Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV10869
(391 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g16030.1 68414.m01924 heat shock protein 70, putative / HSP70... 77 4e-15
At5g02500.1 68418.m00183 heat shock cognate 70 kDa protein 1 (HS... 73 9e-14
At5g02490.1 68418.m00182 heat shock cognate 70 kDa protein 2 (HS... 72 2e-13
At3g09440.1 68416.m01121 heat shock cognate 70 kDa protein 3 (HS... 71 2e-13
At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70... 71 4e-13
At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein, puta... 68 3e-12
At5g42020.2 68418.m05116 luminal binding protein 2 (BiP-2) (BP2)... 62 2e-10
At5g42020.1 68418.m05115 luminal binding protein 2 (BiP-2) (BP2)... 62 2e-10
At5g28540.1 68418.m03480 luminal binding protein 1 (BiP-1) (BP1)... 62 2e-10
At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)... 56 6e-09
At1g14650.1 68414.m01741 SWAP (Suppressor-of-White-APricot)/surp... 31 0.27
At5g01570.1 68418.m00072 hypothetical protein hypothetical prote... 30 0.63
At1g14640.1 68414.m01740 SWAP (Suppressor-of-White-APricot)/surp... 29 1.5
At1g79930.1 68414.m09340 heat shock protein, putative contains P... 28 2.6
At5g60030.1 68418.m07527 expressed protein 27 3.4
At2g01270.1 68415.m00040 thioredoxin family protein low similari... 27 3.4
At3g19050.1 68416.m02420 kinesin motor protein-related contains ... 27 4.5
At5g24880.1 68418.m02946 expressed protein ; expression supporte... 27 5.9
At3g50180.1 68416.m05486 hypothetical protein 27 5.9
At3g25680.1 68416.m03196 expressed protein 26 7.8
At1g11730.1 68414.m01346 galactosyltransferase family protein co... 26 7.8
>At1g16030.1 68414.m01924 heat shock protein 70, putative / HSP70,
putative similar to heat shock protein hsp70 GI:1771478
from [Pisum sativum]
Length = 646
Score = 77.0 bits (181), Expect = 4e-15
Identities = 33/59 (55%), Positives = 47/59 (79%)
Frame = +2
Query: 2 EDEKLKEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 178
+DEKL +K++ DKQ I ++TI+W++ NQLA+ +E+E+K KELEGI NPII+KMYQ
Sbjct: 559 KDEKLAQKLTQEDKQKIEKAIDETIEWIEGNQLAEVDEFEYKLKELEGICNPIISKMYQ 617
>At5g02500.1 68418.m00183 heat shock cognate 70 kDa protein 1
(HSC70-1) (HSP70-1) identical to SP|P22953 Heat shock
cognate 70 kDa protein 1 (Hsc70.1) {Arabidopsis
thaliana}
Length = 651
Score = 72.5 bits (170), Expect = 9e-14
Identities = 32/59 (54%), Positives = 42/59 (71%)
Frame = +2
Query: 2 EDEKLKEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 178
+DEK+ EK+ +DK+ I D I+WL+ NQLA+ +E+E K KELE I NPII KMYQ
Sbjct: 560 QDEKIGEKLPAADKKKIEDSIEQAIQWLEGNQLAEADEFEDKMKELESICNPIIAKMYQ 618
>At5g02490.1 68418.m00182 heat shock cognate 70 kDa protein 2
(HSC70-2) (HSP70-2) identical to SP|P22954 Heat shock
cognate 70 kDa protein 2 (Hsc70.2) {Arabidopsis
thaliana}
Length = 653
Score = 71.7 bits (168), Expect = 2e-13
Identities = 30/58 (51%), Positives = 41/58 (70%)
Frame = +2
Query: 5 DEKLKEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 178
DEK+ EK+ +DK+ + D + I+WLD NQL + +E+E K KELE + NPII KMYQ
Sbjct: 561 DEKIGEKLPAADKKKVEDSIEEAIQWLDGNQLGEADEFEDKMKELESVCNPIIAKMYQ 618
>At3g09440.1 68416.m01121 heat shock cognate 70 kDa protein 3
(HSC70-3) (HSP70-3) identical to SP|O65719 Heat shock
cognate 70 kDa protein 3 (Hsc70.3) {Arabidopsis
thaliana}
Length = 649
Score = 71.3 bits (167), Expect = 2e-13
Identities = 32/58 (55%), Positives = 42/58 (72%)
Frame = +2
Query: 5 DEKLKEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 178
DEK+ EK++ DK+ I D I+WL++NQLA+ +E+E K KELE I NPII KMYQ
Sbjct: 561 DEKIGEKLAGDDKKKIEDSIEAAIEWLEANQLAECDEFEDKMKELESICNPIIAKMYQ 618
>At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70,
putative strong similarity to heat shock protein
GI:425194 [Spinacia oleracea]
Length = 650
Score = 70.5 bits (165), Expect = 4e-13
Identities = 30/59 (50%), Positives = 42/59 (71%)
Frame = +2
Query: 2 EDEKLKEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 178
+DEK+ K+ +DK+ I D + I+WLD NQLA+ +E+E K KELE + NPII +MYQ
Sbjct: 560 KDEKIASKLDAADKKKIEDAIDQAIEWLDGNQLAEADEFEDKMKELESLCNPIIARMYQ 618
>At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein,
putative / HSC70, putative / HSP70, putative strong
similarity to heat shock cognate 70 kd protein 1
SP:P22953 [Arabidopsis thaliana (Mouse-ear cress)]
Length = 617
Score = 67.7 bits (158), Expect = 3e-12
Identities = 28/53 (52%), Positives = 39/53 (73%)
Frame = +2
Query: 20 EKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 178
EK+ +DK+ D + I+WLD NQLA+ +E+EHK KELE +++ IITKMYQ
Sbjct: 564 EKLPAADKKKFEDSIEEVIQWLDDNQLAEADEFEHKMKELESVWSTIITKMYQ 616
>At5g42020.2 68418.m05116 luminal binding protein 2 (BiP-2) (BP2)
similar to SWISS-PROT: Q39043; GI:1303695; luminal
binding protein (BiP) [Arabidopsis thaliana]
Length = 613
Score = 61.7 bits (143), Expect = 2e-10
Identities = 25/59 (42%), Positives = 40/59 (67%)
Frame = +2
Query: 2 EDEKLKEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 178
+ +KL +K+ +K+ I + ++WLD NQ ++KEEY+ K KE+E + NPIIT +YQ
Sbjct: 531 DKDKLADKLEGDEKEKIEAATKEALEWLDENQNSEKEEYDEKLKEVEAVCNPIITAVYQ 589
>At5g42020.1 68418.m05115 luminal binding protein 2 (BiP-2) (BP2)
similar to SWISS-PROT: Q39043; GI:1303695; luminal
binding protein (BiP) [Arabidopsis thaliana]
Length = 668
Score = 61.7 bits (143), Expect = 2e-10
Identities = 25/59 (42%), Positives = 40/59 (67%)
Frame = +2
Query: 2 EDEKLKEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 178
+ +KL +K+ +K+ I + ++WLD NQ ++KEEY+ K KE+E + NPIIT +YQ
Sbjct: 586 DKDKLADKLEGDEKEKIEAATKEALEWLDENQNSEKEEYDEKLKEVEAVCNPIITAVYQ 644
>At5g28540.1 68418.m03480 luminal binding protein 1 (BiP-1) (BP1)
SWISS-PROT:Q9LKR3 PMID:8888624
Length = 669
Score = 61.7 bits (143), Expect = 2e-10
Identities = 25/59 (42%), Positives = 40/59 (67%)
Frame = +2
Query: 2 EDEKLKEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 178
+ +KL +K+ +K+ I + ++WLD NQ ++KEEY+ K KE+E + NPIIT +YQ
Sbjct: 586 DKDKLADKLEGDEKEKIEAATKEALEWLDENQNSEKEEYDEKLKEVEAVCNPIITAVYQ 644
>At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)
Similar to Arabidopsis luminal binding protein
(gb|D89342); contains Pfam domain PF00012: dnaK protein
Length = 678
Score = 56.4 bits (130), Expect = 6e-09
Identities = 23/59 (38%), Positives = 40/59 (67%)
Frame = +2
Query: 2 EDEKLKEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 178
+ EKL +KISD DK+ + + ++WL+ N A+KE+Y+ K KE+E + +P+I +Y+
Sbjct: 600 DKEKLAKKISDEDKEKMEGVLKEALEWLEENVNAEKEDYDEKLKEVELVCDPVIKSVYE 658
>At1g14650.1 68414.m01741 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein / ubiquitin family protein
similar to SP|Q15459 Splicing factor 3 subunit 1
(Spliceosome associated protein 114) {Homo sapiens};
contains Pfam profiles PF00240: Ubiquitin family,
PF01805: Surp module
Length = 785
Score = 31.1 bits (67), Expect = 0.27
Identities = 19/52 (36%), Positives = 32/52 (61%), Gaps = 2/52 (3%)
Frame = +2
Query: 14 LKEKI--SDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPII 163
LKEK+ S +D T+L++C ++W D +Q K++ E +KELE + +I
Sbjct: 252 LKEKLRKSAADLTTVLERCLHRLEW-DRSQEQQKKK-EEDEKELERVQMAMI 301
>At5g01570.1 68418.m00072 hypothetical protein hypothetical protein
T16O11.19 - Arabidopsis thaliana, EMBL:AC010871
Length = 157
Score = 29.9 bits (64), Expect = 0.63
Identities = 21/48 (43%), Positives = 28/48 (58%), Gaps = 2/48 (4%)
Frame = +2
Query: 5 DEKLKEKISDS--DKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELE 142
DE KE I +S D Q LD C + +S ++AD+EE E QKEL+
Sbjct: 46 DEDFKE-IHESLQDLQKKLDVCKEKTDEANS-EIADEEEIERLQKELD 91
>At1g14640.1 68414.m01740 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein similar to human splicing
factor GB:CAA59494 GI:899298 from [Homo sapiens];
contains Pfam profile PF01805: Surp module
Length = 735
Score = 28.7 bits (61), Expect = 1.5
Identities = 14/43 (32%), Positives = 25/43 (58%), Gaps = 2/43 (4%)
Frame = +2
Query: 14 LKEKISDS--DKQTILDKCNDTIKWLDSNQLADKEEYEHKQKE 136
LKEK+ S D T+L++C + ++W + +E + K+KE
Sbjct: 243 LKEKLRKSVADLTTVLERCLNRLEWDRFQEEEKNKEEDEKEKE 285
>At1g79930.1 68414.m09340 heat shock protein, putative contains Pfam
profile: PF00012 Heat shock hsp70 proteins; similar to
heat-shock proteins GB:CAA94389, GB:AAD55461
[Arabidopsis thaliana]
Length = 831
Score = 27.9 bits (59), Expect = 2.6
Identities = 14/52 (26%), Positives = 28/52 (53%), Gaps = 1/52 (1%)
Frame = +2
Query: 8 EKLKEKISDSDKQTILDKCNDTIKWL-DSNQLADKEEYEHKQKELEGIYNPI 160
+K +E I+DS+++ L + WL + + K Y K +EL+ + +P+
Sbjct: 639 DKYQEYITDSEREAFLANLQEVEDWLYEDGEDETKGVYVAKLEELKKVGDPV 690
>At5g60030.1 68418.m07527 expressed protein
Length = 292
Score = 27.5 bits (58), Expect = 3.4
Identities = 17/48 (35%), Positives = 25/48 (52%), Gaps = 2/48 (4%)
Frame = +2
Query: 5 DEKLKEKISDSDKQTILD--KCNDTIKWLDSNQLADKEEYEHKQKELE 142
DEK+KEK+ D K K + K D + + +KE+ E +QK E
Sbjct: 166 DEKVKEKLEDEQKSADRKERKKKKSKKNNDEDVVDEKEKLEDEQKSAE 213
>At2g01270.1 68415.m00040 thioredoxin family protein low similarity
to quiescin [Homo sapiens] GI:13257405; contains Pfam
profiles PF00085: Thioredoxin, PF04777: Erv1 / Alr
family
Length = 495
Score = 27.5 bits (58), Expect = 3.4
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = +2
Query: 80 WLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 178
+L SNQ + + ++EH K L+ Y P + +Y+
Sbjct: 402 YLSSNQKSIEWDHEHVYKFLKNYYGPKLVSLYK 434
>At3g19050.1 68416.m02420 kinesin motor protein-related contains Pfam
profile: PF00225 Kinesin motor domain; contains
non-consensus splice site (GC) at intron 12
Length = 2722
Score = 27.1 bits (57), Expect = 4.5
Identities = 13/38 (34%), Positives = 23/38 (60%)
Frame = +2
Query: 35 SDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGI 148
+D++T + + ++ I L+ + A EY HK KELE +
Sbjct: 2336 ADQKTEITQLSEHISELNLHAEAQASEYMHKFKELEAM 2373
>At5g24880.1 68418.m02946 expressed protein ; expression supported
by MPSS
Length = 443
Score = 26.6 bits (56), Expect = 5.9
Identities = 14/48 (29%), Positives = 30/48 (62%), Gaps = 2/48 (4%)
Frame = +2
Query: 2 EDEKLKEKISDSD-KQTILDKCNDTIKW-LDSNQLADKEEYEHKQKEL 139
E+EK KEK+ + D K+ + ++ + +K + ++ ++E E K+KE+
Sbjct: 349 EEEKEKEKVKEDDQKEKVEEEEKEKVKGDEEKEKVKEEESAEGKKKEV 396
>At3g50180.1 68416.m05486 hypothetical protein
Length = 588
Score = 26.6 bits (56), Expect = 5.9
Identities = 14/25 (56%), Positives = 15/25 (60%), Gaps = 1/25 (4%)
Frame = -3
Query: 266 PAPPPR-GRHLRLRVLRPGSPAYLR 195
P PPPR G LRLR+L P LR
Sbjct: 38 PPPPPRLGPRLRLRLLPPPRQQLLR 62
>At3g25680.1 68416.m03196 expressed protein
Length = 558
Score = 26.2 bits (55), Expect = 7.8
Identities = 12/55 (21%), Positives = 30/55 (54%)
Frame = +2
Query: 8 EKLKEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKM 172
E+LKEK + ++ +L+ + I + ++DK Y + +L+ + + + +K+
Sbjct: 453 ERLKEKAAIDCQKQLLNSLTEEIDEMSQRLISDKSVYLTEHSKLQEMLSDLQSKL 507
>At1g11730.1 68414.m01346 galactosyltransferase family protein
contains Pfam profile: PF01762 galactosyltransferase
Length = 384
Score = 26.2 bits (55), Expect = 7.8
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +2
Query: 11 KLKEKISDSDKQTILDKCNDTIKWLDSN 94
KL SD DK+ +LD N+TI LD +
Sbjct: 55 KLSLSSSDCDKKNVLDYGNNTIGILDKS 82
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,469,601
Number of Sequences: 28952
Number of extensions: 145950
Number of successful extensions: 614
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 592
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 614
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 557595584
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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