BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10553 (655 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 167 1e-42 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 95 6e-21 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 85 6e-18 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 74 2e-14 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 72 6e-14 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 49 7e-07 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 48 1e-06 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 46 5e-06 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 41 1e-04 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 1.4 SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 27 2.4 SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces... 25 9.5 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 167 bits (406), Expect = 1e-42 Identities = 75/84 (89%), Positives = 81/84 (96%) Frame = +2 Query: 254 DLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLST 433 DLY N VMSGGTTMYPGIADRMQKEI ALAPS++K+KI+APPERKYSVWIGGSILASLST Sbjct: 292 DLYGNVVMSGGTTMYPGIADRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLST 351 Query: 434 FQQMWISKEEYDESGPGIVHRKCF 505 FQQMWISK+EYDESGPGIV+RKCF Sbjct: 352 FQQMWISKQEYDESGPGIVYRKCF 375 Score = 156 bits (378), Expect = 3e-39 Identities = 73/84 (86%), Positives = 77/84 (91%) Frame = +3 Query: 3 IVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFL 182 IVRDIKEKLCYVALDFEQE+ TAA S+SLEKSYELPDGQVITIGNERFR PEALFQPS L Sbjct: 208 IVRDIKEKLCYVALDFEQELQTAAQSSSLEKSYELPDGQVITIGNERFRAPEALFQPSAL 267 Query: 183 GMESCGIHETVYNSIMKCDVDIRK 254 G+E+ GIHE YNSIMKCDVDIRK Sbjct: 268 GLENAGIHEATYNSIMKCDVDIRK 291 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 95.5 bits (227), Expect = 6e-21 Identities = 41/83 (49%), Positives = 59/83 (71%) Frame = +3 Query: 6 VRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLG 185 VR++KEKLCYV+ D E + + +T L ++Y LPDG+VI +G+ER+ CPE LFQP +G Sbjct: 209 VREMKEKLCYVSYDLELDHKLSEETTVLMRNYTLPDGRVIKVGSERYECPECLFQPHLVG 268 Query: 186 MESCGIHETVYNSIMKCDVDIRK 254 E G+ E ++++I DVDIRK Sbjct: 269 SEQPGLSEFIFDTIQAADVDIRK 291 Score = 58.4 bits (135), Expect = 8e-10 Identities = 33/86 (38%), Positives = 53/86 (61%), Gaps = 12/86 (13%) Frame = +2 Query: 257 LYANTVMSGGTTMYPGIADRMQKEITAL--------APSTI---KIKIIAPPERKYSVWI 403 LY V+SGG++MY G+ R++KEI L P+ + K+KI P R+++V+I Sbjct: 293 LYRAIVLSGGSSMYAGLPSRLEKEIKQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFI 352 Query: 404 GGSILAS-LSTFQQMWISKEEYDESG 478 GG++LA ++ MW+SK E++E G Sbjct: 353 GGAVLADIMAQNDHMWVSKAEWEEYG 378 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 85.4 bits (202), Expect = 6e-18 Identities = 38/83 (45%), Positives = 52/83 (62%) Frame = +3 Query: 3 IVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFL 182 IVR+IKEK CYVA DF E+ + +Y+LPD Q IT+G E F PE LF P + Sbjct: 213 IVREIKEKCCYVASDFRSEIESWTEHKPQIHTYQLPDNQTITLGTECFSAPEVLFNPEMM 272 Query: 183 GMESCGIHETVYNSIMKCDVDIR 251 G E+ G+H ++ SI+ D+D+R Sbjct: 273 GSEASGLHIQLFKSILLSDIDLR 295 Score = 79.4 bits (187), Expect = 4e-16 Identities = 36/83 (43%), Positives = 58/83 (69%) Frame = +2 Query: 257 LYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTF 436 LY+N V+SGG+T+ G +R E+ A++ ++KI A PER ++ W+GGSILASLSTF Sbjct: 298 LYSNIVLSGGSTLLRGFGERFISELRAISGKKNQVKIYASPERMHNAWLGGSILASLSTF 357 Query: 437 QQMWISKEEYDESGPGIVHRKCF 505 +++ I+ EEY ++ ++ R+ F Sbjct: 358 RRLLITSEEY-KNDQNVIFRRRF 379 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 74.1 bits (174), Expect = 2e-14 Identities = 36/88 (40%), Positives = 58/88 (65%), Gaps = 6/88 (6%) Frame = +2 Query: 257 LYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPP---ERKYSVWIGGSILASL 427 L N ++ GGT++ G + R+Q E++ L P + ++KI A ER Y+ W+GGSIL+SL Sbjct: 346 LLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHASGHVVERSYASWLGGSILSSL 404 Query: 428 STFQQMWISKEEYDESGP---GIVHRKC 502 TF Q+WIS++EY+E G ++ ++C Sbjct: 405 GTFHQLWISRQEYEEHGSDRLALIEKRC 432 Score = 34.3 bits (75), Expect(2) = 4e-04 Identities = 14/35 (40%), Positives = 21/35 (60%) Frame = +3 Query: 69 AAASTSLEKSYELPDGQVITIGNERFRCPEALFQP 173 ++ +T E +E PDG + G ER++ PE LF P Sbjct: 254 SSETTIAESEFEFPDGSRMMFGAERYQIPEHLFVP 288 Score = 24.2 bits (50), Expect(2) = 4e-04 Identities = 10/18 (55%), Positives = 12/18 (66%) Frame = +3 Query: 198 GIHETVYNSIMKCDVDIR 251 GI + N I +CDVDIR Sbjct: 326 GIPQLFQNCISECDVDIR 343 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 72.1 bits (169), Expect = 6e-14 Identities = 35/76 (46%), Positives = 49/76 (64%), Gaps = 2/76 (2%) Frame = +2 Query: 257 LYANTVMSGGTTMYPGIADRMQKEITALAP-STIKIKIIAPPER-KYSVWIGGSILASLS 430 L N V++GGT++ PG+++R+Q E+ LA S I + +VW GGSILASL Sbjct: 343 LLNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSILASLD 402 Query: 431 TFQQMWISKEEYDESG 478 FQ +W+SK+EYDE G Sbjct: 403 NFQHLWVSKQEYDEVG 418 Score = 54.0 bits (124), Expect = 2e-08 Identities = 30/70 (42%), Positives = 36/70 (51%), Gaps = 9/70 (12%) Frame = +3 Query: 69 AAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESC---------GIHETVYN 221 A AS+ K +E PDG G ERFR E LF PSF S G+HE VY Sbjct: 271 AKASSRNPKPFEFPDGVTHKFGQERFRISEILFNPSFSASRSAETTPPQGSVGLHELVYQ 330 Query: 222 SIMKCDVDIR 251 SI+ CD ++R Sbjct: 331 SILACDSELR 340 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 48.8 bits (111), Expect = 7e-07 Identities = 33/95 (34%), Positives = 46/95 (48%), Gaps = 20/95 (21%) Frame = +3 Query: 3 IVRDIKEKLCYVALDFEQEMATAAAS--TSLEKSYELPD------------------GQV 122 IV +IKE +CYV+ +F+++M + LE Y LPD QV Sbjct: 214 IVNEIKESVCYVSQNFKEDMEICHEKPRSKLEICYALPDYSTGKHGYIVRDINQKIEQQV 273 Query: 123 ITIGNERFRCPEALFQPSFLGMESCGIHETVYNSI 227 + + NERF PE LF PS + + GI E V S+ Sbjct: 274 LNLSNERFMIPELLFSPSDIEIREAGIPEAVMQSV 308 Score = 45.2 bits (102), Expect = 8e-06 Identities = 25/81 (30%), Positives = 39/81 (48%) Frame = +2 Query: 257 LYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTF 436 L N V GG +PG R+ E+ +LAP+ ++K+ P + W S + L + Sbjct: 319 LLENVVTIGGNCKFPGFHKRLSSELRSLAPANWEVKVFEPSDPICFPWKKASHM-PLEHW 377 Query: 437 QQMWISKEEYDESGPGIVHRK 499 I++ EY E G I+ RK Sbjct: 378 NANKITRSEYSEHGANIMTRK 398 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 48.0 bits (109), Expect = 1e-06 Identities = 26/94 (27%), Positives = 46/94 (48%), Gaps = 14/94 (14%) Frame = +2 Query: 257 LYANTVMSGGTTMYPGIADRMQKEITALAPSTIK--------------IKIIAPPERKYS 394 LY N V+SGG+T++ +R+Q+++ + I + +I+ ++ + Sbjct: 326 LYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERIHRSEMLSGAKSGGVDVNVISHKRQRNA 385 Query: 395 VWIGGSILASLSTFQQMWISKEEYDESGPGIVHR 496 VW GGS+LA F +K +Y+E G I R Sbjct: 386 VWFGGSLLAQTPEFGSYCHTKADYEEYGASIARR 419 Score = 35.9 bits (79), Expect = 0.005 Identities = 27/85 (31%), Positives = 37/85 (43%), Gaps = 5/85 (5%) Frame = +3 Query: 15 IKEKLCYVALDFEQEMATAAASTS--LEKSYELPDGQVITI--GNERFRCPEALFQPSFL 182 IKE+ CYV D +E + L+ + E G TI G ERF PE F P Sbjct: 240 IKEECCYVCPDIVKEFSRFDREPDRYLKYASESITGHSTTIDVGFERFLAPEIFFNPEIA 299 Query: 183 GME-SCGIHETVYNSIMKCDVDIRK 254 + + E V N + +D+RK Sbjct: 300 SSDFLTPLPELVDNVVQSSPIDVRK 324 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 46.0 bits (104), Expect = 5e-06 Identities = 21/75 (28%), Positives = 38/75 (50%) Frame = +2 Query: 257 LYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTF 436 L +N +++GG PG+ R+++E+T++ P I + W G S + F Sbjct: 634 LVSNVLITGGLGSLPGMETRIKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKF 693 Query: 437 QQMWISKEEYDESGP 481 + +++EEY E GP Sbjct: 694 KAAKVTREEYLEKGP 708 Score = 30.3 bits (65), Expect = 0.25 Identities = 13/32 (40%), Positives = 21/32 (65%) Frame = +3 Query: 138 ERFRCPEALFQPSFLGMESCGIHETVYNSIMK 233 E+ R PE +F PS +G++ GI E + SI++ Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILE-IMRSILQ 624 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 41.1 bits (92), Expect = 1e-04 Identities = 19/85 (22%), Positives = 41/85 (48%), Gaps = 3/85 (3%) Frame = +2 Query: 254 DLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPP---ERKYSVWIGGSILAS 424 +LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I Sbjct: 528 NLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYNR 587 Query: 425 LSTFQQMWISKEEYDESGPGIVHRK 499 + ++WI ++ G ++ K Sbjct: 588 IRIVSELWIKNSDWKMLGSRVLQYK 612 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 27.9 bits (59), Expect = 1.4 Identities = 13/37 (35%), Positives = 23/37 (62%), Gaps = 3/37 (8%) Frame = +2 Query: 398 WIGGSILASLSTFQQM---WISKEEYDESGPGIVHRK 499 ++GGSI+A S + + +++ EEY + GP +H K Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522 >SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 27.1 bits (57), Expect = 2.4 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = +2 Query: 323 KEITALAPSTIKIKIIAPPERKYSVWIGGSILASLST 433 KEI++ A S I + + P E+ W ILA L+T Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248 >SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces pombe|chr 3|||Manual Length = 879 Score = 25.0 bits (52), Expect = 9.5 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = +1 Query: 349 DHQDQDHRSPREEVLRMDRWIHPG 420 D D+ R+ E VLRM R++ PG Sbjct: 499 DIDDKKDRALSEHVLRMHRYLPPG 522 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,289,877 Number of Sequences: 5004 Number of extensions: 42724 Number of successful extensions: 135 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 114 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 128 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 295793106 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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