SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV10697X
         (450 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g37475.1 68418.m04510 translation initiation factor-related s...    46   2e-05
At1g66070.1 68414.m07499 translation initiation factor-related s...    37   0.005
At5g25590.1 68418.m03045 expressed protein contains Pfam profile...    28   2.5  
At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P329...    28   2.5  
At3g21160.1 68416.m02673 mannosyl-oligosaccharide 1,2-alpha-mann...    28   2.5  
At4g14480.1 68417.m02233 protein kinase family protein contains ...    28   3.4  
At1g61840.1 68414.m06978 DC1 domain-containing protein similar t...    28   3.4  
At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P460...    27   4.4  
At1g73440.1 68414.m08501 calmodulin-related low similarity to ca...    27   4.4  
At1g51590.1 68414.m05808 mannosyl-oligosaccharide 1,2-alpha-mann...    27   4.4  
At5g44280.1 68418.m05420 zinc finger (C3HC4-type RING finger) fa...    27   5.9  
At5g19630.1 68418.m02336 expressed protein                             27   5.9  
At5g11140.1 68418.m01302 hypothetical protein                          27   5.9  
At5g04560.1 68418.m00456 DEMETER protein (DME) identical to DEME...    27   5.9  
At3g50590.1 68416.m05533 transducin family protein / WD-40 repea...    27   5.9  
At3g45555.1 68416.m04920 zinc finger (C3HC4-type RING finger) fa...    27   5.9  
At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P329...    27   5.9  
At5g56950.1 68418.m07109 nucleosome assembly protein (NAP), puta...    27   7.7  
At5g35794.1 68418.m04297 hypothetical protein contains Pfam prof...    27   7.7  
At4g17940.1 68417.m02672 expressed protein                             27   7.7  
At3g45510.1 68416.m04914 zinc finger (C3HC4-type RING finger) fa...    27   7.7  
At3g19810.1 68416.m02509 expressed protein contains Pfam PF02620...    27   7.7  
At3g17170.1 68416.m02190 ribosomal protein S6 family protein (RF...    27   7.7  
At3g16180.1 68416.m02043 proton-dependent oligopeptide transport...    27   7.7  
At2g40540.1 68415.m05002 potassium transporter, putative (KT2) i...    27   7.7  
At1g56510.1 68414.m06498 disease resistance protein (TIR-NBS-LRR...    27   7.7  
At1g03010.1 68414.m00273 phototropic-responsive NPH3 family prot...    27   7.7  

>At5g37475.1 68418.m04510 translation initiation factor-related
           similar to Eukaryotic translation initiation factor 3
           subunit 1 (eIF-3 alpha) (eIF3 p35) (eIF3j)
           (Swiss-Prot:O75822) [Homo sapiens]
          Length = 225

 Score = 45.6 bits (103), Expect = 2e-05
 Identities = 17/40 (42%), Positives = 31/40 (77%), Gaps = 1/40 (2%)
 Frame = +3

Query: 288 WDADNFEPKLPTTLAASNKWEGEDEDDN-VKESWEDEEVA 404
           W+A++F+P LP+ +   + W+ ED D+N +K+SWE+E+V+
Sbjct: 4   WEAEDFQP-LPSKVELKSNWDDEDVDENDIKDSWEEEDVS 42


>At1g66070.1 68414.m07499 translation initiation factor-related
           similar to Eukaryotic translation initiation factor 3
           subunit 1 (eIF-3 alpha) (eIF3 p35) (eIF3j)
           (Swiss-Prot:O75822) [Homo sapiens]
          Length = 226

 Score = 37.1 bits (82), Expect = 0.005
 Identities = 14/38 (36%), Positives = 24/38 (63%), Gaps = 1/38 (2%)
 Frame = +3

Query: 288 WDADNFEPKLPTTLAASNKWEGEDEDDN-VKESWEDEE 398
           W+ +   P LP  +   + W+ ED D+N +K+SWED++
Sbjct: 4   WEDEKIAP-LPAKVELKSNWDDEDVDENEIKDSWEDDD 40


>At5g25590.1 68418.m03045 expressed protein contains Pfam profile
           PF04783: Protein of unknown function (DUF630)
          Length = 775

 Score = 28.3 bits (60), Expect = 2.5
 Identities = 13/42 (30%), Positives = 24/42 (57%)
 Frame = +3

Query: 297 DNFEPKLPTTLAASNKWEGEDEDDNVKESWEDEEVAKNRRRK 422
           +  EPK P  +    + E E+ED+  +E  E+E V + +++K
Sbjct: 258 EEMEPKTPEKVEEEEE-EDEEEDEEEEEEEEEEVVVEVKKKK 298


>At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P32962
           Nitrilase 2 (EC 3.5.5.1) {Arabidopsis thaliana}
          Length = 339

 Score = 28.3 bits (60), Expect = 2.5
 Identities = 12/22 (54%), Positives = 15/22 (68%)
 Frame = -2

Query: 365 ILVFPFPFVGGCQRGREFGLEV 300
           ++VFP  F+GG  RG  FGL V
Sbjct: 53  LVVFPEAFIGGYPRGFRFGLGV 74


>At3g21160.1 68416.m02673 mannosyl-oligosaccharide
           1,2-alpha-mannosidase, putative similar to
           mannosyl-oligosaccharide 1,2-alpha-mannosidase [Glycine
           max][GI:6552504]
          Length = 572

 Score = 28.3 bits (60), Expect = 2.5
 Identities = 13/39 (33%), Positives = 18/39 (46%)
 Frame = +3

Query: 270 LKMDVSWDADNFEPKLPTTLAASNKWEGEDEDDNVKESW 386
           L  +++W   NF    PT LA  N +     D +V  SW
Sbjct: 402 LAEELAWTCYNFYQSTPTKLAGENYFFNSGSDMSVGTSW 440


>At4g14480.1 68417.m02233 protein kinase family protein contains
           protein kinase domain, Pfam:PF00069
          Length = 487

 Score = 27.9 bits (59), Expect = 3.4
 Identities = 11/18 (61%), Positives = 14/18 (77%)
 Frame = +3

Query: 363 DDNVKESWEDEEVAKNRR 416
           DD+ +E  EDEE+ KNRR
Sbjct: 329 DDDEEEEEEDEEIVKNRR 346


>At1g61840.1 68414.m06978 DC1 domain-containing protein similar to
           hypothetical protein GI:3184279 from [Arabidopsis
           thaliana]; contains Pfam profile PF03107: DC1 domain
          Length = 814

 Score = 27.9 bits (59), Expect = 3.4
 Identities = 10/25 (40%), Positives = 17/25 (68%)
 Frame = +3

Query: 186 SDKSLSIVVRFWCSGEKFSY*FGAD 260
           SD+ LS++  FWC+ ++F+   G D
Sbjct: 278 SDEDLSVLPLFWCNNKEFNVDGGCD 302


>At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P46010
           Nitrilase 3 (EC 3.5.5.1) {Arabidopsis thaliana}
          Length = 346

 Score = 27.5 bits (58), Expect = 4.4
 Identities = 11/22 (50%), Positives = 15/22 (68%)
 Frame = -2

Query: 365 ILVFPFPFVGGCQRGREFGLEV 300
           +++FP  F+GG  RG  FGL V
Sbjct: 60  LVLFPEAFIGGYPRGFRFGLAV 81


>At1g73440.1 68414.m08501 calmodulin-related low similarity to
           calmodulin 8 [Arabidopsis thaliana] GI:5825600; contains
           Pfam profiles PF02809: Ubiquitin interaction motif,
           PF00036: EF hand
          Length = 254

 Score = 27.5 bits (58), Expect = 4.4
 Identities = 11/25 (44%), Positives = 17/25 (68%)
 Frame = +3

Query: 348 EGEDEDDNVKESWEDEEVAKNRRRK 422
           +G+++DD V+E  EDEE    R+ K
Sbjct: 94  DGDEDDDEVEEIDEDEEFLCKRKNK 118


>At1g51590.1 68414.m05808 mannosyl-oligosaccharide
           1,2-alpha-mannosidase, putative similar to
           mannosyl-oligosaccharide 1,2-alpha-mannosidase [Glycine
           max][GI:6552504]
          Length = 560

 Score = 27.5 bits (58), Expect = 4.4
 Identities = 12/36 (33%), Positives = 18/36 (50%)
 Frame = +3

Query: 279 DVSWDADNFEPKLPTTLAASNKWEGEDEDDNVKESW 386
           +++W   NF    PT LA  N +    +D +V  SW
Sbjct: 404 ELAWTCYNFYQSTPTKLAGENYFFTAGQDMSVGTSW 439


>At5g44280.1 68418.m05420 zinc finger (C3HC4-type RING finger)
           family protein contains similarity to polycomb-M33
           interacting protein Ring1B [Mus musculus] GI:2239142;
           contains Pfam profile PF00097: Zinc finger, C3HC4 type
           (RING finger)
          Length = 486

 Score = 27.1 bits (57), Expect = 5.9
 Identities = 15/51 (29%), Positives = 23/51 (45%)
 Frame = +3

Query: 291 DADNFEPKLPTTLAASNKWEGEDEDDNVKESWEDEEVAKNRRRKD*GSDTS 443
           D D+ E      + A    E E+E++  +E  E+EE +K R       D S
Sbjct: 70  DGDDAEEDEEEEVEAEEDEEAEEEEEEEEEEEEEEEDSKERSPSSISGDQS 120


>At5g19630.1 68418.m02336 expressed protein
          Length = 228

 Score = 27.1 bits (57), Expect = 5.9
 Identities = 10/15 (66%), Positives = 12/15 (80%)
 Frame = -2

Query: 371 VIILVFPFPFVGGCQ 327
           VI+LV PF  +GGCQ
Sbjct: 41  VIVLVHPFSLLGGCQ 55


>At5g11140.1 68418.m01302 hypothetical protein
          Length = 241

 Score = 27.1 bits (57), Expect = 5.9
 Identities = 12/30 (40%), Positives = 20/30 (66%)
 Frame = -1

Query: 225 NTKIGQLSTVTYHSQDVSRVVVF*TTTKLK 136
           N+K+  LST TYH + ++ VV+   +T L+
Sbjct: 85  NSKLQSLSTRTYHLETLAEVVIELQSTPLR 114


>At5g04560.1 68418.m00456 DEMETER protein (DME) identical to DEMETER
           protein [Arabidopsis thaliana] GI:21743571; contains
           Pfam profile PF00730: HhH-GPD superfamily base excision
           DNA repair protein
          Length = 1729

 Score = 27.1 bits (57), Expect = 5.9
 Identities = 12/26 (46%), Positives = 15/26 (57%)
 Frame = +3

Query: 348 EGEDEDDNVKESWEDEEVAKNRRRKD 425
           EG++E D  KE W +EE    R R D
Sbjct: 719 EGDEEKDKKKEKWWEEERRVFRGRAD 744


>At3g50590.1 68416.m05533 transducin family protein / WD-40 repeat
            family protein contains 3 WD-40 repeats (PF00400); some
            similarity to s-tomosyn isoform (GI:4689231)[Rattus
            norvegicus]; contains non-consensus AT-AC splice sites at
            intron 18
          Length = 1606

 Score = 27.1 bits (57), Expect = 5.9
 Identities = 14/34 (41%), Positives = 18/34 (52%)
 Frame = +3

Query: 249  FGADSGVLKMDVSWDADNFEPKLPTTLAASNKWE 350
            F A+S V K    W   N+E K PT + +  KWE
Sbjct: 992  FAAESMVPK-GPEWGGGNWEIKTPTDMKSIPKWE 1024


>At3g45555.1 68416.m04920 zinc finger (C3HC4-type RING finger)
           family protein low similarity to UbcM4-interacting
           protein 4 [Mus musculus] GI:13991706; contains Pfam
           profiles PF00097: Zinc finger C3HC4 type (RING finger),
           PF01485: IBR (In Between Ring fingers) domain
          Length = 213

 Score = 27.1 bits (57), Expect = 5.9
 Identities = 8/33 (24%), Positives = 19/33 (57%)
 Frame = +3

Query: 144 LLLSRKRRLWRHLVSDKSLSIVVRFWCSGEKFS 242
           +L S+ + +W   + ++S+ +  RF+C   + S
Sbjct: 78  ILTSKLKAMWEQKIEEESIPVTERFYCPNPRCS 110


>At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P32961
           Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
          Length = 346

 Score = 27.1 bits (57), Expect = 5.9
 Identities = 11/22 (50%), Positives = 15/22 (68%)
 Frame = -2

Query: 365 ILVFPFPFVGGCQRGREFGLEV 300
           +++FP  F+GG  RG  FGL V
Sbjct: 60  LVLFPEGFIGGYPRGFRFGLAV 81


>At5g56950.1 68418.m07109 nucleosome assembly protein (NAP),
           putative similar to nucleosome assembly protein 1
           [Glycine max] GI:1161252; contains Pfam profile PF00956:
           Nucleosome assembly protein (NAP)
          Length = 374

 Score = 26.6 bits (56), Expect = 7.7
 Identities = 10/24 (41%), Positives = 17/24 (70%)
 Frame = +3

Query: 348 EGEDEDDNVKESWEDEEVAKNRRR 419
           E EDED+   +  E+EEV+K +++
Sbjct: 323 EDEDEDEEEDDEDEEEEVSKTKKK 346


>At5g35794.1 68418.m04297 hypothetical protein contains Pfam profile
           PF03384: Drosophila protein of unknown function, DUF287
          Length = 662

 Score = 26.6 bits (56), Expect = 7.7
 Identities = 17/46 (36%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
 Frame = +3

Query: 315 LPTTLAASNKWEGEDEDDNVK-ESWEDEEVAKNRRRKD*GSDTSTT 449
           +PT     N    E ED+N K E+ E+EE  K+ R     SD+  T
Sbjct: 89  IPTEEIQENMQTDEVEDENEKEEASEEEESGKSSRTLGSDSDSEET 134


>At4g17940.1 68417.m02672 expressed protein
          Length = 274

 Score = 26.6 bits (56), Expect = 7.7
 Identities = 10/20 (50%), Positives = 15/20 (75%)
 Frame = +3

Query: 345 WEGEDEDDNVKESWEDEEVA 404
           WE ED+DD+ +E  E+E +A
Sbjct: 246 WEAEDDDDDDEEEEEEEWMA 265


>At3g45510.1 68416.m04914 zinc finger (C3HC4-type RING finger)
           family protein contains a zinc finger, C3HC4 type (RING
           finger), signature, PROSITE:PS00518
          Length = 257

 Score = 26.6 bits (56), Expect = 7.7
 Identities = 9/31 (29%), Positives = 17/31 (54%)
 Frame = +3

Query: 144 LLLSRKRRLWRHLVSDKSLSIVVRFWCSGEK 236
           LL  ++R +W   + ++S+ +  RF C   K
Sbjct: 127 LLTPKQREMWEQRIKEESIPVCNRFHCPNPK 157


>At3g19810.1 68416.m02509 expressed protein contains Pfam PF02620:
           Uncharacterized ACR, COG1399
          Length = 321

 Score = 26.6 bits (56), Expect = 7.7
 Identities = 12/21 (57%), Positives = 16/21 (76%)
 Frame = +3

Query: 336 SNKWEGEDEDDNVKESWEDEE 398
           ++K EGED+DDN  +SW D E
Sbjct: 221 NDKEEGEDDDDN-DDSWIDWE 240


>At3g17170.1 68416.m02190 ribosomal protein S6 family protein (RFC3)
           annotation temporarily based on supporting cDNA
           gi|15620809|dbj|AB057424.1|; contains TIGRfam TIGR00166
           and Pfam PF01250 profiles ribosomal protein S6.
          Length = 314

 Score = 26.6 bits (56), Expect = 7.7
 Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 5/49 (10%)
 Frame = +3

Query: 291 DADNFEPKLP-----TTLAASNKWEGEDEDDNVKESWEDEEVAKNRRRK 422
           DADN E ++        +   ++ EGE+E+D   E  E ++ + N RR+
Sbjct: 257 DADNIEYEVDDDGNVVMVLYGDEEEGEEEEDGASEQEEGQDKSTNGRRE 305


>At3g16180.1 68416.m02043 proton-dependent oligopeptide transport
           (POT) family protein contains Pfam profile: PF00854 POT
           family
          Length = 591

 Score = 26.6 bits (56), Expect = 7.7
 Identities = 14/32 (43%), Positives = 19/32 (59%)
 Frame = +2

Query: 2   WILTFLNQFVNIRLLGLRQYYVICSLFFNPTV 97
           W+L  L+ FVN+       YYV+CS  + PTV
Sbjct: 545 WVLAILS-FVNVI------YYVVCSWSYGPTV 569


>At2g40540.1 68415.m05002 potassium transporter, putative (KT2)
           identical to putative potassium transporter AtKT2p
           [Arabidopsis thaliana] gi|2384671|gb|AAC49845, strong
           similarity to potassium transporter HAK2p
           [Mesembryanthemum crystallinum] GI:14091471; KUP/HAK/KT
           Transporter family member, PMID:11500563
          Length = 794

 Score = 26.6 bits (56), Expect = 7.7
 Identities = 15/49 (30%), Positives = 27/49 (55%), Gaps = 4/49 (8%)
 Frame = +3

Query: 279 DVSWDADNFEPKLPTTLAASNKWE----GEDEDDNVKESWEDEEVAKNR 413
           DV  D D+FE +L + LA   +++     + EDDN +    +E  +++R
Sbjct: 602 DVHQDVDSFETELVSKLADFIRYDWHKRTQQEDDNARSVQSNESSSESR 650


>At1g56510.1 68414.m06498 disease resistance protein (TIR-NBS-LRR
           class), putative domain signature TIR-NBS-LRR exists,
           suggestive of a disease resistance protein.
          Length = 1007

 Score = 26.6 bits (56), Expect = 7.7
 Identities = 13/46 (28%), Positives = 25/46 (54%)
 Frame = +3

Query: 288 WDADNFEPKLPTTLAASNKWEGEDEDDNVKESWEDEEVAKNRRRKD 425
           WDA + + +LP++  A N  E   +D  +++ WE  +   N ++ D
Sbjct: 586 WDAYSGK-RLPSSFFAENLVEVNMQDSELQKLWEGTQCLANLKKID 630


>At1g03010.1 68414.m00273 phototropic-responsive NPH3 family protein
           contains NPH3 family domain, Pfam:PF03000
          Length = 634

 Score = 26.6 bits (56), Expect = 7.7
 Identities = 10/20 (50%), Positives = 14/20 (70%)
 Frame = +3

Query: 261 SGVLKMDVSWDADNFEPKLP 320
           SG+LK+D S+   N EP+ P
Sbjct: 184 SGLLKLDYSFSGTNIEPQTP 203


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,052,949
Number of Sequences: 28952
Number of extensions: 208518
Number of successful extensions: 873
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 772
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 857
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 732537840
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -