BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10521 (744 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein 5|... 27 2.8 SPAC3H8.02 |||sec14 cytosolic factor family|Schizosaccharomyces ... 26 6.5 SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 26 6.5 SPBC3F6.02c |||3 beta-hydroxysteroid dehydrogenase/delta 5-->4-i... 25 8.6 >SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein 5|Schizosaccharomyces pombe|chr 3|||Manual Length = 736 Score = 27.1 bits (57), Expect = 2.8 Identities = 12/31 (38%), Positives = 21/31 (67%) Frame = +1 Query: 31 EESIAKAVRYSNVVINLVGRDYETKNFKYND 123 +ESI+ + SN+ ++L R Y+TK K+N+ Sbjct: 166 KESISSSTN-SNIKLHLAARKYQTKPEKFNE 195 >SPAC3H8.02 |||sec14 cytosolic factor family|Schizosaccharomyces pombe|chr 1|||Manual Length = 444 Score = 25.8 bits (54), Expect = 6.5 Identities = 21/74 (28%), Positives = 35/74 (47%) Frame = +1 Query: 19 HLLDEESIAKAVRYSNVVINLVGRDYETKNFKYNDVHVDGVRRIARICREEGVERFIHLS 198 H L S AK+ + + G D + N K +VH++ V +IA EG+ R Sbjct: 58 HRLSSVSYAKSRTRLELTSSSHGSDTRSFNDKTKNVHLERVEKIASEWDPEGL-RVCFWD 116 Query: 199 YLNAEEHPKPLVLK 240 +N ++ P L+L+ Sbjct: 117 AVNCDD-PDGLLLR 129 >SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1610 Score = 25.8 bits (54), Expect = 6.5 Identities = 10/22 (45%), Positives = 16/22 (72%) Frame = +3 Query: 402 LYKNGLATVKQPVFVSDVAQGI 467 L K GL++ K P+FVS + +G+ Sbjct: 1016 LGKLGLSSSKSPIFVSSILEGL 1037 >SPBC3F6.02c |||3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 340 Score = 25.4 bits (53), Expect = 8.6 Identities = 12/47 (25%), Positives = 23/47 (48%) Frame = +1 Query: 58 YSNVVINLVGRDYETKNFKYNDVHVDGVRRIARICREEGVERFIHLS 198 + VVI+ + Y +V+VDG I + C++ V+ ++ S Sbjct: 70 HPRVVIHTASPVHNLARDIYFEVNVDGTANIIKACQKFNVDALVYTS 116 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,020,774 Number of Sequences: 5004 Number of extensions: 61614 Number of successful extensions: 164 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 161 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 164 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 353266144 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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