Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV10879X
         (433 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   134   5e-33
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   122   2e-29
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    74   1e-14
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    59   2e-10
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   2.8  
SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|c...    25   5.0  
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    24   8.7  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  134 bits (324), Expect = 5e-33
 Identities = 57/85 (67%), Positives = 70/85 (82%)
 Frame = +3

Query: 3   GIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFH 182
           GIQP+G M  +      D  F+TFFSETG GK+VPR+++VDLEP V+D+VRTG YR LFH
Sbjct: 29  GIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFH 88

Query: 183 PQQLITGKEDAANNYARGHYTIGKK 257
           P+QLITGKEDA+NNYARGHYT+GK+
Sbjct: 89  PEQLITGKEDASNNYARGHYTVGKE 113



 Score = 72.9 bits (171), Expect = 2e-14
 Identities = 32/61 (52%), Positives = 44/61 (72%)
 Frame = +2

Query: 251 KEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFVI 430
           KE+VD V D+IR++AD C+GLQGFL+FH            LL+ERL+++Y KKSKL+F +
Sbjct: 112 KELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSV 171

Query: 431 Y 433
           Y
Sbjct: 172 Y 172


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  122 bits (295), Expect = 2e-29
 Identities = 56/90 (62%), Positives = 70/90 (77%), Gaps = 5/90 (5%)
 Frame = +3

Query: 3   GIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTY 167
           GI PDG  PT+     K     +D F TFFSETG GK VPR+++VDLEP V+D+VRTG Y
Sbjct: 29  GIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPY 87

Query: 168 RQLFHPQQLITGKEDAANNYARGHYTIGKK 257
           + LFHP+Q++TGKEDA+NNYARGHYT+GK+
Sbjct: 88  KDLFHPEQMVTGKEDASNNYARGHYTVGKE 117



 Score = 75.4 bits (177), Expect = 4e-15
 Identities = 32/61 (52%), Positives = 45/61 (73%)
 Frame = +2

Query: 251 KEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFVI 430
           KE++D VL+RIR++AD C+GLQGFL+FH            LL+ERL+++YGKKS L+F +
Sbjct: 116 KEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSV 175

Query: 431 Y 433
           Y
Sbjct: 176 Y 176


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 73.7 bits (173), Expect = 1e-14
 Identities = 31/65 (47%), Positives = 43/65 (66%)
 Frame = +3

Query: 57  DSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPQQLITGKEDAANNYARG 236
           +  N +F+E   GK+VPRAV VDLEP  +D V++G +  LF P  +I G+  A N +A+G
Sbjct: 45  ERLNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKG 104

Query: 237 HYTIG 251
           HYT G
Sbjct: 105 HYTEG 109



 Score = 37.1 bits (82), Expect = 0.001
 Identities = 19/69 (27%), Positives = 32/69 (46%), Gaps = 3/69 (4%)
 Frame = +2

Query: 233 WSLHHWKE---IVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYG 403
           W+  H+ E   + D VLD +R+ A+ C  LQGF + H            LL+ ++  +Y 
Sbjct: 101 WAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYP 160

Query: 404 KKSKLEFVI 430
            +    F +
Sbjct: 161 DRMMATFSV 169


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 59.3 bits (137), Expect = 2e-10
 Identities = 29/81 (35%), Positives = 49/81 (60%), Gaps = 2/81 (2%)
 Frame = +3

Query: 3   GIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFH 182
           GI PDG + +  T   G D  + FF ++   +++PRA+ +DLEP VV+ + + TY  L++
Sbjct: 30  GIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYGSLYN 87

Query: 183 PQQLITGKE--DAANNYARGH 239
           P+ ++  K    A NN+A G+
Sbjct: 88  PENILITKNGGGAGNNWANGY 108



 Score = 31.9 bits (69), Expect = 0.043
 Identities = 17/55 (30%), Positives = 26/55 (47%)
 Frame = +2

Query: 245 HWKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKK 409
           H + I + ++D I + AD    L+GF + H             L+ERL+  Y KK
Sbjct: 110 HAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKK 164


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 2.8
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +2

Query: 17  WPDAHRQDHRGWRRFFQHFLQR 82
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


>SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 649

 Score = 25.0 bits (52), Expect = 5.0
 Identities = 10/19 (52%), Positives = 13/19 (68%)
 Frame = +3

Query: 204 KEDAANNYARGHYTIGKKS 260
           +E  ANN+ RGH  I KK+
Sbjct: 599 RERKANNFFRGHIEIDKKT 617


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 24.2 bits (50), Expect = 8.7
 Identities = 14/34 (41%), Positives = 18/34 (52%)
 Frame = +1

Query: 1   TASSLMARCPQTRPSGVETILSTLSSARPELAST 102
           T SSL +    ++PS   T  ST SSA P   S+
Sbjct: 174 TFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,812,193
Number of Sequences: 5004
Number of extensions: 33809
Number of successful extensions: 101
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 92
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 101
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 154067960
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)