BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10657X (572 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g55410.1 68416.m06154 2-oxoglutarate dehydrogenase E1 compone... 79 3e-15 At5g65750.1 68418.m08274 2-oxoglutarate dehydrogenase E1 compone... 78 4e-15 At2g16880.1 68415.m01942 pentatricopeptide (PPR) repeat-containi... 29 1.7 At5g29070.1 68418.m03617 expressed protein 29 2.9 At1g03490.1 68414.m00330 no apical meristem (NAM) family protein... 29 2.9 At1g12120.1 68414.m01404 expressed protein contains Pfam domain ... 28 3.8 At2g40190.1 68415.m04942 glycosyl transferase family 1 protein c... 28 5.1 At2g23990.2 68415.m02866 plastocyanin-like domain-containing pro... 28 5.1 At1g03310.2 68414.m00310 isoamylase, putative / starch debranchi... 27 8.9 At1g03310.1 68414.m00309 isoamylase, putative / starch debranchi... 27 8.9 >At3g55410.1 68416.m06154 2-oxoglutarate dehydrogenase E1 component, putative / oxoglutarate decarboxylase, putative / alpha-ketoglutaric dehydrogenase, putative similar to SP|P20967 2-oxoglutarate dehydrogenase E1 component, mitochondrial precursor (EC 1.2.4.2) (Alpha-ketoglutarate dehydrogenase) {Saccharomyces cerevisiae}; contains Pfam profiles PF02779: Transketolase, pyridine binding domain, PF00676: Dehydrogenase E1 component Length = 1017 Score = 78.6 bits (185), Expect = 3e-15 Identities = 41/88 (46%), Positives = 59/88 (67%), Gaps = 10/88 (11%) Frame = +1 Query: 13 FRKPLILMTPKSLLRHPECKSS---FDDM-------KEGTNFKRVIPEEGPASENPQNVR 162 FRKPLI+M PK+LLRH +CKS+ FDD+ K+GT FKR+I ++ S+ + +R Sbjct: 833 FRKPLIVMAPKNLLRHKDCKSNLSEFDDVQGHPGFDKQGTRFKRLIKDQNDHSDLEEGIR 892 Query: 163 KLAFCSGRVYYDLLKQRRDKGLEKDIAI 246 +L CSG+VYY+L +R+ G D+AI Sbjct: 893 RLVLCSGKVYYELDDERKKVG-ATDVAI 919 Score = 69.7 bits (163), Expect = 1e-12 Identities = 27/52 (51%), Positives = 39/52 (75%) Frame = +3 Query: 246 C*TEQISPFPYDQVKAEIAKYPNAQLVWSQEEHKNMGSWSYIEPRFRTLLQN 401 C EQ+ PFPYD ++ E+ +YPNA++VW QEE NMG++SYI PR T +++ Sbjct: 920 CRVEQLCPFPYDLIQRELKRYPNAEIVWCQEEAMNMGAFSYISPRLWTAMRS 971 >At5g65750.1 68418.m08274 2-oxoglutarate dehydrogenase E1 component, putative / oxoglutarate decarboxylase, putative / alpha-ketoglutaric dehydrogenase, putative similar to SP|P20967 2-oxoglutarate dehydrogenase E1 component, mitochondrial precursor (EC 1.2.4.2) (Alpha-ketoglutarate dehydrogenase) {Saccharomyces cerevisiae}; contains Pfam profiles PF02779: Transketolase, pyridine binding domain, PF00676: Dehydrogenase E1 component Length = 1025 Score = 78.2 bits (184), Expect = 4e-15 Identities = 41/88 (46%), Positives = 60/88 (68%), Gaps = 10/88 (11%) Frame = +1 Query: 13 FRKPLILMTPKSLLRHPECKSS---FDDMK-------EGTNFKRVIPEEGPASENPQNVR 162 FRKPLI+M PK+LLRH +C S+ FDD+K +GT FKR+I ++ S+ + +R Sbjct: 837 FRKPLIVMAPKNLLRHKQCVSNLSEFDDVKGHPGFDKQGTRFKRLIKDQSGHSDLEEGIR 896 Query: 163 KLAFCSGRVYYDLLKQRRDKGLEKDIAI 246 +L CSG+VYY+L ++R+ K KD+AI Sbjct: 897 RLVLCSGKVYYELDEERK-KSETKDVAI 923 Score = 65.3 bits (152), Expect = 3e-11 Identities = 27/63 (42%), Positives = 39/63 (61%) Frame = +3 Query: 210 ETRQGTRKGHCYC*TEQISPFPYDQVKAEIAKYPNAQLVWSQEEHKNMGSWSYIEPRFRT 389 E ++ K C EQ+ PFPYD ++ E+ +YPNA++VW QEE NMG + YI R T Sbjct: 912 ERKKSETKDVAICRVEQLCPFPYDLIQRELKRYPNAEIVWCQEEPMNMGGYQYIALRLCT 971 Query: 390 LLQ 398 ++ Sbjct: 972 AMK 974 >At2g16880.1 68415.m01942 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 743 Score = 29.5 bits (63), Expect = 1.7 Identities = 20/67 (29%), Positives = 29/67 (43%), Gaps = 3/67 (4%) Frame = +1 Query: 46 SLLRHPECKSSFDDMKEGTNFKRVIPEEGPASENPQNVRKLAFCSGRVY---YDLLKQRR 216 ++L + CK + + NF + EE N AFC + YDLL + Sbjct: 595 NILLNGLCKEGMTE--KALNFFNTLIEEREVDTVTYNTMISAFCKDKKLKEAYDLLSEME 652 Query: 217 DKGLEKD 237 +KGLE D Sbjct: 653 EKGLEPD 659 >At5g29070.1 68418.m03617 expressed protein Length = 307 Score = 28.7 bits (61), Expect = 2.9 Identities = 12/26 (46%), Positives = 17/26 (65%), Gaps = 1/26 (3%) Frame = +1 Query: 325 CGARRSTRTWA-RGATSSRASAPSCR 399 CG+ R WA +GAT+ S+P+CR Sbjct: 105 CGSDTQPRPWATQGATTGPESSPTCR 130 >At1g03490.1 68414.m00330 no apical meristem (NAM) family protein contains Pfam PF02365: No apical meristem (NAM) domain; Length = 253 Score = 28.7 bits (61), Expect = 2.9 Identities = 17/59 (28%), Positives = 29/59 (49%), Gaps = 3/59 (5%) Frame = +3 Query: 348 NMGSWSYIEP---RFRTLLQNQKQIRAKSQSKGGSWLSQLFGRDESPQSNAQETETVRA 515 N W Y P R +L +++ A+ S+GG+W S G+ E + Q+ + +RA Sbjct: 56 NNNEWYYFVPLTERGGKILSVHRKVAARGGSEGGTWRSN-DGKKEIKDGHMQKGDGLRA 113 >At1g12120.1 68414.m01404 expressed protein contains Pfam domain PF05904: Plant protein of unknown function (DUF863) Length = 483 Score = 28.3 bits (60), Expect = 3.8 Identities = 12/27 (44%), Positives = 17/27 (62%) Frame = +1 Query: 325 CGARRSTRTWARGATSSRASAPSCRTR 405 C ++R +R ++S RA PSCRTR Sbjct: 259 CTTENNSRIESRRSSSPRALQPSCRTR 285 >At2g40190.1 68415.m04942 glycosyl transferase family 1 protein contains Pfam profile: PF00534 Glycosyl transferases group 1 Length = 463 Score = 27.9 bits (59), Expect = 5.1 Identities = 17/46 (36%), Positives = 23/46 (50%) Frame = -2 Query: 391 RVRKRGSM*LHEPMFLCSSWLHTS*AFGYFAISAFTWSYGNGEICS 254 RVR+R SM ++ S+WL T Y AF+W YG C+ Sbjct: 179 RVRQRNSMYNNDASIAKSNWLSTCKLVYY---RAFSWMYGMVGSCT 221 >At2g23990.2 68415.m02866 plastocyanin-like domain-containing protein Length = 226 Score = 27.9 bits (59), Expect = 5.1 Identities = 16/52 (30%), Positives = 27/52 (51%), Gaps = 4/52 (7%) Frame = -3 Query: 171 CEFAYILR----VLGGWTFFGDHSLEIGSLLHVVKGGFTFGMTQQRLGRHQD 28 C F +++ V+ G+TF + +I S+L V K + TQ+ L H+D Sbjct: 62 CSFVMMVKIRMLVIVGYTFMFKYDSKIDSVLQVTKENYEKCNTQKPLEEHKD 113 >At1g03310.2 68414.m00310 isoamylase, putative / starch debranching enzyme, putative similar to isoamylase isoform 2 [Solanum tuberosum] GI:27728147, isoamylase from [Triticum aestivum] GI:17932898, [Hordeum vulgare] GI:21314275, [Oryza sativa] GI:3252794; contains Pfam profiles PF00128: Alpha amylase catalytic domain, PF02922: Isoamylase N-terminal domain; ESTs gb|H36690, gb|AA712462, gb|AA651230 and gb|N95932 come from this gene Length = 882 Score = 27.1 bits (57), Expect = 8.9 Identities = 10/26 (38%), Positives = 14/26 (53%) Frame = -2 Query: 481 CGLSSRPNSWLNQLPPFDWDFALICF 404 CG+S+R + L PFDW+ F Sbjct: 703 CGISTRGSPLLESRKPFDWNLLASAF 728 >At1g03310.1 68414.m00309 isoamylase, putative / starch debranching enzyme, putative similar to isoamylase isoform 2 [Solanum tuberosum] GI:27728147, isoamylase from [Triticum aestivum] GI:17932898, [Hordeum vulgare] GI:21314275, [Oryza sativa] GI:3252794; contains Pfam profiles PF00128: Alpha amylase catalytic domain, PF02922: Isoamylase N-terminal domain; ESTs gb|H36690, gb|AA712462, gb|AA651230 and gb|N95932 come from this gene Length = 882 Score = 27.1 bits (57), Expect = 8.9 Identities = 10/26 (38%), Positives = 14/26 (53%) Frame = -2 Query: 481 CGLSSRPNSWLNQLPPFDWDFALICF 404 CG+S+R + L PFDW+ F Sbjct: 703 CGISTRGSPLLESRKPFDWNLLASAF 728 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,897,269 Number of Sequences: 28952 Number of extensions: 205452 Number of successful extensions: 637 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 624 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 635 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1112061928 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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