BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10776 (625 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 185 5e-48 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 98 1e-21 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 85 1e-17 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 58 8e-10 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 44 1e-05 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 42 5e-05 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 40 4e-04 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 31 0.14 SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3 |Schizos... 29 0.72 SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 28 0.95 SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces p... 27 2.2 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 27 2.2 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 2.9 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 3.8 SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|S... 26 5.1 SPAC1F7.05 |cdc22||ribonucleoside reductase large subunit Cdc22|... 26 5.1 SPCC1906.04 |wtf20||wtf element Wtf20|Schizosaccharomyces pombe|... 25 6.7 SPBC16A3.11 |eso1||sister chromatid cohesion protein Eso1|Schizo... 25 6.7 SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo... 25 8.9 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 185 bits (450), Expect = 5e-48 Identities = 81/84 (96%), Positives = 82/84 (97%) Frame = +2 Query: 257 GMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHG 436 GMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSKRGILTLKYPIEHG Sbjct: 15 GMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSKRGILTLKYPIEHG 74 Query: 437 IVTNWDDMEKIWHHTFYNELRVAP 508 IV NWDDMEKIWHHTFYNELRVAP Sbjct: 75 IVNNWDDMEKIWHHTFYNELRVAP 98 Score = 78.2 bits (184), Expect = 9e-16 Identities = 34/40 (85%), Positives = 37/40 (92%) Frame = +1 Query: 505 PQEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQ 624 P+EHP LLTEAPLNPK+NREKMTQI+FETFN PA YVAIQ Sbjct: 98 PEEHPCLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQ 137 Score = 25.0 bits (52), Expect = 8.9 Identities = 9/12 (75%), Positives = 12/12 (100%) Frame = +3 Query: 219 DEEVAALVVDNG 254 +EE+AALV+DNG Sbjct: 2 EEEIAALVIDNG 13 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 97.9 bits (233), Expect = 1e-21 Identities = 41/79 (51%), Positives = 57/79 (72%) Frame = +2 Query: 257 GMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHG 436 G KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG+L ++ PIE G Sbjct: 19 GFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKIQRPIERG 78 Query: 437 IVTNWDDMEKIWHHTFYNE 493 I+ NW DME+IW + + ++ Sbjct: 79 IIQNWSDMEEIWSYIYSDQ 97 Score = 47.2 bits (107), Expect = 2e-06 Identities = 19/40 (47%), Positives = 28/40 (70%) Frame = +1 Query: 505 PQEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQ 624 P+EHP+LLTE PL N+EK+ + +ET N PA+ ++Q Sbjct: 103 PEEHPLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQ 142 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 84.6 bits (200), Expect = 1e-17 Identities = 38/87 (43%), Positives = 60/87 (68%), Gaps = 2/87 (2%) Frame = +2 Query: 257 GMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIE 430 G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ R +L +KYP+E Sbjct: 13 GFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRSLLQVKYPME 72 Query: 431 HGIVTNWDDMEKIWHHTFYNELRVAPR 511 +GI+ ++++M ++W +TF+ +L++ PR Sbjct: 73 NGIIRDFEEMNQLWDYTFFEKLKIDPR 99 Score = 50.0 bits (114), Expect = 3e-07 Identities = 22/40 (55%), Positives = 28/40 (70%) Frame = +1 Query: 505 PQEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQ 624 P+ +LLTE P+NP ANREKM + MFE + +YVAIQ Sbjct: 98 PRGRKILLTEPPMNPVANREKMCETMFERYGFGGVYVAIQ 137 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 58.4 bits (135), Expect = 8e-10 Identities = 23/42 (54%), Positives = 30/42 (71%) Frame = +1 Query: 499 CRPQEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQ 624 C P++H LLTE PLNP NRE +IMFE+FN +Y+A+Q Sbjct: 119 CEPEDHYFLLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQ 160 Score = 52.8 bits (121), Expect = 4e-08 Identities = 35/110 (31%), Positives = 50/110 (45%), Gaps = 24/110 (21%) Frame = +2 Query: 257 GMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK--------------D 367 G K G+AG+DAP VFP+++ R G G M K D Sbjct: 15 GYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSKRATEDLD 73 Query: 368 SYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPRN 514 ++G++A K +L YPI HG + NWD ME+ W + + LR P + Sbjct: 74 FFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPED 123 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 44.4 bits (100), Expect = 1e-05 Identities = 25/54 (46%), Positives = 30/54 (55%), Gaps = 1/54 (1%) Frame = +1 Query: 466 DLASY-LLQ*AACRPQEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQ 624 DL Y L Q P EHP+L+TE NP NR K + MFE+ PA Y+A Q Sbjct: 82 DLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMFESLRCPATYLAKQ 135 Score = 36.7 bits (81), Expect = 0.003 Identities = 23/81 (28%), Positives = 38/81 (46%) Frame = +2 Query: 266 KAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVT 445 + GF+G+D P+ V PS G + G + Y+ +S G + +K I +G V Sbjct: 24 RIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNPG-MEIKNAIRNGWVE 75 Query: 446 NWDDMEKIWHHTFYNELRVAP 508 NWD +W + +L+ P Sbjct: 76 NWDVTVDLWRYGLEQQLKTNP 96 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 42.3 bits (95), Expect = 5e-05 Identities = 21/74 (28%), Positives = 43/74 (58%), Gaps = 1/74 (1%) Frame = +2 Query: 266 KAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL-KYPIEHGIV 442 +AG+ G+ P+ VF ++V R R + + + + VG++ + G ++ + P E ++ Sbjct: 37 RAGWGGEKDPKLVFDNLVSRYRDRK----LSRTSTLVGNDTLIEVGSRSIARSPFERNVI 92 Query: 443 TNWDDMEKIWHHTF 484 +NWD ME++ +TF Sbjct: 93 SNWDLMEQVLDYTF 106 Score = 38.3 bits (85), Expect = 9e-04 Identities = 15/37 (40%), Positives = 23/37 (62%) Frame = +1 Query: 511 EHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAI 621 EHP+ +TE NP R MT+++FE +N P++ I Sbjct: 115 EHPICMTEPLANPTYVRSTMTELLFELYNAPSVAYGI 151 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 39.5 bits (88), Expect = 4e-04 Identities = 25/89 (28%), Positives = 43/89 (48%), Gaps = 4/89 (4%) Frame = +2 Query: 254 PGMC--KAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGI--LTLKY 421 PG C + G+AG+++P + PS + GV + ++ YV DE Q I + +K Sbjct: 17 PGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHAPIPGMEVKN 70 Query: 422 PIEHGIVTNWDDMEKIWHHTFYNELRVAP 508 +GI+ +W+ W +L+V P Sbjct: 71 GKSNGIIQDWESTLYTWERGLKEKLQVNP 99 Score = 34.3 bits (75), Expect = 0.015 Identities = 13/40 (32%), Positives = 25/40 (62%) Frame = +1 Query: 505 PQEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQ 624 P E+ +++TE NP++ R+++ + FE + PA Y+ Q Sbjct: 99 PTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAFYLTKQ 138 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 31.1 bits (67), Expect = 0.14 Identities = 16/48 (33%), Positives = 26/48 (54%) Frame = +2 Query: 281 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 424 G+ PRA F ++ P H G+++ M KD G+E S +G + + P Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547 >SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 732 Score = 28.7 bits (61), Expect = 0.72 Identities = 15/44 (34%), Positives = 21/44 (47%) Frame = +3 Query: 492 SCVSPPGTPSPAH*GSPQPQGQQREDDPDHVRNIQHARHVRRHP 623 S VS P PS H G+P P G+Q + N+Q + + P Sbjct: 100 SLVSTPTAPSANHHGNPFPNGKQSIKAMPSLVNLQEDSVISKFP 143 >SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizosaccharomyces pombe|chr 1|||Manual Length = 927 Score = 28.3 bits (60), Expect = 0.95 Identities = 13/30 (43%), Positives = 16/30 (53%) Frame = -3 Query: 389 VPHLLHKSPSVPYRPSRPDGGAFPRSRGTR 300 + L+ K+PS PY SRP A S TR Sbjct: 306 IDQLISKAPSYPYSSSRPSASASLASSPTR 335 >SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 277 Score = 27.1 bits (57), Expect = 2.2 Identities = 11/29 (37%), Positives = 16/29 (55%) Frame = -2 Query: 321 PTIEGNTARGASSPAKPALHIPGHCLLPT 235 P +E + +S+PA P L+ P H PT Sbjct: 107 PNVEADVKSLSSTPAAPQLNSPSHSYEPT 135 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 27.1 bits (57), Expect = 2.2 Identities = 11/32 (34%), Positives = 18/32 (56%) Frame = +2 Query: 422 PIEHGIVTNWDDMEKIWHHTFYNELRVAPRNT 517 PI+ G V +W+ ++ W H Y+ L P +T Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDT 143 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.6 bits (56), Expect = 2.9 Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 2/41 (4%) Frame = -2 Query: 381 SPT*ESFC--PIPTITP*WRGLPTIEGNTARGASSPAKPAL 265 S T +SF P+PT P LPT NT + P+ PAL Sbjct: 64 SNTPKSFAAPPVPTGAP---SLPTSSNNTQQAEERPSMPAL 101 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 3.8 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +1 Query: 523 LLTEAPLNPKANREKMTQIMFETFN 597 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|Schizosaccharomyces pombe|chr 2|||Manual Length = 2337 Score = 25.8 bits (54), Expect = 5.1 Identities = 10/27 (37%), Positives = 17/27 (62%) Frame = -1 Query: 418 FEGQDTSFALCLISYIRVLLSHTDHHA 338 +EG+DT+ +Y+R++L TD A Sbjct: 74 YEGEDTTRITRFANYLRIILPGTDQKA 100 >SPAC1F7.05 |cdc22||ribonucleoside reductase large subunit Cdc22|Schizosaccharomyces pombe|chr 1|||Manual Length = 811 Score = 25.8 bits (54), Expect = 5.1 Identities = 21/60 (35%), Positives = 29/60 (48%), Gaps = 2/60 (3%) Frame = +2 Query: 356 GQKDSYVGDEAQSKRGILTLK-YPIEHGIVTNWDDM-EKIWHHTFYNELRVAPRNTQSCS 529 G +SY G A +GIL + + + +W ++ EKI H N L VAP T S S Sbjct: 550 GTYESYEGSPAS--QGILQYDMWNVNPTDLWDWAELKEKIAKHGIRNSLLVAPMPTASTS 607 >SPCC1906.04 |wtf20||wtf element Wtf20|Schizosaccharomyces pombe|chr 3|||Manual Length = 258 Score = 25.4 bits (53), Expect = 6.7 Identities = 11/47 (23%), Positives = 23/47 (48%) Frame = +2 Query: 131 VSHG*QSLQGV*FSSCHTYILLISLIQDVRRRSCRVGSRQWPGMCKA 271 + H + + + F+ C + I + L++ + S +G +QW C A Sbjct: 151 IKHFLKKWRNMFFTFCKSPIFCLVLLKAENKLSSHLGDQQWGWKCSA 197 >SPBC16A3.11 |eso1||sister chromatid cohesion protein Eso1|Schizosaccharomyces pombe|chr 2|||Manual Length = 872 Score = 25.4 bits (53), Expect = 6.7 Identities = 12/44 (27%), Positives = 21/44 (47%) Frame = -1 Query: 376 YIRVLLSHTDHHALMAGPSHDRGEHGARSIISCETGLAHTGPLS 245 +I + +S + + + PSHD+ + +TG H PLS Sbjct: 559 HIALSISRKERYNNLVPPSHDKPKQVKPKTYGRKTGSKHYAPLS 602 >SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 564 Score = 25.0 bits (52), Expect = 8.9 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = +2 Query: 341 VMVGMGQKDSYVGDEAQSKRGILTLKYPI 427 +M+G +Y+G AQ KR +LT + I Sbjct: 165 IMIGAIHGFAYIGLAAQGKRALLTARVTI 193 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,508,169 Number of Sequences: 5004 Number of extensions: 54541 Number of successful extensions: 152 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 136 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 150 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 275671126 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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