BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10568 (489 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC17G9.07 |rps2402|rps24-2|40S ribosomal protein S24|Schizosac... 109 2e-25 SPAC17G6.06 |rps2401|rps24-1, rps24|40S ribosomal protein S24|Sc... 103 1e-23 SPCC417.06c |ppk35|mug27|serine/threonine protein kinase Ppk35|S... 28 0.87 SPAC22A12.11 |dak1||dihydroxyacetone kinase Dak1|Schizosaccharom... 27 1.1 SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 26 3.5 SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large... 25 4.6 SPAC11D3.08c |||amino acid permease, unknown 1|Schizosaccharomyc... 25 4.6 SPAC14C4.11 |||polyphosphate synthetase |Schizosaccharomyces pom... 25 6.1 SPAC1952.02 |||ribosome biogenesis protein|Schizosaccharomyces p... 25 8.1 SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr ... 25 8.1 SPBC56F2.01 |pof12||F-box protein Pof12|Schizosaccharomyces pomb... 25 8.1 SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22... 25 8.1 SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schi... 25 8.1 SPBC32H8.10 |cdk9||cyclin-dependent protein kinase Cdk9 |Schizos... 25 8.1 >SPBC17G9.07 |rps2402|rps24-2|40S ribosomal protein S24|Schizosaccharomyces pombe|chr 2|||Manual Length = 134 Score = 109 bits (262), Expect = 2e-25 Identities = 53/75 (70%), Positives = 60/75 (80%) Frame = +1 Query: 31 MSEGTATIRTRKFMTNRLLARKQMVCDVLHPGKPTVSKTEIREKLAKMYKVTPDVVFVFG 210 MSE TIRTRKFMTNRLL RKQMV D+LHPGK +SK EIREKLA+MYK + V FG Sbjct: 1 MSEAV-TIRTRKFMTNRLLQRKQMVVDILHPGKANLSKNEIREKLAQMYKTDSECVQAFG 59 Query: 211 FKTNFGGGKSTGFAL 255 +T+FGGG+STGFAL Sbjct: 60 LRTHFGGGRSTGFAL 74 Score = 50.4 bits (115), Expect = 1e-07 Identities = 26/47 (55%), Positives = 33/47 (70%), Gaps = 1/47 (2%) Frame = +3 Query: 255 IYDTLDLAKKFEPKHRLARHGLYEK-KRPTRKQRKERKNRMKKVRGT 392 IYD+ + KKFEP +RL R G E ++ R+QRK+RKNR KKV GT Sbjct: 75 IYDSTESMKKFEPHYRLVRVGQAEPIQKVARQQRKQRKNRGKKVFGT 121 >SPAC17G6.06 |rps2401|rps24-1, rps24|40S ribosomal protein S24|Schizosaccharomyces pombe|chr 1|||Manual Length = 134 Score = 103 bits (248), Expect = 1e-23 Identities = 48/75 (64%), Positives = 58/75 (77%) Frame = +1 Query: 31 MSEGTATIRTRKFMTNRLLARKQMVCDVLHPGKPTVSKTEIREKLAKMYKVTPDVVFVFG 210 MSE TIRTRKFMTNRLL RKQMV D+LHPGK +SK ++REKL +MYK V FG Sbjct: 1 MSEAV-TIRTRKFMTNRLLQRKQMVVDILHPGKANISKNDLREKLGQMYKTDASAVQAFG 59 Query: 211 FKTNFGGGKSTGFAL 255 +T++GGG++TGFAL Sbjct: 60 LRTHYGGGRTTGFAL 74 Score = 50.4 bits (115), Expect = 1e-07 Identities = 26/47 (55%), Positives = 33/47 (70%), Gaps = 1/47 (2%) Frame = +3 Query: 255 IYDTLDLAKKFEPKHRLARHGLYEK-KRPTRKQRKERKNRMKKVRGT 392 IYD ++ KKFEP +RL R G E ++ R+QRK+RKNR KKV GT Sbjct: 75 IYDDVEAMKKFEPHYRLVRVGHAEPIQKVARQQRKQRKNRGKKVFGT 121 >SPCC417.06c |ppk35|mug27|serine/threonine protein kinase Ppk35|Schizosaccharomyces pombe|chr 3|||Manual Length = 624 Score = 27.9 bits (59), Expect = 0.87 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = +3 Query: 282 KFEPKHRLARHGLYEKKRPTRKQRKERKNRMKK 380 K+ P R G + +KR RK++ E+KN K Sbjct: 539 KYRPNARKPLVGRHREKRQLRKEKPEKKNNSTK 571 >SPAC22A12.11 |dak1||dihydroxyacetone kinase Dak1|Schizosaccharomyces pombe|chr 1|||Manual Length = 580 Score = 27.5 bits (58), Expect = 1.1 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +1 Query: 148 EIREKLAKMYKVTPDVVFVFGFKTNFGG 231 +++E LAK YK+ P +F F T+ G Sbjct: 286 KVKEALAKEYKINPVRIFAGPFTTSLNG 313 >SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1828 Score = 25.8 bits (54), Expect = 3.5 Identities = 11/18 (61%), Positives = 14/18 (77%) Frame = -1 Query: 63 ASANSRCSFTHFELFSII 10 +S SRCSFT FE+ SI+ Sbjct: 777 SSVISRCSFTDFEINSIL 794 >SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large subunit Rpc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1405 Score = 25.4 bits (53), Expect = 4.6 Identities = 13/42 (30%), Positives = 22/42 (52%) Frame = -2 Query: 263 VVDKANPVDLPPPKFVLKPNTNTTSGVTLYILASFSRISVLL 138 ++D + +DLPPP ++KP T +L +R S +L Sbjct: 565 MMDASTHIDLPPPA-IIKPRCLWTGKQVFTVLMKPNRFSKVL 605 >SPAC11D3.08c |||amino acid permease, unknown 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 550 Score = 25.4 bits (53), Expect = 4.6 Identities = 12/28 (42%), Positives = 16/28 (57%), Gaps = 2/28 (7%) Frame = +1 Query: 199 FVFGFKTNFGGGKSTG--FALSTTHSIW 276 F+FG TN+ G + G F LS T +W Sbjct: 232 FIFGDFTNYSGWSNMGWAFILSFTTPVW 259 >SPAC14C4.11 |||polyphosphate synthetase |Schizosaccharomyces pombe|chr 1|||Manual Length = 734 Score = 25.0 bits (52), Expect = 6.1 Identities = 15/64 (23%), Positives = 29/64 (45%), Gaps = 1/64 (1%) Frame = +3 Query: 183 YSRCSVRIRFQDKLR-RWQVNWIRFIYDTLDLAKKFEPKHRLARHGLYEKKRPTRKQRKE 359 Y+R + +I D++R +W+ D+LD+ + RH + + P + RK Sbjct: 365 YTRTAFQIPGDDEVRINLDSDWVMIREDSLDIERPCRDPEDWHRHDIDDADFPYKHLRKG 424 Query: 360 RKNR 371 +R Sbjct: 425 EYSR 428 >SPAC1952.02 |||ribosome biogenesis protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 202 Score = 24.6 bits (51), Expect = 8.1 Identities = 9/35 (25%), Positives = 21/35 (60%) Frame = +3 Query: 276 AKKFEPKHRLARHGLYEKKRPTRKQRKERKNRMKK 380 +KK + + + +K +K++KE+K+++KK Sbjct: 125 SKKVKVRKTSGKESSKREKSKKKKEKKEKKDKLKK 159 >SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr 1|||Manual Length = 1854 Score = 24.6 bits (51), Expect = 8.1 Identities = 11/25 (44%), Positives = 16/25 (64%) Frame = -2 Query: 230 PPKFVLKPNTNTTSGVTLYILASFS 156 PPK LKP+ +T+ ++ Y LA S Sbjct: 818 PPKEPLKPSLDTSPELSKYSLAKLS 842 >SPBC56F2.01 |pof12||F-box protein Pof12|Schizosaccharomyces pombe|chr 2|||Manual Length = 440 Score = 24.6 bits (51), Expect = 8.1 Identities = 17/39 (43%), Positives = 20/39 (51%), Gaps = 5/39 (12%) Frame = +3 Query: 282 KFEPKHRLARHGLYEKKRPT-----RKQRKERKNRMKKV 383 +F KH L RHG EKKR R+Q + R KKV Sbjct: 58 EFGTKH-LRRHGRIEKKRRNWKGLFRRQSNWKDGRCKKV 95 >SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22 |Schizosaccharomyces pombe|chr 1|||Manual Length = 332 Score = 24.6 bits (51), Expect = 8.1 Identities = 10/27 (37%), Positives = 16/27 (59%) Frame = -2 Query: 158 SRISVLLTVGFPGCKTSQTICLRANNL 78 SRI + ++G K Q++CLR N + Sbjct: 48 SRIQSMASLGLERFKNLQSLCLRQNQI 74 >SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 24.6 bits (51), Expect = 8.1 Identities = 8/23 (34%), Positives = 17/23 (73%) Frame = +3 Query: 324 EKKRPTRKQRKERKNRMKKVRGT 392 E+ +P ++ +E +NR+ ++RGT Sbjct: 90 ERLKPQDERTQEERNRVDEIRGT 112 >SPBC32H8.10 |cdk9||cyclin-dependent protein kinase Cdk9 |Schizosaccharomyces pombe|chr 2|||Manual Length = 591 Score = 24.6 bits (51), Expect = 8.1 Identities = 11/39 (28%), Positives = 21/39 (53%) Frame = +3 Query: 39 RNSDYSHSQVHDQQIVGAQADGLRCFTSRKTNRQQDRDP 155 +NS++S + +Q + ADG R + + Q+ R+P Sbjct: 449 KNSEFSETNAMNQTSNHSHADGQRYYRPEQDRSQRLRNP 487 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,923,995 Number of Sequences: 5004 Number of extensions: 38713 Number of successful extensions: 134 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 118 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 132 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 190087364 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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