BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10522 (645 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_19071| Best HMM Match : Ribosomal_L4 (HMM E-Value=0) 53 2e-07 SB_14316| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.2 SB_31780| Best HMM Match : Pkinase (HMM E-Value=0) 28 7.5 SB_22853| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 9.9 >SB_19071| Best HMM Match : Ribosomal_L4 (HMM E-Value=0) Length = 299 Score = 53.2 bits (122), Expect = 2e-07 Identities = 24/43 (55%), Positives = 27/43 (62%) Frame = +1 Query: 250 GTLIIFNKDQGLTRAFRNIPGVEXXXXXXXXXXXXAPGGHLGR 378 G LII+N DQGL +AFRN+PGVE PGGHLGR Sbjct: 203 GPLIIYNNDQGLRQAFRNLPGVELQHVDRLNLLKLCPGGHLGR 245 Score = 50.8 bits (116), Expect = 9e-07 Identities = 22/41 (53%), Positives = 30/41 (73%) Frame = +2 Query: 134 AVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRKGP 256 AV L+ + A+ D+ K S+++RAGKGKMRNRR + RKGP Sbjct: 164 AVKLLKAVNAYEDVEKCIDSKKIRAGKGKMRNRRTVMRKGP 204 Score = 38.3 bits (85), Expect = 0.005 Identities = 15/26 (57%), Positives = 21/26 (80%) Frame = +3 Query: 51 ARGHIIEKIPELPLVVADKVQEINKT 128 ARGH IEKI E+PLV++D ++ + KT Sbjct: 136 ARGHRIEKIAEVPLVISDAIESVTKT 161 >SB_14316| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1472 Score = 29.1 bits (62), Expect = 3.2 Identities = 16/38 (42%), Positives = 23/38 (60%), Gaps = 3/38 (7%) Frame = +3 Query: 513 KSDEIRKVLRAPNKRV---IRATRKLNPLTNNKAMLKL 617 +SD I K+ NK++ ++ L+ LTNNKA LKL Sbjct: 27 QSDVIHKIPNEANKQIGLRVKCLALLDYLTNNKAQLKL 64 >SB_31780| Best HMM Match : Pkinase (HMM E-Value=0) Length = 964 Score = 27.9 bits (59), Expect = 7.5 Identities = 13/45 (28%), Positives = 26/45 (57%) Frame = -1 Query: 396 LSPDDETSKMASRSQLQEVQLVNIQELHTGDVAEGASQTLILVED 262 L+ D+E + + L++V + + + T VA G S TL+L+++ Sbjct: 557 LALDNEDGPVEEVAVLRQVSVPDFNSVGTSSVAMGTSHTLVLLQN 601 >SB_22853| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 134 Score = 27.5 bits (58), Expect = 9.9 Identities = 14/30 (46%), Positives = 19/30 (63%), Gaps = 1/30 (3%) Frame = -1 Query: 384 DETSKMASRSQLQEVQLVNIQE-LHTGDVA 298 DETS +SRS++Q LV E LH D++ Sbjct: 39 DETSSPSSRSRIQASSLVKTNEFLHARDIS 68 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 19,519,665 Number of Sequences: 59808 Number of extensions: 388079 Number of successful extensions: 1129 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 1045 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1128 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1633044375 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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