Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV10880X
(353 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g04620.2 68418.m00464 aminotransferase class I and II family ... 85 2e-17
At5g04620.1 68418.m00465 aminotransferase class I and II family ... 85 2e-17
At3g48790.1 68416.m05328 serine C-palmitoyltransferase, putative... 53 5e-08
At5g23670.1 68418.m02775 serine C-palmitoyltransferase (LCB2) id... 53 6e-08
At3g48780.1 68416.m05327 serine C-palmitoyltransferase, putative... 51 2e-07
At4g36480.1 68417.m05180 aminotransferase class I and II family ... 41 2e-04
At5g56980.1 68418.m07112 expressed protein non-consensus CG dono... 30 0.39
At1g17520.1 68414.m02153 DNA-binding protein, putative contains ... 30 0.39
At5g27920.1 68418.m03354 F-box family protein contains similarit... 29 1.2
At1g22730.1 68414.m02840 MA3 domain-containing protein contains ... 29 1.2
At5g22620.1 68418.m02643 phosphoglycerate/bisphosphoglycerate mu... 27 3.6
At5g17560.1 68418.m02060 BolA-like family protein contains Pfam ... 27 4.7
At1g26540.1 68414.m03234 agenet domain-containing protein contai... 27 4.7
At5g42940.1 68418.m05235 zinc finger (C3HC4-type RING finger) fa... 26 6.3
At5g50850.1 68418.m06300 pyruvate dehydrogenase E1 component bet... 26 8.3
At2g31480.1 68415.m03845 expressed protein contains Pfam profile... 26 8.3
At2g27430.1 68415.m03315 expressed protein 26 8.3
At1g20400.1 68414.m02544 myosin heavy chain-related 26 8.3
>At5g04620.2 68418.m00464 aminotransferase class I and II family
protein similar to 8-amino-7-oxononanoate synthase,
Bacillus sphaericus, PIR:JQ0512 [SP|P22806], Bacillus
subtilis [SP|P53556]; contains Pfam protile PF00155
aminotransferase, classes I and II
Length = 476
Score = 84.6 bits (200), Expect = 2e-17
Identities = 44/91 (48%), Positives = 59/91 (64%), Gaps = 4/91 (4%)
Frame = +1
Query: 4 SDALNHASIIDGIRLCKAQK----YRYPHRDLTELEHLLAHSDARIKLIVTDGVFSMDGT 171
SDALNHASIIDG+RL + Q + Y H D+ L LL++ + K++VTD +FSMDG
Sbjct: 205 SDALNHASIIDGVRLAERQGNVEVFVYRHCDMYHLNSLLSNCKMKRKVVVTDSLFSMDGD 264
Query: 172 VAPIKGLRDLADKYRALLVVDDSHATGFSGK 264
AP++ L L KY LLV+DD+H T G+
Sbjct: 265 FAPMEELSQLRKKYGFLLVIDDAHGTFVCGE 295
>At5g04620.1 68418.m00465 aminotransferase class I and II family
protein similar to 8-amino-7-oxononanoate synthase,
Bacillus sphaericus, PIR:JQ0512 [SP|P22806], Bacillus
subtilis [SP|P53556]; contains Pfam protile PF00155
aminotransferase, classes I and II
Length = 343
Score = 84.6 bits (200), Expect = 2e-17
Identities = 44/91 (48%), Positives = 59/91 (64%), Gaps = 4/91 (4%)
Frame = +1
Query: 4 SDALNHASIIDGIRLCKAQK----YRYPHRDLTELEHLLAHSDARIKLIVTDGVFSMDGT 171
SDALNHASIIDG+RL + Q + Y H D+ L LL++ + K++VTD +FSMDG
Sbjct: 72 SDALNHASIIDGVRLAERQGNVEVFVYRHCDMYHLNSLLSNCKMKRKVVVTDSLFSMDGD 131
Query: 172 VAPIKGLRDLADKYRALLVVDDSHATGFSGK 264
AP++ L L KY LLV+DD+H T G+
Sbjct: 132 FAPMEELSQLRKKYGFLLVIDDAHGTFVCGE 162
>At3g48790.1 68416.m05328 serine C-palmitoyltransferase, putative
similar to serine palmitoyltransferase from Solanum
tuberosum [GI:4995890], Homo sapiens [SP|O15270], Mus
musculus [SP|P97363]
Length = 350
Score = 53.2 bits (122), Expect = 5e-08
Identities = 29/93 (31%), Positives = 52/93 (55%), Gaps = 5/93 (5%)
Frame = +1
Query: 1 ISDALNHASIIDGIRLCKAQKYRYPHRDLTELEHLL-----AHSDARIKLIVTDGVFSMD 165
ISD+LNH SII+G R A + H L E H++ H + ++V +G++SM+
Sbjct: 53 ISDSLNHTSIINGARGSGATIRVFQHNILKE--HIIEGQPRTHRPWKKIIVVVEGIYSME 110
Query: 166 GTVAPIKGLRDLADKYRALLVVDDSHATGFSGK 264
G + + + + +Y+A + +D++H+ G GK
Sbjct: 111 GEICDLPEIVSVCSEYKAYVYLDEAHSIGAIGK 143
>At5g23670.1 68418.m02775 serine C-palmitoyltransferase (LCB2)
identical to serine palmitoyltransferase [Arabidopsis
thaliana] GI:9309380; similar to serine
palmitoyltransferase from Solanum tuberosum
[GI:4995890], Homo sapiens [SP|O15270], Mus musculus
[SP|P97363]; contains Pfam profile PF00155:
aminotransferase, classes I and II
Length = 489
Score = 52.8 bits (121), Expect = 6e-08
Identities = 29/103 (28%), Positives = 55/103 (53%), Gaps = 10/103 (9%)
Frame = +1
Query: 1 ISDALNHASIIDGIRLCKAQKYRYPHRDLTELEHLL----------AHSDARIKLIVTDG 150
ISD+LNH+SI++G R A + H + LE +L H + ++V +G
Sbjct: 189 ISDSLNHSSIVNGARGSGATIRVFQHNTPSHLERVLREQIAEGQPRTHRPWKKIIVVVEG 248
Query: 151 VFSMDGTVAPIKGLRDLADKYRALLVVDDSHATGFSGKLAEAL 279
++SM+G + + + + KY+A + +D++H+ G GK + +
Sbjct: 249 IYSMEGEICHLPEVVAICKKYKAYVYLDEAHSIGAIGKTGKGI 291
>At3g48780.1 68416.m05327 serine C-palmitoyltransferase, putative
similar to serine palmitoyltransferase from Solanum
tuberosum [GI:4995890], Homo sapiens [SP|O15270], Mus
musculus [SP|P97363]
Length = 489
Score = 51.2 bits (117), Expect = 2e-07
Identities = 29/98 (29%), Positives = 51/98 (52%), Gaps = 10/98 (10%)
Frame = +1
Query: 1 ISDALNHASIIDGIRLCKAQKYRYPHRDLTELEHLL----------AHSDARIKLIVTDG 150
ISD+LNH SI++G R A + H LE +L H + ++V +G
Sbjct: 189 ISDSLNHTSIVNGARGSGATIRVFQHNTPGHLEKVLKEQIAEGQPRTHRPWKKIIVVVEG 248
Query: 151 VFSMDGTVAPIKGLRDLADKYRALLVVDDSHATGFSGK 264
++SM+G + + + + KY+A + +D++H+ G GK
Sbjct: 249 IYSMEGEICHLPEIVSICKKYKAYVYLDEAHSIGAIGK 286
>At4g36480.1 68417.m05180 aminotransferase class I and II family
protein similar to Serine palmitoyltransferase 1 (EC
2.3.1.50) from Homo sapiens [SP|O15269], Mus musculus
[SP|O35704], Cricetulus griseus [SP|O54695]
Length = 482
Score = 41.1 bits (92), Expect = 2e-04
Identities = 25/100 (25%), Positives = 48/100 (48%), Gaps = 7/100 (7%)
Frame = +1
Query: 1 ISDALNHASIIDGIRLCKAQKYRYPHRDLTELEHLLAHSDARIK-------LIVTDGVFS 159
++D H I +G++L ++ + H D+ L L + K IV + V+
Sbjct: 197 VADEGVHWGIQNGLQLSRSTIVYFKHNDMESLRITLEKIMTKYKRSKNLRRYIVAEAVYQ 256
Query: 160 MDGTVAPIKGLRDLADKYRALLVVDDSHATGFSGKLAEAL 279
G +AP+ + L +KYR +++D+S++ G G+ L
Sbjct: 257 NSGQIAPLDEIVKLKEKYRFRVILDESNSFGVLGRSGRGL 296
>At5g56980.1 68418.m07112 expressed protein non-consensus CG donor
splice site at exon 1, GA donor splice site at exon 3
Length = 379
Score = 30.3 bits (65), Expect = 0.39
Identities = 20/66 (30%), Positives = 34/66 (51%)
Frame = +1
Query: 25 SIIDGIRLCKAQKYRYPHRDLTELEHLLAHSDARIKLIVTDGVFSMDGTVAPIKGLRDLA 204
SIID ++ Y++PH + TEL + AH D +I +D D AP++ L
Sbjct: 61 SIIDRVKSINFHLYKFPHPE-TELFSMTAHHD----IIGSDLHVYPDPNPAPLQRAPSLL 115
Query: 205 DKYRAL 222
D+ +++
Sbjct: 116 DRVKSI 121
>At1g17520.1 68414.m02153 DNA-binding protein, putative contains
similarity to DNA-binding protein PcMYB1 [Petroselinum
crispum] gi|2224899|gb|AAB61699
Length = 296
Score = 30.3 bits (65), Expect = 0.39
Identities = 31/113 (27%), Positives = 49/113 (43%)
Frame = +1
Query: 1 ISDALNHASIIDGIRLCKAQKYRYPHRDLTELEHLLAHSDARIKLIVTDGVFSMDGTVAP 180
+ D + S+ GI+ K K R P HL A + A I G S T+ P
Sbjct: 49 LKDKWRNLSVAPGIQGSK-DKIRTPKIKAAAF-HLAAAAAAAIVTPTHSGHSSPVATL-P 105
Query: 181 IKGLRDLADKYRALLVVDDSHATGFSGKLAEALKSIAVLWGRRISSVRPWAKQ 339
G DL+ +VVD +A + G + EAL ++ G +S++ + +Q
Sbjct: 106 RSGSSDLSIDDSFNIVVDPKNAPRYDGMIFEALSNLTDANGSDVSAIFNFIEQ 158
>At5g27920.1 68418.m03354 F-box family protein contains similarity
to leucine-rich repeats containing F-box protein FBL3
GI:5919219 from [Homo sapiens]
Length = 642
Score = 28.7 bits (61), Expect = 1.2
Identities = 21/53 (39%), Positives = 26/53 (49%), Gaps = 3/53 (5%)
Frame = +1
Query: 136 IVTDGVFSMD---GTVAPIKGLRDLADKYRALLVVDDSHATGFSGKLAEALKS 285
I T G+ S++ T +GL LA AL VD SH GF + A AL S
Sbjct: 92 ISTLGIKSLNLSRSTAVRARGLETLARMCHALERVDVSHCWGFGDREAAALSS 144
>At1g22730.1 68414.m02840 MA3 domain-containing protein contains
Pfam profile PF02847: MA3 domain; low similarity to
programmed cell death 4 protein [Gallus gallus]
GI:12958564
Length = 693
Score = 28.7 bits (61), Expect = 1.2
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = +2
Query: 281 RVLRCYGGGGYHLFDPGQS 337
R+LRC+GGGG PG +
Sbjct: 539 RILRCWGGGGIETNSPGST 557
>At5g22620.1 68418.m02643 phosphoglycerate/bisphosphoglycerate
mutase family protein weak similarity to SP|P15259
Phosphoglycerate mutase, muscle form (EC 5.4.2.1 {Homo
sapiens}; contains Pfam profile PF00300:
phosphoglycerate mutase family
Length = 482
Score = 27.1 bits (57), Expect = 3.6
Identities = 18/55 (32%), Positives = 28/55 (50%)
Frame = +1
Query: 157 SMDGTVAPIKGLRDLADKYRALLVVDDSHATGFSGKLAEALKSIAVLWGRRISSV 321
S D +V KG A+ R +L+ DDS F+ L + K+ ++WG R S +
Sbjct: 69 SSDFSVLTKKG-ESQAEISRQMLI-DDSFDVCFTSPLKRSKKTAEIIWGSRESEM 121
>At5g17560.1 68418.m02060 BolA-like family protein contains Pfam
profile: PF01722 BolA-like protein
Length = 177
Score = 26.6 bits (56), Expect = 4.7
Identities = 15/45 (33%), Positives = 21/45 (46%)
Frame = -1
Query: 254 KPVAWLSSTTNKALYLSARSLRPFIGATVPSIENTPSVTISLILA 120
+P LSST L S RPF + P + T S +S +L+
Sbjct: 10 RPYVLLSSTRASLYRLFQSSKRPFASFSAPLVRFTNSRCVSAVLS 54
>At1g26540.1 68414.m03234 agenet domain-containing protein contains
Pfam PF05641: Agenet domain
Length = 695
Score = 26.6 bits (56), Expect = 4.7
Identities = 16/41 (39%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
Frame = +1
Query: 67 RYPHR-DLTELEHLLAHSDARIKLIVTDGVFSMDGTVAPIK 186
RY R + T+ E + HSD R ++ DGV+ D PIK
Sbjct: 253 RYTVRLEATQQESTIRHSDLRPFMVWEDGVWYNDLKQKPIK 293
>At5g42940.1 68418.m05235 zinc finger (C3HC4-type RING finger)
family protein similar to Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 691
Score = 26.2 bits (55), Expect = 6.3
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = +2
Query: 305 GGYHLFDPGQSSEWS 349
GGYH F G+SS W+
Sbjct: 196 GGYHDFQRGESSSWT 210
>At5g50850.1 68418.m06300 pyruvate dehydrogenase E1 component beta
subunit, mitochondrial / PDHE1-B (PDH2) identical to
SP|Q38799 Pyruvate dehydrogenase E1 component beta
subunit, mitochondrial precursor (EC 1.2.4.1) (PDHE1-B)
{Arabidopsis thaliana}
Length = 363
Score = 25.8 bits (54), Expect = 8.3
Identities = 26/98 (26%), Positives = 41/98 (41%), Gaps = 5/98 (5%)
Frame = -1
Query: 347 STHCFAQGRTDDIRRPHN-TAILFSASASFPEKPVAWLSSTTNKALYLSARSLRPFIGAT 171
S+ C G+ R + T + FS F K L+ A ++ RS+RP AT
Sbjct: 222 SSFCLPIGKAKIEREGKDVTIVTFSKMVGFALKAAEKLAEEGISAEVINLRSIRPLDRAT 281
Query: 170 V-PSIENTPS-VTI--SLILASLCANKCSNSVKSL*GY 69
+ S+ T VT+ +CA C++ V+ Y
Sbjct: 282 INASVRKTSRLVTVEEGFPQHGVCAEICASVVEESFSY 319
>At2g31480.1 68415.m03845 expressed protein contains Pfam profile
PF03754: Domain of unknown function (DUF313)
Length = 472
Score = 25.8 bits (54), Expect = 8.3
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = -1
Query: 266 SFPEKPVAWLSSTTNKALYLSARS 195
S EK V+WLSS N +L L+ S
Sbjct: 130 SVDEKIVSWLSSVANSSLSLNQES 153
>At2g27430.1 68415.m03315 expressed protein
Length = 438
Score = 25.8 bits (54), Expect = 8.3
Identities = 19/68 (27%), Positives = 36/68 (52%)
Frame = -1
Query: 302 PHNTAILFSASASFPEKPVAWLSSTTNKALYLSARSLRPFIGATVPSIENTPSVTISLIL 123
P N +L ++ + + LSS TN L +++ + PF+ T+ S ++T T + L
Sbjct: 167 PKNVEVLDQSTRHAFAELLLSLSSLTNTQLPVASSQILPFLMDTMNS-DSTDMKTKEICL 225
Query: 122 ASLCANKC 99
A++ +N C
Sbjct: 226 ATI-SNLC 232
>At1g20400.1 68414.m02544 myosin heavy chain-related
Length = 944
Score = 25.8 bits (54), Expect = 8.3
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = +1
Query: 193 RDLADKYRALLVVDDSHATGFSGKLAEALKSIAVLWGRRISS 318
R AD++RA + DD G+S + + S+ RRISS
Sbjct: 166 RSGADRFRAESLDDDLVEAGYSSSGSSSRTSVVAELRRRISS 207
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,795,842
Number of Sequences: 28952
Number of extensions: 139243
Number of successful extensions: 417
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 406
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 412
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 449370720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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