Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV10401
(768 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 169 2e-42
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 108 5e-24
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 107 8e-24
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 107 8e-24
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 52 3e-07
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 34 0.091
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 34 0.091
At5g36905.1 68418.m04423 RNase H domain-containing protein low s... 29 2.6
At5g61930.1 68418.m07774 expressed protein contains Pfam PF05634... 29 3.4
At1g78140.1 68414.m09106 methyltransferase-related similar to Pr... 29 3.4
At1g67560.1 68414.m07697 lipoxygenase family protein similar to ... 29 3.4
At5g06805.1 68418.m00769 reverse transcriptase-related low simil... 28 7.9
At2g35490.1 68415.m04347 plastid-lipid associated protein PAP, p... 28 7.9
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 169 bits (410), Expect = 2e-42
Identities = 87/171 (50%), Positives = 115/171 (67%), Gaps = 2/171 (1%)
Frame = +2
Query: 254 IKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLL 433
IKDSVVAGFQWA+KEG +AEEN+RG+ F + DV LH+DAIHRGGGQ+IPT RR +YA +
Sbjct: 660 IKDSVVAGFQWASKEGPLAEENMRGICFEVCDVVLHSDAIHRGGGQVIPTARRVIYASQI 719
Query: 434 TAQPRLMEPVYLCEIQCPEVAVGGI-TVC*TDVVVTFSKSPRWQVHLCSL*RPTYLSMSR 610
TA+PRL+EPVY+ EIQ PE A+GGI +V F + R L ++ YL +
Sbjct: 720 TAKPRLLEPVYMVEIQAPEGALGGIYSVLNQKRGHVFEEMQRPGTPLYNI--KAYLPVVE 777
Query: 611 SVLLPICV-PTPADKAFPQCVFDHWQVLPGDPCEPQSKPYNVVQETRKRKG 760
S + + +AFPQCVFDHW+++ DP EP ++ +V + RKRKG
Sbjct: 778 SFGFSSQLRAATSGQAFPQCVFDHWEMMSSDPLEPGTQASVLVADIRKRKG 828
Score = 127 bits (307), Expect = 7e-30
Identities = 62/123 (50%), Positives = 86/123 (69%), Gaps = 5/123 (4%)
Frame = +3
Query: 6 LSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWC 185
+SKSPNKHNRL+M+A+PM +GL E ID+GR+ PRDD K R++ L E++ +D A+KIW
Sbjct: 577 MSKSPNKHNRLYMEARPMEEGLAEAIDDGRIGPRDDPKIRSKILAEEFGWDKDLAKKIWA 636
Query: 186 FGPEGTGPNILVDCSKGVQYLNELRTLL-----WLDSSGPLRKELWLKRICVVFDSTSMM 350
FGPE TGPN++VD KGVQYLNE++ + W GPL +E ++ IC F+ ++
Sbjct: 637 FGPETTGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGPLAEEN-MRGIC--FEVCDVV 693
Query: 351 *HS 359
HS
Sbjct: 694 LHS 696
Score = 73.3 bits (172), Expect = 2e-13
Identities = 32/54 (59%), Positives = 41/54 (75%)
Frame = +1
Query: 499 GWYHGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQGLP 660
G + VLN++RGHVFEE Q GTP++ +KAYLPV ESFGF++ LR+ T GQ P
Sbjct: 742 GGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVVESFGFSSQLRAATSGQAFP 795
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 108 bits (259), Expect = 5e-24
Identities = 62/155 (40%), Positives = 81/155 (52%), Gaps = 3/155 (1%)
Frame = +2
Query: 254 IKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLL 433
+KDS+V GFQW A+EG + +E +R V+F I D + + +HRG GQ+IPT RR Y+ L
Sbjct: 762 VKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFL 821
Query: 434 TAQPRLMEPVYLCEIQCPEVAVGGI-TVC--*TDVVVTFSKSPRWQVHLCSL*RPTYLSM 604
A PRLMEPVY EIQ P V I TV V + P ++ P S
Sbjct: 822 MATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGYVTSDVPQPGTPAYIVKAFLPVIESF 881
Query: 605 SRSVLLPICVPTPADKAFPQCVFDHWQVLPGDPCE 709
L +AF VFDHW ++PGDP +
Sbjct: 882 GFETDLRY---HTQGQAFCLSVFDHWAIVPGDPLD 913
Score = 68.1 bits (159), Expect = 6e-12
Identities = 31/74 (41%), Positives = 46/74 (62%)
Frame = +3
Query: 3 CLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIW 182
C +++PNK N+L M A+P+ GL EDI+ G V+ + + KY++D+ AR IW
Sbjct: 674 CFAETPNKKNKLTMIAEPLDRGLAEDIENGVVSIDWNRVQLGDFFRTKYDWDLLAARSIW 733
Query: 183 CFGPEGTGPNILVD 224
FGP+ G NIL+D
Sbjct: 734 AFGPDKQGTNILLD 747
Score = 58.8 bits (136), Expect = 4e-09
Identities = 28/46 (60%), Positives = 34/46 (73%)
Frame = +1
Query: 514 VLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQ 651
VL+RRRG+V + GTP +IVKA+LPV ESFGF DLR +T GQ
Sbjct: 849 VLSRRRGYVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQ 894
>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 107 bits (257), Expect = 8e-24
Identities = 62/155 (40%), Positives = 81/155 (52%), Gaps = 3/155 (1%)
Frame = +2
Query: 254 IKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLL 433
+KDS+V GFQW A+EG + +E +R V+F I D + + +HRG GQ+IPT RR Y+ L
Sbjct: 776 VKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFL 835
Query: 434 TAQPRLMEPVYLCEIQCPEVAVGGI-TVC--*TDVVVTFSKSPRWQVHLCSL*RPTYLSM 604
A PRLMEPVY EIQ P V I TV V + P ++ P S
Sbjct: 836 MATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESF 895
Query: 605 SRSVLLPICVPTPADKAFPQCVFDHWQVLPGDPCE 709
L +AF VFDHW ++PGDP +
Sbjct: 896 GFETDLRY---HTQGQAFCLSVFDHWAIVPGDPLD 927
Score = 73.3 bits (172), Expect = 2e-13
Identities = 32/74 (43%), Positives = 48/74 (64%)
Frame = +3
Query: 3 CLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIW 182
C +++PNK N++ M A+P+ GL EDI+ G V+ + K + KY++D+ AR IW
Sbjct: 688 CFAETPNKKNKITMIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAARSIW 747
Query: 183 CFGPEGTGPNILVD 224
FGP+ GPNIL+D
Sbjct: 748 AFGPDKQGPNILLD 761
Score = 61.3 bits (142), Expect = 7e-10
Identities = 29/46 (63%), Positives = 34/46 (73%)
Frame = +1
Query: 514 VLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQ 651
VL+RRRGHV + GTP +IVKA+LPV ESFGF DLR +T GQ
Sbjct: 863 VLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQ 908
>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 107 bits (257), Expect = 8e-24
Identities = 62/155 (40%), Positives = 81/155 (52%), Gaps = 3/155 (1%)
Frame = +2
Query: 254 IKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLL 433
+KDS+V GFQW A+EG + +E +R V+F I D + + +HRG GQ+IPT RR Y+ L
Sbjct: 776 VKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFL 835
Query: 434 TAQPRLMEPVYLCEIQCPEVAVGGI-TVC--*TDVVVTFSKSPRWQVHLCSL*RPTYLSM 604
A PRLMEPVY EIQ P V I TV V + P ++ P S
Sbjct: 836 MATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESF 895
Query: 605 SRSVLLPICVPTPADKAFPQCVFDHWQVLPGDPCE 709
L +AF VFDHW ++PGDP +
Sbjct: 896 GFETDLRY---HTQGQAFCLSVFDHWAIVPGDPLD 927
Score = 73.3 bits (172), Expect = 2e-13
Identities = 32/74 (43%), Positives = 48/74 (64%)
Frame = +3
Query: 3 CLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIW 182
C +++PNK N++ M A+P+ GL EDI+ G V+ + K + KY++D+ AR IW
Sbjct: 688 CFAETPNKKNKITMIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAARSIW 747
Query: 183 CFGPEGTGPNILVD 224
FGP+ GPNIL+D
Sbjct: 748 AFGPDKQGPNILLD 761
Score = 61.3 bits (142), Expect = 7e-10
Identities = 29/46 (63%), Positives = 34/46 (73%)
Frame = +1
Query: 514 VLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQ 651
VL+RRRGHV + GTP +IVKA+LPV ESFGF DLR +T GQ
Sbjct: 863 VLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQ 908
>At3g22980.1 68416.m02898 elongation factor Tu family protein similar
to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 52.4 bits (120), Expect = 3e-07
Identities = 23/50 (46%), Positives = 32/50 (64%)
Frame = +1
Query: 499 GWYHGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGG 648
G + VL+RRR + +E G+ +F V AY+PV+ESFGF +LR T G
Sbjct: 891 GPMYAVLSRRRARILKEEMQEGSSLFTVHAYVPVSESFGFADELRKGTSG 940
Score = 41.9 bits (94), Expect = 5e-04
Identities = 26/88 (29%), Positives = 43/88 (48%), Gaps = 9/88 (10%)
Frame = +2
Query: 254 IKDSVVAGFQWAAKEGVMAEENLRGVRFNIYD-----VTLHTDAIHRGG---GQIIPTTR 409
++ S+V+GFQ A G + +E + G+ F I + TD G GQ++ +
Sbjct: 801 LESSIVSGFQLATASGPLCDEPMWGLAFTIESHLAPAEDVETDKPENFGIFTGQVMTAVK 860
Query: 410 RCLYACLLTAQPRLMEPVYLCEIQ-CPE 490
A +L PR++E +Y CE+ PE
Sbjct: 861 DACRAAVLQTNPRIVEAMYFCELNTAPE 888
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 34.3 bits (75), Expect = 0.091
Identities = 17/52 (32%), Positives = 28/52 (53%)
Frame = +1
Query: 499 GWYHGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQG 654
G G +N+R+G + Q ++ A +P+N FG++ LRS T G+G
Sbjct: 674 GTVAGDINKRKGIIVGNDQEGDDS--VITANVPLNNMFGYSTSLRSMTQGKG 723
Score = 29.1 bits (62), Expect = 3.4
Identities = 21/86 (24%), Positives = 37/86 (43%), Gaps = 3/86 (3%)
Frame = +2
Query: 239 SVPQ*IKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCL 418
++P ++ GF+ AA G + + +R + L A H + +
Sbjct: 589 AIPSGFIPAIEKGFKEAANSGSLIGHPVENLR-----IVLTDGASHAVDSSELAFKMAAI 643
Query: 419 YA---CLLTAQPRLMEPVYLCEIQCP 487
YA C A+P ++EPV L E++ P
Sbjct: 644 YAFRLCYTAARPVILEPVMLVELKVP 669
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 34.3 bits (75), Expect = 0.091
Identities = 17/52 (32%), Positives = 28/52 (53%)
Frame = +1
Query: 499 GWYHGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQG 654
G G +N+R+G + Q ++ A +P+N FG++ LRS T G+G
Sbjct: 674 GTVAGDINKRKGIIVGNDQEGDDS--VITANVPLNNMFGYSTSLRSMTQGKG 723
Score = 29.1 bits (62), Expect = 3.4
Identities = 21/86 (24%), Positives = 37/86 (43%), Gaps = 3/86 (3%)
Frame = +2
Query: 239 SVPQ*IKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCL 418
++P ++ GF+ AA G + + +R + L A H + +
Sbjct: 589 AIPSGFIPAIEKGFKEAANSGSLIGHPVENLR-----IVLTDGASHAVDSSELAFKMAAI 643
Query: 419 YA---CLLTAQPRLMEPVYLCEIQCP 487
YA C A+P ++EPV L E++ P
Sbjct: 644 YAFRLCYTAARPVILEPVMLVELKVP 669
>At5g36905.1 68418.m04423 RNase H domain-containing protein low
similarity to reverse transcriptase [Arabidopsis
thaliana] GI:976278; contains Pfam profile PF00075:
RNase H
Length = 633
Score = 29.5 bits (63), Expect = 2.6
Identities = 24/77 (31%), Positives = 36/77 (46%)
Frame = -2
Query: 242 LNSFGAIDQDVGAGTLGAKAPNLTGFGNIVFVLFCKIPSASLEVIAGIYATLINVLWQTI 63
LN+ GA + G T G + G + FVL I SA L + G+Y L ++ W+
Sbjct: 462 LNTDGASRGNPGLATAGGVVRDGEGQWCVGFVLNIGICSAPLAELWGVYYGL-HIAWERG 520
Query: 62 RHGLSLHEQTVVLVGRL 12
L L + ++VG L
Sbjct: 521 IRRLELEVDSTLVVGFL 537
>At5g61930.1 68418.m07774 expressed protein contains Pfam PF05634:
Arabidopsis thaliana protein of unknown function
(DUF794)
Length = 340
Score = 29.1 bits (62), Expect = 3.4
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = -2
Query: 740 PVQRCRACSEVRTGLQG 690
PV RCR C+EV G QG
Sbjct: 62 PVHRCRLCAEVHIGKQG 78
>At1g78140.1 68414.m09106 methyltransferase-related similar to
Probable delta(24)-sterol C-methyltransferase
(Swiss-Prot:O14321) [Schizosaccharomyces pombe]; similar
to C5-O-methyltransferase (GI:5921167) [Streptomyces
avermitilis]; similar to S-adenosyl-methionine-sterol-C-
methyltransferase (GI:3560474) [Nicotiana tabacum]
Length = 355
Score = 29.1 bits (62), Expect = 3.4
Identities = 18/58 (31%), Positives = 30/58 (51%), Gaps = 1/58 (1%)
Frame = +3
Query: 588 LPTCQ*VVRFYC-RFAFQHRRTRPSRSAYSTIGRSSLETRANLRASPTTLYRKRERGK 758
LPTC + R + ++A Q R SRSA+++ S+ET +N + + RG+
Sbjct: 15 LPTCFSLSRLHSVKYAAQRRVVFVSRSAHASSASVSVETNSNSNVDFVIEKKDKNRGE 72
>At1g67560.1 68414.m07697 lipoxygenase family protein similar to
13-lipoxygenase GB:CAA65269 [Solanum tuberosum],
gi:1654140 [Lycopersicon esculentum]
Length = 917
Score = 29.1 bits (62), Expect = 3.4
Identities = 18/56 (32%), Positives = 28/56 (50%), Gaps = 5/56 (8%)
Frame = +3
Query: 9 SKSPNKHNRLFMKAQPM-----PDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDV 161
SK+ N R+ ++QP PDG+ E ++ V+ R D K + Y+YDV
Sbjct: 204 SKNDNPQARIIFRSQPCLPSETPDGIKELREKDLVSVRGDGKGERKPHERIYDYDV 259
>At5g06805.1 68418.m00769 reverse transcriptase-related low
similarity to reverse transcriptase [Arabidopsis
thaliana] GI:976278
Length = 594
Score = 27.9 bits (59), Expect = 7.9
Identities = 21/66 (31%), Positives = 32/66 (48%), Gaps = 1/66 (1%)
Frame = -2
Query: 242 LNSFGAIDQDVGAGTLGAKAPNLTGFGNIVFVLFCKIPSASLEVIAGIYATLINVLWQTI 63
+N+ GA + G T G + G + F L + SA L + G+Y L+ V W+ +
Sbjct: 505 MNTDGASHGNPGQATAGGVIRDEHGSWLVGFALNIGVCSAPLAELWGVYYGLV-VAWERV 563
Query: 62 RH-GLS 48
R GLS
Sbjct: 564 RDWGLS 569
>At2g35490.1 68415.m04347 plastid-lipid associated protein PAP,
putative similar to plastid-lipid associated protein
PAP3 [Brassica rapa] GI:14248552; contains Pfam profile
PF04755: PAP_fibrillin
Length = 376
Score = 27.9 bits (59), Expect = 7.9
Identities = 12/22 (54%), Positives = 13/22 (59%)
Frame = +2
Query: 668 VFDHWQVLPGDPCEPQSKPYNV 733
V D W PGD EP S+P NV
Sbjct: 71 VTDEWGEKPGDANEPDSQPDNV 92
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,189,585
Number of Sequences: 28952
Number of extensions: 458977
Number of successful extensions: 1207
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 1158
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1206
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1721869952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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