Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV10884
         (685 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   159   5e-40
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   156   3e-39
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    85   7e-18
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    52   1e-07
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual    28   1.4  
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1...    27   2.5  
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch...    27   3.3  
SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||...    26   4.4  
SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   5.8  
SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|c...    26   5.8  
SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po...    25   7.7  
SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces...    25   7.7  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  159 bits (385), Expect = 5e-40
 Identities = 70/89 (78%), Positives = 78/89 (87%)
 Frame = +3

Query: 255 ITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFE 434
           ITASLRF+G+LNVDL EFQTNLVPYPRIHFPLVTYAP++SA KA+HE  SV EITN CFE
Sbjct: 238 ITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFE 297

Query: 435 PANQMVKCDPRHGKYMACCMLYRGESYPR 521
           P NQMVKCDPR G+YMA C+LYRG+  PR
Sbjct: 298 PYNQMVKCDPRAGRYMATCLLYRGDVIPR 326



 Score =  134 bits (325), Expect = 8e-33
 Identities = 58/77 (75%), Positives = 69/77 (89%)
 Frame = +1

Query: 19  LNYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLD 198
           + Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLD
Sbjct: 159 MEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLD 218

Query: 199 IERPTYTNLNRLIGQIV 249
           IERP+Y NLNRLI Q+V
Sbjct: 219 IERPSYENLNRLIAQVV 235



 Score = 69.3 bits (162), Expect = 5e-13
 Identities = 28/38 (73%), Positives = 33/38 (86%)
 Frame = +2

Query: 509 VVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPP 622
           V+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI  +PP
Sbjct: 323 VIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPP 360



 Score = 31.9 bits (69), Expect = 0.089
 Identities = 13/17 (76%), Positives = 15/17 (88%)
 Frame = +1

Query: 634 GGDLAKVQRAVCMLSNT 684
           G ++AKV RAVCMLSNT
Sbjct: 365 GSEIAKVDRAVCMLSNT 381


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  156 bits (378), Expect = 3e-39
 Identities = 69/89 (77%), Positives = 77/89 (86%)
 Frame = +3

Query: 255 ITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFE 434
           ITASLRF G+LNVDL EFQTNLVPYPRIHFPLVTY+P++SA KA+HE  SV EITN CFE
Sbjct: 242 ITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFE 301

Query: 435 PANQMVKCDPRHGKYMACCMLYRGESYPR 521
           P NQMVKCDPR G+YMA C+LYRG+  PR
Sbjct: 302 PYNQMVKCDPRTGRYMATCLLYRGDVIPR 330



 Score =  141 bits (342), Expect = 7e-35
 Identities = 61/77 (79%), Positives = 70/77 (90%)
 Frame = +1

Query: 19  LNYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLD 198
           + YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLD
Sbjct: 163 MEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLD 222

Query: 199 IERPTYTNLNRLIGQIV 249
           IERPTY NLNRLI Q+V
Sbjct: 223 IERPTYENLNRLIAQVV 239



 Score = 74.1 bits (174), Expect = 2e-14
 Identities = 29/42 (69%), Positives = 37/42 (88%)
 Frame = +2

Query: 509 VVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVP 634
           V+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP  VP
Sbjct: 327 VIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVP 368



 Score = 33.5 bits (73), Expect = 0.029
 Identities = 14/18 (77%), Positives = 15/18 (83%)
 Frame = +1

Query: 631 PGGDLAKVQRAVCMLSNT 684
           PG  +AKV RAVCMLSNT
Sbjct: 368 PGSGIAKVNRAVCMLSNT 385


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 85.4 bits (202), Expect = 7e-18
 Identities = 36/85 (42%), Positives = 54/85 (63%)
 Frame = +3

Query: 255 ITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFE 434
           +T S RF G LN DL +   N+VP+PR+HF +V +AP+ +   +  + +SV E+T   F+
Sbjct: 236 VTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFD 295

Query: 435 PANQMVKCDPRHGKYMACCMLYRGE 509
             N MV  DPRHG+Y+    L+RG+
Sbjct: 296 ANNMMVAADPRHGRYLTVAALFRGK 320



 Score = 67.7 bits (158), Expect = 1e-12
 Identities = 30/75 (40%), Positives = 46/75 (61%)
 Frame = +1

Query: 25  YGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIE 204
           Y  +    F++ PAP+ S  VVEPYN+ L+ H  +E+SD  F +DNEA+  I    L I+
Sbjct: 159 YPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIK 218

Query: 205 RPTYTNLNRLIGQIV 249
            P+Y +LN L+  ++
Sbjct: 219 SPSYDDLNHLVSAVM 233



 Score = 28.3 bits (60), Expect = 1.1
 Identities = 11/35 (31%), Positives = 19/35 (54%)
 Frame = +2

Query: 518 KDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPP 622
           K+V+  I +++TK +  FV+W P      +   PP
Sbjct: 324 KEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPP 358


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 51.6 bits (118), Expect = 1e-07
 Identities = 31/108 (28%), Positives = 50/108 (46%), Gaps = 4/108 (3%)
 Frame = +3

Query: 207 PNLHQPESSHRTDCLLITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVIS---A 377
           P  HQ      T     T +LR+ G +N DL     +L+P PR HF L +Y P  +    
Sbjct: 223 PTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVE 282

Query: 378 EKAYHEQLSVAEITNACFEPANQMVKCDP-RHGKYMACCMLYRGESYP 518
           E     + +V ++      P NQMV  +P +   +++   + +GE+ P
Sbjct: 283 EAKAIRKTTVLDVMRRLLLPKNQMVSVNPSKKSCFISILDIIQGEADP 330



 Score = 48.0 bits (109), Expect = 1e-06
 Identities = 24/76 (31%), Positives = 41/76 (53%), Gaps = 1/76 (1%)
 Frame = +1

Query: 25  YGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDI 201
           Y KK    ++++P  Q VS  VV+PYNS+L       ++D   ++DN A+  I    L  
Sbjct: 161 YPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHT 220

Query: 202 ERPTYTNLNRLIGQIV 249
           + PT+   N+L+  ++
Sbjct: 221 QNPTFHQQNQLVSTVM 236



 Score = 29.5 bits (63), Expect = 0.47
 Identities = 10/38 (26%), Positives = 21/38 (55%)
 Frame = +2

Query: 515 PKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTV 628
           P DV+ ++  I+ +R   F+ W P   +V ++ + P +
Sbjct: 330 PADVHKSLLRIRERRYASFIPWGPASIQVALSKKSPYI 367


>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1611

 Score = 27.9 bits (59), Expect = 1.4
 Identities = 17/49 (34%), Positives = 26/49 (53%)
 Frame = +1

Query: 301  PSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTR 447
            P+++      P S +    T +SS+P  P  N+ PSP S + AS+ P R
Sbjct: 1232 PTAKAPPVPAPSSEAPSVSTPRSSVPS-PHSNASPSPTSSSMASAAPAR 1279


>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 857

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 13/37 (35%), Positives = 18/37 (48%)
 Frame = +3

Query: 516 PRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPP 626
           P +  RP++P  P  LS     V PV+  V +   PP
Sbjct: 557 PSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPP 593


>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 628

 Score = 26.6 bits (56), Expect = 3.3
 Identities = 15/35 (42%), Positives = 22/35 (62%), Gaps = 3/35 (8%)
 Frame = +3

Query: 537 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHPPWS 632
           +LP KPS+   +++S   V+P S   STTS+P  S
Sbjct: 5   TLPPKPSISPSIASSFPTVKPFSSQNSTTSNPELS 39


>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1158

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 11/28 (39%), Positives = 17/28 (60%)
 Frame = +1

Query: 364 QSSLPRRPTMNSFPSPRSQTHASSPPTR 447
           + SLPRRP+     +P S T ++ PP +
Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPPVK 765


>SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 751

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 16/47 (34%), Positives = 26/47 (55%)
 Frame = +1

Query: 298 SPSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSP 438
           SPS  LT  L   S++  S  + + + R+ T +S  SP+ Q+  S+P
Sbjct: 619 SPSKMLT-TLRNNSSTFPSLRKNAMIARKSTADSLSSPKRQSVPSTP 664


>SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 474

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 16/51 (31%), Positives = 31/51 (60%)
 Frame = +1

Query: 325 LTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMAS 477
           LTP + S +S +  +S  +R ++N+ PS  + +H+SS  T   +  PV+++
Sbjct: 130 LTPKNPSLFSSSNAAS--QRGSLNTAPSNFAYSHSSSLQTSASSRPPVLSN 178


>SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 665

 Score = 25.4 bits (53), Expect = 7.7
 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%)
 Frame = +3

Query: 534 PSLPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 629
           P  PS+P+++SN ST  G+Q V   V   +   W
Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564


>SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 476

 Score = 25.4 bits (53), Expect = 7.7
 Identities = 15/40 (37%), Positives = 17/40 (42%)
 Frame = +3

Query: 507 ESYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPP 626
           +  PR      LP K  VL    T +  VSR   TTS  P
Sbjct: 290 DKIPRPLLNSKLPRKTLVLDLDETLIHSVSRGSRTTSGQP 329


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,037,704
Number of Sequences: 5004
Number of extensions: 67179
Number of successful extensions: 251
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 224
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 251
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)