BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10522 (645 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 97 1e-21 SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 97 2e-21 SPAC8F11.06 |||nuclear envelope protein |Schizosaccharomyces pom... 28 1.0 SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S... 27 2.3 SPBC36.04 |cys11|cys1a|cysteine synthase |Schizosaccharomyces po... 25 9.3 SPCC14G10.04 |||sequence orphan|Schizosaccharomyces pombe|chr 3|... 25 9.3 SPCC1672.10 |mis16||kinetochore protein Mis16 |Schizosaccharomyc... 25 9.3 SPAC8E11.01c ||SPAC959.01|beta-fructofuranosidase|Schizosaccharo... 25 9.3 SPAC6F6.07c |rps13||40S ribosomal protein S13|Schizosaccharomyce... 25 9.3 >SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 97.5 bits (232), Expect = 1e-21 Identities = 44/83 (53%), Positives = 57/83 (68%) Frame = +1 Query: 250 GTLIIFNKDQGLTRAFRNIPGVEXXXXXXXXXXXXAPGGHLGRFVIWTQSAFGRLDPLFG 429 G L++FN+D G+ +AFRNIPGVE APGGHLGRFVIWT+SAFG LD +FG Sbjct: 206 GPLVVFNEDAGIVKAFRNIPGVEIVNVRRLNLLQLAPGGHLGRFVIWTKSAFGLLDSVFG 265 Query: 430 SWKTPSKQKKNFNLPQPKMANTD 498 S ++ KKN+ LP+ ++N D Sbjct: 266 STTEAAQLKKNYFLPENIISNAD 288 Score = 60.5 bits (140), Expect = 2e-10 Identities = 25/46 (54%), Positives = 37/46 (80%) Frame = +2 Query: 119 QQDQXAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRKGP 256 Q+ + AV L+ +KA+ D++KV S++LRAGKGK+RNRR +QR+GP Sbjct: 162 QKTKEAVALLKEIKAYRDVVKVANSRKLRAGKGKLRNRRHVQRRGP 207 Score = 39.5 bits (88), Expect = 4e-04 Identities = 18/26 (69%), Positives = 20/26 (76%) Frame = +3 Query: 51 ARGHIIEKIPELPLVVADKVQEINKT 128 ARGH IE+IPE+PLVV D VQ KT Sbjct: 139 ARGHRIEEIPEVPLVVDDAVQSFQKT 164 Score = 31.9 bits (69), Expect = 0.081 Identities = 17/40 (42%), Positives = 25/40 (62%), Gaps = 2/40 (5%) Frame = +3 Query: 510 LKSDEIRKVLRAPN-KRVIRA-TRKLNPLTNNKAMLKLNP 623 + SDEI+ +++A RV RA +K NPL N + +LNP Sbjct: 293 INSDEIQSIVKAAGPSRVKRAHVQKKNPLKNKAVLARLNP 332 >SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 97.1 bits (231), Expect = 2e-21 Identities = 44/83 (53%), Positives = 57/83 (68%) Frame = +1 Query: 250 GTLIIFNKDQGLTRAFRNIPGVEXXXXXXXXXXXXAPGGHLGRFVIWTQSAFGRLDPLFG 429 G L++FN+D G+ +AFRNIPGVE APGGHLGRFVIWT+SAFG LD +FG Sbjct: 206 GPLVVFNEDTGIVKAFRNIPGVEIVNVRRLNLLQLAPGGHLGRFVIWTKSAFGLLDSVFG 265 Query: 430 SWKTPSKQKKNFNLPQPKMANTD 498 S ++ KKN+ LP+ ++N D Sbjct: 266 STTEVAQLKKNYFLPENIISNAD 288 Score = 60.9 bits (141), Expect = 2e-10 Identities = 25/46 (54%), Positives = 37/46 (80%) Frame = +2 Query: 119 QQDQXAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRKGP 256 Q+ + AV L+ +KA+ D++KV S++LRAGKGK+RNRR +QR+GP Sbjct: 162 QKTKEAVALLKEIKAYRDVIKVANSRKLRAGKGKLRNRRHVQRRGP 207 Score = 39.5 bits (88), Expect = 4e-04 Identities = 18/26 (69%), Positives = 20/26 (76%) Frame = +3 Query: 51 ARGHIIEKIPELPLVVADKVQEINKT 128 ARGH IE+IPE+PLVV D VQ KT Sbjct: 139 ARGHRIEEIPEVPLVVDDAVQSFQKT 164 Score = 31.5 bits (68), Expect = 0.11 Identities = 17/40 (42%), Positives = 25/40 (62%), Gaps = 2/40 (5%) Frame = +3 Query: 510 LKSDEIRKVLRAPN-KRVIRA-TRKLNPLTNNKAMLKLNP 623 + SDEI+ +++A RV RA +K NPL N + +LNP Sbjct: 293 INSDEIQSIVKAAGPSRVKRAHVQKKNPLKNKAVLSRLNP 332 >SPAC8F11.06 |||nuclear envelope protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 297 Score = 28.3 bits (60), Expect = 1.0 Identities = 24/83 (28%), Positives = 37/83 (44%), Gaps = 6/83 (7%) Frame = +3 Query: 390 DSVRIRQA*PLIRVMEDTIKTKEELQPAPTEDGQH*PHTSLKSDEIRKVLR--APN---K 554 DSV P +++ KT+ E P P + +LK +R+ LR PN Sbjct: 16 DSVLNTPIRPKYEILKSKKKTQNENDPEPMDISMSPDEKNLKKSTVRRKLRKSKPNSSSN 75 Query: 555 RVIRATRKLNPLTN-NKAMLKLN 620 +V TR L +N + A++K N Sbjct: 76 QVSSRTRALTKRSNSSNAIIKAN 98 >SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 971 Score = 27.1 bits (57), Expect = 2.3 Identities = 15/53 (28%), Positives = 21/53 (39%) Frame = -2 Query: 569 STDHAFVGSTEDLPDLIRLERRVRSVLAIFGWGRLKFFFCFDGVFHDPNKGSS 411 S H +V + E +P L R +L GW + G +PNK S Sbjct: 600 SLAHVWVVAYEFVPGGPILRERTSYILIFIGWNLAALVPAYSGESKEPNKADS 652 >SPBC36.04 |cys11|cys1a|cysteine synthase |Schizosaccharomyces pombe|chr 2|||Manual Length = 351 Score = 25.0 bits (52), Expect = 9.3 Identities = 16/60 (26%), Positives = 25/60 (41%), Gaps = 3/60 (5%) Frame = +2 Query: 389 GLSPHSAGLTPYSGH-GRHHQNKRRTSTCPNRRWPTLTSHVSQV**DQE--GPPCSQQTR 559 G H + P+S +HQ +R + PN W +V+ + E GP QT+ Sbjct: 120 GAEVHPVPVAPFSNPLNYNHQARRHAESTPNASWTDQFDNVANLLSHYETTGPEIWDQTK 179 >SPCC14G10.04 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 497 Score = 25.0 bits (52), Expect = 9.3 Identities = 11/26 (42%), Positives = 14/26 (53%) Frame = +2 Query: 377 VSSSGLSPHSAGLTPYSGHGRHHQNK 454 VS S HS P+S GRH++ K Sbjct: 120 VSRSSSIGHSGSTAPWSSVGRHNRKK 145 >SPCC1672.10 |mis16||kinetochore protein Mis16 |Schizosaccharomyces pombe|chr 3|||Manual Length = 430 Score = 25.0 bits (52), Expect = 9.3 Identities = 9/19 (47%), Positives = 9/19 (47%) Frame = +2 Query: 431 HGRHHQNKRRTSTCPNRRW 487 HG H S CPN RW Sbjct: 378 HGGHTNRISEFSWCPNERW 396 >SPAC8E11.01c ||SPAC959.01|beta-fructofuranosidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 508 Score = 25.0 bits (52), Expect = 9.3 Identities = 10/37 (27%), Positives = 19/37 (51%) Frame = -2 Query: 623 RIEFQHRLVISEWVQFACSTDHAFVGSTEDLPDLIRL 513 ++ F H L + +WV+ + +G + PD +RL Sbjct: 173 KVLFYHSLNLRDWVKLSEFGSAGVLGYQYECPDFVRL 209 >SPAC6F6.07c |rps13||40S ribosomal protein S13|Schizosaccharomyces pombe|chr 1|||Manual Length = 151 Score = 25.0 bits (52), Expect = 9.3 Identities = 11/25 (44%), Positives = 13/25 (52%) Frame = +2 Query: 362 EAILDVSSSGLSPHSAGLTPYSGHG 436 E IL S G+SP G+T HG Sbjct: 35 EQILKFSKKGMSPSQIGVTLRDSHG 59 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,451,384 Number of Sequences: 5004 Number of extensions: 46379 Number of successful extensions: 116 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 108 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 116 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 289756512 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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