Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV10672
(793 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 155 9e-39
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 152 5e-38
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 87 4e-18
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 58 1e-09
SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein 2|S... 30 0.33
SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|ch... 27 4.1
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 155 bits (375), Expect = 9e-39
Identities = 71/91 (78%), Positives = 78/91 (85%)
Frame = +2
Query: 506 SIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHF 685
S YDICRRNLDIERP+Y NLNRLI Q+VSSITASLRF+G+LNVDL EFQTNLVPYPRIHF
Sbjct: 208 SCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHF 267
Query: 686 PLVTYAPVISAEKAYHEQLSVAEITKHASSP 778
PLVTYAP++SA KA+HE SV EIT P
Sbjct: 268 PLVTYAPIVSAAKAFHESNSVQEITNQCFEP 298
Score = 138 bits (333), Expect = 1e-33
Identities = 59/78 (75%), Positives = 70/78 (89%)
Frame = +1
Query: 22 DDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYAR 201
D F+TFFSETG GK+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYAR
Sbjct: 46 DGGFSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYAR 105
Query: 202 GHYTIGKEIVDLVLDRIR 255
GHYT+GKE+VD V D+IR
Sbjct: 106 GHYTVGKELVDEVTDKIR 123
Score = 121 bits (291), Expect = 1e-28
Identities = 52/85 (61%), Positives = 68/85 (80%)
Frame = +3
Query: 255 KLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVE 434
++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+VVE
Sbjct: 124 RIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVE 183
Query: 435 PYNSILTTHTTLEHSDCAFMVDNEA 509
PYNS+LTTH TL+ +DC FMVDNE+
Sbjct: 184 PYNSVLTTHATLDLADCTFMVDNES 208
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 152 bits (369), Expect = 5e-38
Identities = 70/89 (78%), Positives = 76/89 (85%)
Frame = +2
Query: 512 YDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPL 691
YDICRRNLDIERPTY NLNRLI Q+VSSITASLRF G+LNVDL EFQTNLVPYPRIHFPL
Sbjct: 214 YDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPL 273
Query: 692 VTYAPVISAEKAYHEQLSVAEITKHASSP 778
VTY+P++SA KA+HE SV EIT P
Sbjct: 274 VTYSPIVSAAKAFHESNSVQEITNQCFEP 302
Score = 136 bits (328), Expect = 4e-33
Identities = 56/78 (71%), Positives = 70/78 (89%)
Frame = +1
Query: 22 DDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYAR 201
+D F TFFSETG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYAR
Sbjct: 50 NDGFGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYAR 109
Query: 202 GHYTIGKEIVDLVLDRIR 255
GHYT+GKE++D VL+RIR
Sbjct: 110 GHYTVGKEMIDSVLERIR 127
Score = 126 bits (304), Expect = 4e-30
Identities = 54/85 (63%), Positives = 69/85 (81%)
Frame = +3
Query: 255 KLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVE 434
++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAPQVST+VVE
Sbjct: 128 RMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVE 187
Query: 435 PYNSILTTHTTLEHSDCAFMVDNEA 509
PYNS+LTTH TL++SDC FMVDNEA
Sbjct: 188 PYNSVLTTHATLDNSDCTFMVDNEA 212
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 86.6 bits (205), Expect = 4e-18
Identities = 37/77 (48%), Positives = 52/77 (67%)
Frame = +1
Query: 25 DSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARG 204
+ N +F+E GK+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+G
Sbjct: 45 ERLNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKG 104
Query: 205 HYTIGKEIVDLVLDRIR 255
HYT G E+ D VLD +R
Sbjct: 105 HYTEGAELADAVLDVVR 121
Score = 72.9 bits (171), Expect = 5e-14
Identities = 31/86 (36%), Positives = 54/86 (62%)
Frame = +2
Query: 506 SIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHF 685
++ I L I+ P+Y +LN L+ +++ +T S RF G LN DL + N+VP+PR+HF
Sbjct: 206 ALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHF 265
Query: 686 PLVTYAPVISAEKAYHEQLSVAEITK 763
+V +AP+ + + + +SV E+T+
Sbjct: 266 FMVGFAPLAAIGSSSFQAVSVPELTQ 291
Score = 66.9 bits (156), Expect = 3e-12
Identities = 31/83 (37%), Positives = 46/83 (55%)
Frame = +3
Query: 261 ADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPY 440
A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S VVEPY
Sbjct: 124 AEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPY 183
Query: 441 NSILTTHTTLEHSDCAFMVDNEA 509
N+ L+ H +E+SD F +DNEA
Sbjct: 184 NATLSMHQLVENSDETFCIDNEA 206
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 58.0 bits (134), Expect = 1e-09
Identities = 28/80 (35%), Positives = 49/80 (61%), Gaps = 2/80 (2%)
Frame = +1
Query: 19 GDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANN 192
G D + FF ++ +++PRA+ +DLEP VV+ + + TY L++PE ++ K A NN
Sbjct: 44 GVDRKDVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNN 103
Query: 193 YARGHYTIGKEIVDLVLDRI 252
+A G Y+ + I + ++D I
Sbjct: 104 WANG-YSHAERIFEDIMDMI 122
Score = 53.6 bits (123), Expect = 3e-08
Identities = 23/63 (36%), Positives = 36/63 (57%)
Frame = +2
Query: 518 ICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVT 697
I L + PT+ N+L+ ++S+ T +LR+ G +N DL +L+P PR HF L +
Sbjct: 213 IAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTS 272
Query: 698 YAP 706
Y P
Sbjct: 273 YTP 275
Score = 50.0 bits (114), Expect = 4e-07
Identities = 30/90 (33%), Positives = 44/90 (48%), Gaps = 1/90 (1%)
Frame = +3
Query: 261 ADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEP 437
AD L+GF + H L+ERL+ Y KK ++++P Q VS VV+P
Sbjct: 126 ADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQP 185
Query: 438 YNSILTTHTTLEHSDCAFMVDNEASMTSAA 527
YNS+L ++D ++DN A AA
Sbjct: 186 YNSLLALKRLTLNADSVVVLDNAALAHIAA 215
>SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein
2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 513
Score = 30.3 bits (65), Expect = 0.33
Identities = 15/51 (29%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
Frame = +3
Query: 366 YGKKSKLEFAIYPAPQVSTAVVE-PYNSILTTHTTLEHSDCAFMVDNEASM 515
Y + K+E YP ++ V + PYN +L ++LE ++DN S+
Sbjct: 110 YNSRWKIEIPSYPVREIPYVVADTPYNKLLCPLSSLEWVSKLILMDNANSI 160
>SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 425
Score = 26.6 bits (56), Expect = 4.1
Identities = 17/48 (35%), Positives = 23/48 (47%)
Frame = +1
Query: 175 EDAANNYARGHYTIGKEIVDLVLDRIRSSLTSVPVCKDS*SSTPSVEV 318
ED HY+ E++DL SS S P C D +ST ++EV
Sbjct: 223 EDVIYTCPSCHYSTNSEMLDLSKT---SSDISCPNCNDQLTSTTAIEV 267
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,244,091
Number of Sequences: 5004
Number of extensions: 65184
Number of successful extensions: 217
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 202
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 216
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 385381248
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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