Bombyx BLAST Result against bee

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV10576
         (666 letters)

Database: bee 
           438 sequences; 146,343 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ384991-1|ABD51779.1|   94|Apis mellifera allergen Api m 6 vari...    39   5e-05
DQ384990-1|ABD51778.1|   92|Apis mellifera allergen Api m 6 vari...    39   5e-05
AB167961-1|BAD51404.1|  554|Apis mellifera E74 protein.                25   0.86 
DQ015969-1|AAY81926.1|  397|Apis mellifera stargazin related pro...    23   2.6  

>DQ384991-1|ABD51779.1|   94|Apis mellifera allergen Api m 6 variant
           2 precursor protein.
          Length = 94

 Score = 38.7 bits (86), Expect = 5e-05
 Identities = 21/61 (34%), Positives = 27/61 (44%)
 Frame = +3

Query: 9   CSENEIYVNCVQAQCSPITCSKKDGPRICPLVEEKSCKAGCVCKEGYLKDDSGKCVAREN 188
           C  NEI+  C   +C    C       +C     K C  GCVC+ GYL++    CV R  
Sbjct: 37  CPSNEIFSRC-DGRCQRF-CPNVVPKPLCI----KICAPGCVCRLGYLRNKKKVCVPRSK 90

Query: 189 C 191
           C
Sbjct: 91  C 91



 Score = 36.7 bits (81), Expect = 2e-04
 Identities = 21/59 (35%), Positives = 26/59 (44%), Gaps = 5/59 (8%)
 Frame = +2

Query: 227 REMCPSTVQPMTCSKKDGPKICPLVEEKS-----CKAGCVCKEGYLKDDSGKCVARENC 388
           R  CPS      C  +   + CP V  K      C  GCVC+ GYL++    CV R  C
Sbjct: 34  RGKCPSNEIFSRCDGRC-QRFCPNVVPKPLCIKICAPGCVCRLGYLRNKKKVCVPRSKC 91


>DQ384990-1|ABD51778.1|   92|Apis mellifera allergen Api m 6 variant
           1 precursor protein.
          Length = 92

 Score = 38.7 bits (86), Expect = 5e-05
 Identities = 21/61 (34%), Positives = 27/61 (44%)
 Frame = +3

Query: 9   CSENEIYVNCVQAQCSPITCSKKDGPRICPLVEEKSCKAGCVCKEGYLKDDSGKCVAREN 188
           C  NEI+  C   +C    C       +C     K C  GCVC+ GYL++    CV R  
Sbjct: 37  CPSNEIFSRC-DGRCQRF-CPNVVPKPLCI----KICAPGCVCRLGYLRNKKKVCVPRSK 90

Query: 189 C 191
           C
Sbjct: 91  C 91



 Score = 36.7 bits (81), Expect = 2e-04
 Identities = 21/59 (35%), Positives = 26/59 (44%), Gaps = 5/59 (8%)
 Frame = +2

Query: 227 REMCPSTVQPMTCSKKDGPKICPLVEEKS-----CKAGCVCKEGYLKDDSGKCVARENC 388
           R  CPS      C  +   + CP V  K      C  GCVC+ GYL++    CV R  C
Sbjct: 34  RGKCPSNEIFSRCDGRC-QRFCPNVVPKPLCIKICAPGCVCRLGYLRNKKKVCVPRSKC 91


>AB167961-1|BAD51404.1|  554|Apis mellifera E74 protein.
          Length = 554

 Score = 24.6 bits (51), Expect = 0.86
 Identities = 17/58 (29%), Positives = 24/58 (41%)
 Frame = -2

Query: 464 STSLGCTVLGHSSHIFHFQSRGQNLGSFLGRHIYRYRLSDIPLYTRIRLCRTFPPPMG 291
           +TS G  +L H     H Q  G  + +  GRH+  +     P YT   +  T  P  G
Sbjct: 183 TTSTGTRLL-HGILSQHPQQHGLGVQNGYGRHLPGHAQMGRPSYTTATMATTSTPGSG 239



 Score = 22.6 bits (46), Expect = 3.5
 Identities = 12/42 (28%), Positives = 17/42 (40%)
 Frame = -1

Query: 219 HFQSRGQNLGSFLGRHIYRYRLSDIPLYTRIRLCRTFPPPMG 94
           H Q  G  + +  GRH+  +     P YT   +  T  P  G
Sbjct: 198 HPQQHGLGVQNGYGRHLPGHAQMGRPSYTTATMATTSTPGSG 239


>DQ015969-1|AAY81926.1|  397|Apis mellifera stargazin related
           protein STG-1 protein.
          Length = 397

 Score = 23.0 bits (47), Expect = 2.6
 Identities = 14/54 (25%), Positives = 23/54 (42%)
 Frame = -1

Query: 549 FQISLFTHASGFAGFSSTNGQILGPSFFEHVIGLHCAWTQFTYISFSEQRSEFG 388
           FQ   FT+  GF+     +G I       + I L+ +W Q   +    +R  +G
Sbjct: 218 FQGPPFTYRYGFSFLLYVSGFITTEVAGTYAIFLYISWHQKELVRRDSRRKNYG 271


  Database: bee
    Posted date:  Oct 23, 2007  1:17 PM
  Number of letters in database: 146,343
  Number of sequences in database:  438
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 203,454
Number of Sequences: 438
Number of extensions: 4719
Number of successful extensions: 15
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 15
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 20099475
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)