Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV10477
(820 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 158 8e-40
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 157 2e-39
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 101 1e-22
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 74 3e-14
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 27 3.2
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.6
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.4
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 9.8
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 158 bits (384), Expect = 8e-40
Identities = 68/100 (68%), Positives = 82/100 (82%)
Frame = +1
Query: 214 FQHFLQRDRSWQARPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 393
F F + PR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHY
Sbjct: 49 FSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHY 108
Query: 394 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGLG 513
T+GKE+VD V D+IR++AD C+GLQGFL+FHSFGGGTG G
Sbjct: 109 TVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSG 148
Score = 139 bits (337), Expect = 4e-34
Identities = 60/81 (74%), Positives = 74/81 (91%)
Frame = +3
Query: 510 GFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNE 689
GF +LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVDNE
Sbjct: 148 GFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNE 207
Query: 690 AIYDICRRNLDIERPTYTNLN 752
+ YDICRRNLDIERP+Y NLN
Sbjct: 208 SCYDICRRNLDIERPSYENLN 228
Score = 99.5 bits (237), Expect = 5e-22
Identities = 43/63 (68%), Positives = 48/63 (76%)
Frame = +2
Query: 71 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 250
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 251 HDP 259
+ P
Sbjct: 61 YVP 63
Score = 36.7 bits (81), Expect = 0.004
Identities = 16/19 (84%), Positives = 19/19 (100%)
Frame = +2
Query: 764 QIVSSITASLRFDGALNVD 820
Q+VSSITASLRF+G+LNVD
Sbjct: 233 QVVSSITASLRFEGSLNVD 251
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 157 bits (380), Expect = 2e-39
Identities = 63/86 (73%), Positives = 80/86 (93%)
Frame = +1
Query: 256 PRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRI 435
PR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RI
Sbjct: 67 PRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERI 126
Query: 436 RKLADQCTGLQGFLIFHSFGGGTGLG 513
R++AD C+GLQGFL+FHSFGGGTG G
Sbjct: 127 RRMADNCSGLQGFLVFHSFGGGTGSG 152
Score = 144 bits (348), Expect = 2e-35
Identities = 62/81 (76%), Positives = 74/81 (91%)
Frame = +3
Query: 510 GFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNE 689
G +LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVDNE
Sbjct: 152 GLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNE 211
Query: 690 AIYDICRRNLDIERPTYTNLN 752
A YDICRRNLDIERPTY NLN
Sbjct: 212 ACYDICRRNLDIERPTYENLN 232
Score = 92.3 bits (219), Expect = 8e-20
Identities = 47/68 (69%), Positives = 49/68 (72%), Gaps = 5/68 (7%)
Frame = +2
Query: 71 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 235
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 236 TGAGKHDP 259
TG GK P
Sbjct: 60 TGQGKFVP 67
Score = 35.1 bits (77), Expect = 0.012
Identities = 16/19 (84%), Positives = 18/19 (94%)
Frame = +2
Query: 764 QIVSSITASLRFDGALNVD 820
Q+VSSITASLRF G+LNVD
Sbjct: 237 QVVSSITASLRFAGSLNVD 255
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 101 bits (242), Expect = 1e-22
Identities = 45/86 (52%), Positives = 59/86 (68%)
Frame = +1
Query: 256 PRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRI 435
PRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD +
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 436 RKLADQCTGLQGFLIFHSFGGGTGLG 513
R+ A+ C LQGF + HS GGGTG G
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSG 146
Score = 70.9 bits (166), Expect = 2e-13
Identities = 32/81 (39%), Positives = 50/81 (61%)
Frame = +3
Query: 510 GFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNE 689
G +LL+ ++ +Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +DNE
Sbjct: 146 GMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNE 205
Query: 690 AIYDICRRNLDIERPTYTNLN 752
A+ I L I+ P+Y +LN
Sbjct: 206 ALSSIFANTLKIKSPSYDDLN 226
Score = 41.5 bits (93), Expect = 1e-04
Identities = 21/63 (33%), Positives = 29/63 (46%)
Frame = +2
Query: 71 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 250
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 251 HDP 259
+ P
Sbjct: 59 YVP 61
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 73.7 bits (173), Expect = 3e-14
Identities = 36/88 (40%), Positives = 54/88 (61%), Gaps = 2/88 (2%)
Frame = +1
Query: 256 PRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKED--AANNYARGHYTIGKEIVDLVLD 429
PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++D
Sbjct: 62 PRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMD 120
Query: 430 RIRKLADQCTGLQGFLIFHSFGGGTGLG 513
I + AD L+GF + HS GGTG G
Sbjct: 121 MIDREADGSDSLEGFSLLHSIAGGTGSG 148
Score = 53.2 bits (122), Expect = 4e-08
Identities = 29/82 (35%), Positives = 44/82 (53%), Gaps = 1/82 (1%)
Frame = +3
Query: 510 GFTSLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMVDN 686
G S L+ERL+ Y KK ++++P Q VS VV+PYNS+L ++D ++DN
Sbjct: 148 GLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDN 207
Query: 687 EAIYDICRRNLDIERPTYTNLN 752
A+ I L + PT+ N
Sbjct: 208 AALAHIAADRLHTQNPTFHQQN 229
Score = 50.0 bits (114), Expect = 4e-07
Identities = 25/62 (40%), Positives = 36/62 (58%)
Frame = +2
Query: 74 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 253
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 254 DP 259
P
Sbjct: 61 IP 62
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 27.1 bits (57), Expect = 3.2
Identities = 12/38 (31%), Positives = 17/38 (44%)
Frame = -2
Query: 306 ADLINNSRFKIDEDSTGSCLPAPVSLKKVLKESSPPPM 193
AD+IN SR+ ID S + + L P P+
Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQPI 492
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 26.2 bits (55), Expect = 5.6
Identities = 15/36 (41%), Positives = 20/36 (55%)
Frame = +3
Query: 150 STASSLMARCPQTRPSGVETILSTLSSARPELASTT 257
ST SSL + ++PS T ST SSA P S++
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSSS 208
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.4
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 169 WPDAHRQDHRGWRRFFQHFLQR 234
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 9.8
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +3
Query: 60 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 182
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,318,979
Number of Sequences: 5004
Number of extensions: 69660
Number of successful extensions: 217
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 214
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 400438000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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