Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV10441
(784 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 149 3e-37
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 149 3e-37
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 149 3e-37
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 90 4e-19
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 79 5e-16
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 55 1e-08
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 29 0.99
SPAC17C9.06 |sam50||SAM complex subunit Sam50 |Schizosaccharomyc... 29 0.99
SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr 1|||Ma... 28 1.3
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 4.0
SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 26 5.3
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 7.0
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 26 7.0
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 149 bits (362), Expect = 3e-37
Identities = 65/87 (74%), Positives = 74/87 (85%)
Frame = +3
Query: 249 PSAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDK 428
P V FVP+SG+ GDNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPARPTDK
Sbjct: 183 PKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDK 242
Query: 429 PLRLPLQDVYKIGGIGTVPVGRVETGV 509
PLRLPLQDVYKIGGIGTVPVGRVETGV
Sbjct: 243 PLRLPLQDVYKIGGIGTVPVGRVETGV 269
Score = 135 bits (326), Expect = 8e-33
Identities = 64/84 (76%), Positives = 75/84 (89%)
Frame = +1
Query: 1 MITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPP 180
MITGTSQADCA+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T
Sbjct: 102 MITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--G 159
Query: 181 YSEPRFEEIKKEVSSYIKKIGYNP 252
+S+ RFEEI KE S++IKK+G+NP
Sbjct: 160 WSQARFEEIVKETSNFIKKVGFNP 183
Score = 123 bits (296), Expect = 3e-29
Identities = 56/92 (60%), Positives = 65/92 (70%)
Frame = +2
Query: 509 LKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDXXXXXXXXXXXXELRRGYVAGDSKN 688
+KPG IV FAPA +TTEVKSVEMHHE+L +PGD ++RRG V GDSKN
Sbjct: 270 IKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKN 329
Query: 689 NPPKGAADFTAQVIVLNHPGQNLKRYTPVLDC 784
+PP G A FTAQVI+LNHPGQ Y+PVLDC
Sbjct: 330 DPPMGCASFTAQVIILNHPGQISAGYSPVLDC 361
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 149 bits (362), Expect = 3e-37
Identities = 65/87 (74%), Positives = 74/87 (85%)
Frame = +3
Query: 249 PSAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDK 428
P V FVP+SG+ GDNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPARPTDK
Sbjct: 183 PKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDK 242
Query: 429 PLRLPLQDVYKIGGIGTVPVGRVETGV 509
PLRLPLQDVYKIGGIGTVPVGRVETGV
Sbjct: 243 PLRLPLQDVYKIGGIGTVPVGRVETGV 269
Score = 135 bits (327), Expect = 6e-33
Identities = 65/84 (77%), Positives = 75/84 (89%)
Frame = +1
Query: 1 MITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPP 180
MITGTSQADCAVLI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T
Sbjct: 102 MITGTSQADCAVLIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--G 159
Query: 181 YSEPRFEEIKKEVSSYIKKIGYNP 252
+S+ RFEEI KE S++IKK+G+NP
Sbjct: 160 WSQARFEEIVKETSNFIKKVGFNP 183
Score = 123 bits (296), Expect = 3e-29
Identities = 56/92 (60%), Positives = 65/92 (70%)
Frame = +2
Query: 509 LKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDXXXXXXXXXXXXELRRGYVAGDSKN 688
+KPG IV FAPA +TTEVKSVEMHHE+L +PGD ++RRG V GDSKN
Sbjct: 270 IKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKN 329
Query: 689 NPPKGAADFTAQVIVLNHPGQNLKRYTPVLDC 784
+PP G A FTAQVI+LNHPGQ Y+PVLDC
Sbjct: 330 DPPMGCASFTAQVIILNHPGQISAGYSPVLDC 361
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 149 bits (362), Expect = 3e-37
Identities = 65/87 (74%), Positives = 74/87 (85%)
Frame = +3
Query: 249 PSAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDK 428
P V FVP+SG+ GDNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPARPTDK
Sbjct: 183 PKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDK 242
Query: 429 PLRLPLQDVYKIGGIGTVPVGRVETGV 509
PLRLPLQDVYKIGGIGTVPVGRVETGV
Sbjct: 243 PLRLPLQDVYKIGGIGTVPVGRVETGV 269
Score = 135 bits (326), Expect = 8e-33
Identities = 64/84 (76%), Positives = 75/84 (89%)
Frame = +1
Query: 1 MITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPP 180
MITGTSQADCA+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T
Sbjct: 102 MITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--G 159
Query: 181 YSEPRFEEIKKEVSSYIKKIGYNP 252
+S+ RFEEI KE S++IKK+G+NP
Sbjct: 160 WSQARFEEIVKETSNFIKKVGFNP 183
Score = 123 bits (296), Expect = 3e-29
Identities = 56/92 (60%), Positives = 65/92 (70%)
Frame = +2
Query: 509 LKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDXXXXXXXXXXXXELRRGYVAGDSKN 688
+KPG IV FAPA +TTEVKSVEMHHE+L +PGD ++RRG V GDSKN
Sbjct: 270 IKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKN 329
Query: 689 NPPKGAADFTAQVIVLNHPGQNLKRYTPVLDC 784
+PP G A FTAQVI+LNHPGQ Y+PVLDC
Sbjct: 330 DPPMGCASFTAQVIILNHPGQISAGYSPVLDC 361
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 89.8 bits (213), Expect = 4e-19
Identities = 41/84 (48%), Positives = 59/84 (70%), Gaps = 1/84 (1%)
Frame = +1
Query: 1 MITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPP 180
MI G SQAD VL+++A GEFEAG + GQTREHA+LA T G+ L+V +NKMD
Sbjct: 333 MINGASQADIGVLVISARRGEFEAGFERGGQTREHAVLARTQGINHLVVVINKMDEPSVQ 392
Query: 181 YSEPRFEEIKKEVSSYIKKI-GYN 249
+SE R++E ++S +++++ GYN
Sbjct: 393 WSEERYKECVDKLSMFLRRVAGYN 416
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 79.4 bits (187), Expect = 5e-16
Identities = 41/83 (49%), Positives = 54/83 (65%), Gaps = 1/83 (1%)
Frame = +1
Query: 1 MITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPP 180
MI G S AD AVL+V + FE G +NGQTREHA L LG+ +++V VNK+D
Sbjct: 272 MIAGASSADFAVLVVDSSQNNFERGFLENGQTREHAYLLRALGISEIVVSVNKLDLMS-- 329
Query: 181 YSEPRFEEIKKEVSSY-IKKIGY 246
+SE RF+EIK VS + IK +G+
Sbjct: 330 WSEDRFQEIKNIVSDFLIKMVGF 352
Score = 50.4 bits (115), Expect = 3e-07
Identities = 34/87 (39%), Positives = 45/87 (51%), Gaps = 2/87 (2%)
Frame = +3
Query: 252 SAVAFVPISGWHGDNMLEPSTK--MPWFKGWQVERKEGKADGKCLIEALDAILPPARPTD 425
S V FVPIS G N+++ + W+KG L+ ALD ++PP +P
Sbjct: 355 SNVHFVPISAISGTNLIQKDSSDLYKWYKG------------PTLLSALDQLVPPEKPYR 402
Query: 426 KPLRLPLQDVYKIGGIGTVPVGRVETG 506
KPLRL + DVY+ TV GRVE G
Sbjct: 403 KPLRLSIDDVYRSPRSVTV-TGRVEAG 428
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 54.8 bits (126), Expect = 1e-08
Identities = 31/76 (40%), Positives = 46/76 (60%)
Frame = +1
Query: 1 MITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPP 180
MITG + D A+++V+A G+ QTREH LLA +GVKQ++V +NK+D EP
Sbjct: 133 MITGAATMDGAIIVVSATDGQMP-------QTREHLLLARQVGVKQIVVYINKVDMVEPD 185
Query: 181 YSEPRFEEIKKEVSSY 228
E E+++ +S Y
Sbjct: 186 MIELVEMEMRELLSEY 201
Score = 43.6 bits (98), Expect = 3e-05
Identities = 21/44 (47%), Positives = 29/44 (65%), Gaps = 1/44 (2%)
Frame = +3
Query: 378 LIEALDAILP-PARPTDKPLRLPLQDVYKIGGIGTVPVGRVETG 506
L+EA+D+ + P R TD P + ++DV+ I G GTV GRVE G
Sbjct: 234 LMEAVDSYITLPERKTDVPFLMAIEDVFSISGRGTVVTGRVERG 277
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 28.7 bits (61), Expect = 0.99
Identities = 18/77 (23%), Positives = 33/77 (42%)
Frame = +1
Query: 1 MITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPP 180
M+ G + D A+L++A QT EH + +K +I+ NK+D
Sbjct: 125 MLNGAAVMDAALLLIAGNES------CPQPQTSEHLAAIEIMQLKHIIILQNKVDLIRES 178
Query: 181 YSEPRFEEIKKEVSSYI 231
+E ++ I K + +
Sbjct: 179 AAEEHYQSILKFIKGTV 195
>SPAC17C9.06 |sam50||SAM complex subunit Sam50 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 475
Score = 28.7 bits (61), Expect = 0.99
Identities = 32/102 (31%), Positives = 44/102 (43%), Gaps = 15/102 (14%)
Frame = +1
Query: 256 LSLSCPFLDGTETTCWSLQPKCLGSRDGRWSVK-KAKLTENASLKLSMPSCHLPAP---- 420
+SL F+ G T+ +G +DGR S+ A + + SL +P P
Sbjct: 343 VSLCDRFMLGGSTSLRGFSEDRIGPKDGRDSLGGTAYMAFSMSLLFPLPKVDASKPFRLQ 402
Query: 421 ----------LTSPCVFPCKTYTKSVVLVPCPSAELKLVFET 516
LTSP PC TY KS++ PC S L LV+ T
Sbjct: 403 LFANAGGLSNLTSPN--PCGTY-KSILSKPCISTGLGLVYAT 441
>SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 835
Score = 28.3 bits (60), Expect = 1.3
Identities = 15/46 (32%), Positives = 19/46 (41%)
Frame = -1
Query: 418 GRAGGRMASRASMRHFPSALPSLRSTCHPLNQGILVEGSNMLSPCH 281
G GG S+ P+ LPS + P L E +ML CH
Sbjct: 545 GAGGGHAPDIISLVQNPNILPSSTNPTRPFTTNTLDEELDMLMVCH 590
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 4.0
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -1
Query: 655 QFLDGHVLYVETYIVSRYSFLESFV 581
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1242
Score = 26.2 bits (55), Expect = 5.3
Identities = 21/80 (26%), Positives = 29/80 (36%)
Frame = +1
Query: 403 CHLPAPLTSPCVFPCKTYTKSVVLVPCPSAELKLVFETRYHCCLCPRQHHY*SQICGDAP 582
C SP PCK T S+ V L + HC LC + + P
Sbjct: 11 CRCEGAPDSPLFHPCKC-TGSIRYVHQECLVEWLGHSKKTHCELCKAKFEFTKVYSESMP 69
Query: 583 RSSPRSCTWRQCKFQRKERV 642
R+ P + R+ K+RV
Sbjct: 70 RTIPFTILCRKLASTLKQRV 89
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 25.8 bits (54), Expect = 7.0
Identities = 10/39 (25%), Positives = 22/39 (56%)
Frame = -1
Query: 679 VTSNITTTQFLDGHVLYVETYIVSRYSFLESFVVHLHRF 563
+T ++ + L H+ +++ SR +FL ++H+H F
Sbjct: 1012 LTQSLQSFSHLSNHIEVLDSTRQSRLTFLCHLILHMHGF 1050
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 25.8 bits (54), Expect = 7.0
Identities = 19/63 (30%), Positives = 27/63 (42%)
Frame = +3
Query: 318 MPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRV 497
+P K V K GK + L E + I PP + PLR L D + G + + R+
Sbjct: 206 IPMSKPILVSSKTGKNVEQILPEIIQKIPPPKGSENAPLRCLLIDSWYNSYQGVIGLVRI 265
Query: 498 ETG 506
G
Sbjct: 266 MEG 268
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,472,539
Number of Sequences: 5004
Number of extensions: 76506
Number of successful extensions: 252
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 230
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 248
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 379359666
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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