BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10562 (728 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 27 2.1 SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomy... 27 3.6 SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 26 4.8 SPAC823.12 |||zinc finger protein Pep5/Vps11 |Schizosaccharomyce... 26 4.8 SPBC1709.01 |chs2|SPBC1734.17|chitin synthase homolog Chs2|Schiz... 26 6.3 SPBC1A4.10c |pmc1|SPBP23A10.01c, med14|mediator complex subunit ... 25 8.4 >SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1150 Score = 27.5 bits (58), Expect = 2.1 Identities = 20/88 (22%), Positives = 33/88 (37%), Gaps = 3/88 (3%) Frame = +2 Query: 74 GPVSAHSVHEGVAFGHGRKLCTESVDRCSHCGGPHLREKCADYTAGIEPQC---CNCLHS 244 GP ++ +EG + H T RC C GP LR+ Y G+ C+ ++ Sbjct: 214 GPNDSYYEYEGKVYCHYH-YSTLFAARCCGCDGPILRQFVEVYRNGVSQNWHVPCHMIYK 272 Query: 245 KLRIRITTLSVSSVQSAKSGTTWQDQTR 328 +++ + K Q Q R Sbjct: 273 FWNVKLCQKTSIETSKKKDSELSQSQLR 300 >SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 435 Score = 26.6 bits (56), Expect = 3.6 Identities = 10/31 (32%), Positives = 15/31 (48%) Frame = -1 Query: 215 RFPRYNRRISRVGVGLHSDCTCPHSRCIICG 123 R P +R + R+ H +C C C +CG Sbjct: 367 RCPTCDRVVERIDGCCHMNCLCGTHFCFLCG 397 >SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1242 Score = 26.2 bits (55), Expect = 4.8 Identities = 8/19 (42%), Positives = 13/19 (68%) Frame = +3 Query: 420 NKYIWIYQHRQTNTEHVYK 476 N Y WIY H ++++H Y+ Sbjct: 548 NMYSWIYDHVFSSSDHAYE 566 >SPAC823.12 |||zinc finger protein Pep5/Vps11 |Schizosaccharomyces pombe|chr 1|||Manual Length = 906 Score = 26.2 bits (55), Expect = 4.8 Identities = 17/67 (25%), Positives = 30/67 (44%), Gaps = 1/67 (1%) Frame = +3 Query: 159 VTVEAHTYARNAPIIPRESNLSAAIVYTPSCGYGSQRFQCRVSNP-QKVGLPGKIKHGIH 335 V V H ++ N P + + I T +CG+G C NP ++ G+ H + Sbjct: 111 VLVTTHKFSINESPYPIIAVSQSPIDKTIACGFGGGLVSCYHGNPLRERGIKNSYSH--N 168 Query: 336 MTHPLSG 356 + P++G Sbjct: 169 LGEPITG 175 >SPBC1709.01 |chs2|SPBC1734.17|chitin synthase homolog Chs2|Schizosaccharomyces pombe|chr 2|||Manual Length = 926 Score = 25.8 bits (54), Expect = 6.3 Identities = 15/49 (30%), Positives = 20/49 (40%), Gaps = 2/49 (4%) Frame = -2 Query: 586 IYDFHCFFVNSXXXXXXXXXLSFLVANNFFSCFLFSHL--YTCSVFVCL 446 IY C F S ++ NN+F+ +FS L C FV L Sbjct: 684 IYFVFCVFYISLIPLHNSDNSEIVLGNNYFTTLIFSSLLILACYAFVSL 732 >SPBC1A4.10c |pmc1|SPBP23A10.01c, med14|mediator complex subunit Pmc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 879 Score = 25.4 bits (53), Expect = 8.4 Identities = 13/25 (52%), Positives = 16/25 (64%), Gaps = 3/25 (12%) Frame = +3 Query: 288 NPQKVGLPGKIKHGIHM---THPLS 353 N +K G PGKI H IH+ T P+S Sbjct: 314 NVKKEGKPGKIGHYIHIFVNTQPIS 338 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,915,413 Number of Sequences: 5004 Number of extensions: 58504 Number of successful extensions: 135 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 131 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 135 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 343230174 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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