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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV10562
         (728 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom...    27   2.1  
SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomy...    27   3.6  
SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce...    26   4.8  
SPAC823.12 |||zinc finger protein Pep5/Vps11 |Schizosaccharomyce...    26   4.8  
SPBC1709.01 |chs2|SPBC1734.17|chitin synthase homolog Chs2|Schiz...    26   6.3  
SPBC1A4.10c |pmc1|SPBP23A10.01c, med14|mediator complex subunit ...    25   8.4  

>SPBC3F6.05 |rga1||GTPase activating protein
           Rga1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1150

 Score = 27.5 bits (58), Expect = 2.1
 Identities = 20/88 (22%), Positives = 33/88 (37%), Gaps = 3/88 (3%)
 Frame = +2

Query: 74  GPVSAHSVHEGVAFGHGRKLCTESVDRCSHCGGPHLREKCADYTAGIEPQC---CNCLHS 244
           GP  ++  +EG  + H     T    RC  C GP LR+    Y  G+       C+ ++ 
Sbjct: 214 GPNDSYYEYEGKVYCHYH-YSTLFAARCCGCDGPILRQFVEVYRNGVSQNWHVPCHMIYK 272

Query: 245 KLRIRITTLSVSSVQSAKSGTTWQDQTR 328
              +++   +       K     Q Q R
Sbjct: 273 FWNVKLCQKTSIETSKKKDSELSQSQLR 300


>SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 435

 Score = 26.6 bits (56), Expect = 3.6
 Identities = 10/31 (32%), Positives = 15/31 (48%)
 Frame = -1

Query: 215 RFPRYNRRISRVGVGLHSDCTCPHSRCIICG 123
           R P  +R + R+    H +C C    C +CG
Sbjct: 367 RCPTCDRVVERIDGCCHMNCLCGTHFCFLCG 397


>SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1242

 Score = 26.2 bits (55), Expect = 4.8
 Identities = 8/19 (42%), Positives = 13/19 (68%)
 Frame = +3

Query: 420 NKYIWIYQHRQTNTEHVYK 476
           N Y WIY H  ++++H Y+
Sbjct: 548 NMYSWIYDHVFSSSDHAYE 566


>SPAC823.12 |||zinc finger protein Pep5/Vps11 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 906

 Score = 26.2 bits (55), Expect = 4.8
 Identities = 17/67 (25%), Positives = 30/67 (44%), Gaps = 1/67 (1%)
 Frame = +3

Query: 159 VTVEAHTYARNAPIIPRESNLSAAIVYTPSCGYGSQRFQCRVSNP-QKVGLPGKIKHGIH 335
           V V  H ++ N    P  +   + I  T +CG+G     C   NP ++ G+     H  +
Sbjct: 111 VLVTTHKFSINESPYPIIAVSQSPIDKTIACGFGGGLVSCYHGNPLRERGIKNSYSH--N 168

Query: 336 MTHPLSG 356
           +  P++G
Sbjct: 169 LGEPITG 175


>SPBC1709.01 |chs2|SPBC1734.17|chitin synthase homolog
           Chs2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 926

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 15/49 (30%), Positives = 20/49 (40%), Gaps = 2/49 (4%)
 Frame = -2

Query: 586 IYDFHCFFVNSXXXXXXXXXLSFLVANNFFSCFLFSHL--YTCSVFVCL 446
           IY   C F  S            ++ NN+F+  +FS L    C  FV L
Sbjct: 684 IYFVFCVFYISLIPLHNSDNSEIVLGNNYFTTLIFSSLLILACYAFVSL 732


>SPBC1A4.10c |pmc1|SPBP23A10.01c, med14|mediator complex subunit
           Pmc1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 879

 Score = 25.4 bits (53), Expect = 8.4
 Identities = 13/25 (52%), Positives = 16/25 (64%), Gaps = 3/25 (12%)
 Frame = +3

Query: 288 NPQKVGLPGKIKHGIHM---THPLS 353
           N +K G PGKI H IH+   T P+S
Sbjct: 314 NVKKEGKPGKIGHYIHIFVNTQPIS 338


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,915,413
Number of Sequences: 5004
Number of extensions: 58504
Number of successful extensions: 135
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 343230174
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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