BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10446 (779 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 137 1e-33 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 137 1e-33 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 101 1e-22 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 90 3e-19 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 42 7e-05 SPBC146.09c |lsd1|swm1, saf110|histone demethylase SWIRM1|Schizo... 29 0.75 SPAC3C7.09 |set8||lysine methyltransferase Set8 |Schizosaccharom... 28 1.3 SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+, L-... 27 2.3 SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces pom... 27 2.3 SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein |Schizo... 26 5.3 SPAC4A8.06c |||esterase/lipase |Schizosaccharomyces pombe|chr 1|... 26 5.3 SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit ... 26 7.0 SPAC23H4.16c |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 9.2 SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 25 9.2 SPAC11D3.15 |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||M... 25 9.2 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 137 bits (332), Expect = 1e-33 Identities = 65/101 (64%), Positives = 73/101 (72%) Frame = +3 Query: 3 CFGPEGTGPNILVDCSKGVQYLNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLH 182 CFGP+ TG N++VD +K V YLNEIKDSVVA F WA+KEG M EENLR RFNI DV LH Sbjct: 635 CFGPDTTGANVVVDQTKAVAYLNEIKDSVVAAFAWASKEGPMFEENLRSCRFNILDVVLH 694 Query: 183 TDAIHRGGGQIIPTTRRCLYACLLLLSPVSWSLYIFVKFSV 305 DAIHRGGGQIIPT RR +YA LL SP+ V+ V Sbjct: 695 ADAIHRGGGQIIPTARRVVYASTLLASPIIQEPVFLVEIQV 735 Score = 125 bits (302), Expect = 6e-30 Identities = 55/91 (60%), Positives = 68/91 (74%) Frame = +2 Query: 230 KMLVCMSATAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKA 409 +++ + A P + EPV+L EIQ E A+GGIY VLN++RGHVF E Q GTP++ +KA Sbjct: 711 RVVYASTLLASPIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNIKA 770 Query: 410 YLPVNESFGFTADLRSNTGGQAFPQCVFDHW 502 YLPVNESFGFT +LR T GQAFPQ VFDHW Sbjct: 771 YLPVNESFGFTGELRQATAGQAFPQLVFDHW 801 Score = 41.5 bits (93), Expect = 1e-04 Identities = 17/30 (56%), Positives = 22/30 (73%) Frame = +1 Query: 508 LPGEPCEPQSKPYNVVQETRKRKGLKEGLP 597 + G+P +P SKP +V E RKRKGLKE +P Sbjct: 804 MSGDPLDPTSKPGQIVCEARKRKGLKENVP 833 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 137 bits (332), Expect = 1e-33 Identities = 65/101 (64%), Positives = 73/101 (72%) Frame = +3 Query: 3 CFGPEGTGPNILVDCSKGVQYLNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLH 182 CFGP+ TG N++VD +K V YLNEIKDSVVA F WA+KEG M EENLR RFNI DV LH Sbjct: 635 CFGPDTTGANVVVDQTKAVAYLNEIKDSVVAAFAWASKEGPMFEENLRSCRFNILDVVLH 694 Query: 183 TDAIHRGGGQIIPTTRRCLYACLLLLSPVSWSLYIFVKFSV 305 DAIHRGGGQIIPT RR +YA LL SP+ V+ V Sbjct: 695 ADAIHRGGGQIIPTARRVVYASTLLASPIIQEPVFLVEIQV 735 Score = 125 bits (302), Expect = 6e-30 Identities = 55/91 (60%), Positives = 68/91 (74%) Frame = +2 Query: 230 KMLVCMSATAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKA 409 +++ + A P + EPV+L EIQ E A+GGIY VLN++RGHVF E Q GTP++ +KA Sbjct: 711 RVVYASTLLASPIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNIKA 770 Query: 410 YLPVNESFGFTADLRSNTGGQAFPQCVFDHW 502 YLPVNESFGFT +LR T GQAFPQ VFDHW Sbjct: 771 YLPVNESFGFTGELRQATAGQAFPQLVFDHW 801 Score = 41.5 bits (93), Expect = 1e-04 Identities = 17/30 (56%), Positives = 22/30 (73%) Frame = +1 Query: 508 LPGEPCEPQSKPYNVVQETRKRKGLKEGLP 597 + G+P +P SKP +V E RKRKGLKE +P Sbjct: 804 MSGDPLDPTSKPGQIVCEARKRKGLKENVP 833 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 101 bits (242), Expect = 1e-22 Identities = 48/92 (52%), Positives = 64/92 (69%), Gaps = 2/92 (2%) Frame = +2 Query: 239 VCMSA--TAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAY 412 VC S+ TA PRLMEPVY+ E+ P ++ IY +L RRRGHV ++ G+P+++V+A Sbjct: 828 VCYSSFLTASPRLMEPVYMVEVHAPADSLPIIYDLLTRRRGHVLQDIPRPGSPLYLVRAL 887 Query: 413 LPVNESFGFTADLRSNTGGQAFPQCVFDHWQV 508 +PV +S GF DLR +T GQA Q VFDHWQV Sbjct: 888 IPVIDSCGFETDLRVHTQGQAMCQMVFDHWQV 919 Score = 83.4 bits (197), Expect = 3e-17 Identities = 40/91 (43%), Positives = 53/91 (58%), Gaps = 4/91 (4%) Frame = +3 Query: 6 FGPEGTGPNILVDCSKGVQY----LNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDV 173 FGP+ G NIL D + LN +K+ + GFQW +EG + +E +R V F + DV Sbjct: 748 FGPDDRGTNILRDDTLSTDVDKNVLNSVKEYIKQGFQWGTREGPLCDETIRNVNFRLMDV 807 Query: 174 TLHTDAIHRGGGQIIPTTRRCLYACLLLLSP 266 L + I+RGGGQIIPT RR Y+ L SP Sbjct: 808 VLAPEQIYRGGGQIIPTARRVCYSSFLTASP 838 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 90.2 bits (214), Expect = 3e-19 Identities = 39/86 (45%), Positives = 56/86 (65%) Frame = +2 Query: 263 PRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFT 442 PRLM +Y C++Q +G +YGV+++RRG V +E GTP FIVKA +PV ESFGF Sbjct: 860 PRLMLAMYSCDVQATSEVLGRVYGVVSKRRGRVIDEEMKEGTPFFIVKALIPVVESFGFA 919 Query: 443 ADLRSNTGGQAFPQCVFDHWQVSLEN 520 ++ T G A+PQ +F +++ EN Sbjct: 920 VEILKRTSGAAYPQLIFHGFEMLDEN 945 Score = 29.5 bits (63), Expect = 0.57 Identities = 19/73 (26%), Positives = 35/73 (47%), Gaps = 11/73 (15%) Frame = +3 Query: 6 FGPEGTGPNILVDCSKGVQYL-----------NEIKDSVVAGFQWAAKEGVMAEENLRGV 152 FGP+ GPNIL D +K ++ +++ + VV FQ +G + E ++G+ Sbjct: 754 FGPKRVGPNILFDKTKKMRDFRRQSDETKLIPSDLSEYVVTAFQLITHQGPLCAEPVQGI 813 Query: 153 RFNIYDVTLHTDA 191 +I + D+ Sbjct: 814 CVSIDQFDISDDS 826 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 42.3 bits (95), Expect = 7e-05 Identities = 20/72 (27%), Positives = 39/72 (54%) Frame = +2 Query: 257 AQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFG 436 A P ++EP+ I P GG+ G L++R+ + + F ++A +P+N F Sbjct: 670 ANPMVLEPIMNVSITAPVEHQGGVIGNLDKRKATIVDSD--TDEDEFTLQAEVPLNSMFS 727 Query: 437 FTADLRSNTGGQ 472 +++D+R+ T G+ Sbjct: 728 YSSDIRALTKGK 739 >SPBC146.09c |lsd1|swm1, saf110|histone demethylase SWIRM1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1000 Score = 29.1 bits (62), Expect = 0.75 Identities = 13/37 (35%), Positives = 20/37 (54%), Gaps = 1/37 (2%) Frame = +3 Query: 456 PTPADRPSRSAYSTIGRSPWRTVRT-SEQALQRCTGN 563 P P RPS + Y ++ W +T ++Q QR TG+ Sbjct: 837 PVPPSRPSANPYLLYQKTQWHVCKTLADQDKQRVTGD 873 >SPAC3C7.09 |set8||lysine methyltransferase Set8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 429 Score = 28.3 bits (60), Expect = 1.3 Identities = 13/38 (34%), Positives = 20/38 (52%) Frame = +3 Query: 438 LLPICVPTPADRPSRSAYSTIGRSPWRTVRTSEQALQR 551 +LP+ + TPA P + YS G S + V + LQ+ Sbjct: 109 VLPLSINTPAQWPEKEVYSLQGTSIFNPVCVKRKILQQ 146 >SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+, L-lysine forming] |Schizosaccharomyces pombe|chr 1|||Manual Length = 368 Score = 27.5 bits (58), Expect = 2.3 Identities = 14/40 (35%), Positives = 22/40 (55%) Frame = -2 Query: 226 VVGMIWPPPLWMASVWSVTS*MLNRTPRKFSSAITPSLAA 107 V G+ PPPL + S+ + + + + FS A+ PSL A Sbjct: 305 VKGVTTPPPLEVISIDHLPTLLPRESSEAFSEALIPSLLA 344 >SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces pombe|chr 1|||Manual Length = 166 Score = 27.5 bits (58), Expect = 2.3 Identities = 13/47 (27%), Positives = 23/47 (48%) Frame = -2 Query: 202 PLWMASVWSVTS*MLNRTPRKFSSAITPSLAAHWNPATTESLISLRY 62 P W+A V ++ S + P FSS + +L A+ N + + + Y Sbjct: 43 PFWLAEVLAINSFVSIHMPAPFSSVVRNALKANPNSVSIRDITTHYY 89 >SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 391 Score = 26.2 bits (55), Expect = 5.3 Identities = 18/54 (33%), Positives = 25/54 (46%) Frame = -1 Query: 299 EFHKDIQAP*DGAEQ*QTCIQASSSCWNDLATTSMDGISMECYIIDVESNTTQI 138 E K IQ GA Q + CW++L+TTS + + II + TT I Sbjct: 187 ELAKQIQETSLGATT-QEDESSDGICWDELSTTSPESSKVSEPIIQDNTQTTHI 239 >SPAC4A8.06c |||esterase/lipase |Schizosaccharomyces pombe|chr 1|||Manual Length = 578 Score = 26.2 bits (55), Expect = 5.3 Identities = 17/43 (39%), Positives = 25/43 (58%), Gaps = 3/43 (6%) Frame = +2 Query: 209 PNHSNN*KMLVCMSATAQPRLMEPVYLCEIQC---PEVAVGGI 328 P+H+N + + +S + RLMEP EIQC P+ A+G I Sbjct: 469 PSHANEDMVRLRISRNGEERLMEP--KDEIQCLNLPKSALGTI 509 >SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit Sec63 |Schizosaccharomyces pombe|chr 2|||Manual Length = 611 Score = 25.8 bits (54), Expect = 7.0 Identities = 17/79 (21%), Positives = 34/79 (43%) Frame = +2 Query: 203 WWPNHSNN*KMLVCMSATAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVA 382 W+P + + +S A + + + E E + ++ LNR++ + F Q+ Sbjct: 234 WFPKMETSLTLDELLSLFASSKELTSLVPNEKNPKEYILKLLFDHLNRKKTNNFNTHQIL 293 Query: 383 GTPMFIVKAYLPVNESFGF 439 ++ A L V +FGF Sbjct: 294 SQSDVVLNALLSVATAFGF 312 >SPAC23H4.16c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 328 Score = 25.4 bits (53), Expect = 9.2 Identities = 19/63 (30%), Positives = 30/63 (47%), Gaps = 7/63 (11%) Frame = -1 Query: 242 IQASSSCWND-----LAT--TSMDGISMECYIIDVESNTTQILFSHNSFLSGPLESSHNR 84 +Q S S +ND LA ++ + + Y++ E + Q + SFL L S HN+ Sbjct: 203 LQHSDSSFNDKFLLLLANIPVTVSNVEVLLYLLQQEESLAQFDLNGKSFLYHMLVSLHNQ 262 Query: 83 VLN 75 V N Sbjct: 263 VTN 265 >SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 3131 Score = 25.4 bits (53), Expect = 9.2 Identities = 13/51 (25%), Positives = 25/51 (49%) Frame = +2 Query: 392 MFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVSLENRANLRASP 544 +F+ ++ + T D SN+ + ++D ++VSLE+ A L P Sbjct: 654 LFVDAGFISITSQNVETNDKESNSESETLYHLIYDRYRVSLES-ARLLLGP 703 >SPAC11D3.15 |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1317 Score = 25.4 bits (53), Expect = 9.2 Identities = 11/31 (35%), Positives = 18/31 (58%) Frame = +3 Query: 63 YLNEIKDSVVAGFQWAAKEGVMAEENLRGVR 155 YL+ + +AGFQ G+ ++N +GVR Sbjct: 248 YLSPVVRRYLAGFQSGFLHGLKTKDNSKGVR 278 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,370,594 Number of Sequences: 5004 Number of extensions: 73934 Number of successful extensions: 202 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 183 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 200 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 377352472 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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