BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10688 (741 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 27 2.1 SPAC2E1P3.02c |amt3||ammonium transporter Amt3|Schizosaccharomyc... 27 3.7 SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe... 27 3.7 SPCC777.13 |vps35||retromer complex subunit Vps35|Schizosaccharo... 27 3.7 >SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 3655 Score = 27.5 bits (58), Expect = 2.1 Identities = 21/81 (25%), Positives = 36/81 (44%), Gaps = 9/81 (11%) Frame = +1 Query: 304 LLESYRVLTYCGNLDQMMPCV---------VTSEHYRTWKWSSSDDFIKAVRNPYMFKNR 456 +L++YR + G + ++PC + Y + SS DFI+ +RN Y ++N Sbjct: 220 ILQAYRQAAFPG-VQAIIPCFLKMVQIDVPIDIASYAMIEKDSSIDFIEFIRNKYQYRNF 278 Query: 457 LAGYHKTGGNLTELIIREPVT 519 KT L ++ P T Sbjct: 279 FMAQVKTLSFLAYILRTHPNT 299 >SPAC2E1P3.02c |amt3||ammonium transporter Amt3|Schizosaccharomyces pombe|chr 1|||Manual Length = 517 Score = 26.6 bits (56), Expect = 3.7 Identities = 11/42 (26%), Positives = 21/42 (50%) Frame = +1 Query: 226 IHVRERYRESKMAPDFLSSSKALYEELLESYRVLTYCGNLDQ 351 +H+R + E DF+ + L + LES ++ Y +D+ Sbjct: 460 LHIRASFEEELRGMDFVELEEQLPGQTLESKPMIIYAQEMDE 501 >SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 972 Score = 26.6 bits (56), Expect = 3.7 Identities = 14/53 (26%), Positives = 28/53 (52%), Gaps = 1/53 (1%) Frame = +1 Query: 259 MAPDFLSSSKALYEELLESYRVLTYCGNLDQMMPCV-VTSEHYRTWKWSSSDD 414 M + S + LY L+ Y + T+C ++D M CV + + + ++ W +S + Sbjct: 716 MLENCASKEELLYVCLI--YTIWTHCTDMDSMDNCVYLCIQKFESYGWGASSE 766 >SPCC777.13 |vps35||retromer complex subunit Vps35|Schizosaccharomyces pombe|chr 3|||Manual Length = 785 Score = 26.6 bits (56), Expect = 3.7 Identities = 18/69 (26%), Positives = 35/69 (50%), Gaps = 1/69 (1%) Frame = +2 Query: 8 AIQPLLTALSDDIAANRSVEAKNRFMGLVSLLLYLSHQTHAYN-FLEDRVVLENHEKFLN 184 AIQ +L + ++ +RS + + ++S +L + + Y DRV +E +N Sbjct: 308 AIQEVLWSHVVEVIQSRSGLPLDCIVSILSSILNFFLRCYPYKPQYADRVFQYINEHIIN 367 Query: 185 KPEIRKAIH 211 +P +R A+H Sbjct: 368 QPSLRSALH 376 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,885,784 Number of Sequences: 5004 Number of extensions: 59024 Number of successful extensions: 170 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 167 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 170 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 351258950 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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