BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10567X (405 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC17G9.07 |rps2402|rps24-2|40S ribosomal protein S24|Schizosac... 75 6e-15 SPAC17G6.06 |rps2401|rps24-1, rps24|40S ribosomal protein S24|Sc... 71 7e-14 SPAC1952.02 |||ribosome biogenesis protein|Schizosaccharomyces p... 28 0.63 SPCC417.06c |ppk35|mug27|serine/threonine protein kinase Ppk35|S... 28 0.63 SPBP18G5.02 |||CDP-diacylglycerol-glycerol-3-phosphate3-phosphat... 26 2.6 SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schi... 25 3.4 SPAC29A4.04c |||pseudouridylate synthase |Schizosaccharomyces po... 25 3.4 SPAC11D3.08c |||amino acid permease, unknown 1|Schizosaccharomyc... 25 3.4 SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 25 4.5 SPAC14C4.11 |||polyphosphate synthetase |Schizosaccharomyces pom... 25 4.5 SPBC56F2.01 |pof12||F-box protein Pof12|Schizosaccharomyces pomb... 25 5.9 SPCC132.02 |hst2||Sir2 family histone deacetylase Hst2|Schizosac... 25 5.9 SPCC1494.09c |||sequence orphan|Schizosaccharomyces pombe|chr 3|... 25 5.9 SPAC1783.04c |hst4||Sir2 family histone deacetylase Hst4|Schizos... 25 5.9 SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces pom... 24 7.8 SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc... 24 7.8 >SPBC17G9.07 |rps2402|rps24-2|40S ribosomal protein S24|Schizosaccharomyces pombe|chr 2|||Manual Length = 134 Score = 74.5 bits (175), Expect = 6e-15 Identities = 32/59 (54%), Positives = 45/59 (76%) Frame = +3 Query: 78 QIVGAQQMVCEVLHPGKPTVSKTEIRDKLDKMYKVTPDVVFVFGFKTNFGGGKSTGFAL 254 +++ +QMV ++LHPGK +SK EIR+KL +MYK + V FG +T+FGGG+STGFAL Sbjct: 16 RLLQRKQMVVDILHPGKANLSKNEIREKLAQMYKTDSECVQAFGLRTHFGGGRSTGFAL 74 Score = 51.2 bits (117), Expect = 6e-08 Identities = 26/49 (53%), Positives = 34/49 (69%), Gaps = 1/49 (2%) Frame = +2 Query: 254 VYDTLDLAKKFEPKHRLARHGLYEK-KRPTRKQRKERKNRMKKVRGTKK 397 +YD+ + KKFEP +RL R G E ++ R+QRK+RKNR KKV GT K Sbjct: 75 IYDSTESMKKFEPHYRLVRVGQAEPIQKVARQQRKQRKNRGKKVFGTGK 123 Score = 33.1 bits (72), Expect = 0.017 Identities = 17/23 (73%), Positives = 18/23 (78%) Frame = +1 Query: 31 MSEGRATIRTRKFMTNRLLARNR 99 MSE TIRTRKFMTNRLL R + Sbjct: 1 MSEA-VTIRTRKFMTNRLLQRKQ 22 >SPAC17G6.06 |rps2401|rps24-1, rps24|40S ribosomal protein S24|Schizosaccharomyces pombe|chr 1|||Manual Length = 134 Score = 70.9 bits (166), Expect = 7e-14 Identities = 28/59 (47%), Positives = 44/59 (74%) Frame = +3 Query: 78 QIVGAQQMVCEVLHPGKPTVSKTEIRDKLDKMYKVTPDVVFVFGFKTNFGGGKSTGFAL 254 +++ +QMV ++LHPGK +SK ++R+KL +MYK V FG +T++GGG++TGFAL Sbjct: 16 RLLQRKQMVVDILHPGKANISKNDLREKLGQMYKTDASAVQAFGLRTHYGGGRTTGFAL 74 Score = 51.2 bits (117), Expect = 6e-08 Identities = 26/49 (53%), Positives = 34/49 (69%), Gaps = 1/49 (2%) Frame = +2 Query: 254 VYDTLDLAKKFEPKHRLARHGLYEK-KRPTRKQRKERKNRMKKVRGTKK 397 +YD ++ KKFEP +RL R G E ++ R+QRK+RKNR KKV GT K Sbjct: 75 IYDDVEAMKKFEPHYRLVRVGHAEPIQKVARQQRKQRKNRGKKVFGTGK 123 Score = 33.1 bits (72), Expect = 0.017 Identities = 17/23 (73%), Positives = 18/23 (78%) Frame = +1 Query: 31 MSEGRATIRTRKFMTNRLLARNR 99 MSE TIRTRKFMTNRLL R + Sbjct: 1 MSEA-VTIRTRKFMTNRLLQRKQ 22 >SPAC1952.02 |||ribosome biogenesis protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 202 Score = 27.9 bits (59), Expect = 0.63 Identities = 17/47 (36%), Positives = 25/47 (53%), Gaps = 2/47 (4%) Frame = +2 Query: 269 DLAKKFEPKHRLARHGLYEKKRPTR--KQRKERKNRMKKVRGTKKSK 403 D KK + +L ++KR R + +K K+ +KKV GTKK K Sbjct: 155 DKLKKKSKRLKLDDSHTQKRKRKVRDKESKKSSKSGLKKVSGTKKVK 201 Score = 24.6 bits (51), Expect = 5.9 Identities = 9/35 (25%), Positives = 21/35 (60%) Frame = +2 Query: 275 AKKFEPKHRLARHGLYEKKRPTRKQRKERKNRMKK 379 +KK + + + +K +K++KE+K+++KK Sbjct: 125 SKKVKVRKTSGKESSKREKSKKKKEKKEKKDKLKK 159 >SPCC417.06c |ppk35|mug27|serine/threonine protein kinase Ppk35|Schizosaccharomyces pombe|chr 3|||Manual Length = 624 Score = 27.9 bits (59), Expect = 0.63 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = +2 Query: 281 KFEPKHRLARHGLYEKKRPTRKQRKERKNRMKK 379 K+ P R G + +KR RK++ E+KN K Sbjct: 539 KYRPNARKPLVGRHREKRQLRKEKPEKKNNSTK 571 >SPBP18G5.02 |||CDP-diacylglycerol-glycerol-3-phosphate3- phosphatidyltransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 25.8 bits (54), Expect = 2.6 Identities = 16/56 (28%), Positives = 24/56 (42%) Frame = +1 Query: 127 NQPSARPRSVTSSTKCTRLLPM*CSYSVSRQTSEVASQLDSLCLRHTRSGQEVRAQ 294 N PS + +CTR P CS S+ Q + + L HT + + +R Q Sbjct: 79 NNPSLHVHILADQLRCTRESPGCCSASLLMQLKKKFPDRCEIKLYHTPNLRGLRKQ 134 >SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 25.4 bits (53), Expect = 3.4 Identities = 9/26 (34%), Positives = 18/26 (69%) Frame = +2 Query: 323 EKKRPTRKQRKERKNRMKKVRGTKKS 400 E+ +P ++ +E +NR+ ++RGT S Sbjct: 90 ERLKPQDERTQEERNRVDEIRGTPMS 115 >SPAC29A4.04c |||pseudouridylate synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 474 Score = 25.4 bits (53), Expect = 3.4 Identities = 9/26 (34%), Positives = 18/26 (69%) Frame = +2 Query: 326 KKRPTRKQRKERKNRMKKVRGTKKSK 403 +K+ +K++KE+K + +K KKS+ Sbjct: 449 RKKEAKKEKKEKKEKKEKKEKKKKSE 474 >SPAC11D3.08c |||amino acid permease, unknown 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 550 Score = 25.4 bits (53), Expect = 3.4 Identities = 12/28 (42%), Positives = 16/28 (57%), Gaps = 2/28 (7%) Frame = +3 Query: 198 FVFGFKTNFGGGKSTG--FALSTTHSIW 275 F+FG TN+ G + G F LS T +W Sbjct: 232 FIFGDFTNYSGWSNMGWAFILSFTTPVW 259 >SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1647 Score = 25.0 bits (52), Expect = 4.5 Identities = 13/29 (44%), Positives = 16/29 (55%) Frame = -2 Query: 233 ATSEVCLETEYEHYIGSNLVHFVELVTDL 147 A SE LE EYE +GS L +E T + Sbjct: 1297 AASENILEIEYEDEVGSGLGPTLEFYTSV 1325 >SPAC14C4.11 |||polyphosphate synthetase |Schizosaccharomyces pombe|chr 1|||Manual Length = 734 Score = 25.0 bits (52), Expect = 4.5 Identities = 15/64 (23%), Positives = 29/64 (45%), Gaps = 1/64 (1%) Frame = +2 Query: 182 YSRCSVRIRFQDKLR-RWQVNWIRFVYDTLDLAKKFEPKHRLARHGLYEKKRPTRKQRKE 358 Y+R + +I D++R +W+ D+LD+ + RH + + P + RK Sbjct: 365 YTRTAFQIPGDDEVRINLDSDWVMIREDSLDIERPCRDPEDWHRHDIDDADFPYKHLRKG 424 Query: 359 RKNR 370 +R Sbjct: 425 EYSR 428 >SPBC56F2.01 |pof12||F-box protein Pof12|Schizosaccharomyces pombe|chr 2|||Manual Length = 440 Score = 24.6 bits (51), Expect = 5.9 Identities = 17/39 (43%), Positives = 20/39 (51%), Gaps = 5/39 (12%) Frame = +2 Query: 281 KFEPKHRLARHGLYEKKRPT-----RKQRKERKNRMKKV 382 +F KH L RHG EKKR R+Q + R KKV Sbjct: 58 EFGTKH-LRRHGRIEKKRRNWKGLFRRQSNWKDGRCKKV 95 >SPCC132.02 |hst2||Sir2 family histone deacetylase Hst2|Schizosaccharomyces pombe|chr 3|||Manual Length = 332 Score = 24.6 bits (51), Expect = 5.9 Identities = 13/42 (30%), Positives = 21/42 (50%) Frame = +2 Query: 215 DKLRRWQVNWIRFVYDTLDLAKKFEPKHRLARHGLYEKKRPT 340 + L+R+ + + V+D K P + LA + EK RPT Sbjct: 56 NNLQRFNLPYAEAVFDLSYFRKNPRPFYELAHELMPEKYRPT 97 >SPCC1494.09c |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 157 Score = 24.6 bits (51), Expect = 5.9 Identities = 12/19 (63%), Positives = 14/19 (73%) Frame = +1 Query: 220 TSEVASQLDSLCLRHTRSG 276 T +V S LDSLCLR +SG Sbjct: 91 TFKVFSLLDSLCLRLIQSG 109 >SPAC1783.04c |hst4||Sir2 family histone deacetylase Hst4|Schizosaccharomyces pombe|chr 1|||Manual Length = 415 Score = 24.6 bits (51), Expect = 5.9 Identities = 14/64 (21%), Positives = 29/64 (45%) Frame = +1 Query: 130 QPSARPRSVTSSTKCTRLLPM*CSYSVSRQTSEVASQLDSLCLRHTRSGQEVRAQAQVSP 309 Q S+ +TSSTK T+ + + + S + + QL + E++ + + + Sbjct: 352 QKSSEQTKITSSTKITKAIGLNTKSNDSSKKDNTSFQLHQVLNSIEIPKVEIKQEVEYAT 411 Query: 310 PRPV 321 P P+ Sbjct: 412 PSPL 415 >SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 204 Score = 24.2 bits (50), Expect = 7.8 Identities = 11/34 (32%), Positives = 21/34 (61%), Gaps = 2/34 (5%) Frame = +1 Query: 55 RTRKFMTNRLLAR--NRWFAKFYIQENQPSARPR 150 +TR+ + N +A+ R+ FY+ E+ P A+P+ Sbjct: 4 QTREGLPNGWVAQWDERYKCYFYVNESDPKAKPQ 37 >SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 732 Score = 24.2 bits (50), Expect = 7.8 Identities = 10/24 (41%), Positives = 16/24 (66%) Frame = -1 Query: 234 CHLRSLS*NRIRTLHRE*PCTFCR 163 CH+ +L R+R L++ CTFC+ Sbjct: 101 CHVCAL---RLRALYKTKECTFCK 121 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,707,066 Number of Sequences: 5004 Number of extensions: 33145 Number of successful extensions: 138 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 124 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 136 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 138190552 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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