Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV10446
(779 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 139 2e-33
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 105 2e-23
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 105 2e-23
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 103 1e-22
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 82 5e-16
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 53 2e-07
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 53 2e-07
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 48 7e-06
At5g13650.2 68418.m01585 elongation factor family protein contai... 40 0.002
At5g13650.1 68418.m01584 elongation factor family protein contai... 40 0.002
At3g61740.1 68416.m06923 PHD finger family protein (ATX3) contai... 32 0.37
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 31 1.1
At5g61380.1 68418.m07701 ABI3-interacting protein 1 (AIP1) ident... 29 3.5
At5g62170.1 68418.m07804 expressed protein various predicted pro... 29 4.6
At2g25730.1 68415.m03084 expressed protein 29 4.6
At5g56050.1 68418.m06993 hypothetical protein 28 8.0
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 139 bits (336), Expect = 2e-33
Identities = 61/87 (70%), Positives = 72/87 (82%)
Frame = +3
Query: 6 FGPEGTGPNILVDCSKGVQYLNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHT 185
FGPE TGPN++VD KGVQYLNEIKDSVVAGFQWA+KEG +AEEN+RG+ F + DV LH+
Sbjct: 637 FGPETTGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGPLAEENMRGICFEVCDVVLHS 696
Query: 186 DAIHRGGGQIIPTTRRCLYACLLLLSP 266
DAIHRGGGQ+IPT RR +YA + P
Sbjct: 697 DAIHRGGGQVIPTARRVIYASQITAKP 723
Score = 138 bits (334), Expect = 4e-33
Identities = 59/93 (63%), Positives = 76/93 (81%)
Frame = +2
Query: 230 KMLVCMSATAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKA 409
+++ TA+PRL+EPVY+ EIQ PE A+GGIY VLN++RGHVFEE Q GTP++ +KA
Sbjct: 712 RVIYASQITAKPRLLEPVYMVEIQAPEGALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKA 771
Query: 410 YLPVNESFGFTADLRSNTGGQAFPQCVFDHWQV 508
YLPV ESFGF++ LR+ T GQAFPQCVFDHW++
Sbjct: 772 YLPVVESFGFSSQLRAATSGQAFPQCVFDHWEM 804
Score = 29.1 bits (62), Expect = 3.5
Identities = 12/29 (41%), Positives = 19/29 (65%)
Frame = +1
Query: 508 LPGEPCEPQSKPYNVVQETRKRKGLKEGL 594
+ +P EP ++ +V + RKRKGLKE +
Sbjct: 805 MSSDPLEPGTQASVLVADIRKRKGLKEAM 833
>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 105 bits (253), Expect = 2e-23
Identities = 52/84 (61%), Positives = 58/84 (69%)
Frame = +2
Query: 257 AQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFG 436
A PRLMEPVY EIQ P V IY VL+RRRGHV + GTP +IVKA+LPV ESFG
Sbjct: 837 ATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFG 896
Query: 437 FTADLRSNTGGQAFPQCVFDHWQV 508
F DLR +T GQAF VFDHW +
Sbjct: 897 FETDLRYHTQGQAFCLSVFDHWAI 920
Score = 87.8 bits (208), Expect = 7e-18
Identities = 39/91 (42%), Positives = 58/91 (63%), Gaps = 4/91 (4%)
Frame = +3
Query: 6 FGPEGTGPNILVDCSKGVQY----LNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDV 173
FGP+ GPNIL+D + + + +KDS+V GFQW A+EG + +E +R V+F I D
Sbjct: 749 FGPDKQGPNILLDDTLPTEVDRNLMMAVKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDA 808
Query: 174 TLHTDAIHRGGGQIIPTTRRCLYACLLLLSP 266
+ + +HRG GQ+IPT RR Y+ L+ +P
Sbjct: 809 RIAPEPLHRGSGQMIPTARRVAYSAFLMATP 839
>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 105 bits (253), Expect = 2e-23
Identities = 52/84 (61%), Positives = 58/84 (69%)
Frame = +2
Query: 257 AQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFG 436
A PRLMEPVY EIQ P V IY VL+RRRGHV + GTP +IVKA+LPV ESFG
Sbjct: 837 ATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFG 896
Query: 437 FTADLRSNTGGQAFPQCVFDHWQV 508
F DLR +T GQAF VFDHW +
Sbjct: 897 FETDLRYHTQGQAFCLSVFDHWAI 920
Score = 87.8 bits (208), Expect = 7e-18
Identities = 39/91 (42%), Positives = 58/91 (63%), Gaps = 4/91 (4%)
Frame = +3
Query: 6 FGPEGTGPNILVDCSKGVQY----LNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDV 173
FGP+ GPNIL+D + + + +KDS+V GFQW A+EG + +E +R V+F I D
Sbjct: 749 FGPDKQGPNILLDDTLPTEVDRNLMMAVKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDA 808
Query: 174 TLHTDAIHRGGGQIIPTTRRCLYACLLLLSP 266
+ + +HRG GQ+IPT RR Y+ L+ +P
Sbjct: 809 RIAPEPLHRGSGQMIPTARRVAYSAFLMATP 839
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 103 bits (247), Expect = 1e-22
Identities = 51/84 (60%), Positives = 58/84 (69%)
Frame = +2
Query: 257 AQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFG 436
A PRLMEPVY EIQ P V IY VL+RRRG+V + GTP +IVKA+LPV ESFG
Sbjct: 823 ATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGYVTSDVPQPGTPAYIVKAFLPVIESFG 882
Query: 437 FTADLRSNTGGQAFPQCVFDHWQV 508
F DLR +T GQAF VFDHW +
Sbjct: 883 FETDLRYHTQGQAFCLSVFDHWAI 906
Score = 84.2 bits (199), Expect = 9e-17
Identities = 38/91 (41%), Positives = 57/91 (62%), Gaps = 4/91 (4%)
Frame = +3
Query: 6 FGPEGTGPNILVDCSKGVQY----LNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDV 173
FGP+ G NIL+D + + + +KDS+V GFQW A+EG + +E +R V+F I D
Sbjct: 735 FGPDKQGTNILLDDTLPTEVDRNLMMGVKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDA 794
Query: 174 TLHTDAIHRGGGQIIPTTRRCLYACLLLLSP 266
+ + +HRG GQ+IPT RR Y+ L+ +P
Sbjct: 795 RIAPEPLHRGSGQMIPTARRVAYSAFLMATP 825
>At3g22980.1 68416.m02898 elongation factor Tu family protein similar
to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 81.8 bits (193), Expect = 5e-16
Identities = 38/95 (40%), Positives = 56/95 (58%), Gaps = 2/95 (2%)
Frame = +2
Query: 242 CMSATAQ--PRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYL 415
C +A Q PR++E +Y CE+ +G +Y VL+RRR + +E G+ +F V AY+
Sbjct: 863 CRAAVLQTNPRIVEAMYFCELNTAPEYLGPMYAVLSRRRARILKEEMQEGSSLFTVHAYV 922
Query: 416 PVNESFGFTADLRSNTGGQAFPQCVFDHWQVSLEN 520
PV+ESFGF +LR T G A V HW++ E+
Sbjct: 923 PVSESFGFADELRKGTSGGASALMVLSHWEMLEED 957
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 53.2 bits (122), Expect = 2e-07
Identities = 33/99 (33%), Positives = 54/99 (54%), Gaps = 1/99 (1%)
Frame = +2
Query: 239 VCMSATAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLP 418
+C +A A+P ++EPV L E++ P G + G +N+R+G + Q ++ A +P
Sbjct: 648 LCYTA-ARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVP 704
Query: 419 VNESFGFTADLRSNTGGQA-FPQCVFDHWQVSLENRANL 532
+N FG++ LRS T G+ F +H VS E +A L
Sbjct: 705 LNNMFGYSTSLRSMTQGKGEFTMEYKEHSAVSNEVQAQL 743
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 53.2 bits (122), Expect = 2e-07
Identities = 33/99 (33%), Positives = 54/99 (54%), Gaps = 1/99 (1%)
Frame = +2
Query: 239 VCMSATAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLP 418
+C +A A+P ++EPV L E++ P G + G +N+R+G + Q ++ A +P
Sbjct: 648 LCYTA-ARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVP 704
Query: 419 VNESFGFTADLRSNTGGQA-FPQCVFDHWQVSLENRANL 532
+N FG++ LRS T G+ F +H VS E +A L
Sbjct: 705 LNNMFGYSTSLRSMTQGKGEFTMEYKEHSAVSNEVQAQL 743
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 48.0 bits (109), Expect = 7e-06
Identities = 25/73 (34%), Positives = 40/73 (54%)
Frame = +2
Query: 257 AQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFG 436
A PR++EP+ E+ PE +G + G LN RRG + G + +V + +P+ E F
Sbjct: 683 AGPRMLEPIMRVEVVTPEEHLGDVIGDLNSRRGQINSFGDKPG-GLKVVDSLVPLAEMFQ 741
Query: 437 FTADLRSNTGGQA 475
+ + LR T G+A
Sbjct: 742 YVSTLRGMTKGRA 754
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 39.5 bits (88), Expect = 0.002
Identities = 19/79 (24%), Positives = 37/79 (46%)
Frame = +2
Query: 266 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTA 445
+L+EP + ++ PE +G + +L +RRG +F+ V ++ +P G
Sbjct: 473 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRN 532
Query: 446 DLRSNTGGQAFPQCVFDHW 502
+ + + G A VFD +
Sbjct: 533 AILTASRGTAILNTVFDSY 551
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 39.5 bits (88), Expect = 0.002
Identities = 19/79 (24%), Positives = 37/79 (46%)
Frame = +2
Query: 266 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTA 445
+L+EP + ++ PE +G + +L +RRG +F+ V ++ +P G
Sbjct: 472 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRN 531
Query: 446 DLRSNTGGQAFPQCVFDHW 502
+ + + G A VFD +
Sbjct: 532 AILTASRGTAILNTVFDSY 550
>At3g61740.1 68416.m06923 PHD finger family protein (ATX3) contains
Pfam domains PF00628: PHD-finger and PF00855: PWWP
domain; identical to cDNA trithorax 3 (ATX3) partial cds
GI:15217142
Length = 799
Score = 32.3 bits (70), Expect = 0.37
Identities = 15/57 (26%), Positives = 26/57 (45%), Gaps = 4/57 (7%)
Frame = +1
Query: 124 LWLKRICVVF-DSTSMM*HSILMPSI---EVVAKSFQQLEDACMHVCYCSAPSHGAC 282
LW+ C F + H + P++ ++ A SF + +C+H C C+ H C
Sbjct: 565 LWVHVTCAWFRPEVGFLNHENMEPAVGLFKIPANSFLKTHGSCVHCCKCATHFHAMC 621
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 30.7 bits (66), Expect = 1.1
Identities = 21/68 (30%), Positives = 32/68 (47%), Gaps = 1/68 (1%)
Frame = +2
Query: 275 EPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNE-SFGFTADL 451
EP + I P VG + + + RRG E + + +F+ K LP+ E F +L
Sbjct: 466 EPTVIATIILPSEYVGAVINLCSDRRGQQLEYTFIDAQRVFL-KYQLPLREIVVDFYDEL 524
Query: 452 RSNTGGQA 475
+S T G A
Sbjct: 525 KSITSGYA 532
>At5g61380.1 68418.m07701 ABI3-interacting protein 1 (AIP1)
identical to pseudo-response regulator 1 GI:7576354 from
[Arabidopsis thaliana]; timing of CAB expression 1
protein (TOC1) GI:9247019; contains Pfam profile
PF00072: Response regulator receiver domain; identical
to cDNA ABI3-interacting protein 1 (aip1 gene)
GI:6996312
Length = 618
Score = 29.1 bits (62), Expect = 3.5
Identities = 14/49 (28%), Positives = 26/49 (53%)
Frame = -2
Query: 622 ICPNIELSLGDLPSILSSFSFPVQRCRACSEVRTVLQGDLPMVEYALRE 476
+C N SLG++ ++LS S+ V ++ +V L + P ++ L E
Sbjct: 23 LCDNDSTSLGEVFTLLSECSYQVTAVKSARQVIDALNAEGPDIDIILAE 71
>At5g62170.1 68418.m07804 expressed protein various predicted
proteins, Arabidopsis thaliana
Length = 703
Score = 28.7 bits (61), Expect = 4.6
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = -2
Query: 562 FPVQRCRACSEVRTVLQGDLPMVEYALREGLSAGVGTQI 446
FP+ +C ++ ++ GD P +E A E G+ T+I
Sbjct: 602 FPLAKCLVLEDIDALVAGDFPEIESAFEED-GEGIVTEI 639
>At2g25730.1 68415.m03084 expressed protein
Length = 2464
Score = 28.7 bits (61), Expect = 4.6
Identities = 16/39 (41%), Positives = 19/39 (48%)
Frame = +1
Query: 7 LAPRVPAPTSWSIAPKEFSTSMKLRTLLWLDSSGPLRKE 123
L P V A W + P + +T KL LLW S LR E
Sbjct: 425 LRPLV-ASMGWDLLPGKTATRRKLMRLLWTSDSQALRLE 462
>At5g56050.1 68418.m06993 hypothetical protein
Length = 283
Score = 27.9 bits (59), Expect = 8.0
Identities = 18/48 (37%), Positives = 22/48 (45%)
Frame = +3
Query: 462 PADRPSRSAYSTIGRSPWRTVRTSEQALQRCTGNEKEERIEGRSPRLN 605
P + PS YS I +PWRT S Q+ T E I +SP N
Sbjct: 19 PWETPSSKWYSPI-YTPWRTTPRSTQSTPTTTPIALTEVIVSKSPLSN 65
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,855,181
Number of Sequences: 28952
Number of extensions: 399994
Number of successful extensions: 1022
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 987
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1017
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1746037600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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