BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10525 (737 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC694.03 |||conserved fungal protein|Schizosaccharomyces pombe... 27 2.8 SPAC20G4.04c |hus1||checkpoint clamp complex protein Hus1|Schizo... 27 2.8 SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces... 25 8.5 >SPAC694.03 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 249 Score = 27.1 bits (57), Expect = 2.8 Identities = 9/40 (22%), Positives = 24/40 (60%) Frame = +1 Query: 601 KLFMIE*NNVLRLCRTHYYLQKVSRQHMSNYYSCAAISSF 720 +++++ + ++R+ YY +K +Q + ++SC+ I F Sbjct: 124 QVYLVGFDTLIRILDCKYYKEKAMQQVLQPFFSCSQILCF 163 >SPAC20G4.04c |hus1||checkpoint clamp complex protein Hus1|Schizosaccharomyces pombe|chr 1|||Manual Length = 287 Score = 27.1 bits (57), Expect = 2.8 Identities = 13/24 (54%), Positives = 18/24 (75%) Frame = +3 Query: 90 IEVNEIPSRYVVCSSADSVINIKV 161 +EV I YVV S+AD+VIN++V Sbjct: 53 LEVETIFEDYVVQSNADNVINLEV 76 >SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 995 Score = 25.4 bits (53), Expect = 8.5 Identities = 8/25 (32%), Positives = 18/25 (72%) Frame = +2 Query: 617 NKIMYYDFVEHIIIYKKCRDSICLT 691 N ++YD+ +H +IY++ ++I L+ Sbjct: 823 NSTIWYDWYDHKVIYRQHNENITLS 847 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,910,782 Number of Sequences: 5004 Number of extensions: 60691 Number of successful extensions: 129 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 122 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 129 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 349251756 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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