BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10712 (751 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F ... 69 5e-13 SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF... 69 9e-13 SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase |Schizosa... 29 0.94 SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 26 6.6 SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam... 26 6.6 SPAC1805.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 6.6 >SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F complex subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 218 Score = 69.3 bits (162), Expect = 5e-13 Identities = 31/77 (40%), Positives = 44/77 (57%), Gaps = 1/77 (1%) Frame = +1 Query: 280 DYAVFKQGIRPMWEDDANKMGGRWLISLEKKQRFTDLDRFWLDVVLLLIGENFE-NSDEI 456 DY+ F++G+RP WED NK GG+W K + LD WL VL IGE + E+ Sbjct: 92 DYSFFREGVRPEWEDVHNKTGGKWAFQ-NKGRGGNALDEMWLTTVLAAIGETLDPTGQEV 150 Query: 457 CGAVVNVRPKVDKIAIW 507 G V+N+R ++A+W Sbjct: 151 MGVVINMRKGFYRLAVW 167 Score = 43.2 bits (97), Expect = 4e-05 Identities = 18/48 (37%), Positives = 28/48 (58%), Gaps = 1/48 (2%) Frame = +2 Query: 110 FLIKHPLQNQWSLWF-YDNDRNKTWEENLIELTTFDTVEDFWRLYHHI 250 F +KHPL W+LWF W E + TF++VE+FW ++++I Sbjct: 34 FNLKHPLARPWTLWFLMPPTPGLEWNELQKNIITFNSVEEFWGIHNNI 81 >SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF4E 4F complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 243 Score = 68.5 bits (160), Expect = 9e-13 Identities = 32/77 (41%), Positives = 46/77 (59%), Gaps = 1/77 (1%) Frame = +1 Query: 280 DYAVFKQGIRPMWEDDANKMGGRWLISLEKKQRFTDLDRFWLDVVLLLIGENFE-NSDEI 456 DY+ F +GIRP WED N GG+W + + K + ++LD WL +VL IGE + E+ Sbjct: 118 DYSYFLKGIRPEWEDPQNMNGGKW--AYQSKHKGSNLDELWLYMVLAAIGETLDPTGKEV 175 Query: 457 CGAVVNVRPKVDKIAIW 507 G V N+R +IA+W Sbjct: 176 TGVVCNMRKGFYRIAVW 192 Score = 46.8 bits (106), Expect = 3e-06 Identities = 22/55 (40%), Positives = 33/55 (60%), Gaps = 1/55 (1%) Frame = +2 Query: 89 NAEVPPEFLIKHPLQNQWSLWFY-DNDRNKTWEENLIELTTFDTVEDFWRLYHHI 250 NAE F+ HPLQ++W+LWF + W + L E+ +F TVE+FW ++ I Sbjct: 55 NAETA--FVKTHPLQHEWTLWFLKPPTQGLEWSDLLKEIISFKTVEEFWGIFKTI 107 >SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase |Schizosaccharomyces pombe|chr 1|||Manual Length = 783 Score = 28.7 bits (61), Expect = 0.94 Identities = 16/34 (47%), Positives = 21/34 (61%), Gaps = 2/34 (5%) Frame = +2 Query: 572 DTRQDW-FPSTQRH-NG*A*FCDQESIHCLVLAL 667 D Q+W FP TQ++ N FC+Q H L+LAL Sbjct: 98 DAMQNWIFPQTQQYRNYQKEFCEQALFHNLLLAL 131 >SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 2685 Score = 25.8 bits (54), Expect = 6.6 Identities = 13/34 (38%), Positives = 18/34 (52%) Frame = +2 Query: 110 FLIKHPLQNQWSLWFYDNDRNKTWEENLIELTTF 211 F I+ L+ + FY N +NK W N +L TF Sbjct: 511 FEIESSLRLSSNKVFYHNPKNKKWLINTSDLITF 544 >SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1082 Score = 25.8 bits (54), Expect = 6.6 Identities = 11/22 (50%), Positives = 14/22 (63%) Frame = +3 Query: 117 SSTRYKTSGVSGFTTMTETKHG 182 ++T Y TSG GFTT T+ G Sbjct: 647 TTTEYTTSGSVGFTTTLATQSG 668 >SPAC1805.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 527 Score = 25.8 bits (54), Expect = 6.6 Identities = 13/38 (34%), Positives = 20/38 (52%), Gaps = 1/38 (2%) Frame = +1 Query: 304 IRPMWEDDANKMGGRWLIS-LEKKQRFTDLDRFWLDVV 414 + +WE N G +L +EKK F +DR+W V+ Sbjct: 161 VAALWEQ-FNIFHGSYLFDEIEKKSSFEIIDRWWTSVL 197 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,047,517 Number of Sequences: 5004 Number of extensions: 61826 Number of successful extensions: 180 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 165 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 174 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 357280532 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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