BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV10399 (600 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1B2.05 |mcm5|nda4, SPAC3F10.01|MCM complex subunit Mcm5|Schi... 62 8e-11 SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosacc... 35 0.008 SPCC16A11.17 |cdc21|mcm4, SPCC24B10.01|MCM complex subunit Cdc21... 29 0.39 SPBC211.04c |mcm6|mis5|MCM complex subunit Mcm6 |Schizosaccharom... 27 2.1 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 4.8 >SPAC1B2.05 |mcm5|nda4, SPAC3F10.01|MCM complex subunit Mcm5|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 61.7 bits (143), Expect = 8e-11 Identities = 34/85 (40%), Positives = 44/85 (51%) Frame = +1 Query: 4 AAGELPLTTLRRYSAYXXXXXXXXXXXXXXXXXXXXYVLMRTGALQHERQADKRLYIPIT 183 A GE+P RRY Y +V +R Q E+ ++ R IPIT Sbjct: 543 AIGEIPFDKFRRYINYCRHKCAPNLDAEAAEKLSSQFVAIRKRVHQSEQDSNSRSTIPIT 602 Query: 184 VRQLEAIVHISESLAKMQLQPFATK 258 VRQLEAI+ I+ESLAKM L P A++ Sbjct: 603 VRQLEAIIRITESLAKMSLSPIASE 627 Score = 41.1 bits (92), Expect = 1e-04 Identities = 25/90 (27%), Positives = 47/90 (52%), Gaps = 1/90 (1%) Frame = +3 Query: 234 AVATVCDEAHVNEALRLFQVSTLDAAMTGSLAGAEGFTTEEDHEMLTRVEKQLKRRFAVG 413 +++ + EAH EA+RLF STL AA S E E + ++E L++R +G Sbjct: 620 SLSPIASEAHATEAIRLFLTSTLAAATQSS---------PEVTEEVKKIEASLRKRLPIG 670 Query: 414 SQVSEQTIIQDFLR-QKYPERAILKVIHMM 500 Q S + +I++++ Y + A+ + ++ Sbjct: 671 FQASYRMLIREYVNGHGYSQHALEMALQIL 700 >SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 830 Score = 35.1 bits (77), Expect = 0.008 Identities = 15/25 (60%), Positives = 19/25 (76%) Frame = +1 Query: 175 PITVRQLEAIVHISESLAKMQLQPF 249 PITVR LE+ + +SE+ AKMQL F Sbjct: 768 PITVRHLESAIRLSEAFAKMQLSEF 792 >SPCC16A11.17 |cdc21|mcm4, SPCC24B10.01|MCM complex subunit Cdc21|Schizosaccharomyces pombe|chr 3|||Manual Length = 911 Score = 29.5 bits (63), Expect = 0.39 Identities = 15/32 (46%), Positives = 23/32 (71%) Frame = +1 Query: 148 RQADKRLYIPITVRQLEAIVHISESLAKMQLQ 243 R ++KR I T RQLE+++ +SE+ AKM L+ Sbjct: 762 RASEKR--ITATTRQLESMIRLSEAHAKMHLR 791 >SPBC211.04c |mcm6|mis5|MCM complex subunit Mcm6 |Schizosaccharomyces pombe|chr 2|||Manual Length = 892 Score = 27.1 bits (57), Expect = 2.1 Identities = 10/18 (55%), Positives = 17/18 (94%) Frame = +1 Query: 178 ITVRQLEAIVHISESLAK 231 ITVRQLE+++ +SE++A+ Sbjct: 696 ITVRQLESMIRLSEAIAR 713 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 25.8 bits (54), Expect = 4.8 Identities = 17/44 (38%), Positives = 22/44 (50%) Frame = -2 Query: 461 FLSQEVLNDCLFRNLRAHSEPAL*LFLDASQHFVIFFCCKTFSS 330 F S VLND LFRN +S+P L + +F CC F + Sbjct: 1130 FPSPSVLNDTLFRNRINNSKP---LGVFRIHDPSLFACCYQFGA 1170 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,720,488 Number of Sequences: 5004 Number of extensions: 28204 Number of successful extensions: 69 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 65 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 68 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 262236260 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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