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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV10618X
         (469 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1) (P...    65   3e-11
At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2) (P...    48   4e-06
At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2) (P...    48   4e-06
At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1) id...    47   6e-06
At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1) id...    47   6e-06
At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1) (...    38   0.003
At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative...    32   0.22 
At4g30110.1 68417.m04281 ATPase E1-E2 type family protein / halo...    29   2.1  

>At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1)
           (PRCD) identical to cDNA proteasome subunit prcd
           GI:2511593
          Length = 233

 Score = 64.9 bits (151), Expect = 3e-11
 Identities = 28/70 (40%), Positives = 42/70 (60%)
 Frame = +1

Query: 256 KITDQIYCCRSGSGADTQAIADIVTYHLDFHKMGLGDPPLVETAAAIFRERCYNYRDSLV 435
           ++TD +Y CRSGS AD+Q ++D V Y L  H +  G P  V+ +A + R   YN ++ L 
Sbjct: 48  QLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQHGQPATVKVSANLIRMLAYNNKNMLQ 107

Query: 436 AGILVAVWGK 465
            G++V  W K
Sbjct: 108 TGLIVGGWDK 117



 Score = 61.7 bits (143), Expect = 2e-10
 Identities = 24/42 (57%), Positives = 36/42 (85%)
 Frame = +2

Query: 128 MNAPHSTGTSIMACEFDGGVVIGADARTTTGAYIANRVTDKL 253
           ++APHS GT+I+   ++GGVV+GAD+RT+TG Y+ANR +DK+
Sbjct: 5   LDAPHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKI 46


>At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2)
           (PRCFC) identical to 20S proteasome beta subunit PBB2
           [Arabidopsis thaliana] GI:3421104, cDNA proteasome
           subunit prcfc GI:2511575
          Length = 274

 Score = 47.6 bits (108), Expect = 4e-06
 Identities = 19/60 (31%), Positives = 36/60 (60%)
 Frame = +1

Query: 271 IYCCRSGSGADTQAIADIVTYHLDFHKMGLGDPPLVETAAAIFRERCYNYRDSLVAGILV 450
           IYCC +G+ ADT+A+ D+V+  L  H+   G    V TA  + ++  ++Y+  + A +++
Sbjct: 80  IYCCGAGTAADTEAVTDMVSSQLRLHRYQTGRDSRVVTALTLLKKHLFSYQGHVSAALVL 139



 Score = 36.7 bits (81), Expect = 0.008
 Identities = 15/36 (41%), Positives = 24/36 (66%)
 Frame = +2

Query: 146 TGTSIMACEFDGGVVIGADARTTTGAYIANRVTDKL 253
           TGT+I+   F  GV++GAD R T G  +A++  +K+
Sbjct: 38  TGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKI 73


>At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2)
           (PRCFC) identical to 20S proteasome beta subunit PBB2
           [Arabidopsis thaliana] GI:3421104, cDNA proteasome
           subunit prcfc GI:2511575
          Length = 274

 Score = 47.6 bits (108), Expect = 4e-06
 Identities = 19/60 (31%), Positives = 36/60 (60%)
 Frame = +1

Query: 271 IYCCRSGSGADTQAIADIVTYHLDFHKMGLGDPPLVETAAAIFRERCYNYRDSLVAGILV 450
           IYCC +G+ ADT+A+ D+V+  L  H+   G    V TA  + ++  ++Y+  + A +++
Sbjct: 80  IYCCGAGTAADTEAVTDMVSSQLRLHRYQTGRDSRVVTALTLLKKHLFSYQGHVSAALVL 139



 Score = 36.7 bits (81), Expect = 0.008
 Identities = 15/36 (41%), Positives = 24/36 (66%)
 Frame = +2

Query: 146 TGTSIMACEFDGGVVIGADARTTTGAYIANRVTDKL 253
           TGT+I+   F  GV++GAD R T G  +A++  +K+
Sbjct: 38  TGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKI 73


>At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1)
           identical to 20S proteasome beta subunit PBB1 (PBB1)
           GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
           677-692 (1998)); contains Pfam profile: PF00227
           proteasome A-type and B-type;
          Length = 273

 Score = 47.2 bits (107), Expect = 6e-06
 Identities = 19/60 (31%), Positives = 36/60 (60%)
 Frame = +1

Query: 271 IYCCRSGSGADTQAIADIVTYHLDFHKMGLGDPPLVETAAAIFRERCYNYRDSLVAGILV 450
           IYCC +G+ ADT+A+ D+V+  L  H+   G    V TA  + ++  ++Y+  + A +++
Sbjct: 80  IYCCGAGTAADTEAVTDMVSSQLRLHRYQTGRDSRVITALTLLKKHLFSYQGHVSAALVL 139



 Score = 36.7 bits (81), Expect = 0.008
 Identities = 15/36 (41%), Positives = 24/36 (66%)
 Frame = +2

Query: 146 TGTSIMACEFDGGVVIGADARTTTGAYIANRVTDKL 253
           TGT+I+   F  GV++GAD R T G  +A++  +K+
Sbjct: 38  TGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKI 73


>At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1)
           identical to 20S proteasome beta subunit PBB1 (PBB1)
           GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
           677-692 (1998)); contains Pfam profile: PF00227
           proteasome A-type and B-type;
          Length = 267

 Score = 47.2 bits (107), Expect = 6e-06
 Identities = 19/60 (31%), Positives = 36/60 (60%)
 Frame = +1

Query: 271 IYCCRSGSGADTQAIADIVTYHLDFHKMGLGDPPLVETAAAIFRERCYNYRDSLVAGILV 450
           IYCC +G+ ADT+A+ D+V+  L  H+   G    V TA  + ++  ++Y+  + A +++
Sbjct: 80  IYCCGAGTAADTEAVTDMVSSQLRLHRYQTGRDSRVITALTLLKKHLFSYQGHVSAALVL 139



 Score = 36.7 bits (81), Expect = 0.008
 Identities = 15/36 (41%), Positives = 24/36 (66%)
 Frame = +2

Query: 146 TGTSIMACEFDGGVVIGADARTTTGAYIANRVTDKL 253
           TGT+I+   F  GV++GAD R T G  +A++  +K+
Sbjct: 38  TGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKI 73


>At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1)
           (PRCE) identical to GB:O23717; identical to cDNA
           proteasome subunit prce GI:2511595
          Length = 274

 Score = 38.3 bits (85), Expect = 0.003
 Identities = 15/38 (39%), Positives = 26/38 (68%)
 Frame = +2

Query: 140 HSTGTSIMACEFDGGVVIGADARTTTGAYIANRVTDKL 253
           H+ GT+ +A  F GGV++ AD+R + G YI+++   K+
Sbjct: 54  HAKGTTTLAFIFKGGVMVAADSRASMGGYISSQSVKKI 91


>At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative
           very strong similarity to SP|O23717 Proteasome subunit
           beta type 5 precursor (EC 3.4.25.1) (20S proteasome
           subunit E) (Proteasome epsilon chain) {Arabidopsis
           thaliana}
          Length = 273

 Score = 31.9 bits (69), Expect = 0.22
 Identities = 13/35 (37%), Positives = 23/35 (65%)
 Frame = +2

Query: 149 GTSIMACEFDGGVVIGADARTTTGAYIANRVTDKL 253
           GT+ +A  F  GV++ AD+R + G YI+++   K+
Sbjct: 57  GTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKI 91


>At4g30110.1 68417.m04281 ATPase E1-E2 type family protein /
           haloacid dehalogenase-like hydrolase family protein /
           heavy-metal-associated domain-containing protein similar
           to cadmium efflux pump protein from Geobacillus
           stearothermophilus [GI:16753175], cadmium resistance
           protein B from Staphylococcus aureus [GI:14020985]
          Length = 951

 Score = 28.7 bits (61), Expect = 2.1
 Identities = 11/22 (50%), Positives = 14/22 (63%)
 Frame = -2

Query: 357 PHLVEVQMICHNISYGLSVGTG 292
           PH  E+Q  CH+   GL +GTG
Sbjct: 837 PHQHELQQSCHDKPSGLDIGTG 858


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,615,325
Number of Sequences: 28952
Number of extensions: 224029
Number of successful extensions: 504
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 489
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 504
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 791932800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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