BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10808 (620 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 146 2e-36 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 145 4e-36 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 122 4e-29 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 107 1e-24 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 67 2e-12 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 60 3e-10 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 57 2e-09 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 57 2e-09 SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 ... 26 3.8 SPCC584.05 |sec1||SNARE binding protein Sec1|Schizosaccharomyces... 26 3.8 SPCC4G3.18 |||conserved fungal family|Schizosaccharomyces pombe|... 26 5.1 SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|ch... 26 5.1 SPCC777.03c |||nifs homolog|Schizosaccharomyces pombe|chr 3|||Ma... 26 5.1 SPAC9.10 |||amino acid permease, unknown 2|Schizosaccharomyces p... 25 6.7 SPBC30D10.14 |||dienelactone hydrolase family|Schizosaccharomyce... 25 8.8 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 146 bits (355), Expect = 2e-36 Identities = 70/85 (82%), Positives = 79/85 (92%) Frame = +3 Query: 3 TLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIH 182 TLSSS QASIEIDSLFEGIDFYTSITRARFEEL ADLFR TMEPVE+ LRD+K+DKA ++ Sbjct: 271 TLSSSAQASIEIDSLFEGIDFYTSITRARFEELCADLFRKTMEPVERVLRDSKVDKASVN 330 Query: 183 DIVLVGGSTRIPKVQKLLQDFFNGR 257 +IVLVGGSTRIP+VQKL+ DFFNG+ Sbjct: 331 EIVLVGGSTRIPRVQKLVSDFFNGK 355 Score = 74.5 bits (175), Expect = 1e-14 Identities = 44/98 (44%), Positives = 49/98 (50%) Frame = +2 Query: 215 PQGAEAPARFL*WKELNKSINPDEXXXXXXXXXXXILHGDKSEEVQDXXXXXXXXXXXGI 394 P+ + + F KE KSINPDE +L GD SE+ QD GI Sbjct: 342 PRVQKLVSDFFNGKEPCKSINPDEAVAYGAAVQAAVLTGDTSEKTQDLLLLDVAPLSMGI 401 Query: 395 ETAGGVMTTLIKRNXXXXXXXXXXXXXYSDNQPGVLIQ 508 ETAGGVMT LIKRN YSDNQPGVLIQ Sbjct: 402 ETAGGVMTPLIKRNTTIPTKKSEIFSTYSDNQPGVLIQ 439 Score = 64.1 bits (149), Expect = 2e-11 Identities = 28/32 (87%), Positives = 30/32 (93%) Frame = +1 Query: 508 IFEGERAMTKDNNLLGKFELTGIPPAPRGVPQ 603 +FEGERA TKD NLLGKFEL+GIPPAPRGVPQ Sbjct: 440 VFEGERARTKDCNLLGKFELSGIPPAPRGVPQ 471 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 145 bits (352), Expect = 4e-36 Identities = 68/85 (80%), Positives = 80/85 (94%) Frame = +3 Query: 3 TLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIH 182 TLSSS QASIEIDSL+EGIDFYTSITRARFEEL ADLFR+TMEPVEK LRD+K+DK+ ++ Sbjct: 271 TLSSSAQASIEIDSLYEGIDFYTSITRARFEELCADLFRNTMEPVEKVLRDSKIDKSSVN 330 Query: 183 DIVLVGGSTRIPKVQKLLQDFFNGR 257 +IVLVGGSTRIP++QKL+ DFFNG+ Sbjct: 331 EIVLVGGSTRIPRIQKLVSDFFNGK 355 Score = 72.9 bits (171), Expect = 3e-14 Identities = 42/85 (49%), Positives = 44/85 (51%) Frame = +2 Query: 254 KELNKSINPDEXXXXXXXXXXXILHGDKSEEVQDXXXXXXXXXXXGIETAGGVMTTLIKR 433 KE KSINPDE IL GD SE+ QD GIETAGGVMT LIKR Sbjct: 355 KEPCKSINPDEAVAYGAAVQAAILVGDTSEKTQDLLLLDVAPLSLGIETAGGVMTPLIKR 414 Query: 434 NXXXXXXXXXXXXXYSDNQPGVLIQ 508 N Y+DNQPGVLIQ Sbjct: 415 NTTIPTKKSEVFSTYADNQPGVLIQ 439 Score = 64.1 bits (149), Expect = 2e-11 Identities = 28/32 (87%), Positives = 30/32 (93%) Frame = +1 Query: 508 IFEGERAMTKDNNLLGKFELTGIPPAPRGVPQ 603 +FEGERA TKD NLLGKFEL+GIPPAPRGVPQ Sbjct: 440 VFEGERARTKDCNLLGKFELSGIPPAPRGVPQ 471 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 122 bits (294), Expect = 4e-29 Identities = 55/85 (64%), Positives = 73/85 (85%) Frame = +3 Query: 6 LSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHD 185 LSS TQ ++E+DSL GIDF +SITRARFE++NA F++T++PV K L+D+K+ KA +HD Sbjct: 276 LSSVTQTTVEVDSLSNGIDFSSSITRARFEDINATTFKATIDPVAKVLKDSKVPKADVHD 335 Query: 186 IVLVGGSTRIPKVQKLLQDFFNGRS 260 IVLVGGSTRIPKVQ+L+ DFF+GR+ Sbjct: 336 IVLVGGSTRIPKVQRLVSDFFDGRA 360 Score = 47.2 bits (107), Expect = 2e-06 Identities = 19/35 (54%), Positives = 25/35 (71%) Frame = +1 Query: 490 TRSTHPIFEGERAMTKDNNLLGKFELTGIPPAPRG 594 T T P+++GER +N LG+F+LTGIPP PRG Sbjct: 439 TTVTFPVYQGERVNCAENEPLGEFQLTGIPPMPRG 473 Score = 30.3 bits (65), Expect = 0.23 Identities = 22/83 (26%), Positives = 30/83 (36%), Gaps = 1/83 (1%) Frame = +2 Query: 254 KELNKSINPDEXXXXXXXXXXXIL-HGDKSEEVQDXXXXXXXXXXXGIETAGGVMTTLIK 430 + LNKSINPDE +L + S++ QD G+ G V + Sbjct: 359 RALNKSINPDEAVAYGAAVQAAVLTNKADSDKTQDLLLLDVVPLSLGVAMEGNVFGVVCP 418 Query: 431 RNXXXXXXXXXXXXXYSDNQPGV 499 RN +DNQ V Sbjct: 419 RNTPIPTIKKRTFTTVADNQTTV 441 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 107 bits (258), Expect = 1e-24 Identities = 49/90 (54%), Positives = 69/90 (76%) Frame = +3 Query: 3 TLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIH 182 TLSS IEI+S F G DF +++RA+FEE+N DLF+ T++PVE+ L+D+ + K++I Sbjct: 303 TLSSQKSVRIEIESFFNGQDFSETLSRAKFEEINMDLFKKTLKPVEQVLKDSNLKKSEID 362 Query: 183 DIVLVGGSTRIPKVQKLLQDFFNGRSSTNL 272 DIVLVGGSTRIPKVQ+LL+ FF ++S + Sbjct: 363 DIVLVGGSTRIPKVQELLESFFGKKASKGI 392 Score = 64.1 bits (149), Expect = 2e-11 Identities = 26/32 (81%), Positives = 30/32 (93%) Frame = +1 Query: 508 IFEGERAMTKDNNLLGKFELTGIPPAPRGVPQ 603 ++EGER +TKDNNLLGKF+L GIPPAPRGVPQ Sbjct: 469 VYEGERTLTKDNNLLGKFDLRGIPPAPRGVPQ 500 Score = 43.2 bits (97), Expect = 3e-05 Identities = 29/85 (34%), Positives = 36/85 (42%) Frame = +2 Query: 254 KELNKSINPDEXXXXXXXXXXXILHGDKSEEVQDXXXXXXXXXXXGIETAGGVMTTLIKR 433 K+ +K INPDE +L G++ + + GIET GGVMT LI R Sbjct: 386 KKASKGINPDEAVAYGAAVQAGVLSGEEGSD--NIVLLDVIPLTLGIETTGGVMTKLIGR 443 Query: 434 NXXXXXXXXXXXXXYSDNQPGVLIQ 508 N DNQ VLIQ Sbjct: 444 NTPIPTRKSQIFSTAVDNQNTVLIQ 468 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 67.3 bits (157), Expect = 2e-12 Identities = 31/80 (38%), Positives = 53/80 (66%) Frame = +3 Query: 6 LSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHD 185 LS++++A I+ L +GIDF ITR+ E L D+ + +EP+ K+L+ A + ++I+ Sbjct: 308 LSANSEAIASIEELADGIDFRLKITRSVLESLCKDMEDAAVEPINKALKKANLTFSEINS 367 Query: 186 IVLVGGSTRIPKVQKLLQDF 245 I+L GG++RIP +Q L D+ Sbjct: 368 IILFGGASRIPFIQSTLADY 387 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 59.7 bits (138), Expect = 3e-10 Identities = 27/81 (33%), Positives = 48/81 (59%) Frame = +3 Query: 6 LSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHD 185 LS+ T AS +DSL +GIDF++SI R R++ + + V +++ A M+ I + Sbjct: 298 LSNGTTASAAVDSLADGIDFHSSINRLRYDLAASATLNRMADLVTEAVEKANMEPFDISE 357 Query: 186 IVLVGGSTRIPKVQKLLQDFF 248 ++L GG++ PK+ L++ F Sbjct: 358 VILAGGASNTPKLTSLMESIF 378 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 57.2 bits (132), Expect = 2e-09 Identities = 23/58 (39%), Positives = 44/58 (75%) Frame = +3 Query: 75 ITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFF 248 I+RA+FE+L L R T++P +++L+DA + ++I++++LVGG TR+P+V + ++ F Sbjct: 343 ISRAQFEKLVDPLVRRTIDPCKRALKDANLQTSEINEVILVGGMTRMPRVVETVKSIF 400 Score = 50.8 bits (116), Expect = 2e-07 Identities = 19/32 (59%), Positives = 26/32 (81%) Frame = +1 Query: 508 IFEGERAMTKDNNLLGKFELTGIPPAPRGVPQ 603 +F+GER + +DN L+G F+LTGI PAP+G PQ Sbjct: 483 VFQGERELVRDNKLIGNFQLTGIAPAPKGQPQ 514 Score = 37.1 bits (82), Expect = 0.002 Identities = 27/85 (31%), Positives = 33/85 (38%) Frame = +2 Query: 254 KELNKSINPDEXXXXXXXXXXXILHGDKSEEVQDXXXXXXXXXXXGIETAGGVMTTLIKR 433 +E KS+NPDE +L G V+D GIET GGV T LI R Sbjct: 402 REPAKSVNPDEAVAIGAAIQGGVLSG----HVKDLVLLDVTPLSLGIETLGGVFTRLINR 457 Query: 434 NXXXXXXXXXXXXXYSDNQPGVLIQ 508 N +D Q V I+ Sbjct: 458 NTTIPTRKSQVFSTAADGQTAVEIR 482 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 57.2 bits (132), Expect = 2e-09 Identities = 27/81 (33%), Positives = 48/81 (59%) Frame = +3 Query: 6 LSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHD 185 LS++ A + ++ + ID + I R+ FEEL L P+EK+L A + K ++ Sbjct: 280 LSANANAPLNVEMIMNDIDASSFIKRSDFEELIKPLLERLTPPIEKALELAGIKKEDLYS 339 Query: 186 IVLVGGSTRIPKVQKLLQDFF 248 I +VGG TR+P V++++ ++F Sbjct: 340 IEMVGGCTRVPIVKEVIANYF 360 >SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 932 Score = 26.2 bits (55), Expect = 3.8 Identities = 9/19 (47%), Positives = 14/19 (73%) Frame = -3 Query: 183 RGFEPYPSWHHGETSPLAP 127 + +P P+ HG+TSPL+P Sbjct: 420 KAIKPMPTSIHGQTSPLSP 438 >SPCC584.05 |sec1||SNARE binding protein Sec1|Schizosaccharomyces pombe|chr 3|||Manual Length = 693 Score = 26.2 bits (55), Expect = 3.8 Identities = 19/79 (24%), Positives = 36/79 (45%) Frame = -2 Query: 514 QILDEYSGLVIRVGGECLSLFSGDGSVTLDECGHDTSSSLNTEGKGCYIKQQQILHLLRL 335 ++ D+YS L + + EC+++F + D S+ N EGK ++L LL Sbjct: 340 ELRDQYS-LHLTMAQECMNIFEKQQLNLIGAIEQDLSTGSNVEGKVPRSVLSELLPLLDE 398 Query: 334 VTVQDSSLDSCTISYGLVR 278 ++S+ + Y + R Sbjct: 399 GNAEESTKIRLLLLYIIYR 417 >SPCC4G3.18 |||conserved fungal family|Schizosaccharomyces pombe|chr 3|||Manual Length = 828 Score = 25.8 bits (54), Expect = 5.1 Identities = 14/46 (30%), Positives = 22/46 (47%) Frame = -2 Query: 322 DSSLDSCTISYGLVRVNRFVELLPLKKSCRSFCTLGIRVEPPTSTI 185 DS + C S+ + + L L ++ CTL I + PTST+ Sbjct: 472 DSDVMDCCFSFLASILTHSIALPRLLRTKIDLCTLQISLSNPTSTV 517 >SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|chr 2|||Manual Length = 538 Score = 25.8 bits (54), Expect = 5.1 Identities = 17/53 (32%), Positives = 27/53 (50%) Frame = +3 Query: 42 SLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHDIVLVG 200 SL G+D+YT I E +A +S+ E +K D + D++ D + VG Sbjct: 331 SLARGLDYYTGIIYEAVTEASAPKIKSSAE--KKKSADPEADRSN-DDSIGVG 380 >SPCC777.03c |||nifs homolog|Schizosaccharomyces pombe|chr 3|||Manual Length = 396 Score = 25.8 bits (54), Expect = 5.1 Identities = 10/39 (25%), Positives = 21/39 (53%) Frame = -2 Query: 301 TISYGLVRVNRFVELLPLKKSCRSFCTLGIRVEPPTSTI 185 T+S G+ R+ F + +PL + + + +PP ++I Sbjct: 2 TVSEGIARIQAFRKQVPLVEKNENITFFNLSFQPPMNSI 40 >SPAC9.10 |||amino acid permease, unknown 2|Schizosaccharomyces pombe|chr 1|||Manual Length = 591 Score = 25.4 bits (53), Expect = 6.7 Identities = 13/34 (38%), Positives = 18/34 (52%), Gaps = 4/34 (11%) Frame = +1 Query: 109 ICSGLPWSQ----WRSLSVMPRWIRLKSTILYWW 198 ICS LP + W + S PR+ R S ++ WW Sbjct: 133 ICSALPAAGSIYLWAAESAGPRFGRFVSFLVAWW 166 >SPBC30D10.14 |||dienelactone hydrolase family|Schizosaccharomyces pombe|chr 2|||Manual Length = 249 Score = 25.0 bits (52), Expect = 8.8 Identities = 11/21 (52%), Positives = 14/21 (66%) Frame = +3 Query: 210 RIPKVQKLLQDFFNGRSSTNL 272 + P+ QKL DFF+ R S NL Sbjct: 91 KTPEDQKLCNDFFSTRISPNL 111 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,539,459 Number of Sequences: 5004 Number of extensions: 50513 Number of successful extensions: 172 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 153 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 172 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 273658928 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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