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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV10657X
         (572 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g55410.1 68416.m06154 2-oxoglutarate dehydrogenase E1 compone...    79   3e-15
At5g65750.1 68418.m08274 2-oxoglutarate dehydrogenase E1 compone...    78   4e-15
At2g16880.1 68415.m01942 pentatricopeptide (PPR) repeat-containi...    29   1.7  
At5g29070.1 68418.m03617 expressed protein                             29   2.9  
At1g03490.1 68414.m00330 no apical meristem (NAM) family protein...    29   2.9  
At1g12120.1 68414.m01404 expressed protein contains Pfam domain ...    28   3.8  
At2g40190.1 68415.m04942 glycosyl transferase family 1 protein c...    28   5.1  
At2g23990.2 68415.m02866 plastocyanin-like domain-containing pro...    28   5.1  
At1g03310.2 68414.m00310 isoamylase, putative / starch debranchi...    27   8.9  
At1g03310.1 68414.m00309 isoamylase, putative / starch debranchi...    27   8.9  

>At3g55410.1 68416.m06154 2-oxoglutarate dehydrogenase E1 component,
            putative / oxoglutarate decarboxylase, putative /
            alpha-ketoglutaric dehydrogenase, putative similar to
            SP|P20967 2-oxoglutarate dehydrogenase E1 component,
            mitochondrial precursor (EC 1.2.4.2) (Alpha-ketoglutarate
            dehydrogenase) {Saccharomyces cerevisiae}; contains Pfam
            profiles PF02779: Transketolase, pyridine binding domain,
            PF00676: Dehydrogenase E1 component
          Length = 1017

 Score = 78.6 bits (185), Expect = 3e-15
 Identities = 41/88 (46%), Positives = 59/88 (67%), Gaps = 10/88 (11%)
 Frame = +1

Query: 13   FRKPLILMTPKSLLRHPECKSS---FDDM-------KEGTNFKRVIPEEGPASENPQNVR 162
            FRKPLI+M PK+LLRH +CKS+   FDD+       K+GT FKR+I ++   S+  + +R
Sbjct: 833  FRKPLIVMAPKNLLRHKDCKSNLSEFDDVQGHPGFDKQGTRFKRLIKDQNDHSDLEEGIR 892

Query: 163  KLAFCSGRVYYDLLKQRRDKGLEKDIAI 246
            +L  CSG+VYY+L  +R+  G   D+AI
Sbjct: 893  RLVLCSGKVYYELDDERKKVG-ATDVAI 919



 Score = 69.7 bits (163), Expect = 1e-12
 Identities = 27/52 (51%), Positives = 39/52 (75%)
 Frame = +3

Query: 246  C*TEQISPFPYDQVKAEIAKYPNAQLVWSQEEHKNMGSWSYIEPRFRTLLQN 401
            C  EQ+ PFPYD ++ E+ +YPNA++VW QEE  NMG++SYI PR  T +++
Sbjct: 920  CRVEQLCPFPYDLIQRELKRYPNAEIVWCQEEAMNMGAFSYISPRLWTAMRS 971


>At5g65750.1 68418.m08274 2-oxoglutarate dehydrogenase E1 component,
            putative / oxoglutarate decarboxylase, putative /
            alpha-ketoglutaric dehydrogenase, putative similar to
            SP|P20967 2-oxoglutarate dehydrogenase E1 component,
            mitochondrial precursor (EC 1.2.4.2) (Alpha-ketoglutarate
            dehydrogenase) {Saccharomyces cerevisiae}; contains Pfam
            profiles PF02779: Transketolase, pyridine binding domain,
            PF00676: Dehydrogenase E1 component
          Length = 1025

 Score = 78.2 bits (184), Expect = 4e-15
 Identities = 41/88 (46%), Positives = 60/88 (68%), Gaps = 10/88 (11%)
 Frame = +1

Query: 13   FRKPLILMTPKSLLRHPECKSS---FDDMK-------EGTNFKRVIPEEGPASENPQNVR 162
            FRKPLI+M PK+LLRH +C S+   FDD+K       +GT FKR+I ++   S+  + +R
Sbjct: 837  FRKPLIVMAPKNLLRHKQCVSNLSEFDDVKGHPGFDKQGTRFKRLIKDQSGHSDLEEGIR 896

Query: 163  KLAFCSGRVYYDLLKQRRDKGLEKDIAI 246
            +L  CSG+VYY+L ++R+ K   KD+AI
Sbjct: 897  RLVLCSGKVYYELDEERK-KSETKDVAI 923



 Score = 65.3 bits (152), Expect = 3e-11
 Identities = 27/63 (42%), Positives = 39/63 (61%)
 Frame = +3

Query: 210  ETRQGTRKGHCYC*TEQISPFPYDQVKAEIAKYPNAQLVWSQEEHKNMGSWSYIEPRFRT 389
            E ++   K    C  EQ+ PFPYD ++ E+ +YPNA++VW QEE  NMG + YI  R  T
Sbjct: 912  ERKKSETKDVAICRVEQLCPFPYDLIQRELKRYPNAEIVWCQEEPMNMGGYQYIALRLCT 971

Query: 390  LLQ 398
             ++
Sbjct: 972  AMK 974


>At2g16880.1 68415.m01942 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 743

 Score = 29.5 bits (63), Expect = 1.7
 Identities = 20/67 (29%), Positives = 29/67 (43%), Gaps = 3/67 (4%)
 Frame = +1

Query: 46  SLLRHPECKSSFDDMKEGTNFKRVIPEEGPASENPQNVRKLAFCSGRVY---YDLLKQRR 216
           ++L +  CK    +  +  NF   + EE        N    AFC  +     YDLL +  
Sbjct: 595 NILLNGLCKEGMTE--KALNFFNTLIEEREVDTVTYNTMISAFCKDKKLKEAYDLLSEME 652

Query: 217 DKGLEKD 237
           +KGLE D
Sbjct: 653 EKGLEPD 659


>At5g29070.1 68418.m03617 expressed protein 
          Length = 307

 Score = 28.7 bits (61), Expect = 2.9
 Identities = 12/26 (46%), Positives = 17/26 (65%), Gaps = 1/26 (3%)
 Frame = +1

Query: 325 CGARRSTRTWA-RGATSSRASAPSCR 399
           CG+    R WA +GAT+   S+P+CR
Sbjct: 105 CGSDTQPRPWATQGATTGPESSPTCR 130


>At1g03490.1 68414.m00330 no apical meristem (NAM) family protein
           contains Pfam PF02365: No apical meristem (NAM) domain;
          Length = 253

 Score = 28.7 bits (61), Expect = 2.9
 Identities = 17/59 (28%), Positives = 29/59 (49%), Gaps = 3/59 (5%)
 Frame = +3

Query: 348 NMGSWSYIEP---RFRTLLQNQKQIRAKSQSKGGSWLSQLFGRDESPQSNAQETETVRA 515
           N   W Y  P   R   +L   +++ A+  S+GG+W S   G+ E    + Q+ + +RA
Sbjct: 56  NNNEWYYFVPLTERGGKILSVHRKVAARGGSEGGTWRSN-DGKKEIKDGHMQKGDGLRA 113


>At1g12120.1 68414.m01404 expressed protein contains Pfam domain
           PF05904: Plant protein of unknown function (DUF863)
          Length = 483

 Score = 28.3 bits (60), Expect = 3.8
 Identities = 12/27 (44%), Positives = 17/27 (62%)
 Frame = +1

Query: 325 CGARRSTRTWARGATSSRASAPSCRTR 405
           C    ++R  +R ++S RA  PSCRTR
Sbjct: 259 CTTENNSRIESRRSSSPRALQPSCRTR 285


>At2g40190.1 68415.m04942 glycosyl transferase family 1 protein
           contains Pfam profile: PF00534 Glycosyl transferases
           group 1
          Length = 463

 Score = 27.9 bits (59), Expect = 5.1
 Identities = 17/46 (36%), Positives = 23/46 (50%)
 Frame = -2

Query: 391 RVRKRGSM*LHEPMFLCSSWLHTS*AFGYFAISAFTWSYGNGEICS 254
           RVR+R SM  ++     S+WL T     Y    AF+W YG    C+
Sbjct: 179 RVRQRNSMYNNDASIAKSNWLSTCKLVYY---RAFSWMYGMVGSCT 221


>At2g23990.2 68415.m02866 plastocyanin-like domain-containing
           protein 
          Length = 226

 Score = 27.9 bits (59), Expect = 5.1
 Identities = 16/52 (30%), Positives = 27/52 (51%), Gaps = 4/52 (7%)
 Frame = -3

Query: 171 CEFAYILR----VLGGWTFFGDHSLEIGSLLHVVKGGFTFGMTQQRLGRHQD 28
           C F  +++    V+ G+TF   +  +I S+L V K  +    TQ+ L  H+D
Sbjct: 62  CSFVMMVKIRMLVIVGYTFMFKYDSKIDSVLQVTKENYEKCNTQKPLEEHKD 113


>At1g03310.2 68414.m00310 isoamylase, putative / starch debranching
           enzyme, putative similar to isoamylase isoform 2
           [Solanum tuberosum] GI:27728147, isoamylase from
           [Triticum aestivum] GI:17932898, [Hordeum vulgare]
           GI:21314275, [Oryza sativa] GI:3252794; contains Pfam
           profiles PF00128: Alpha amylase catalytic domain,
           PF02922: Isoamylase N-terminal domain; ESTs gb|H36690,
           gb|AA712462, gb|AA651230 and gb|N95932 come from this
           gene
          Length = 882

 Score = 27.1 bits (57), Expect = 8.9
 Identities = 10/26 (38%), Positives = 14/26 (53%)
 Frame = -2

Query: 481 CGLSSRPNSWLNQLPPFDWDFALICF 404
           CG+S+R +  L    PFDW+     F
Sbjct: 703 CGISTRGSPLLESRKPFDWNLLASAF 728


>At1g03310.1 68414.m00309 isoamylase, putative / starch debranching
           enzyme, putative similar to isoamylase isoform 2
           [Solanum tuberosum] GI:27728147, isoamylase from
           [Triticum aestivum] GI:17932898, [Hordeum vulgare]
           GI:21314275, [Oryza sativa] GI:3252794; contains Pfam
           profiles PF00128: Alpha amylase catalytic domain,
           PF02922: Isoamylase N-terminal domain; ESTs gb|H36690,
           gb|AA712462, gb|AA651230 and gb|N95932 come from this
           gene
          Length = 882

 Score = 27.1 bits (57), Expect = 8.9
 Identities = 10/26 (38%), Positives = 14/26 (53%)
 Frame = -2

Query: 481 CGLSSRPNSWLNQLPPFDWDFALICF 404
           CG+S+R +  L    PFDW+     F
Sbjct: 703 CGISTRGSPLLESRKPFDWNLLASAF 728


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,897,269
Number of Sequences: 28952
Number of extensions: 205452
Number of successful extensions: 637
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 624
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 635
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1112061928
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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