BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10618X (469 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1) (P... 65 3e-11 At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2) (P... 48 4e-06 At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2) (P... 48 4e-06 At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1) id... 47 6e-06 At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1) id... 47 6e-06 At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1) (... 38 0.003 At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative... 32 0.22 At4g30110.1 68417.m04281 ATPase E1-E2 type family protein / halo... 29 2.1 >At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1) (PRCD) identical to cDNA proteasome subunit prcd GI:2511593 Length = 233 Score = 64.9 bits (151), Expect = 3e-11 Identities = 28/70 (40%), Positives = 42/70 (60%) Frame = +1 Query: 256 KITDQIYCCRSGSGADTQAIADIVTYHLDFHKMGLGDPPLVETAAAIFRERCYNYRDSLV 435 ++TD +Y CRSGS AD+Q ++D V Y L H + G P V+ +A + R YN ++ L Sbjct: 48 QLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQHGQPATVKVSANLIRMLAYNNKNMLQ 107 Query: 436 AGILVAVWGK 465 G++V W K Sbjct: 108 TGLIVGGWDK 117 Score = 61.7 bits (143), Expect = 2e-10 Identities = 24/42 (57%), Positives = 36/42 (85%) Frame = +2 Query: 128 MNAPHSTGTSIMACEFDGGVVIGADARTTTGAYIANRVTDKL 253 ++APHS GT+I+ ++GGVV+GAD+RT+TG Y+ANR +DK+ Sbjct: 5 LDAPHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKI 46 >At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2) (PRCFC) identical to 20S proteasome beta subunit PBB2 [Arabidopsis thaliana] GI:3421104, cDNA proteasome subunit prcfc GI:2511575 Length = 274 Score = 47.6 bits (108), Expect = 4e-06 Identities = 19/60 (31%), Positives = 36/60 (60%) Frame = +1 Query: 271 IYCCRSGSGADTQAIADIVTYHLDFHKMGLGDPPLVETAAAIFRERCYNYRDSLVAGILV 450 IYCC +G+ ADT+A+ D+V+ L H+ G V TA + ++ ++Y+ + A +++ Sbjct: 80 IYCCGAGTAADTEAVTDMVSSQLRLHRYQTGRDSRVVTALTLLKKHLFSYQGHVSAALVL 139 Score = 36.7 bits (81), Expect = 0.008 Identities = 15/36 (41%), Positives = 24/36 (66%) Frame = +2 Query: 146 TGTSIMACEFDGGVVIGADARTTTGAYIANRVTDKL 253 TGT+I+ F GV++GAD R T G +A++ +K+ Sbjct: 38 TGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKI 73 >At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2) (PRCFC) identical to 20S proteasome beta subunit PBB2 [Arabidopsis thaliana] GI:3421104, cDNA proteasome subunit prcfc GI:2511575 Length = 274 Score = 47.6 bits (108), Expect = 4e-06 Identities = 19/60 (31%), Positives = 36/60 (60%) Frame = +1 Query: 271 IYCCRSGSGADTQAIADIVTYHLDFHKMGLGDPPLVETAAAIFRERCYNYRDSLVAGILV 450 IYCC +G+ ADT+A+ D+V+ L H+ G V TA + ++ ++Y+ + A +++ Sbjct: 80 IYCCGAGTAADTEAVTDMVSSQLRLHRYQTGRDSRVVTALTLLKKHLFSYQGHVSAALVL 139 Score = 36.7 bits (81), Expect = 0.008 Identities = 15/36 (41%), Positives = 24/36 (66%) Frame = +2 Query: 146 TGTSIMACEFDGGVVIGADARTTTGAYIANRVTDKL 253 TGT+I+ F GV++GAD R T G +A++ +K+ Sbjct: 38 TGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKI 73 >At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1) identical to 20S proteasome beta subunit PBB1 (PBB1) GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2), 677-692 (1998)); contains Pfam profile: PF00227 proteasome A-type and B-type; Length = 273 Score = 47.2 bits (107), Expect = 6e-06 Identities = 19/60 (31%), Positives = 36/60 (60%) Frame = +1 Query: 271 IYCCRSGSGADTQAIADIVTYHLDFHKMGLGDPPLVETAAAIFRERCYNYRDSLVAGILV 450 IYCC +G+ ADT+A+ D+V+ L H+ G V TA + ++ ++Y+ + A +++ Sbjct: 80 IYCCGAGTAADTEAVTDMVSSQLRLHRYQTGRDSRVITALTLLKKHLFSYQGHVSAALVL 139 Score = 36.7 bits (81), Expect = 0.008 Identities = 15/36 (41%), Positives = 24/36 (66%) Frame = +2 Query: 146 TGTSIMACEFDGGVVIGADARTTTGAYIANRVTDKL 253 TGT+I+ F GV++GAD R T G +A++ +K+ Sbjct: 38 TGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKI 73 >At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1) identical to 20S proteasome beta subunit PBB1 (PBB1) GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2), 677-692 (1998)); contains Pfam profile: PF00227 proteasome A-type and B-type; Length = 267 Score = 47.2 bits (107), Expect = 6e-06 Identities = 19/60 (31%), Positives = 36/60 (60%) Frame = +1 Query: 271 IYCCRSGSGADTQAIADIVTYHLDFHKMGLGDPPLVETAAAIFRERCYNYRDSLVAGILV 450 IYCC +G+ ADT+A+ D+V+ L H+ G V TA + ++ ++Y+ + A +++ Sbjct: 80 IYCCGAGTAADTEAVTDMVSSQLRLHRYQTGRDSRVITALTLLKKHLFSYQGHVSAALVL 139 Score = 36.7 bits (81), Expect = 0.008 Identities = 15/36 (41%), Positives = 24/36 (66%) Frame = +2 Query: 146 TGTSIMACEFDGGVVIGADARTTTGAYIANRVTDKL 253 TGT+I+ F GV++GAD R T G +A++ +K+ Sbjct: 38 TGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKI 73 >At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1) (PRCE) identical to GB:O23717; identical to cDNA proteasome subunit prce GI:2511595 Length = 274 Score = 38.3 bits (85), Expect = 0.003 Identities = 15/38 (39%), Positives = 26/38 (68%) Frame = +2 Query: 140 HSTGTSIMACEFDGGVVIGADARTTTGAYIANRVTDKL 253 H+ GT+ +A F GGV++ AD+R + G YI+++ K+ Sbjct: 54 HAKGTTTLAFIFKGGVMVAADSRASMGGYISSQSVKKI 91 >At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative very strong similarity to SP|O23717 Proteasome subunit beta type 5 precursor (EC 3.4.25.1) (20S proteasome subunit E) (Proteasome epsilon chain) {Arabidopsis thaliana} Length = 273 Score = 31.9 bits (69), Expect = 0.22 Identities = 13/35 (37%), Positives = 23/35 (65%) Frame = +2 Query: 149 GTSIMACEFDGGVVIGADARTTTGAYIANRVTDKL 253 GT+ +A F GV++ AD+R + G YI+++ K+ Sbjct: 57 GTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKI 91 >At4g30110.1 68417.m04281 ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein / heavy-metal-associated domain-containing protein similar to cadmium efflux pump protein from Geobacillus stearothermophilus [GI:16753175], cadmium resistance protein B from Staphylococcus aureus [GI:14020985] Length = 951 Score = 28.7 bits (61), Expect = 2.1 Identities = 11/22 (50%), Positives = 14/22 (63%) Frame = -2 Query: 357 PHLVEVQMICHNISYGLSVGTG 292 PH E+Q CH+ GL +GTG Sbjct: 837 PHQHELQQSCHDKPSGLDIGTG 858 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,615,325 Number of Sequences: 28952 Number of extensions: 224029 Number of successful extensions: 504 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 489 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 504 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 791932800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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