect = 9.1 Identities = 13/47 (27%), Positives = 21/47 (44%) Frame = +2 Query: 116 HPLLKVRSEFREIFLEMGFTEMPTNKYIESSFWNFDALFQPQQHPAR 256 HP+ +R + F + + I S NFD+L P+ H +R Sbjct: 50 HPICSLRQLLEQQFQKFEMNNVQKESPIVSVETNFDSLGFPKTHVSR 96 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 25.0 bits (52), Expect = 9.1 Identities = 19/78 (24%), Positives = 32/78 (41%) Frame = +3 Query: 300 GVPHGIPGEGEEGAQQRWIWLPGLQIRLEDRGGAEEPAADAHDGGQRQDAVQTRAQPEFT 479 G+ +PG + A QR G+Q + R G ++P A G Q +T QP+ Sbjct: 574 GMQPQMPGMQQPMAPQR----TGMQPMMPQRTGMQQPMAPQRTGMQPMMPQRTGMQPQMP 629 Query: 480 PQKYFSIDKVSATRPWTP 533 + + + +P P Sbjct: 630 GMQQPMAPQRTGMQPMMP 647 >SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1044 Score = 25.0 bits (52), Expect = 9.1 Identities = 8/20 (40%), Positives = 15/20 (75%) Frame = -2 Query: 529 VQGLVAETLSMLKYFCGVNS 470 +QG++A LS++ Y+C + S Sbjct: 543 IQGMIAVFLSLVYYYCPIES 562 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,539,058 Number of Sequences: 5004 Number of extensions: 52333 Number of successful extensions: 151 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 143 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 149 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 283719918 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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