BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10503 (838 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 157 1e-39 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 75 1e-14 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 69 6e-13 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 42 8e-05 SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizos... 28 1.4 SPCC1450.12 |||conserved fungal protein|Schizosaccharomyces pomb... 28 1.4 SPBC12D12.05c |||mitochondrial carrier, calcium binding subfamil... 27 3.3 SPAC22E12.04 |ccs1|pccs, pccs|metallochaperone Ccs1 |Schizosacch... 27 4.4 SPAC4F10.02 |||aminopeptidase |Schizosaccharomyces pombe|chr 1||... 26 5.8 SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom... 26 5.8 SPBC31E1.04 |pep12||SNARE Pep12|Schizosaccharomyces pombe|chr 2|... 26 5.8 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 157 bits (382), Expect = 1e-39 Identities = 68/84 (80%), Positives = 79/84 (94%) Frame = +1 Query: 1 KNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFL 180 KNS+YFVEWIP+NV AVC +PP+ LKM+ATFIGNST+IQE+F+R+ +QF+AMFRRKAFL Sbjct: 336 KNSAYFVEWIPDNVLKAVCSVPPKDLKMSATFIGNSTSIQEIFRRLGDQFSAMFRRKAFL 395 Query: 181 HWYTGEGMDEMEFTEAESNMNDLV 252 HWYTGEGMDEMEFTEAESNMNDLV Sbjct: 396 HWYTGEGMDEMEFTEAESNMNDLV 419 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 74.9 bits (176), Expect = 1e-14 Identities = 32/88 (36%), Positives = 52/88 (59%), Gaps = 8/88 (9%) Frame = +1 Query: 1 KNSSYFVEWIPNNVKTAVCDIPPRGLK--------MAATFIGNSTAIQELFKRISEQFTA 156 K + FV+W P K +CD PP+ ++ A + N+T+I E + R+ +F Sbjct: 338 KRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDL 397 Query: 157 MFRRKAFLHWYTGEGMDEMEFTEAESNM 240 M+ ++AF+HWY GEGM+E EF+EA ++ Sbjct: 398 MYSKRAFVHWYVGEGMEEGEFSEAREDL 425 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 69.3 bits (162), Expect = 6e-13 Identities = 30/83 (36%), Positives = 48/83 (57%), Gaps = 8/83 (9%) Frame = +1 Query: 16 FVEWIPNNVKTAVCDIPPRGL--------KMAATFIGNSTAIQELFKRISEQFTAMFRRK 171 FV+W P K +C PP+ + A + N+T+I E + R+ +F M+ ++ Sbjct: 347 FVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVCMLSNTTSIAEAWSRLDHKFDLMYSKR 406 Query: 172 AFLHWYTGEGMDEMEFTEAESNM 240 AF+HWY GEGM+E EF+EA ++ Sbjct: 407 AFVHWYVGEGMEEGEFSEAREDL 429 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 42.3 bits (95), Expect = 8e-05 Identities = 22/77 (28%), Positives = 40/77 (51%), Gaps = 3/77 (3%) Frame = +1 Query: 16 FVEWIPNNVKTAVCDIPP---RGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHW 186 F+ W P +++ A+ P +++ + N T+I LFKR +Q+ + +R AFL Sbjct: 348 FIPWGPASIQVALSKKSPYIKTNHRVSGLMLANHTSIASLFKRTLDQYDRLRKRNAFLEQ 407 Query: 187 YTGEGMDEMEFTEAESN 237 Y E + E + E +S+ Sbjct: 408 YKKEAIFEDDLNEFDSS 424 >SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizosaccharomyces pombe|chr 2|||Manual Length = 872 Score = 28.3 bits (60), Expect = 1.4 Identities = 14/36 (38%), Positives = 21/36 (58%) Frame = +1 Query: 103 NSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDE 210 NS+ IQ L K I+ T +R ++ Y+G G+DE Sbjct: 193 NSSEIQALEKSINTFTTYQYRAPEMINLYSGLGIDE 228 >SPCC1450.12 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 821 Score = 28.3 bits (60), Expect = 1.4 Identities = 12/37 (32%), Positives = 20/37 (54%) Frame = +3 Query: 495 RYVAVQVVFTFPVFFLDYEGQLWNVYCSKQLPSTTRA 605 R++ + + TFP D + QLWNV L S +++ Sbjct: 65 RFIFKRFILTFPFLDPDSQNQLWNVNFRNLLKSLSKS 101 >SPBC12D12.05c |||mitochondrial carrier, calcium binding subfamily|Schizosaccharomyces pombe|chr 2|||Manual Length = 426 Score = 27.1 bits (57), Expect = 3.3 Identities = 9/20 (45%), Positives = 11/20 (55%) Frame = +3 Query: 60 HSPSWSQDGRHLHWKLHRHP 119 H + RH +WKLH HP Sbjct: 65 HKQKFEHQFRHGYWKLHPHP 84 >SPAC22E12.04 |ccs1|pccs, pccs|metallochaperone Ccs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 297 Score = 26.6 bits (56), Expect = 4.4 Identities = 11/28 (39%), Positives = 16/28 (57%) Frame = -1 Query: 250 QGRSCCSQPQ*TPSRPCPRRCTNARKPC 167 +G SCCS+ +PS P C+ +K C Sbjct: 221 EGSSCCSKKDSSPSEK-PSCCSQEKKSC 247 >SPAC4F10.02 |||aminopeptidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 467 Score = 26.2 bits (55), Expect = 5.8 Identities = 9/23 (39%), Positives = 14/23 (60%) Frame = -2 Query: 444 SYYINTKSKSIVDLSKGERWKGG 376 SY++ SI+ S G++WK G Sbjct: 56 SYFVTRNKSSIIAFSIGKKWKPG 78 >SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharomyces pombe|chr 2|||Manual Length = 1462 Score = 26.2 bits (55), Expect = 5.8 Identities = 16/53 (30%), Positives = 25/53 (47%), Gaps = 3/53 (5%) Frame = -2 Query: 201 ALAGVPMQESLAPEHSGEL---FRDALEQLLDGGGVSNEGGGHLETTRGNVAH 52 A G+P +S+ HS EL F L+ ++ SNE H + TR + + Sbjct: 1212 ASKGLPFDKSILDNHSAELNDAFNLFLKAAVEYKVDSNESSEHFQDTRWKIIY 1264 >SPBC31E1.04 |pep12||SNARE Pep12|Schizosaccharomyces pombe|chr 2|||Manual Length = 317 Score = 26.2 bits (55), Expect = 5.8 Identities = 13/27 (48%), Positives = 17/27 (62%) Frame = +3 Query: 417 FCFSYLYSNFNSFRLQHA**NNLGSTR 497 FCF ++ F+SFR Q+A NL S R Sbjct: 243 FCFLKSFAMFSSFRSQNANLYNLNSIR 269 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,024,355 Number of Sequences: 5004 Number of extensions: 54830 Number of successful extensions: 154 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 150 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 152 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 412451140 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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