BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10453 (656 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 164 1e-41 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 63 3e-11 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 60 4e-10 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 42 6e-05 SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schiz... 28 1.0 SPBC14F5.06 |||iron-sulfur protein|Schizosaccharomyces pombe|chr... 27 1.8 SPAC694.02 |||DEAD/DEAH box helicase|Schizosaccharomyces pombe|c... 27 3.1 SPCC1259.06 |||transcription factor TFIID complex subunit 8 |Sch... 26 4.2 SPAC3H5.08c |||WD repeat protein Wdr44 family|Schizosaccharomyce... 26 5.5 SPBC13E7.06 |msd1|mug172|spindle pole body protein Msd1|Schizosa... 26 5.5 SPBC713.04c |||U3 snoRNP-associated protein Utp1|Schizosaccharom... 25 9.6 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 164 bits (398), Expect = 1e-41 Identities = 76/129 (58%), Positives = 96/129 (74%) Frame = +3 Query: 258 DPRHGRYLTVAAIFRGRMSMKEVDEQMLSIQNKNSSFFVEWIPNNVKTAVCDIPPKGLKM 437 DPRHGRYLTVAA+FRG++SMKEVDEQ+ S+Q KNS++FVEWIP+NV AVC +PPK LKM Sbjct: 304 DPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPKDLKM 363 Query: 438 SSTFIGNTTAIQELFKRISEQFSACSDVKLSCTGTPARAWTRWSSTKQRATSNDLVSEYQ 617 S+TFIGN+T+IQE+F+R+ +QFSA K T+ + NDLVSEYQ Sbjct: 364 SATFIGNSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQ 423 Query: 618 QYQEATAED 644 QYQEA ++ Sbjct: 424 QYQEAGIDE 432 Score = 80.2 bits (189), Expect = 2e-16 Identities = 35/49 (71%), Positives = 42/49 (85%) Frame = +1 Query: 103 QVAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELAQQMFDAKNMM 249 ++AVNMVPFPRLHFFM GFAPL + GS ++A++VPEL QQMFDA NMM Sbjct: 252 KLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANNMM 300 Score = 57.2 bits (132), Expect = 2e-09 Identities = 24/34 (70%), Positives = 29/34 (85%) Frame = +2 Query: 5 NPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRK 106 +P+Y DLNHLVS M+GVTT RFPG+LN+DLRK Sbjct: 219 SPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRK 252 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 63.3 bits (147), Expect = 3e-11 Identities = 29/92 (31%), Positives = 50/92 (54%), Gaps = 8/92 (8%) Frame = +3 Query: 252 RCDPRHGRYLTVAAIFRGRMSMKEVDEQMLSIQNKNSSFFVEWIPNNVKTAVCDIPPKGL 431 +CDPR GRY+ ++RG + ++V + +I+ K + FV+W P K +CD PP+ + Sbjct: 304 KCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHI 363 Query: 432 KMSS--------TFIGNTTAIQELFKRISEQF 503 + S + NTT+I E + R+ +F Sbjct: 364 EGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKF 395 Score = 43.2 bits (97), Expect = 3e-05 Identities = 15/28 (53%), Positives = 23/28 (82%) Frame = +2 Query: 509 MFRRKAFLHWYTGEGMDEMEFNEAESNV 592 M+ ++AF+HWY GEGM+E EF+EA ++ Sbjct: 398 MYSKRAFVHWYVGEGMEEGEFSEAREDL 425 Score = 41.9 bits (94), Expect = 8e-05 Identities = 17/50 (34%), Positives = 29/50 (58%) Frame = +1 Query: 100 AQVAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELAQQMFDAKNMM 249 A+ N+VP+PR+HF + +AP+ S + + +V E+ Q F+ N M Sbjct: 253 AEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQM 302 Score = 39.9 bits (89), Expect = 3e-04 Identities = 16/40 (40%), Positives = 26/40 (65%) Frame = +2 Query: 8 PTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKWRSTWCP 127 P+Y +LN L++ +S +T LRF G LN DL ++++ P Sbjct: 222 PSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVP 261 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 59.7 bits (138), Expect = 4e-10 Identities = 28/92 (30%), Positives = 49/92 (53%), Gaps = 8/92 (8%) Frame = +3 Query: 252 RCDPRHGRYLTVAAIFRGRMSMKEVDEQMLSIQNKNSSFFVEWIPNNVKTAVCDIPPKGL 431 +CDPR GRY+ ++RG + ++V + SI+++ + FV+W P K +C PP+ + Sbjct: 308 KCDPRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHV 367 Query: 432 KMSS--------TFIGNTTAIQELFKRISEQF 503 S + NTT+I E + R+ +F Sbjct: 368 PGSGIAKVNRAVCMLSNTTSIAEAWSRLDHKF 399 Score = 43.2 bits (97), Expect = 3e-05 Identities = 15/28 (53%), Positives = 23/28 (82%) Frame = +2 Query: 509 MFRRKAFLHWYTGEGMDEMEFNEAESNV 592 M+ ++AF+HWY GEGM+E EF+EA ++ Sbjct: 402 MYSKRAFVHWYVGEGMEEGEFSEAREDL 429 Score = 41.9 bits (94), Expect = 8e-05 Identities = 17/40 (42%), Positives = 26/40 (65%) Frame = +2 Query: 8 PTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKWRSTWCP 127 PTY +LN L++ +S +T LRF G LN DL ++++ P Sbjct: 226 PTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVP 265 Score = 39.1 bits (87), Expect = 6e-04 Identities = 15/45 (33%), Positives = 27/45 (60%) Frame = +1 Query: 115 NMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELAQQMFDAKNMM 249 N+VP+PR+HF + ++P+ S + + +V E+ Q F+ N M Sbjct: 262 NLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQM 306 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 42.3 bits (95), Expect = 6e-05 Identities = 21/45 (46%), Positives = 26/45 (57%) Frame = +2 Query: 5 NPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKWRSTWCPSRVC 139 NPT+ N LVS MS TT LR+PG +N DL ++ PS C Sbjct: 222 NPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRC 266 Score = 32.3 bits (70), Expect = 0.063 Identities = 13/51 (25%), Positives = 30/51 (58%), Gaps = 3/51 (5%) Frame = +1 Query: 106 VAVNMVPFPRLHFFMPGFAPLTSRGSQQYRAL---TVPELAQQMFDAKNMM 249 + +++P PR HF + + P T++ ++ +A+ TV ++ +++ KN M Sbjct: 256 IIASLIPSPRCHFLLTSYTPFTNQQVEEAKAIRKTTVLDVMRRLLLPKNQM 306 >SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1253 Score = 28.3 bits (60), Expect = 1.0 Identities = 12/27 (44%), Positives = 18/27 (66%) Frame = -3 Query: 633 WPPGTVGTQTPDRLTLLSASLNSISSM 553 W GT+ QTPD ++L S SL++ S+ Sbjct: 1096 WMEGTMEDQTPDDISLASTSLSTNRSL 1122 >SPBC14F5.06 |||iron-sulfur protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 593 Score = 27.5 bits (58), Expect = 1.8 Identities = 15/44 (34%), Positives = 22/44 (50%) Frame = -2 Query: 421 GGMSHTAVFTLLGIHSTKKLLFLFWMESICSSTSFMDMRPRKMA 290 GG S + LLG + T K F WM +S + M+P+ +A Sbjct: 370 GGFSDAEIIVLLGENGTGKTTFCKWMAK--NSDLKISMKPQTIA 411 >SPAC694.02 |||DEAD/DEAH box helicase|Schizosaccharomyces pombe|chr 1|||Manual Length = 1717 Score = 26.6 bits (56), Expect = 3.1 Identities = 16/69 (23%), Positives = 30/69 (43%) Frame = +1 Query: 448 LSGTRQPSRNYSREYPSSSLHVQT*SFLALVHRRGHGRDGVQRSREQRQTIWCLSTNSTR 627 LS TR+ N ++ + Q F++ + + G + + +RQTI+ NST+ Sbjct: 448 LSATRKTGDNTKDRKQANKVRRQDQFFISHIQKYASSLTGAKGGQLKRQTIFTQGPNSTK 507 Query: 628 RPPRKTTTE 654 + E Sbjct: 508 ESKQAVKEE 516 >SPCC1259.06 |||transcription factor TFIID complex subunit 8 |Schizosaccharomyces pombe|chr 3|||Manual Length = 222 Score = 26.2 bits (55), Expect = 4.2 Identities = 14/44 (31%), Positives = 21/44 (47%), Gaps = 1/44 (2%) Frame = +1 Query: 85 AERRPAQVAVNMVPFPRLHFFM-PGFAPLTSRGSQQYRALTVPE 213 ++ RPA V ++ PFP H +M P+ +Q R L E Sbjct: 123 SQNRPAYVVNHLPPFPASHTYMATPVYPVRPTSPKQIRELATQE 166 >SPAC3H5.08c |||WD repeat protein Wdr44 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 855 Score = 25.8 bits (54), Expect = 5.5 Identities = 16/41 (39%), Positives = 25/41 (60%) Frame = +3 Query: 333 QMLSIQNKNSSFFVEWIPNNVKTAVCDIPPKGLKMSSTFIG 455 ++ SI+ K SF+ E +P + TAV P GL ++ TF+G Sbjct: 375 RLWSIKEKAVSFWNE-LPELI-TAVAFSPDGGLAIAGTFVG 413 >SPBC13E7.06 |msd1|mug172|spindle pole body protein Msd1|Schizosaccharomyces pombe|chr 2|||Manual Length = 331 Score = 25.8 bits (54), Expect = 5.5 Identities = 11/20 (55%), Positives = 15/20 (75%) Frame = -3 Query: 594 LTLLSASLNSISSMPSPVYQ 535 LT LS +LNS+ M SP+Y+ Sbjct: 234 LTCLSGTLNSLVEMLSPLYE 253 >SPBC713.04c |||U3 snoRNP-associated protein Utp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 854 Score = 25.0 bits (52), Expect = 9.6 Identities = 8/22 (36%), Positives = 15/22 (68%) Frame = +2 Query: 476 TIQENIRAVLCMFRRKAFLHWY 541 ++ E R +LC F R++ LH++ Sbjct: 72 SVDEEGRCILCNFLRRSVLHYF 93 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,511,498 Number of Sequences: 5004 Number of extensions: 48697 Number of successful extensions: 197 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 168 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 195 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 297805304 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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