BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10574 (658 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccha... 126 2e-30 SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosacchar... 126 2e-30 SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|... 29 0.59 SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 28 1.0 SPAC30D11.08c |phf2|swp2, saf60|PHD finger containing protein Ph... 27 3.1 SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc... 26 5.5 SPCC132.01c ||SPCC1322.17c|DUF814 family protein|Schizosaccharom... 26 5.5 SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit ... 25 9.6 SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10 |Schizosac... 25 9.6 >SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 126 bits (305), Expect = 2e-30 Identities = 58/83 (69%), Positives = 68/83 (81%) Frame = +2 Query: 2 GISRDHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALRLD 181 GI+RD HKR ATG KRA RKKRK+ELGR +NTR+GP+RIH VR RGGN K+RALRLD Sbjct: 2 GITRDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRLD 61 Query: 182 TGNFSWGSECSTRKTRIIDVVYN 250 +GNFSWGSE ++KTRII V Y+ Sbjct: 62 SGNFSWGSEGVSKKTRIIQVAYH 84 Score = 77.0 bits (181), Expect = 2e-15 Identities = 42/86 (48%), Positives = 53/86 (61%) Frame = +1 Query: 256 NNELVRTKTLVKNAIVVVDATPFRQWYESHYTLPLGRKKGAKLTEAEEAIINKKRSQKTA 435 NNELVRT TL K+AIV +DA PFR WYE+HY + +G KG K T +S+ Sbjct: 87 NNELVRTNTLTKSAIVQIDAAPFRVWYETHYGILMG-SKGKKATSTP-----NPKSKHVQ 140 Query: 436 RKYLARQRLAKVEGALEEQFHTGRCW 513 RK+ AR +KV+ ALE QF GR + Sbjct: 141 RKHSARLGDSKVDSALETQFAAGRLY 166 Score = 56.8 bits (131), Expect = 3e-09 Identities = 25/38 (65%), Positives = 30/38 (78%) Frame = +3 Query: 501 GALLACVASRPGQCGRADGYILEGKELEFYLRKIKSKR 614 G L A V+SRPGQ GR DGYILEG+EL FYLR++ K+ Sbjct: 163 GRLYAVVSSRPGQSGRCDGYILEGEELHFYLRRMAPKK 200 >SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 126 bits (305), Expect = 2e-30 Identities = 58/83 (69%), Positives = 68/83 (81%) Frame = +2 Query: 2 GISRDHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALRLD 181 GI+RD HKR ATG KRA RKKRK+ELGR +NTR+GP+RIH VR RGGN K+RALRLD Sbjct: 2 GITRDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRLD 61 Query: 182 TGNFSWGSECSTRKTRIIDVVYN 250 +GNFSWGSE ++KTRII V Y+ Sbjct: 62 SGNFSWGSEGVSKKTRIIQVAYH 84 Score = 76.2 bits (179), Expect = 4e-15 Identities = 42/86 (48%), Positives = 53/86 (61%) Frame = +1 Query: 256 NNELVRTKTLVKNAIVVVDATPFRQWYESHYTLPLGRKKGAKLTEAEEAIINKKRSQKTA 435 NNELVRT TL K+AIV +DA PFR WYE+HY + +G KG K T +S+ Sbjct: 87 NNELVRTNTLTKSAIVQIDAAPFRVWYETHYGILMG-SKGKKATATP-----TPKSKHVQ 140 Query: 436 RKYLARQRLAKVEGALEEQFHTGRCW 513 RK+ AR +KV+ ALE QF GR + Sbjct: 141 RKHSARLGDSKVDSALETQFAAGRLY 166 Score = 56.8 bits (131), Expect = 3e-09 Identities = 25/38 (65%), Positives = 30/38 (78%) Frame = +3 Query: 501 GALLACVASRPGQCGRADGYILEGKELEFYLRKIKSKR 614 G L A V+SRPGQ GR DGYILEG+EL FYLR++ K+ Sbjct: 163 GRLYAVVSSRPGQSGRCDGYILEGEELHFYLRRMAPKK 200 >SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|||Manual Length = 207 Score = 29.1 bits (62), Expect = 0.59 Identities = 23/87 (26%), Positives = 42/87 (48%), Gaps = 3/87 (3%) Frame = +2 Query: 14 DHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALRLDTGNF 193 + W K T K+ +KRK L R A RL + + S+ S+GG +A + ++ Sbjct: 32 EKWSKGVKTN-KKEQEAEKRKAALERKAERERLEKEEMESLPSKGGKGSKKAAKKNSSLD 90 Query: 194 SWGSE---CSTRKTRIIDVVYNALTMN 265 ++ +E ++ R ID + L++N Sbjct: 91 AFLNETPQTASYSARNIDDALDLLSLN 117 >SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1436 Score = 28.3 bits (60), Expect = 1.0 Identities = 22/76 (28%), Positives = 33/76 (43%), Gaps = 2/76 (2%) Frame = +2 Query: 11 RDHWHKRRATGGKRAPIRKKRKY--ELGRPAANTRLGPQRIHSVRSRGGNTKYRALRLDT 184 RD +K + G K+ + EL +N + Q+ + S T YRA+ LDT Sbjct: 1083 RDRLYKSQLIGRVLDDTTKENRLLKELASSKSNITIRWQQGGLIGSGSFGTVYRAVNLDT 1142 Query: 185 GNFSWGSECSTRKTRI 232 G+ E + K RI Sbjct: 1143 GDLMAVKEVALHKPRI 1158 >SPAC30D11.08c |phf2|swp2, saf60|PHD finger containing protein Phf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 538 Score = 26.6 bits (56), Expect = 3.1 Identities = 14/32 (43%), Positives = 17/32 (53%) Frame = -2 Query: 267 QFIVNALYTTSMIRVLRVEHSDPQEKLPVSRR 172 QF + + T S +V R H DP KLP RR Sbjct: 190 QFNLEGVKTKSGRKVHRPNHFDPLVKLPTRRR 221 >SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosaccharomyces pombe|chr 2|||Manual Length = 735 Score = 25.8 bits (54), Expect = 5.5 Identities = 11/25 (44%), Positives = 15/25 (60%) Frame = -3 Query: 335 YHCLNGVASTTTIAFLTRVFVRTNS 261 ++C N V S T + + VFVR NS Sbjct: 518 FYCSNEVLSLTALLSVPNVFVRPNS 542 >SPCC132.01c ||SPCC1322.17c|DUF814 family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1021 Score = 25.8 bits (54), Expect = 5.5 Identities = 14/59 (23%), Positives = 28/59 (47%) Frame = +1 Query: 313 ATPFRQWYESHYTLPLGRKKGAKLTEAEEAIINKKRSQKTARKYLARQRLAKVEGALEE 489 +TP + H KKG+K+ A++ ++R + AR+ A + K ++E+ Sbjct: 754 STPDTTGSDIHIVSEKRGKKGSKVITAKKVSAKERREARRARRQTALEESLKAPISIED 812 >SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit Bgs2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1894 Score = 25.0 bits (52), Expect = 9.6 Identities = 11/32 (34%), Positives = 16/32 (50%) Frame = +3 Query: 105 PGSVLSVSTPFVHVVEILSTVRCVWTPVTSLG 200 P VLS P++ + L + C+W P LG Sbjct: 682 PILVLSTMKPYLCNITFLGSHLCIWQPKILLG 713 >SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 25.0 bits (52), Expect = 9.6 Identities = 8/23 (34%), Positives = 14/23 (60%) Frame = +2 Query: 14 DHWHKRRATGGKRAPIRKKRKYE 82 D W +A G +PIR+ ++Y+ Sbjct: 758 DEWKASKAFGANDSPIRENKRYK 780 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,567,887 Number of Sequences: 5004 Number of extensions: 50351 Number of successful extensions: 138 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 126 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 136 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 297805304 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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