BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10412 (849 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 55 1e-08 SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 48 1e-06 SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 35 0.013 SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 28 1.5 SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein Msa1|Schizosa... 27 2.5 SPCC584.16c |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 27 4.4 SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac... 27 4.4 SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase Ubp16|Schizo... 26 7.8 >SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 54.8 bits (126), Expect = 1e-08 Identities = 33/88 (37%), Positives = 49/88 (55%), Gaps = 6/88 (6%) Frame = +2 Query: 257 KDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQLLTLFANRMSGGSDE 424 +DG I N+L SLG+ + EL +M+ E +G I+FT+ LT+ A +M +D Sbjct: 25 QDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMK-DTDN 83 Query: 425 DDVVINAFKTFDEEGK--IDSERLRHAL 502 ++ V AFK FD++G I E L H L Sbjct: 84 EEEVREAFKVFDKDGNGYITVEELTHVL 111 >SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 184 Score = 48.4 bits (110), Expect = 1e-06 Identities = 29/83 (34%), Positives = 43/83 (51%), Gaps = 2/83 (2%) Frame = +2 Query: 260 DGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANRMSGGSDEDDVVI 439 DG IG+ D++ SL + ASE ++ M + PIN LT + + S +D ++ Sbjct: 62 DGNIGREDVKTMLTSLNQDASEDSINHMFESINPPINLAAFLTAMGSMLCRISPRND-LL 120 Query: 440 NAFKTFD--EEGKIDSERLRHAL 502 AF TFD + GKI +R AL Sbjct: 121 EAFSTFDDTQSGKIPISTMRDAL 143 Score = 30.3 bits (65), Expect = 0.36 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = +3 Query: 171 KRTGSNVFSMFSQKQVAEFKEAFQLMD 251 KR S F+ + Q+ E KEAF L+D Sbjct: 32 KRASSGAFAQLTSSQIQELKEAFALLD 58 >SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces pombe|chr 1|||Manual Length = 143 Score = 35.1 bits (77), Expect = 0.013 Identities = 21/86 (24%), Positives = 45/86 (52%), Gaps = 2/86 (2%) Frame = +2 Query: 257 KDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANRMSGGSDEDDVV 436 KDG+I + + + SLG ++ EL ++ E I+ + ++ +N++ E++ Sbjct: 22 KDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVSNKLRETESEEE-Y 80 Query: 437 INAFKTFDEE--GKIDSERLRHALMT 508 I AF+ FD++ G I++ + + T Sbjct: 81 IKAFRVFDKDNSGYIETAKFADYMKT 106 >SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pombe|chr 1|||Manual Length = 141 Score = 28.3 bits (60), Expect = 1.5 Identities = 12/28 (42%), Positives = 18/28 (64%) Frame = +2 Query: 263 GIIGKNDLRATFDSLGRLASEKELDEMV 346 G+IG +LR SLG S +E+DE++ Sbjct: 92 GMIGVGELRYVLTSLGEKLSNEEMDELL 119 >SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein Msa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 533 Score = 27.5 bits (58), Expect = 2.5 Identities = 25/71 (35%), Positives = 33/71 (46%) Frame = -1 Query: 444 ALITTSSSSEPPDMRLANRVKS*VKLMGPEASPTISSSSFSDASLPRESKVARKSFLPMM 265 A I S SS+PP R S V+ A ++S+ FS P ES + S LP + Sbjct: 272 APIPFSVSSDPPSTM--GRSNSAVQSPSYFAHSLVNSTEFST---PNESLSSLPSILPSI 326 Query: 264 PSLCDPLAGMP 232 PSL A +P Sbjct: 327 PSLESGKAELP 337 >SPCC584.16c |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 231 Score = 26.6 bits (56), Expect = 4.4 Identities = 15/36 (41%), Positives = 18/36 (50%) Frame = -1 Query: 339 SSSSFSDASLPRESKVARKSFLPMMPSLCDPLAGMP 232 SSSS S +S P + +RK P MP L L P Sbjct: 3 SSSSSSGSSSPASKRSSRKRQTPPMPELPSFLLAFP 38 >SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 882 Score = 26.6 bits (56), Expect = 4.4 Identities = 24/56 (42%), Positives = 31/56 (55%), Gaps = 2/56 (3%) Frame = -1 Query: 420 SEPPDMRLANRVK-S*VKL-MGPEASPTISSSSFSDASLPRESKVARKSFLPMMPS 259 S P R+A+ +K S VKL + P+A + SS+ S ASL ES R S P PS Sbjct: 542 SRPRLPRVASPLKTSPVKLAVTPQAPSPLPSSNPSQASLTEESLSTRSS--PTKPS 595 >SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase Ubp16|Schizosaccharomyces pombe|chr 3|||Manual Length = 457 Score = 25.8 bits (54), Expect = 7.8 Identities = 11/33 (33%), Positives = 22/33 (66%), Gaps = 1/33 (3%) Frame = -1 Query: 354 ASPTISSSSFSDASLPRESKVARKSF-LPMMPS 259 +SP++ +++ + P++S V+RKSF P P+ Sbjct: 35 SSPSVPEGTYTVLNNPKQSTVSRKSFSAPTSPT 67 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,672,845 Number of Sequences: 5004 Number of extensions: 45348 Number of successful extensions: 159 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 145 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 158 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 420459900 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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