BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10874 (653 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g10360.1 68418.m01202 40S ribosomal protein S6 (RPS6B) 135 2e-32 At4g31700.1 68417.m04500 40S ribosomal protein S6 (RPS6A) riboso... 131 3e-31 At3g24080.1 68416.m03024 KRR1 family protein contains Pfam PF051... 31 0.67 At1g75300.1 68414.m08747 isoflavone reductase, putative identica... 30 1.2 At1g21740.1 68414.m02721 expressed protein contains Pfam domains... 30 1.5 At1g75290.1 68414.m08746 isoflavone reductase, putative similar ... 29 2.7 At1g67280.1 68414.m07657 lactoylglutathione lyase, putative / gl... 29 3.6 At1g21390.1 68414.m02676 expressed protein 28 4.7 At2g16390.1 68415.m01876 SNF2 domain-containing protein / helica... 28 6.2 At3g06860.1 68416.m00814 fatty acid multifunctional protein (MFP... 27 8.2 >At5g10360.1 68418.m01202 40S ribosomal protein S6 (RPS6B) Length = 249 Score = 135 bits (327), Expect = 2e-32 Identities = 73/116 (62%), Positives = 85/116 (73%), Gaps = 4/116 (3%) Frame = +2 Query: 257 KGHSCYRP--RRDGERKRKSVRGCIVDANLSVLALVIVRKGAQEIPGLTDGNVPRRLGPK 430 +G C+R RR GER+RKSVRGCIV +LSVL LVIV+KG ++PGLTD PR GPK Sbjct: 79 RGTPCFRGHGRRTGERRRKSVRGCIVSPDLSVLNLVIVKKGVSDLPGLTDTEKPRMRGPK 138 Query: 431 RASKIRKLFNLSKEDDVRRYV--VKRVLQLRKEKKTLNPDNKAPKIQRLVTPVVLQ 592 RASKIRKLFNL KEDDVR+YV +R +K KK +KAPKIQRLVTP+ LQ Sbjct: 139 RASKIRKLFNLGKEDDVRKYVNTYRRTFTNKKGKKV----SKAPKIQRLVTPLTLQ 190 Score = 112 bits (270), Expect = 2e-25 Identities = 50/81 (61%), Positives = 61/81 (75%) Frame = +3 Query: 24 MKLNVSYPATGCQKLFEVVDEHKLRIFYEKRMGAEVEADQLGDEWKGYVLRVAGGNDKQG 203 MK NV+ P TGCQK E+ D+ KLR F++KR+ EV D LG+E+KGYV ++ GG DKQG Sbjct: 1 MKFNVANPTTGCQKKLEIDDDQKLRAFFDKRLSQEVSGDALGEEFKGYVFKIMGGCDKQG 60 Query: 204 FPMKQGVLTNSRVRLLMQRAT 266 FPMKQGVLT RVRLL+ R T Sbjct: 61 FPMKQGVLTPGRVRLLLHRGT 81 >At4g31700.1 68417.m04500 40S ribosomal protein S6 (RPS6A) ribosomal protein S6, Arabidopsis thaliana, PID:g2662469 Length = 250 Score = 131 bits (317), Expect = 3e-31 Identities = 72/116 (62%), Positives = 84/116 (72%), Gaps = 4/116 (3%) Frame = +2 Query: 257 KGHSCYRP--RRDGERKRKSVRGCIVDANLSVLALVIVRKGAQEIPGLTDGNVPRRLGPK 430 +G C+R RR GER+RKSVRGCIV +LSVL LVIV+KG ++PGLTD PR GPK Sbjct: 79 RGTPCFRGHGRRTGERRRKSVRGCIVSPDLSVLNLVIVKKGENDLPGLTDTEKPRMRGPK 138 Query: 431 RASKIRKLFNLSKEDDVRRYV--VKRVLQLRKEKKTLNPDNKAPKIQRLVTPVVLQ 592 RASKIRKLFNL KEDDVR YV +R +K K+ +KAPKIQRLVTP+ LQ Sbjct: 139 RASKIRKLFNLKKEDDVRTYVNTYRRKFTNKKGKEV----SKAPKIQRLVTPLTLQ 190 Score = 113 bits (273), Expect = 7e-26 Identities = 51/81 (62%), Positives = 61/81 (75%) Frame = +3 Query: 24 MKLNVSYPATGCQKLFEVVDEHKLRIFYEKRMGAEVEADQLGDEWKGYVLRVAGGNDKQG 203 MK NV+ P TGCQK E+ D+ KLR FY+KR+ EV D LG+E+KGYV ++ GG DKQG Sbjct: 1 MKFNVANPTTGCQKKLEIDDDQKLRAFYDKRISQEVSGDALGEEFKGYVFKIKGGCDKQG 60 Query: 204 FPMKQGVLTNSRVRLLMQRAT 266 FPMKQGVLT RVRLL+ R T Sbjct: 61 FPMKQGVLTPGRVRLLLHRGT 81 >At3g24080.1 68416.m03024 KRR1 family protein contains Pfam PF05178: Krr1 family Length = 638 Score = 31.1 bits (67), Expect = 0.67 Identities = 13/45 (28%), Positives = 29/45 (64%) Frame = +2 Query: 392 LTDGNVPRRLGPKRASKIRKLFNLSKEDDVRRYVVKRVLQLRKEK 526 + +G+V ++ ++A + K + KEDDVR+ +KR+ ++K++ Sbjct: 288 VVEGSVRKKDNARKAQRKNKDERMKKEDDVRKEELKRLKNVKKKE 332 >At1g75300.1 68414.m08747 isoflavone reductase, putative identical to SP|P52577 Isoflavone reductase homolog P3 (EC 1.3.1.-) {Arabidopsis thaliana}; contains Pfam profile PF02716: Isoflavone reductase Length = 322 Score = 30.3 bits (65), Expect = 1.2 Identities = 16/41 (39%), Positives = 24/41 (58%), Gaps = 2/41 (4%) Frame = +2 Query: 461 LSKEDDVRRYVVKRVLQLRKEKKTL--NPDNKAPKIQRLVT 577 ++KE+DV Y++K V LR KTL +P N + +VT Sbjct: 205 INKEEDVAAYMIKAVDDLRTLNKTLYISPPNNILSMNEMVT 245 >At1g21740.1 68414.m02721 expressed protein contains Pfam domains, PF04782: Protein of unknown function (DUF632) and PF04783: Protein of unknown function (DUF630) Length = 953 Score = 29.9 bits (64), Expect = 1.5 Identities = 18/50 (36%), Positives = 26/50 (52%), Gaps = 1/50 (2%) Frame = +3 Query: 9 GLTAVMKLNVSYPATGCQKLF-EVVDEHKLRIFYEKRMGAEVEADQLGDE 155 GLT + + +KL+ EV DE KLR+ YE++ + D LG E Sbjct: 585 GLTGNLSATLEQLYAWEKKLYKEVKDEEKLRVVYEEKCRTLKKLDSLGAE 634 >At1g75290.1 68414.m08746 isoflavone reductase, putative similar to SP|P52577 Isoflavone reductase homolog P3 (EC 1.3.1.-) {Arabidopsis thaliana}; contains Pfam profile PF02716: Isoflavone reductase Length = 323 Score = 29.1 bits (62), Expect = 2.7 Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 2/41 (4%) Frame = +2 Query: 461 LSKEDDVRRYVVKRVLQLRKEKKTL--NPDNKAPKIQRLVT 577 ++KE+D+ Y +K V R KTL NP N + +VT Sbjct: 196 INKEEDIAAYTIKAVDDPRTLNKTLYINPPNNTLSMNEIVT 236 >At1g67280.1 68414.m07657 lactoylglutathione lyase, putative / glyoxalase I, putative similar to putative lactoylglutathione lyase SP:Q39366, GI:2494843 from [Brassica oleracea] Length = 350 Score = 28.7 bits (61), Expect = 3.6 Identities = 17/56 (30%), Positives = 26/56 (46%) Frame = +3 Query: 45 PATGCQKLFEVVDEHKLRIFYEKRMGAEVEADQLGDEWKGYVLRVAGGNDKQGFPM 212 P CQ + V D + FYEK G E+ + E+K Y + + G + FP+ Sbjct: 216 PEPLCQVMLRVGDLDRAIKFYEKAFGMELLRTRDNPEYK-YTIAMMGYGPEDKFPV 270 >At1g21390.1 68414.m02676 expressed protein Length = 248 Score = 28.3 bits (60), Expect = 4.7 Identities = 29/110 (26%), Positives = 45/110 (40%), Gaps = 1/110 (0%) Frame = +2 Query: 161 GLCTSCRWRQRQARIPDETGRPDKQPCSSSDAKGHSCYRPRRD-GERKRKSVRGCIVDAN 337 G+C+ WR + P RP+ S D+K + R +R+ E K C Sbjct: 28 GVCSPTLWRTSPPKSPPPFHRPEDYWSLSPDSKAQAIARGQRELMEMVSKMPESCY---E 84 Query: 338 LSVLALVIVRKGAQEIPGLTDGNVPRRLGPKRASKIRKLFNLSKEDDVRR 487 LS+ LV V+ + + D +P+R R SK+ + K D R Sbjct: 85 LSLKDLVEVKVNQENERKVFD-ELPKR--ANRQSKVVRKTKSDKRVDPNR 131 >At2g16390.1 68415.m01876 SNF2 domain-containing protein / helicase domain-containing protein low similarity to RAD54 [Drosophila melanogaster] GI:1765914; contains Pfam profiles PF00271: Helicase conserved C-terminal domain, PF00176: SNF2 family N-terminal domain Length = 888 Score = 27.9 bits (59), Expect = 6.2 Identities = 11/25 (44%), Positives = 17/25 (68%) Frame = +2 Query: 380 EIPGLTDGNVPRRLGPKRASKIRKL 454 E+PGL D V L PK+ ++++KL Sbjct: 620 ELPGLADFTVVLNLSPKQLNEVKKL 644 >At3g06860.1 68416.m00814 fatty acid multifunctional protein (MFP2) identical to fatty acid multifunctional protein (AtMFP2) GB:AF123254 [gi:4337027] (Arabidopsis thaliana) (fatty acid beta-oxidation); contains Pfam profiles PF02737 (3-hydroxyacyl-CoA dehydrogenase, NAD binding domain), PF00378 (enoyl-CoA hydratase/isomerase family protein), PF00725 (3-hydroxyacyl-CoA dehydrogenase) Length = 725 Score = 27.5 bits (58), Expect = 8.2 Identities = 19/67 (28%), Positives = 30/67 (44%), Gaps = 3/67 (4%) Frame = +2 Query: 230 KQPCSSSDAKGHSCYRPRRDGERKRKSVRGCIV--DANLSVLALVIVRKGAQEIPGLTD- 400 K P DA R G K V +V D ++ + ++G ++PG+TD Sbjct: 246 KHPLMCLDAIEVGIVSGPRAGLEKEAEVASQVVKLDTTKGLIHVFFSQRGTAKVPGVTDR 305 Query: 401 GNVPRRL 421 G VPR++ Sbjct: 306 GLVPRKI 312 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,746,483 Number of Sequences: 28952 Number of extensions: 293756 Number of successful extensions: 830 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 807 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 828 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1363910256 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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