BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV10703
(660 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1006.07 |||translation initiation factor eIF4A|Schizosacchar... 29 0.59
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 27 1.8
SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces pom... 27 3.2
SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 7.3
SPBC3D6.16 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 7.3
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 25 7.3
SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyce... 25 7.3
SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual 25 7.3
SPAC15A10.10 |mde6||Muskelin homolog|Schizosaccharomyces pombe|c... 25 9.7
SPBC800.10c |||EPS15 repeat family actin cortical patch componen... 25 9.7
SPCC4B3.15 |mid1|dmf1|medial ring protein Mid1|Schizosaccharomyc... 25 9.7
>SPAC1006.07 |||translation initiation factor
eIF4A|Schizosaccharomyces pombe|chr 1|||Manual
Length = 392
Score = 29.1 bits (62), Expect = 0.59
Identities = 10/26 (38%), Positives = 18/26 (69%)
Frame = +3
Query: 246 GVMAAAIDLLTNDECRLLLEVEDFFN 323
G +I+ +TND+ R++ E+E F+N
Sbjct: 353 GRKGVSINFVTNDDVRMMREIEQFYN 378
>SPAC1834.01 |sup45||translation release factor
eRF1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 433
Score = 27.5 bits (58), Expect = 1.8
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = +1
Query: 394 STRHHRRS*NQVPPNSLTIFCSS*TVNANK 483
STR + N+VP N L I+C + NK
Sbjct: 74 STRERLKLYNKVPDNGLVIYCGEVIMEGNK 103
>SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 396
Score = 26.6 bits (56), Expect = 3.2
Identities = 25/81 (30%), Positives = 35/81 (43%), Gaps = 2/81 (2%)
Frame = +3
Query: 24 FSCNFAITMGF*YLIVCTPACAFTHN-IKMLAKMAPS-QDKLVHLHKTPTSLDINPSGLL 197
F C I++ F I A A H I L + P +D H K I+P LL
Sbjct: 310 FVCPSCISLDFDLQI---QAFARQHRVISTLGVVYPEREDSCFHKIKAAQWHQISPRSLL 366
Query: 198 FAFVELNHYNNECESEGVMAA 260
F F E NH +++ ++AA
Sbjct: 367 FQFQEQNHIHHKKIRRKLLAA 387
>SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 583
Score = 25.4 bits (53), Expect = 7.3
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +3
Query: 279 NDECRLLLEVEDFFNDDC 332
NDECR L + FF+ +C
Sbjct: 535 NDECRRLKQCNHFFHREC 552
>SPBC3D6.16 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 95
Score = 25.4 bits (53), Expect = 7.3
Identities = 12/29 (41%), Positives = 14/29 (48%)
Frame = +3
Query: 135 DKLVHLHKTPTSLDINPSGLLFAFVELNH 221
D + H K +S NPS LLF L H
Sbjct: 59 DGIPHSRKKVSSAHFNPSTLLFLLKRLGH 87
>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1828
Score = 25.4 bits (53), Expect = 7.3
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = +3
Query: 219 HYNNECESEGVMAAAIDLLTNDECRLLLEVEDF 317
+Y EC S + A LTNDE LL +++F
Sbjct: 556 NYARECISLSIKIAVSHKLTNDETYLLKILKNF 588
>SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2310
Score = 25.4 bits (53), Expect = 7.3
Identities = 14/49 (28%), Positives = 23/49 (46%)
Frame = -2
Query: 404 WRVETRSVIDFDLRCSARKVFKQITIIIEEILNFEQQTALIVSEQIDGR 258
W T+ V R S RKV + + + EE +++ ALI + + R
Sbjct: 2143 WSTPTKLVEPSQFRASPRKVDQAVVLSSEEKEILQKKYALIAEDNLIAR 2191
>SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual
Length = 304
Score = 25.4 bits (53), Expect = 7.3
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 4/49 (8%)
Frame = +3
Query: 111 LAKMAPSQDKLVHLH----KTPTSLDINPSGLLFAFVELNHYNNECESE 245
L + ++KLV+L T T LD + ++ A V+ N Y N C+++
Sbjct: 170 LEEFESQKEKLVYLSADSDNTITELDEDKIYIIGAIVDKNRYKNLCQNK 218
>SPAC15A10.10 |mde6||Muskelin homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 716
Score = 25.0 bits (52), Expect = 9.7
Identities = 13/26 (50%), Positives = 15/26 (57%), Gaps = 2/26 (7%)
Frame = +3
Query: 480 QGNIFSNWLEKKLTSLRSSK--TFLK 551
Q I W + KLT LRS + TFLK
Sbjct: 233 QAEIIEKWPKHKLTKLRSHRIATFLK 258
>SPBC800.10c |||EPS15 repeat family actin cortical patch component
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1116
Score = 25.0 bits (52), Expect = 9.7
Identities = 15/45 (33%), Positives = 25/45 (55%), Gaps = 1/45 (2%)
Frame = +3
Query: 156 KTPTSLDINPSGLLFA-FVELNHYNNECESEGVMAAAIDLLTNDE 287
K P DI+ SG+ + FV+LN NN +G + + +++DE
Sbjct: 90 KIPYFADIHISGVDSSKFVQLNRPNNVSSGDGSDGSFLPPISSDE 134
>SPCC4B3.15 |mid1|dmf1|medial ring protein Mid1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 920
Score = 25.0 bits (52), Expect = 9.7
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +2
Query: 539 NISEVPERVDPQPPAAVLASS 601
N++ +P V P PPA + ASS
Sbjct: 221 NLARIPSDVKPVPPAHLSASS 241
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,599,847
Number of Sequences: 5004
Number of extensions: 50891
Number of successful extensions: 144
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 144
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 299817502
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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